Restriction map for
pFN21AB0983
(Circular) (Six-base) MAPSORT of: pFN21AB0983.seq Check: 7816 from: 1 to: 5425
pFN21AB0983 5425 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3235 278
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2768 1937
Size: 831 4594
Acc65I G'GTAC_C
Cuts at: 2287 2746 2287
Size: 459 4966
AclI AA'CG_TT
Cuts at: 1563 3552 3925 1563
Size: 1989 373 3063
Fragments arranged by size:
3063 1989 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3488 4872 5140 1632
Size: 1856 1384 268 1917
Fragments arranged by size:
1917 1856 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5228
AflIII A'CryG_T
Cuts at: 1203 4324 5301 1203
Size: 3121 977 1327
Fragments arranged by size:
3121 1327 977
AgeI A'CCGG_T
Cuts at: 4256 4256
Size: 5425
AhdI GACnn_n'nnGTC
Cuts at: 4154 4154
Size: 5425
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2048 1184
Size: 864 4561
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 5393
AlwNI CAG_nnn'CTG
Cuts at: 2393 2462 3168 4740 2393
Size: 69 706 1572 3078
Fragments arranged by size:
3078 1572 706 69
ApaI G_GGCC'C
Cuts at: 2606 2606
Size: 5425
ApaLI G'TGCA_C
Cuts at: 3482 4638 3482
Size: 1156 4269
ApoI r'AATT_y
Cuts at: 1493 2119 2734 2960 3112 5221 1493
Size: 626 615 226 152 2109 1697
Fragments arranged by size:
2109 1697 626 615 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3979 160
Size: 872 2947 1606
Fragments arranged by size:
2947 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
AvaI C'yCGr_G
Cuts at: 1870 1943 2283 2628 2750 1870
Size: 73 340 345 122 4545
Fragments arranged by size:
4545 345 340 122 73
BamHI G'GATC_C
Cuts at: 2755 3122 2755
Size: 367 5058
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2287 2746 4201 618
Size: 325 217 627 303 197 459 1455 1842
Fragments arranged by size:
1842 1455 627 459 325 303 217 197
BanII G_rGCy'C
Cuts at: 1391 2497 2606 2744 1391
Size: 1106 109 138 4072
Fragments arranged by size:
4072 1106 138 109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3616 3650 1903
Size: 34 1679 34 3678
Fragments arranged by size:
3678 1679 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2072 2248 3317 4527 2072
Size: 176 1069 1210 2970
Fragments arranged by size:
2970 1210 1069 176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4036 136
Size: 107 122 71 631 605 2364 1525
Fragments arranged by size:
2364 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5420 5420
Size: 5425
BlpI GC'TnA_GC
Cuts at: 2842 2842
Size: 5425
Bme1580I G_kGCm'C
Cuts at: 1790 2525 2606 3486 4642 1790
Size: 735 81 880 1156 2573
Fragments arranged by size:
2573 1156 880 735 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3156 4114 442
Size: 416 53 83 399 24 1739 958 1753
Fragments arranged by size:
1753 1739 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2688 2688
Size: 5425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2319 2328 2511 2544 4085 1239
Size: 1080 9 183 33 1541 2579
Fragments arranged by size:
2579 1541 1080 183 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3550 4415 4713 3550
Size: 865 298 4262
Fragments arranged by size:
4262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2584 2659 4088 882
Size: 617 1085 75 1429 2219
Fragments arranged by size:
2219 1429 1085 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 3216 493
Size: 711 2012 2702
Fragments arranged by size:
2702 2012 711
BsaBI GATnn'nnATC
Cuts at: 5419 5419
Size: 5425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2636 3232 3614 4249
Size: 53 83 186 936 1103 596 382 635
Cuts at: 4249 275
Size: 1451
Fragments arranged by size:
1451 1103 936 635 596 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3858 4256 4530 4677 5088 3858
Size: 398 274 147 411 4195
Fragments arranged by size:
4195 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2542 2572 1178
Size: 30 165 30 1139 30 4031
Fragments arranged by size:
4031 1139 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2355 2415 2565 1186
Size: 1169 60 150 4046
Fragments arranged by size:
4046 1169 150 60
BseYI C'CCAG_C
Cuts at: 2418 4628 5124 2418
Size: 2210 496 2719
Fragments arranged by size:
2719 2210 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4852
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2875 3636 4664 1540
Size: 275 186 874 761 1028 2301
Fragments arranged by size:
2301 1028 874 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 3571 4642 3486
Size: 85 1071 4269
Fragments arranged by size:
4269 1071 85
BsmI GAATG_Cn'
Cuts at: 2173 2712 2936 3029 2173
Size: 539 224 93 4569
Fragments arranged by size:
4569 539 224 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2497 2525 2606 2744 3486 3571 4642
Size: 399 707 28 81 138 742 85 1071
Cuts at: 4642 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 707 399 138 85 81
28
BspEI T'CCGG_A
Cuts at: 5088 5088
Size: 5425
BspHI T'CATG_A
Cuts at: 3314 5044 3314
Size: 1730 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2413 2780 844
Size: 1569 367 3489
Fragments arranged by size:
3489 1569 367
BsrBI CCG'CTC
Cuts at: 1394 1823 3312 1394
Size: 429 1489 3507
Fragments arranged by size:
3507 1489 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 3920 4094 65
Size: 1183 1029 1643 174 1396
Fragments arranged by size:
1643 1396 1183 1029 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4069 4256 1700
Size: 110 2259 187 2869
Fragments arranged by size:
2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5425
BssSI C'ACGA_G
Cuts at: 3485 4497 3485
Size: 1012 4413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2462 794
Size: 1668 3757
BstBI TT'CG_AA
Cuts at: 4229 4229
Size: 5425
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5425
BstXI CCAn_nnnn'nTGG
Cuts at: 2248 2248
Size: 5425
BstYI r'GATC_y
Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420
Size: 783 367 385 17 1452 86 12 346
Cuts at: 5420 1972
Size: 1977
Fragments arranged by size:
1977 1452 783 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2598 513
Size: 552 1533 3340
Fragments arranged by size:
3340 1533 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4686
Fragments arranged by size:
4686 683 56
BtsI GCAGTG_nn'
Cuts at: 2941 3735 3755 2941
Size: 794 20 4611
Fragments arranged by size:
4611 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5425
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5390
DraI TTT'AAA
Cuts at: 2729 2729
Size: 5425
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4432 809
Size: 507 3116 1802
Fragments arranged by size:
3116 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2872 3761
Size: 54 1425 50 275 18 244 798 889
Cuts at: 3761 5390 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 798 275 244 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2872 1537
Size: 275 1060 4090
Fragments arranged by size:
4090 1060 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2437 3355 1353
Size: 1084 918 3423
Fragments arranged by size:
3423 1084 918
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2517 4012 4386 4532 387
Size: 1064 138 928 1495 374 146 1280
Fragments arranged by size:
1495 1280 1064 928 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2319 2328 2511 2544 3488 4085 4872
Size: 393 687 9 183 33 944 597 787
Cuts at: 4872 5140 1239
Size: 268 1524
Fragments arranged by size:
1524 944 787 687 597 393 268 183
33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2397 2628 2676 2700 2397
Size: 231 48 24 5122
Fragments arranged by size:
5122 231 48 24
EcoO109I rG'GnC_Cy
Cuts at: 2312 2371 2390 2312
Size: 59 19 5347
Fragments arranged by size:
5347 59 19
EcoRI G'AATT_C
Cuts at: 2734 3112 2734
Size: 378 5047
FspI TGC'GCA
Cuts at: 3931 3931
Size: 5425
HaeII r_GCGC'y
Cuts at: 1678 4572 1678
Size: 2894 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373
Size: 32 135 32 2494 32 42 32 2626
Fragments arranged by size:
2626 2494 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2769 3015 669
Size: 988 281 831 246 3079
Fragments arranged by size:
3079 988 831 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5425
HpaI GTT'AAC
Cuts at: 3015 3015
Size: 5425
KpnI G_GTAC'C
Cuts at: 2291 2750 2291
Size: 459 4966
MfeI C'AATT_G
Cuts at: 2610 3024 2610
Size: 414 5011
MluI A'CGCG_T
Cuts at: 5301 5301
Size: 5425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4539 4723 1305
Size: 3234 184 2007
Fragments arranged by size:
3234 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3603
Fragments arranged by size:
3603 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2246 3383 3742 3901 518
Size: 648 1080 1137 359 159 2042
Fragments arranged by size:
2042 1137 1080 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2333 2351 2459 2841 3518 4666 4911 1606
Size: 727 18 108 382 677 1148 245 2120
Fragments arranged by size:
2120 1148 727 677 382 245 108 18
NcoI C'CATG_G
Cuts at: 513 1065 2598 513
Size: 552 1533 3340
Fragments arranged by size:
3340 1533 552
NdeI CA'TA_TG
Cuts at: 387 2115 387
Size: 1728 3697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5425
NotI GC'GGCC_GC
Cuts at: 2872 2872
Size: 5425
NspI r_CATG'y
Cuts at: 1132 1229 2650 2783 4328 1132
Size: 97 1421 133 1545 2229
Fragments arranged by size:
2229 1545 1421 133 97
PasI CC'CwG_GG
Cuts at: 1348 2315 1348
Size: 967 4458
PciI A'CATG_T
Cuts at: 4324 4324
Size: 5425
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 2612 1108
Size: 1189 315 3921
Fragments arranged by size:
3921 1189 315
PfoI T'CCnGG_A
Cuts at: 2656 2656
Size: 5425
PmeI GTTT'AAAC
Cuts at: 2729 2729
Size: 5425
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3494 3526 5007 5039 3494
Size: 32 1481 32 3880
Fragments arranged by size:
3880 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5425
PsiI TTA'TAA
Cuts at: 2995 2995
Size: 5425
PspOMI G'GGCC_C
Cuts at: 2602 2602
Size: 5425
PspXI vC'TCGA_Gb
Cuts at: 2628 2628
Size: 5425
PstI C_TGCA'G
Cuts at: 830 2037 2777 830
Size: 1207 740 3478
Fragments arranged by size:
3478 1207 740
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5425
PvuII CAG'CTG
Cuts at: 2333 2351 2459 2333
Size: 18 108 5299
Fragments arranged by size:
5299 108 18
SalI G'TCGA_C
Cuts at: 1936 2767 1936
Size: 831 4594
SbfI CC_TGCA'GG
Cuts at: 2777 2777
Size: 5425
ScaI AGT'ACT
Cuts at: 3673 5385 3673
Size: 1712 3713
SexAI A'CCwGG_T
Cuts at: 2581 2581
Size: 5425
SfcI C'TryA_G
Cuts at: 826 1045 2033 2047 2451 2773 3908 4589 4780
Size: 219 988 14 404 322 1135 681 191
Cuts at: 4780 826
Size: 1471
Fragments arranged by size:
1471 1135 988 681 404 322 219 191
14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5425
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5425
SmaI CCC'GGG
Cuts at: 1872 2752 1872
Size: 880 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2628 3529 4430 4692 820
Size: 197 926 685 901 901 262 1553
Fragments arranged by size:
1553 926 901 901 685 262 197
SnaBI TAC'GTA
Cuts at: 493 3216 493
Size: 2723 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5425
SphI G_CATG'C
Cuts at: 1132 2650 2783 1132
Size: 1518 133 3774
Fragments arranged by size:
3774 1518 133
SspI AAT'ATT
Cuts at: 5 52 3349 5
Size: 47 3297 2081
Fragments arranged by size:
3297 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5425
StyI C'CwwG_G
Cuts at: 513 1065 2598 2864 513
Size: 552 1533 266 3074
Fragments arranged by size:
3074 1533 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 3452 3469 3622 3807 4252 325
Size: 1255 1031 841 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1031 841 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3671 5383 96
Size: 274 80 33 51 1443 1694 1712 138
Fragments arranged by size:
1712 1694 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5385 5417 5385
Size: 32 5393
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5425
XbaI T'CTAG_A
Cuts at: 2761 2761
Size: 5425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2375 2375
Size: 5425
XhoI C'TCGA_G
Cuts at: 1943 2628 1943
Size: 685 4740
XmaI C'CCGG_G
Cuts at: 1870 2750 1870
Size: 880 4545
XmnI GAAnn'nnTTC
Cuts at: 3554 3554
Size: 5425
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3233 276
Size: 53 83 186 936 1699 2468
Fragments arranged by size:
2468 1699 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI