Restriction map for
pFN21AB0993
(Circular) (Six-base) MAPSORT of: pFN21AB0993.seq Check: 3754 from: 1 to: 5539
pFN21AB0993 5539 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2201 2621 3602 4986 5254 1632
Size: 569 420 981 1384 268 1917
Fragments arranged by size:
1917 1384 981 569 420 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5539
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaI G_GGCC'C
Cuts at: 2099 2099
Size: 5539
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2576 2848 3074 3226 5335 1493
Size: 1083 272 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2777 2864 1870
Size: 73 155 679 87 4545
Fragments arranged by size:
4545 679 155 87 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2860 4315 618
Size: 325 217 627 1073 1455 1842
Fragments arranged by size:
1842 1455 1073 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2025 2099 2838 2858 1391
Size: 634 74 739 20 4072
Fragments arranged by size:
4072 739 634 74 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2030 2571 928
Size: 971 131 541 3896
Fragments arranged by size:
3896 971 541 131
BbvCI CC'TCA_GC
Cuts at: 2115 2115
Size: 5539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2453 2487 3730 3764 1903
Size: 34 516 34 1243 34 3678
Fragments arranged by size:
3678 1243 516 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2099 3600 4756 1790
Size: 309 1501 1156 2573
Fragments arranged by size:
2573 1501 1156 309
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2137 3270 4228
Size: 416 53 83 399 24 720 1133 958
Cuts at: 4228 442
Size: 1753
Fragments arranged by size:
1753 1133 958 720 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 4199 1239
Size: 1041 1919 2579
Fragments arranged by size:
2579 1919 1041
Bpu10I CC'TnA_GC
Cuts at: 2019 2115 2019
Size: 96 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2474 2780 3664 4529 4827 2474
Size: 306 884 865 298 3186
Fragments arranged by size:
3186 884 865 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 2381 2760 4202 882
Size: 617 801 81 379 1442 2219
Fragments arranged by size:
2219 1442 801 617 379 81
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3330 493
Size: 711 1392 734 2702
Fragments arranged by size:
2702 1392 734 711
BsaBI GATnn'nnATC
Cuts at: 2441 5533 2441
Size: 3092 2447
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 1178
Size: 30 165 30 765 30 4519
Fragments arranged by size:
4519 765 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2540 2795 1186
Size: 946 408 255 3930
Fragments arranged by size:
3930 946 408 255
BseYI C'CCAG_C
Cuts at: 2092 2141 2749 2771 4742 5238 2092
Size: 49 608 22 1971 496 2393
Fragments arranged by size:
2393 1971 608 496 49 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 1163
Size: 573 588 4378
Fragments arranged by size:
4378 588 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2838 3600 3685 4756 2838
Size: 762 85 1071 3621
Fragments arranged by size:
3621 1071 762 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2099 2838 2858 3600 3685 4756
Size: 399 235 74 739 20 742 85 1071
Cuts at: 4756 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 739 399 235 85 74
20
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2201 2354 4183 4370 1700
Size: 110 270 121 153 1829 187 2869
Fragments arranged by size:
2869 1829 270 187 153 121 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2623
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2869 3236 3621 3638 5090 5176 5188 5534
Size: 897 367 385 17 1452 86 12 346
Cuts at: 5534 1972
Size: 1977
Fragments arranged by size:
1977 1452 897 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 2504 2639 3055 3849 3869 2504
Size: 135 416 794 20 4174
Fragments arranged by size:
4174 794 416 135 20
ClaI AT'CG_AT
Cuts at: 1554 2365 1554
Size: 811 4728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2843 2843
Size: 5539
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 2684 3469 1353
Size: 1158 173 785 3423
Fragments arranged by size:
3423 1158 785 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2836 2836
Size: 5539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2280 2621 3602 4199 4986 5254
Size: 393 569 79 341 981 597 787 268
Cuts at: 5254 1239
Size: 1524
Fragments arranged by size:
1524 981 787 597 569 393 341 268
79
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2342
Size: 5539
EcoRI G'AATT_C
Cuts at: 2576 2848 3226 2576
Size: 272 378 4889
Fragments arranged by size:
4889 378 272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2206 2299 2206
Size: 93 5446
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 4686 1678
Size: 3008 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 2168 2200 2621
Size: 32 135 32 440 32 124 32 421
Cuts at: 2621 2653 4180 4212 4254 4286 1373
Size: 32 1527 32 42 32 2626
Fragments arranged by size:
2626 1527 440 421 135 124 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2259 4653 4837 1305
Size: 954 2394 184 2007
Fragments arranged by size:
2394 2007 954 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2660 2955 3632 4780 5025 1606
Size: 428 626 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 626 428 295 245
NaeI GCC'GGC
Cuts at: 2082 2082
Size: 5539
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NgoMIV G'CCGG_C
Cuts at: 2080 2080
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2383 2897 4442 1132
Size: 97 1154 514 1545 2229
Fragments arranged by size:
2229 1545 1154 514 97
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 4222
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PfoI T'CCnGG_A
Cuts at: 2241 2325 2241
Size: 84 5455
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PmlI CAC'GTG
Cuts at: 2596 2596
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PspOMI G'GGCC_C
Cuts at: 2095 2095
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2476 2891 830
Size: 1646 415 3478
Fragments arranged by size:
3478 1646 415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2660 2660
Size: 5539
SacI G_AGCT'C
Cuts at: 2838 2838
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SapI GCTCTTCn'nnn_
Cuts at: 2684 2684
Size: 5539
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SexAI A'CCwGG_T
Cuts at: 2409 2409
Size: 5539
SfcI C'TryA_G
Cuts at: 826 1045 2472 2887 4022 4703 4894 826
Size: 219 1427 415 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 415 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 2759 3643 4544 4806 820
Size: 197 926 546 270 884 901 262 1553
Fragments arranged by size:
1553 926 901 884 546 270 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 2255 1517
Size: 738 4801
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2373 2703 2978 513
Size: 552 939 207 162 330 275 3074
Fragments arranged by size:
3074 939 552 330 275 207 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 3566 3583 3736 3921 4366 325
Size: 1255 1161 825 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1161 825 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2255 2255
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI