Restriction map for
pFN21AB0985
(Circular) (Six-base) MAPSORT of: pFN21AB0985.seq Check: 3740 from: 1 to: 5635
pFN21AB0985 5635 bp
With 182 enzymes: SgfI *
March 28, 2012 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2683 2683
Size: 5635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AfeI AGC'GCT
Cuts at: 2109 2109
Size: 5635
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 5603
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaI G_GGCC'C
Cuts at: 2231 2231
Size: 5635
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2093 2944 3170 3322 5431 1493
Size: 600 851 226 152 2109 1697
Fragments arranged by size:
2109 1697 851 600 226 152
AscI GG'CGCG_CC
Cuts at: 2706 2706
Size: 5635
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2431 2671 2749 2870 2960 1870
Size: 73 190 298 240 78 121 90 4545
Fragments arranged by size:
4545 298 240 190 121 90 78 73
BamHI G'GATC_C
Cuts at: 2293 2435 2965 3332 2293
Size: 142 530 367 4596
Fragments arranged by size:
4596 530 367 142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2460 2466 2675 2774
Size: 325 217 627 447 226 6 209 99
Cuts at: 2774 2956 4411 618
Size: 182 1455 1842
Fragments arranged by size:
1842 1455 627 447 325 226 217 209
182 99 6
BanII G_rGCy'C
Cuts at: 1391 2231 2394 2478 2879 2954 1391
Size: 840 163 84 401 75 4072
Fragments arranged by size:
4072 840 401 163 84 75
BbeI G_GCGC'C
Cuts at: 2238 2464 2470 2238
Size: 226 6 5403
Fragments arranged by size:
5403 226 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3826 3860 1903
Size: 34 50 34 1805 34 3678
Fragments arranged by size:
3678 1805 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2036 2243
Size: 107 122 71 631 605 337 27 207
Cuts at: 2243 2772 4246 136
Size: 529 1474 1525
Fragments arranged by size:
1525 1474 631 605 529 337 207 122
107 71 27
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2231 3696 4852 1790
Size: 441 1465 1156 2573
Fragments arranged by size:
2573 1465 1156 441
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 2228 2276 2492 4295 1239
Size: 931 58 48 216 1803 2579
Fragments arranged by size:
2579 1803 931 216 58 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 4298 882
Size: 617 705 2094 2219
Fragments arranged by size:
2219 2094 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 2246 2361 2381
Size: 53 83 186 936 702 11 115 20
Cuts at: 2381 2461 2467 3442 3824 4459 275
Size: 80 6 975 382 635 1451
Fragments arranged by size:
1451 975 936 702 635 382 186 115
83 80 53 20 11 6
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 1178
Size: 30 165 30 1128 30 4252
Fragments arranged by size:
4252 1128 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 2294 2314 1186
Size: 1105 3 20 4507
Fragments arranged by size:
4507 1105 20 3
BseYI C'CCAG_C
Cuts at: 2712 4838 5334 2712
Size: 2126 496 3013
Fragments arranged by size:
3013 2126 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2771 1163
Size: 573 1035 4027
Fragments arranged by size:
4027 1035 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2370 3085 3846 4874 1540
Size: 275 186 369 715 761 1028 2301
Fragments arranged by size:
2301 1028 761 715 369 275 186
BsiHKAI G_wGCw'C
Cuts at: 3696 3781 4852 3696
Size: 85 1071 4479
Fragments arranged by size:
4479 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
BsmBI CGTCTCn'nnnn_
Cuts at: 2609 2609
Size: 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2394 2478 2879 2954 3696 3781
Size: 399 441 163 84 401 75 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 441 401 399 163 85
84 75
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2611 2683 2990 844
Size: 1767 72 307 3489
Fragments arranged by size:
3489 1767 307 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 3522 1394
Size: 429 497 1202 3507
Fragments arranged by size:
3507 1202 497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4130 4304 65
Size: 1183 2882 174 1396
Fragments arranged by size:
2882 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2237 2463 2777 4279 4466 1700
Size: 110 427 226 314 1502 187 2869
Fragments arranged by size:
2869 1502 427 314 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 2327 2348 2497 2499 2501 2706 1925
Size: 402 21 149 2 2 205 4854
Fragments arranged by size:
4854 402 205 149 21 2 2
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2293 2435 2965 3332 3717 3734 5186 5272
Size: 321 142 530 367 385 17 1452 86
Cuts at: 5272 5284 5630 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 530 385 367 346 321 142
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2306 2492 2814 513
Size: 552 1241 186 322 3334
Fragments arranged by size:
3334 1241 552 322 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3945 3965 3151
Size: 794 20 4821
Fragments arranged by size:
4821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4642 809
Size: 507 3326 1802
Fragments arranged by size:
3326 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2367 2817
Size: 54 1425 50 275 18 409 128 450
Cuts at: 2817 3082 3971 5600 8
Size: 265 889 1629 43
Fragments arranged by size:
1629 1425 889 450 409 275 265 128
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2367 3082 1537
Size: 275 555 715 4090
Fragments arranged by size:
4090 715 555 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2834 3565 1353
Size: 915 566 731 3423
Fragments arranged by size:
3423 915 731 566
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2171 2285 2827 4222 4596 4742
Size: 1064 138 582 114 542 1395 374 146
Cuts at: 4742 387
Size: 1280
Fragments arranged by size:
1395 1280 1064 582 542 374 146 138
114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2170 2228 2276 2492 3698 4295 5082
Size: 393 538 58 48 216 1206 597 787
Cuts at: 5082 5350 1239
Size: 268 1524
Fragments arranged by size:
1524 1206 787 597 538 393 268 216
58 48
EcoO109I rG'GnC_Cy
Cuts at: 2191 2424 2191
Size: 233 5402
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2111 2238 2464 2470 2584 2784 4782 1678
Size: 433 127 226 6 114 200 1998 2531
Fragments arranged by size:
2531 1998 433 226 200 127 114 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 4276 4308 4350
Size: 32 135 32 596 32 2076 32 42
Cuts at: 4350 4382 1373
Size: 32 2626
Fragments arranged by size:
2626 2076 596 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KasI G'GCGC_C
Cuts at: 2234 2460 2466 2234
Size: 226 6 5403
Fragments arranged by size:
5403 226 6
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2224 4749 4933 1305
Size: 919 2525 184 2007
Fragments arranged by size:
2525 2007 919 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3593 3952 4111 518
Size: 648 2427 359 159 2042
Fragments arranged by size:
2427 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2206 2308 2494 2527 2816 2854 3051 3728
Size: 600 102 186 33 289 38 197 677
Cuts at: 3728 4876 5121 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 289 245 197 186
102 38 33
NaeI GCC'GGC
Cuts at: 2239 2465 2239
Size: 226 5409
NarI GG'CG_CC
Cuts at: 2235 2461 2467 2235
Size: 226 6 5403
Fragments arranged by size:
5403 226 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5083
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2237 2463 2237
Size: 226 5409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2993 4538 1132
Size: 97 1764 1545 2229
Fragments arranged by size:
2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5635
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5635
PfoI T'CCnGG_A
Cuts at: 2070 2079 2070
Size: 9 5626
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 3704 3736 5217 5249 2531
Size: 32 1141 32 1481 32 2917
Fragments arranged by size:
2917 1481 1141 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2191 2424 2191
Size: 233 5402
PshAI GACnn'nnGTC
Cuts at: 1613 2621 1613
Size: 1008 4627
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PspOMI G'GGCC_C
Cuts at: 2227 2227
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2206 2527 2206
Size: 321 5314
SacII CC_GC'GG
Cuts at: 2309 2495 2817 2309
Size: 186 322 5127
Fragments arranged by size:
5127 322 186
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SanDI GG'GwC_CC
Cuts at: 2191 2191
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2983 4118 4799 4990 826
Size: 219 1938 1135 681 191 1471
Fragments arranged by size:
1938 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2236 2462 2468 2236
Size: 226 6 5403
Fragments arranged by size:
5403 226 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 2463 2777 1700
Size: 763 314 4558
Fragments arranged by size:
4558 763 314
SmaI CCC'GGG
Cuts at: 1872 2135 2433 2673 2872 2962 1872
Size: 263 298 240 199 90 4545
Fragments arranged by size:
4545 298 263 240 199 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2031 2535 2893 3074 513
Size: 552 966 504 358 181 3074
Fragments arranged by size:
3074 966 552 504 358 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5595 5627 5595
Size: 32 5603
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 1316
Size: 879 4756
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2149 2149
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2133 2431 2671 2870 2960 1870
Size: 263 298 240 199 90 4545
Fragments arranged by size:
4545 298 263 240 199 90
XmnI GAAnn'nnTTC
Cuts at: 2546 3764 2546
Size: 1218 4417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SapI SexAI SfiI SrfI SwaI