Restriction map for
pFN21AB0984
(Circular) (Six-base) MAPSORT of: pFN21AB0984.seq Check: 256 from: 1 to: 6061
pFN21AB0984 6061 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 4124 5508 5776 1632
Size: 1091 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1091 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 2367 4892 2367
Size: 2525 3536
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 2766 1166
Size: 1600 4461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2250 2505 2800 3804 5376 2250
Size: 255 295 1004 1572 2935
Fragments arranged by size:
2935 1572 1004 295 255
ApaI G_GGCC'C
Cuts at: 2826 2826
Size: 6061
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2008 2521 2625 2916 3370 3596 3748 5857
Size: 515 513 104 291 454 226 152 2109
Cuts at: 5857 1493
Size: 1697
Fragments arranged by size:
2109 1697 515 513 454 291 226 152
104
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3016 3386 1870
Size: 73 1073 370 4545
Fragments arranged by size:
4545 1073 370 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2267 2814 2826 3380 1391
Size: 876 547 12 554 4072
Fragments arranged by size:
4072 876 554 547 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5090
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 6061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2660 4672 136
Size: 107 122 71 631 605 988 2012 1525
Fragments arranged by size:
2012 1525 988 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2826 4122 5278 1790
Size: 310 726 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 726 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 3792 4750
Size: 416 53 83 399 24 1623 752 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1623 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2335 4721 1239
Size: 1096 2386 2579
Fragments arranged by size:
2579 2386 1096
Bpu10I CC'TnA_GC
Cuts at: 2261 2403 2261
Size: 142 5919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5051 5349 4186
Size: 865 298 4898
Fragments arranged by size:
4898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 3279 6055 3279
Size: 2776 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2367 4494 4892 5166 5313 5724 2367
Size: 2127 398 274 147 411 2704
Fragments arranged by size:
2704 2127 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2354 2384 1178
Size: 30 165 30 861 30 60 30 4855
Fragments arranged by size:
4855 861 165 60 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2711 3318 1186
Size: 1099 426 607 3929
Fragments arranged by size:
3929 1099 607 426
BseYI C'CCAG_C
Cuts at: 2793 5264 5760 2793
Size: 2471 496 3094
Fragments arranged by size:
3094 2471 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 2838 1163
Size: 573 1032 70 4386
Fragments arranged by size:
4386 1032 573 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2267 2351 2367 2814 4122 4207 5278 2162
Size: 105 84 16 447 1308 85 1071 2945
Fragments arranged by size:
2945 1308 1071 447 105 85 84 16
BsmI GAATG_Cn'
Cuts at: 2171 3572 3665 2171
Size: 1401 93 4567
Fragments arranged by size:
4567 1401 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2162 2267 2351 2367 2814 2826
Size: 399 310 62 105 84 16 447 12
Cuts at: 2826 3380 4122 4207 5278 1391
Size: 554 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 447 399 310 105
85 84 62 16 12
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 2575 2881 3950 5680 2575
Size: 306 1069 1730 2956
Fragments arranged by size:
2956 1730 1069 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3948 1394
Size: 429 2125 3507
Fragments arranged by size:
3507 2125 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2367 4705 4892 1700
Size: 110 557 2338 187 2869
Fragments arranged by size:
2869 2338 557 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6061
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 2080 1184
Size: 896 5165
BstYI r'GATC_y
Cuts at: 1972 3391 3758 4143 4160 5612 5698 5710 6056
Size: 1419 367 385 17 1452 86 12 346
Cuts at: 6056 1972
Size: 1977
Fragments arranged by size:
1977 1452 1419 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2967 3577 4371 4391 2967
Size: 610 794 20 4637
Fragments arranged by size:
4637 794 610 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4397 6026
Size: 54 1425 50 275 18 1678 889 1629
Cuts at: 6026 8
Size: 43
Fragments arranged by size:
1678 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3991 1353
Size: 2638 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2265 2812 2265
Size: 547 5514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2335 2723 4124 4721 5508 5776 1239
Size: 393 703 388 1401 597 787 268 1524
Fragments arranged by size:
1524 1401 787 703 597 393 388 268
EcoO109I rG'GnC_Cy
Cuts at: 2822 2822
Size: 6061
EcoRI G'AATT_C
Cuts at: 2008 3370 3748 2008
Size: 1362 378 4321
Fragments arranged by size:
4321 1362 378
EcoRV GAT'ATC
Cuts at: 2902 2902
Size: 6061
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2244 4702 4734 4776
Size: 32 135 32 640 32 2458 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 2458 640 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2178 3405 3651 669
Size: 988 281 240 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246 240
HindIII A'AGCT_T
Cuts at: 748 2420 2454 748
Size: 1672 34 4355
Fragments arranged by size:
4355 1672 34
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2766 2952 4019 4378 4537 518
Size: 648 1600 186 1067 359 159 2042
Fragments arranged by size:
2042 1600 1067 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 3477 4154 5302 5547 1606
Size: 1871 677 1148 245 2120
Fragments arranged by size:
2120 1871 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5509
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2791 2932 3419 4964 1132
Size: 97 1562 141 487 1545 2229
Fragments arranged by size:
2229 1562 1545 487 141 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2394 2505 1108
Size: 1286 111 4664
Fragments arranged by size:
4664 1286 111
PfoI T'CCnGG_A
Cuts at: 3053 3240 3053
Size: 187 5874
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspOMI G'GGCC_C
Cuts at: 2822 2822
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3413 830
Size: 2583 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SacI G_AGCT'C
Cuts at: 2267 2814 2267
Size: 547 5514
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 3409 4544 5225 5416 826
Size: 219 2364 1135 681 191 1471
Fragments arranged by size:
2364 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4165 5066 5328 820
Size: 197 926 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3026 3985 5
Size: 47 2974 959 2081
Fragments arranged by size:
2974 2081 959 47
StuI AGG'CCT
Cuts at: 1517 2113 3337 1517
Size: 596 1224 4241
Fragments arranged by size:
4241 1224 596
StyI C'CwwG_G
Cuts at: 513 1065 3500 513
Size: 552 2435 3074
Fragments arranged by size:
3074 2435 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3135 4088 4105 4258 4443 4888 325
Size: 1255 1555 953 17 153 185 445 1498
Fragments arranged by size:
1555 1498 1255 953 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4307 6019 96
Size: 274 80 33 51 1443 2330 1712 138
Fragments arranged by size:
2330 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2296 6021 6053 2264
Size: 32 3725 32 2272
Fragments arranged by size:
3725 2272 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 2382 1316
Size: 762 304 4995
Fragments arranged by size:
4995 762 304
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2066 2309 2628 4190 2066
Size: 243 319 1562 3937
Fragments arranged by size:
3937 1562 319 243
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI