Restriction map for
pFN21AB0975
(Circular) (Six-base) MAPSORT of: pFN21AB0975.seq Check: 5090 from: 1 to: 5338
pFN21AB0975 5338 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2069 2509 2069
Size: 440 4898
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2210 3148 278
Size: 53 83 186 936 674 938 2468
Fragments arranged by size:
2468 938 936 674 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2681 1937
Size: 744 4594
Acc65I G'GTAC_C
Cuts at: 2659 2659
Size: 5338
AclI AA'CG_TT
Cuts at: 1563 3465 3838 1563
Size: 1902 373 3063
Fragments arranged by size:
3063 1902 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 3401 4785 5053 1632
Size: 932 837 1384 268 1917
Fragments arranged by size:
1917 1384 932 837 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5141
AflIII A'CryG_T
Cuts at: 1203 4237 5214 1203
Size: 3034 977 1327
Fragments arranged by size:
3034 1327 977
AgeI A'CCGG_T
Cuts at: 4169 4169
Size: 5338
AhdI GACnn_n'nnGTC
Cuts at: 4067 4067
Size: 5338
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 2405
Size: 32 5306
AlwNI CAG_nnn'CTG
Cuts at: 3081 4653 3081
Size: 1572 3766
ApaI G_GGCC'C
Cuts at: 2537 2537
Size: 5338
ApaLI G'TGCA_C
Cuts at: 2217 2297 3395 4551 2217
Size: 80 1098 1156 3004
Fragments arranged by size:
3004 1156 1098 80
ApoI r'AATT_y
Cuts at: 1493 2647 2873 3025 5134 1493
Size: 1154 226 152 2109 1697
Fragments arranged by size:
2109 1697 1154 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3892 160
Size: 872 2860 1606
Fragments arranged by size:
2860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2513 2663 1870
Size: 73 291 279 150 4545
Fragments arranged by size:
4545 291 279 150 73
AvrII C'CTAG_G
Cuts at: 2201 2201
Size: 5338
BamHI G'GATC_C
Cuts at: 2668 3035 2668
Size: 367 4971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2659 4114 618
Size: 325 217 627 302 570 1455 1842
Fragments arranged by size:
1842 1455 627 570 325 302 217
BanII G_rGCy'C
Cuts at: 1391 2537 2657 1391
Size: 1146 120 4072
Fragments arranged by size:
4072 1146 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2570 928
Size: 971 671 3696
Fragments arranged by size:
3696 971 671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3529 3563 1903
Size: 34 50 34 1508 34 3678
Fragments arranged by size:
3678 1508 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3230 4440 3230
Size: 1210 4128
BclI T'GATC_A
Cuts at: 1548 2265 1548
Size: 717 4621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3949 136
Size: 107 122 71 631 605 2277 1525
Fragments arranged by size:
2277 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5333 5333
Size: 5338
BlpI GC'TnA_GC
Cuts at: 2755 2755
Size: 5338
Bme1580I G_kGCm'C
Cuts at: 1790 2221 2301 2537 3399 4555 1790
Size: 431 80 236 862 1156 2573
Fragments arranged by size:
2573 1156 862 431 236 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3069 4027 442
Size: 416 53 83 399 24 1652 958 1753
Fragments arranged by size:
1753 1652 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 3998 1239
Size: 1199 1560 2579
Fragments arranged by size:
2579 1560 1199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3463 4328 4626 3463
Size: 865 298 4175
Fragments arranged by size:
4175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4001 882
Size: 617 2502 2219
Fragments arranged by size:
2502 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3129 493
Size: 711 1925 2702
Fragments arranged by size:
2702 1925 711
BsaBI GATnn'nnATC
Cuts at: 5332 5332
Size: 5338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2207 3145 3527 4162
Size: 53 83 186 936 674 938 382 635
Cuts at: 4162 275
Size: 1451
Fragments arranged by size:
1451 938 936 674 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3771 4169 4443 4590 5001 3771
Size: 398 274 147 411 4108
Fragments arranged by size:
4108 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 1178
Size: 30 165 30 1002 30 4081
Fragments arranged by size:
4081 1002 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2143 2539 1186
Size: 957 396 3985
Fragments arranged by size:
3985 957 396
BseYI C'CCAG_C
Cuts at: 4541 5037 4541
Size: 496 4842
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2103 2405 1163
Size: 573 367 302 4096
Fragments arranged by size:
4096 573 367 302
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2788 3549 4577 1540
Size: 275 186 787 761 1028 2301
Fragments arranged by size:
2301 1028 787 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2180 2221 2301 2531 3399 3484 4555 2180
Size: 41 80 230 868 85 1071 2963
Fragments arranged by size:
2963 1071 868 230 85 80 41
BsmI GAATG_Cn'
Cuts at: 2849 2942 2849
Size: 93 5245
BsmBI CGTCTCn'nnnn_
Cuts at: 2214 2441 2214
Size: 227 5111
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2180 2221 2301 2531 2537 2657 3399
Size: 399 390 41 80 230 6 120 742
Cuts at: 3399 3484 4555 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 230 120 85
80 41 6
BspEI T'CCGG_A
Cuts at: 5001 5001
Size: 5338
BspHI T'CATG_A
Cuts at: 3227 4957 3227
Size: 1730 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 2509 2693 844
Size: 1225 440 184 3489
Fragments arranged by size:
3489 1225 440 184
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 1394
Size: 429 1402 3507
Fragments arranged by size:
3507 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3833 4007 65
Size: 1183 2585 174 1396
Fragments arranged by size:
2585 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 3982 4169 1700
Size: 110 285 1887 187 2869
Fragments arranged by size:
2869 1887 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5338
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5338
BssSI C'ACGA_G
Cuts at: 3398 4410 3398
Size: 1012 4326
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5338
BstBI TT'CG_AA
Cuts at: 4142 4142
Size: 5338
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5338
BstYI r'GATC_y
Cuts at: 1972 2668 3035 3420 3437 4889 4975 4987 5333
Size: 696 367 385 17 1452 86 12 346
Cuts at: 5333 1972
Size: 1977
Fragments arranged by size:
1977 1452 696 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2319 2345 487
Size: 683 56 1093 26 3480
Fragments arranged by size:
3480 1093 683 56 26
BtsI GCAGTG_nn'
Cuts at: 2854 3648 3668 2854
Size: 794 20 4524
Fragments arranged by size:
4524 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5303
DraI TTT'AAA
Cuts at: 2642 2642
Size: 5338
DraIII CAC_nnn'GTG
Cuts at: 2441 2441
Size: 5338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4345 809
Size: 507 3029 1802
Fragments arranged by size:
3029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2785 3674 5303
Size: 54 1425 50 275 18 955 889 1629
Cuts at: 5303 8
Size: 43
Fragments arranged by size:
1629 1425 955 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2785 1537
Size: 275 973 4090
Fragments arranged by size:
4090 973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 3268 1353
Size: 1272 643 3423
Fragments arranged by size:
3423 1272 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2070 3925 4299 4445 387
Size: 1064 138 481 1855 374 146 1280
Fragments arranged by size:
1855 1280 1064 481 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2564 3401 3998 4785 5053 1239
Size: 393 806 126 837 597 787 268 1524
Fragments arranged by size:
1524 837 806 787 597 393 268 126
EcoNI CCTnn'n_nnAGG
Cuts at: 2200 2346 2200
Size: 146 5192
EcoO109I rG'GnC_Cy
Cuts at: 2122 2122
Size: 5338
EcoRI G'AATT_C
Cuts at: 2647 3025 2647
Size: 378 4960
FspI TGC'GCA
Cuts at: 3844 3844
Size: 5338
HaeII r_GCGC'y
Cuts at: 1678 4485 1678
Size: 2807 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3979 4011 4053 4085 1373
Size: 32 135 32 2407 32 42 32 2626
Fragments arranged by size:
2626 2407 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2682 2928 669
Size: 988 281 744 246 3079
Fragments arranged by size:
3079 988 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5338
HpaI GTT'AAC
Cuts at: 2928 2928
Size: 5338
KpnI G_GTAC'C
Cuts at: 2663 2663
Size: 5338
MfeI C'AATT_G
Cuts at: 2937 2937
Size: 5338
MluI A'CGCG_T
Cuts at: 5214 5214
Size: 5338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4452 4636 1305
Size: 3147 184 2007
Fragments arranged by size:
3147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3516
Fragments arranged by size:
3516 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 3296 3655 3814 518
Size: 648 1437 693 359 159 2042
Fragments arranged by size:
2042 1437 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2294 2754 3431 4579 4824 1606
Size: 688 460 677 1148 245 2120
Fragments arranged by size:
2120 1148 688 677 460 245
NaeI GCC'GGC
Cuts at: 2097 2097
Size: 5338
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4786
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5338
NgoMIV G'CCGG_C
Cuts at: 2095 2095
Size: 5338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5338
NotI GC'GGCC_GC
Cuts at: 2785 2785
Size: 5338
NspI r_CATG'y
Cuts at: 1132 1229 2696 4241 1132
Size: 97 1467 1545 2229
Fragments arranged by size:
2229 1545 1467 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5338
PciI A'CATG_T
Cuts at: 4237 4237
Size: 5338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2051 1108
Size: 943 4395
PmeI GTTT'AAAC
Cuts at: 2642 2642
Size: 5338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2437 3407 3439 4920 4952 2405
Size: 32 970 32 1481 32 2791
Fragments arranged by size:
2791 1481 970 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2122
Size: 5338
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5338
PsiI TTA'TAA
Cuts at: 2908 2908
Size: 5338
PspOMI G'GGCC_C
Cuts at: 2533 2533
Size: 5338
PstI C_TGCA'G
Cuts at: 830 2690 830
Size: 1860 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5338
SalI G'TCGA_C
Cuts at: 1936 2680 1936
Size: 744 4594
SanDI GG'GwC_CC
Cuts at: 2122 2122
Size: 5338
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 5338
SbfI CC_TGCA'GG
Cuts at: 2690 2690
Size: 5338
ScaI AGT'ACT
Cuts at: 3586 5298 3586
Size: 1712 3626
SfcI C'TryA_G
Cuts at: 826 1045 2317 2686 3821 4502 4693 826
Size: 219 1272 369 1135 681 191 1471
Fragments arranged by size:
1471 1272 1135 681 369 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5338
SgrAI Cr'CCGG_yG
Cuts at: 1700 2095 1700
Size: 395 4943
SmaI CCC'GGG
Cuts at: 1872 2515 2665 1872
Size: 643 150 4545
Fragments arranged by size:
4545 643 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3442 4343 4605 820
Size: 197 926 1499 901 262 1553
Fragments arranged by size:
1553 1499 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3129 493
Size: 2636 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5338
SphI G_CATG'C
Cuts at: 1132 2696 1132
Size: 1564 3774
SspI AAT'ATT
Cuts at: 5 52 3262 5
Size: 47 3210 2081
Fragments arranged by size:
3210 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5338
StyI C'CwwG_G
Cuts at: 513 1065 2050 2201 2777 513
Size: 552 985 151 576 3074
Fragments arranged by size:
3074 985 576 552 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3365 3382 3535 3720 4165 325
Size: 1255 1785 17 153 185 445 1498
Fragments arranged by size:
1785 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3584 5296 96
Size: 274 80 33 51 1443 1607 1712 138
Fragments arranged by size:
1712 1607 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5298 5330 5298
Size: 32 5306
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5338
XbaI T'CTAG_A
Cuts at: 2344 2674 2344
Size: 330 5008
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5338
XmaI C'CCGG_G
Cuts at: 1870 2513 2663 1870
Size: 643 150 4545
Fragments arranged by size:
4545 643 150
XmnI GAAnn'nnTTC
Cuts at: 3467 3467
Size: 5338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2208 3146 276
Size: 53 83 186 936 674 938 2468
Fragments arranged by size:
2468 938 936 674 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI XcmI