Restriction map for
pFN21AB0967
(Circular) (Six-base) MAPSORT of: pFN21AB0967.seq Check: 3897 from: 1 to: 5323
pFN21AB0967 5323 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2644 2644
Size: 5323
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3386 4770 5038 1632
Size: 1754 1384 268 1917
Fragments arranged by size:
1917 1754 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2568 1184
Size: 1384 3939
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaLI G'TGCA_C
Cuts at: 3380 4536 3380
Size: 1156 4167
ApoI r'AATT_y
Cuts at: 1493 2632 2858 3010 5119 1493
Size: 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2065 2283 2648 1870
Size: 73 87 35 218 365 4545
Fragments arranged by size:
4545 365 218 87 73 35
AvrII C'CTAG_G
Cuts at: 2212 2212
Size: 5323
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2216 2241 2644 4099
Size: 325 217 627 379 50 25 403 1455
Cuts at: 4099 618
Size: 1842
Fragments arranged by size:
1842 1455 627 403 379 325 217 50
25
BanII G_rGCy'C
Cuts at: 1391 2031 2066 2096 2642 1391
Size: 640 35 30 546 4072
Fragments arranged by size:
4072 640 546 35 30
BbeI G_GCGC'C
Cuts at: 2170 2245 2170
Size: 75 5248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4352
BbvCI CC'TCA_GC
Cuts at: 2174 2445 2523 2174
Size: 271 78 4974
Fragments arranged by size:
4974 271 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5318 5318
Size: 5323
BlpI GC'TnA_GC
Cuts at: 2289 2740 2289
Size: 451 4872
Bme1580I G_kGCm'C
Cuts at: 1790 3384 4540 1790
Size: 1594 1156 2573
Fragments arranged by size:
2573 1594 1156
BmgBI CAC'GTC
Cuts at: 1318 2356 1318
Size: 1038 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 3983 1239
Size: 841 1903 2579
Fragments arranged by size:
2579 1903 841
Bpu10I CC'TnA_GC
Cuts at: 2174 2445 2523 2174
Size: 271 78 4974
Fragments arranged by size:
4974 271 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3448 4313 4611 2336
Size: 1112 865 298 3048
Fragments arranged by size:
3048 1112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3114 493
Size: 711 1910 2702
Fragments arranged by size:
2702 1910 711
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2167 2242 2414 3130
Size: 53 83 186 936 634 75 172 716
Cuts at: 3130 3512 4147 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 716 635 634 382 186 172
83 75 53
BsaWI w'CCGG_w
Cuts at: 3756 4154 4428 4575 4986 3756
Size: 398 274 147 411 4093
Fragments arranged by size:
4093 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5098
Fragments arranged by size:
5098 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5323
BseYI C'CCAG_C
Cuts at: 2293 2591 4526 5022 2293
Size: 298 1935 496 2594
Fragments arranged by size:
2594 1935 496 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2002 2528 1163
Size: 573 266 526 3958
Fragments arranged by size:
3958 573 526 266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3384 3469 4540 3384
Size: 85 1071 4167
Fragments arranged by size:
4167 1071 85
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2066 2096 2642 3384 3469 4540
Size: 399 241 35 30 546 742 85 1071
Cuts at: 4540 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 546 399 241 85 35
30
BspEI T'CCGG_A
Cuts at: 4986 4986
Size: 5323
BspHI T'CATG_A
Cuts at: 3212 4942 3212
Size: 1730 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 2550 2678 844
Size: 1575 131 128 3489
Fragments arranged by size:
3489 1575 131 128
BsrBI CCG'CTC
Cuts at: 1394 1823 3210 1394
Size: 429 1387 3507
Fragments arranged by size:
3507 1387 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2408 3818 3992 65
Size: 1183 1160 1410 174 1396
Fragments arranged by size:
1410 1396 1183 1160 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3967 4154 1700
Size: 110 2157 187 2869
Fragments arranged by size:
2869 2157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 3383 4395 3383
Size: 1012 4311
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5323
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5323
BstXI CCAn_nnnn'nTGG
Cuts at: 2271 2271
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318
Size: 681 367 385 17 1452 86 12 346
Cuts at: 5318 1972
Size: 1977
Fragments arranged by size:
1977 1452 681 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2023 2023
Size: 5323
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4584
Fragments arranged by size:
4584 683 56
BtsI GCAGTG_nn'
Cuts at: 2524 2839 3633 3653 2524
Size: 315 794 20 4194
Fragments arranged by size:
4194 794 315 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2770 3659
Size: 54 1425 50 275 18 312 628 889
Cuts at: 3659 5288 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 628 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3253 1353
Size: 1900 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 3386 3983 4770 5038 1239
Size: 393 448 1306 597 787 268 1524
Fragments arranged by size:
1524 1306 787 597 448 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2388 2388
Size: 5323
EcoO109I rG'GnC_Cy
Cuts at: 2055 2431 2527 2602 2055
Size: 376 96 75 4776
Fragments arranged by size:
4776 376 96 75
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 2170 2245 2583 4470 1678
Size: 492 75 338 1887 2531
Fragments arranged by size:
2531 1887 492 338 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 3964 3996 4038
Size: 32 135 32 710 32 1650 32 42
Cuts at: 4038 4070 1373
Size: 32 2626
Fragments arranged by size:
2626 1650 710 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KasI G'GCGC_C
Cuts at: 2166 2241 2166
Size: 75 5248
KpnI G_GTAC'C
Cuts at: 2648 2648
Size: 5323
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 3189
Fragments arranged by size:
3189 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3281 3640 3799 518
Size: 648 2115 359 159 2042
Fragments arranged by size:
2115 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2591 2739 3416 4564 4809 1606
Size: 985 148 677 1148 245 2120
Fragments arranged by size:
2120 1148 985 677 245 148
NarI GG'CG_CC
Cuts at: 2167 2242 2167
Size: 75 5248
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3832
Fragments arranged by size:
3832 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2681 4226 1132
Size: 97 1452 1545 2229
Fragments arranged by size:
2229 1545 1452 97
PasI CC'CwG_GG
Cuts at: 1348 2384 1348
Size: 1036 4287
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 4905 4937 3392
Size: 32 1481 32 3778
Fragments arranged by size:
3778 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2431 2431
Size: 5323
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2280 2376 2675 830
Size: 1450 96 299 3478
Fragments arranged by size:
3478 1450 299 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
PvuII CAG'CTG
Cuts at: 2591 2591
Size: 5323
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2376 2675 2376
Size: 299 5024
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 5323
SfcI C'TryA_G
Cuts at: 826 1045 2276 2372 2671 3806 4487 4678 826
Size: 219 1231 96 299 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 299 219 191 96
SfoI GGC'GCC
Cuts at: 2168 2243 2168
Size: 75 5248
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 3427 4328 4590 820
Size: 197 926 372 1112 901 262 1553
Fragments arranged by size:
1553 1112 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2212 2762 513
Size: 552 939 54 154 550 3074
Fragments arranged by size:
3074 939 552 550 154 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3367 3520 3705 4150 325
Size: 1255 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 2480 1316
Size: 1038 126 4159
Fragments arranged by size:
4159 1038 126
XbaI T'CTAG_A
Cuts at: 2659 2659
Size: 5323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 3452 3452
Size: 5323
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI