Restriction map for
pFN21AB0973
(Circular) (Six-base) MAPSORT of: pFN21AB0973.seq Check: 3554 from: 1 to: 5335
pFN21AB0973 5335 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 3398 4782 5050 1632
Size: 773 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 2660 1870
Size: 73 432 285 4545
Fragments arranged by size:
4545 432 285 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2550 2656 4111 618
Size: 325 217 627 763 106 1455 1842
Fragments arranged by size:
1842 1455 763 627 325 217 106
BanII G_rGCy'C
Cuts at: 1391 2132 2241 2654 1391
Size: 741 109 413 4072
Fragments arranged by size:
4072 741 413 109
BbeI G_GCGC'C
Cuts at: 2554 2554
Size: 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2028 2062 3526 3560 1903
Size: 34 50 34 7 34 1464 34 3678
Fragments arranged by size:
3678 1464 50 34 34 34 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2585 3946 136
Size: 107 122 71 631 605 913 1361 1525
Fragments arranged by size:
1525 1361 913 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2448 5330 2448
Size: 2882 2453
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2563 3396 4552 1790
Size: 773 833 1156 2573
Fragments arranged by size:
2573 1156 833 773
BmgBI CAC'GTC
Cuts at: 1318 2118 1318
Size: 800 4535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 3066 4024
Size: 416 53 83 399 24 610 1039 958
Cuts at: 4024 442
Size: 1753
Fragments arranged by size:
1753 1039 958 610 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3995 1239
Size: 2756 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 3460 4325 4623 2558
Size: 902 865 298 3270
Fragments arranged by size:
3270 902 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2175 2390 2436 3998 882
Size: 617 676 215 46 1562 2219
Fragments arranged by size:
2219 1562 676 617 215 46
BsaAI yAC'GTr
Cuts at: 493 1204 2602 3126 493
Size: 711 1398 524 2702
Fragments arranged by size:
2702 1398 711 524
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2551 3142 3524 4159
Size: 53 83 186 936 1018 591 382 635
Cuts at: 4159 275
Size: 1451
Fragments arranged by size:
1451 1018 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 1178
Size: 30 165 30 1032 30 4048
Fragments arranged by size:
4048 1032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 1186
Size: 925 4410
BseYI C'CCAG_C
Cuts at: 4538 5034 4538
Size: 496 4839
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4762
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3481 4552 3396
Size: 85 1071 4179
Fragments arranged by size:
4179 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
BsmBI CGTCTCn'nnnn_
Cuts at: 2108 2108
Size: 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2241 2563 2654 3396 3481 4552
Size: 399 342 109 322 91 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 342 322 109 91
85
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 2690 844
Size: 1634 212 3489
Fragments arranged by size:
3489 1634 212
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3979 4166 1700
Size: 110 2169 187 2869
Fragments arranged by size:
2869 2169 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 2104 1184
Size: 920 4415
BstYI r'GATC_y
Cuts at: 1972 2448 2665 3032 3417 3434 4886 4972 4984
Size: 476 217 367 385 17 1452 86 12
Cuts at: 4984 5330 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 476 385 367 346 217 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2019 2109 513
Size: 552 954 90 3739
Fragments arranged by size:
3739 954 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2369 2851 3645 3665 2369
Size: 482 794 20 4039
Fragments arranged by size:
4039 794 482 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2404 4342 809
Size: 507 1088 1938 1802
Fragments arranged by size:
1938 1802 1088 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2782 3671 5300
Size: 54 1425 50 275 18 952 889 1629
Cuts at: 5300 8
Size: 43
Fragments arranged by size:
1629 1425 952 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3922 4296 4442 387
Size: 1064 138 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2405 3398 3995 4782 5050 1239
Size: 393 773 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 773 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2299 2497 2299
Size: 198 5137
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 2060 3841 2060
Size: 1781 3554
HaeII r_GCGC'y
Cuts at: 1678 2554 4482 1678
Size: 876 1928 2531
Fragments arranged by size:
2531 1928 876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2433 2435 2465 2467 3976
Size: 32 135 32 861 2 30 2 1509
Cuts at: 3976 4008 4050 4082 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 861 135 42 32 32 32
32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2550 2550
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3652 3811 518
Size: 648 2127 359 159 2042
Fragments arranged by size:
2127 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2307 2751 3428 4576 4821 1606
Size: 440 261 444 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 444 440 261 245
NarI GG'CG_CC
Cuts at: 2551 2551
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 2542 387
Size: 2155 3180
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2065 2693 4238 1132
Size: 97 836 628 1545 2229
Fragments arranged by size:
2229 1545 836 628 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2497 2299
Size: 198 5137
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2585 2585
Size: 5335
SfoI GGC'GCC
Cuts at: 2552 2552
Size: 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2377 2662 1872
Size: 505 285 4545
Fragments arranged by size:
4545 505 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2573 3439 4340 4602 820
Size: 197 926 630 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 630 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2065 2693 1132
Size: 933 628 3774
Fragments arranged by size:
3774 933 628
SrfI GCCC'GGGC
Cuts at: 2377 2377
Size: 5335
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 2579 1517
Size: 1062 4273
StyI C'CwwG_G
Cuts at: 513 1065 2233 2774 513
Size: 552 1168 541 3074
Fragments arranged by size:
3074 1168 552 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2375 2660 1870
Size: 505 285 4545
Fragments arranged by size:
4545 505 285
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SwaI XcmI