(Circular) (Six-base) MAPSORT of: pFN21AB0960.seq Check: 827 from: 1 to: 5980 pFN21AB0960 5980 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2973 3323 1937 Size: 1036 350 4594 Fragments arranged by size: 4594 1036 350 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2108 2708 4043 5427 5695 1632 Size: 416 60 600 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1335 600 416 268 60 AfeI AGC'GCT Cuts at: 2168 2168 Size: 5980 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 2857 4709 2857 Size: 1852 4128 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2187 1184 Size: 1003 4977 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3143 3175 3143 Size: 32 5948 AlwNI CAG_nnn'CTG Cuts at: 3723 5295 3723 Size: 1572 4408 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2650 3289 3515 3667 5776 1493 Size: 1157 639 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 639 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2584 2847 3147 3305 1870 Size: 73 208 433 263 300 158 4545 Fragments arranged by size: 4545 433 300 263 208 158 73 BamHI G'GATC_C Cuts at: 3310 3677 3310 Size: 367 5613 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2271 2372 3301 4756 Size: 325 217 627 253 231 101 929 1455 Cuts at: 4756 618 Size: 1842 Fragments arranged by size: 1842 1455 929 627 325 253 231 217 101 BanII G_rGCy'C Cuts at: 1391 2152 2954 3299 1391 Size: 761 802 345 4072 Fragments arranged by size: 4072 802 761 345 BbeI G_GCGC'C Cuts at: 2275 2275 Size: 5980 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 928 Size: 971 815 4194 Fragments arranged by size: 4194 971 815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2990 3872 5082 2990 Size: 882 1210 3888 Fragments arranged by size: 3888 1210 882 BclI T'GATC_A Cuts at: 1548 1548 Size: 5980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 2100 3397 2100 Size: 1297 4683 Bme1580I G_kGCm'C Cuts at: 1790 4041 5197 1790 Size: 2251 1156 2573 Fragments arranged by size: 2573 2251 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3200 3224 3278 4640 1239 Size: 1961 24 54 1362 2579 Fragments arranged by size: 2579 1961 1362 54 24 Bpu10I CC'TnA_GC Cuts at: 3105 3105 Size: 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3077 4105 4970 5268 3077 Size: 1028 865 298 3789 Fragments arranged by size: 3789 1028 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2010 2865 4643 882 Size: 617 511 855 1778 2219 Fragments arranged by size: 2219 1778 855 617 511 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2272 3787 4169 4804 Size: 53 83 186 936 739 1515 382 635 Cuts at: 4804 275 Size: 1451 Fragments arranged by size: 1515 1451 936 739 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 1178 Size: 30 165 30 654 30 5071 Fragments arranged by size: 5071 654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2321 2456 2459 2782 3086 3089 1186 Size: 946 189 135 3 323 304 3 4077 Fragments arranged by size: 4077 946 323 304 189 135 3 3 BseYI C'CCAG_C Cuts at: 2266 2897 5183 5679 2266 Size: 631 2286 496 2567 Fragments arranged by size: 2567 2286 631 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2643 2891 1163 Size: 573 907 248 4252 Fragments arranged by size: 4252 907 573 248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 2831 4041 4126 5197 2619 Size: 212 1210 85 1071 3402 Fragments arranged by size: 3402 1210 1071 212 85 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2619 2831 2954 3299 4041 4126 Size: 399 362 467 212 123 345 742 85 Cuts at: 4126 5197 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 467 399 362 345 212 123 85 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 3335 844 Size: 1427 1064 3489 Fragments arranged by size: 3489 1427 1064 BsrBI CCG'CTC Cuts at: 1394 1823 2104 3867 1394 Size: 429 281 1763 3507 Fragments arranged by size: 3507 1763 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 2234 2234 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2403 2780 3310 3677 4062 4079 5531 5617 Size: 431 377 530 367 385 17 1452 86 Cuts at: 5617 5629 5975 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 530 431 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2054 2054 Size: 5980 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 3496 4290 4310 3496 Size: 794 20 5166 Fragments arranged by size: 5166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5945 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 3427 4316 Size: 54 1425 50 275 18 564 1033 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1033 889 564 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2136 2151 2332 2426 2819 3910 1353 Size: 674 109 15 181 94 393 1091 3423 Fragments arranged by size: 3423 1091 674 393 181 109 94 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2028 4567 4941 5087 387 Size: 1064 138 439 2539 374 146 1280 Fragments arranged by size: 2539 1280 1064 439 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2108 2708 3200 3224 3278 4043 Size: 393 416 60 600 492 24 54 765 Cuts at: 4043 4640 5427 5695 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 765 600 597 492 416 393 268 60 54 24 EcoO109I rG'GnC_Cy Cuts at: 2231 2413 2729 2844 2231 Size: 182 316 115 5367 Fragments arranged by size: 5367 316 182 115 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2170 2275 5127 1678 Size: 492 105 2852 2531 Fragments arranged by size: 2852 2531 492 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 2843 2875 3072 Size: 32 135 32 1006 32 233 32 197 Cuts at: 3072 3104 3173 3205 4621 4653 4695 4727 1373 Size: 32 69 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 1006 233 197 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KasI G'GCGC_C Cuts at: 2271 2271 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 5094 5278 1305 Size: 888 2901 184 2007 Fragments arranged by size: 2901 2007 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 10 Size: 54 1425 343 564 3594 Fragments arranged by size: 3594 1425 564 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3938 4297 4456 518 Size: 648 2772 359 159 2042 Fragments arranged by size: 2772 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 3396 4073 5221 5466 1606 Size: 522 1268 677 1148 245 2120 Fragments arranged by size: 2120 1268 1148 677 522 245 NarI GG'CG_CC Cuts at: 2272 2272 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2816 2990 3104 3338 4883 1132 Size: 97 1587 174 114 234 1545 2229 Fragments arranged by size: 2229 1587 1545 234 174 114 97 PasI CC'CwG_GG Cuts at: 1348 2869 1348 Size: 1521 4459 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5980 PfoI T'CCnGG_A Cuts at: 2406 2992 2406 Size: 586 5394 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2231 2413 2729 2844 2231 Size: 182 316 115 5367 Fragments arranged by size: 5367 316 182 115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspXI vC'TCGA_Gb Cuts at: 2584 2584 Size: 5980 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2581 2613 2581 Size: 32 5948 PstI C_TGCA'G Cuts at: 830 3019 3332 830 Size: 2189 313 3478 Fragments arranged by size: 3478 2189 313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SanDI GG'GwC_CC Cuts at: 2844 2844 Size: 5980 SapI GCTCTTCn'nnn_ Cuts at: 2027 2332 2819 2027 Size: 305 487 5188 Fragments arranged by size: 5188 487 305 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 3015 3328 4463 5144 5335 826 Size: 219 1970 313 1135 681 191 1471 Fragments arranged by size: 1970 1471 1135 681 313 219 191 SfoI GGC'GCC Cuts at: 2273 2273 Size: 5980 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 2153 2849 3149 3307 1872 Size: 281 696 300 158 4545 Fragments arranged by size: 4545 696 300 281 158 SmlI C'TyrA_G Cuts at: 820 1017 1943 2584 3092 4084 4985 5247 820 Size: 197 926 641 508 992 901 262 1553 Fragments arranged by size: 1553 992 926 901 641 508 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3104 3338 1132 Size: 1972 234 3774 Fragments arranged by size: 3774 1972 234 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 3419 513 Size: 552 939 1415 3074 Fragments arranged by size: 3074 1415 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4007 4024 4177 4362 4807 325 Size: 1255 2427 17 153 185 445 1498 Fragments arranged by size: 2427 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 5940 5972 2057 Size: 32 3851 32 2065 Fragments arranged by size: 3851 2065 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2234 2234 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 2584 1943 Size: 641 5339 XmaI C'CCGG_G Cuts at: 1870 2151 2847 3147 3305 1870 Size: 281 696 300 158 4545 Fragments arranged by size: 4545 696 300 281 158 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PvuII RsrII SacI SacII SexAI SfiI SrfI SwaI