Restriction map for
pFN21AB0996
(Circular) (Six-base) MAPSORT of: pFN21AB0996.seq Check: 6389 from: 1 to: 6100
pFN21AB0996 6100 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2057
Size: 6100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2635 3443 1937
Size: 698 808 4594
Fragments arranged by size:
4594 808 698
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 2687 4163 5547 5815 1632
Size: 833 222 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 833 268 222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6100
AlwNI CAG_nnn'CTG
Cuts at: 2109 2479 2629 2798 3232 3843 5415 2109
Size: 370 150 169 434 611 1572 2794
Fragments arranged by size:
2794 1572 611 434 370 169 150
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3243 3409 3635 3787 5896 1493
Size: 1750 166 226 152 2109 1697
Fragments arranged by size:
2109 1750 1697 226 166 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 3425 1870
Size: 73 1482 4545
Fragments arranged by size:
4545 1482 73
BamHI G'GATC_C
Cuts at: 2893 3430 3797 2893
Size: 537 367 5196
Fragments arranged by size:
5196 537 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 3421 4876 618
Size: 325 217 627 569 1065 1455 1842
Fragments arranged by size:
1842 1455 1065 627 569 325 217
BanII G_rGCy'C
Cuts at: 1391 2197 2723 3419 1391
Size: 806 526 696 4072
Fragments arranged by size:
4072 806 696 526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 2463 3046 928
Size: 971 501 63 583 3982
Fragments arranged by size:
3982 971 583 501 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2948 3283 3992 5202 2948
Size: 335 709 1210 3846
Fragments arranged by size:
3846 1210 709 335
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 2722 3517 2722
Size: 795 5305
Bme1580I G_kGCm'C
Cuts at: 1790 2359 2376 3119 4161 5317 1790
Size: 569 17 743 1042 1156 2573
Fragments arranged by size:
2573 1156 1042 743 569 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2179 3831
Size: 416 53 83 399 24 658 104 1652
Cuts at: 3831 4789 442
Size: 958 1753
Fragments arranged by size:
1753 1652 958 658 416 399 104 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2546 2620 2627 2723 4760 1239
Size: 1169 138 74 7 96 2037 2579
Fragments arranged by size:
2579 2037 1169 138 96 74 7
Bpu10I CC'TnA_GC
Cuts at: 2063 2063
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2666 4225 5090 5388 2666
Size: 1559 865 298 3378
Fragments arranged by size:
3378 1559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3907 4289 4924 275
Size: 53 83 186 936 2374 382 635 1451
Fragments arranged by size:
2374 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2541 3074 4533 4931 5205 5352 5763 2541
Size: 533 1459 398 274 147 411 2878
Fragments arranged by size:
2878 1459 533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5875
Fragments arranged by size:
5875 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6100
BseYI C'CCAG_C
Cuts at: 2233 5303 5799 2233
Size: 3070 496 2534
Fragments arranged by size:
3070 2534 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3128 1163
Size: 573 1392 4135
Fragments arranged by size:
4135 1392 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4161 4246 5317 4161
Size: 85 1071 4944
Fragments arranged by size:
4944 1071 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2359 2376 2723 3119 3419 4161
Size: 399 407 162 17 347 396 300 742
Cuts at: 4161 4246 5317 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 407 399 396 347 300
162 85 17
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 3455 844
Size: 1213 1398 3489
Fragments arranged by size:
3489 1398 1213
BsrBI CCG'CTC
Cuts at: 1394 1823 2353 3987 1394
Size: 429 530 1634 3507
Fragments arranged by size:
3507 1634 530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2496 2680 2833 4595 4769 65
Size: 1183 1248 184 153 1762 174 1396
Fragments arranged by size:
1762 1396 1248 1183 184 174 153
BsrFI r'CCGG_y
Cuts at: 1700 1810 4744 4931 1700
Size: 110 2934 187 2869
Fragments arranged by size:
2934 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 2949 4160 5172 2949
Size: 1211 1012 3877
Fragments arranged by size:
3877 1211 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3232 794
Size: 2438 3662
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2893 3430 3797 4182 4199 5651 5737 5749
Size: 921 537 367 385 17 1452 86 12
Cuts at: 5749 6095 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 921 537 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3034 3158 3034
Size: 124 5976
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4609
Fragments arranged by size:
4609 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 3616 4410 4430 3616
Size: 794 20 5286
Fragments arranged by size:
5286 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2368 2403 529
Size: 35 1804 35 4226
Fragments arranged by size:
4226 1804 35 35
DraI TTT'AAA
Cuts at: 2137 3404 2137
Size: 1267 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065
Size: 54 1425 50 275 18 1717 889 1629
Cuts at: 6065 8
Size: 43
Fragments arranged by size:
1717 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 2663 4030 1353
Size: 1224 86 1367 3423
Fragments arranged by size:
3423 1367 1224 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2465 2546 2620 2627 2687 2723
Size: 393 776 57 81 74 7 60 36
Cuts at: 2723 4163 4760 5547 5815 1239
Size: 1440 597 787 268 1524
Fragments arranged by size:
1524 1440 787 776 597 393 268 81
74 60 57 36 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 3390 2576
Size: 814 5286
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 3343 2860
Size: 483 5617
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 2347 5247 1678
Size: 669 2900 2531
Fragments arranged by size:
2900 2531 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2523 2555 2878 2910 3125
Size: 32 135 32 951 32 323 32 215
Cuts at: 3125 3157 4741 4773 4815 4847 1373
Size: 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1584 951 323 215 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2837 748
Size: 2089 4011
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2242 5214 5398 1305
Size: 937 2972 184 2007
Fragments arranged by size:
2972 2007 937 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 3122 3219 4058 4417 4576 518
Size: 648 1323 633 97 839 359 159 2042
Fragments arranged by size:
2042 1323 839 648 633 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2233 3516 4193 5341 5586 1606
Size: 627 1283 677 1148 245 2120
Fragments arranged by size:
2120 1283 1148 677 627 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4609
Fragments arranged by size:
4609 939 552
NdeI CA'TA_TG
Cuts at: 387 2205 387
Size: 1818 4282
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3458 5003 1132
Size: 97 2229 1545 2229
Fragments arranged by size:
2229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6100
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2695 1108
Size: 1587 4513
PfoI T'CCnGG_A
Cuts at: 2694 2984 2694
Size: 290 5810
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2907 2939 4169 4201 5682 5714 2907
Size: 32 1230 32 1481 32 3293
Fragments arranged by size:
3293 1481 1230 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3282 3670 3282
Size: 388 5712
PstI C_TGCA'G
Cuts at: 830 2742 3452 830
Size: 1912 710 3478
Fragments arranged by size:
3478 1912 710
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2233 2233
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2663 2663
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2738 3448 4583 5264 5455 826
Size: 219 1693 710 1135 681 191 1471
Fragments arranged by size:
1693 1471 1135 710 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 4204 5105 5367 820
Size: 197 926 738 1523 901 262 1553
Fragments arranged by size:
1553 1523 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 3368 4024 5
Size: 47 3316 656 2081
Fragments arranged by size:
3316 2081 656 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2805 3539 513
Size: 552 939 801 734 3074
Fragments arranged by size:
3074 939 801 734 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2975 4127 4144 4297 4482 4927 325
Size: 1255 1395 1152 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 1152 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 6058 96
Size: 274 80 33 51 1443 2369 1712 138
Fragments arranged by size:
2369 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6060 6092 6060
Size: 32 6068
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2032 2230 2032
Size: 198 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 2919 4229 2919
Size: 1310 4790
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI