Restriction map for
pFN21AB0960
(Circular) (Six-base) MAPSORT of: pFN21AB0960.seq Check: 827 from: 1 to: 5980
pFN21AB0960 5980 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2973 3323 1937
Size: 1036 350 4594
Fragments arranged by size:
4594 1036 350
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2108 2708 4043 5427 5695 1632
Size: 416 60 600 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 600 416 268 60
AfeI AGC'GCT
Cuts at: 2168 2168
Size: 5980
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 2857 4709 2857
Size: 1852 4128
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2187 1184
Size: 1003 4977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 5948
AlwNI CAG_nnn'CTG
Cuts at: 3723 5295 3723
Size: 1572 4408
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 2650 3289 3515 3667 5776 1493
Size: 1157 639 226 152 2109 1697
Fragments arranged by size:
2109 1697 1157 639 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2151 2584 2847 3147 3305 1870
Size: 73 208 433 263 300 158 4545
Fragments arranged by size:
4545 433 300 263 208 158 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2271 2372 3301 4756
Size: 325 217 627 253 231 101 929 1455
Cuts at: 4756 618
Size: 1842
Fragments arranged by size:
1842 1455 929 627 325 253 231 217
101
BanII G_rGCy'C
Cuts at: 1391 2152 2954 3299 1391
Size: 761 802 345 4072
Fragments arranged by size:
4072 802 761 345
BbeI G_GCGC'C
Cuts at: 2275 2275
Size: 5980
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 928
Size: 971 815 4194
Fragments arranged by size:
4194 971 815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2990 3872 5082 2990
Size: 882 1210 3888
Fragments arranged by size:
3888 1210 882
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 2100 3397 2100
Size: 1297 4683
Bme1580I G_kGCm'C
Cuts at: 1790 4041 5197 1790
Size: 2251 1156 2573
Fragments arranged by size:
2573 2251 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3200 3224 3278 4640 1239
Size: 1961 24 54 1362 2579
Fragments arranged by size:
2579 1961 1362 54 24
Bpu10I CC'TnA_GC
Cuts at: 3105 3105
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3077 4105 4970 5268 3077
Size: 1028 865 298 3789
Fragments arranged by size:
3789 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2010 2865 4643 882
Size: 617 511 855 1778 2219
Fragments arranged by size:
2219 1778 855 617 511
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2272 3787 4169 4804
Size: 53 83 186 936 739 1515 382 635
Cuts at: 4804 275
Size: 1451
Fragments arranged by size:
1515 1451 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2057 2087 1178
Size: 30 165 30 654 30 5071
Fragments arranged by size:
5071 654 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2321 2456 2459 2782 3086 3089 1186
Size: 946 189 135 3 323 304 3 4077
Fragments arranged by size:
4077 946 323 304 189 135 3 3
BseYI C'CCAG_C
Cuts at: 2266 2897 5183 5679 2266
Size: 631 2286 496 2567
Fragments arranged by size:
2567 2286 631 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2643 2891 1163
Size: 573 907 248 4252
Fragments arranged by size:
4252 907 573 248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 2831 4041 4126 5197 2619
Size: 212 1210 85 1071 3402
Fragments arranged by size:
3402 1210 1071 212 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2619 2831 2954 3299 4041 4126
Size: 399 362 467 212 123 345 742 85
Cuts at: 4126 5197 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 362 345 212
123 85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2271 3335 844
Size: 1427 1064 3489
Fragments arranged by size:
3489 1427 1064
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 3867 1394
Size: 429 281 1763 3507
Fragments arranged by size:
3507 1763 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstXI CCAn_nnnn'nTGG
Cuts at: 2234 2234
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2403 2780 3310 3677 4062 4079 5531 5617
Size: 431 377 530 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 530 431 385 377 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2054 2054
Size: 5980
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 3496 4290 4310 3496
Size: 794 20 5166
Fragments arranged by size:
5166 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 3427 4316
Size: 54 1425 50 275 18 564 1033 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1033 889 564 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2136 2151 2332 2426 2819 3910 1353
Size: 674 109 15 181 94 393 1091 3423
Fragments arranged by size:
3423 1091 674 393 181 109 94 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2028 4567 4941 5087 387
Size: 1064 138 439 2539 374 146 1280
Fragments arranged by size:
2539 1280 1064 439 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2108 2708 3200 3224 3278 4043
Size: 393 416 60 600 492 24 54 765
Cuts at: 4043 4640 5427 5695 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 765 600 597 492 416 393
268 60 54 24
EcoO109I rG'GnC_Cy
Cuts at: 2231 2413 2729 2844 2231
Size: 182 316 115 5367
Fragments arranged by size:
5367 316 182 115
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2170 2275 5127 1678
Size: 492 105 2852 2531
Fragments arranged by size:
2852 2531 492 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2578 2610 2843 2875 3072
Size: 32 135 32 1006 32 233 32 197
Cuts at: 3072 3104 3173 3205 4621 4653 4695 4727 1373
Size: 32 69 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 1006 233 197 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KasI G'GCGC_C
Cuts at: 2271 2271
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 5094 5278 1305
Size: 888 2901 184 2007
Fragments arranged by size:
2901 2007 888 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 10
Size: 54 1425 343 564 3594
Fragments arranged by size:
3594 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4297 4456 518
Size: 648 2772 359 159 2042
Fragments arranged by size:
2772 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 3396 4073 5221 5466 1606
Size: 522 1268 677 1148 245 2120
Fragments arranged by size:
2120 1268 1148 677 522 245
NarI GG'CG_CC
Cuts at: 2272 2272
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2816 2990 3104 3338 4883 1132
Size: 97 1587 174 114 234 1545 2229
Fragments arranged by size:
2229 1587 1545 234 174 114 97
PasI CC'CwG_GG
Cuts at: 1348 2869 1348
Size: 1521 4459
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PfoI T'CCnGG_A
Cuts at: 2406 2992 2406
Size: 586 5394
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2231 2413 2729 2844 2231
Size: 182 316 115 5367
Fragments arranged by size:
5367 316 182 115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspXI vC'TCGA_Gb
Cuts at: 2584 2584
Size: 5980
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2581 2613 2581
Size: 32 5948
PstI C_TGCA'G
Cuts at: 830 3019 3332 830
Size: 2189 313 3478
Fragments arranged by size:
3478 2189 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SanDI GG'GwC_CC
Cuts at: 2844 2844
Size: 5980
SapI GCTCTTCn'nnn_
Cuts at: 2027 2332 2819 2027
Size: 305 487 5188
Fragments arranged by size:
5188 487 305
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 3015 3328 4463 5144 5335 826
Size: 219 1970 313 1135 681 191 1471
Fragments arranged by size:
1970 1471 1135 681 313 219 191
SfoI GGC'GCC
Cuts at: 2273 2273
Size: 5980
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2153 2849 3149 3307 1872
Size: 281 696 300 158 4545
Fragments arranged by size:
4545 696 300 281 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2584 3092 4084 4985 5247 820
Size: 197 926 641 508 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 641 508 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3104 3338 1132
Size: 1972 234 3774
Fragments arranged by size:
3774 1972 234
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 3419 513
Size: 552 939 1415 3074
Fragments arranged by size:
3074 1415 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 5940 5972 2057
Size: 32 3851 32 2065
Fragments arranged by size:
3851 2065 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2234 2234
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 2584 1943
Size: 641 5339
XmaI C'CCGG_G
Cuts at: 1870 2151 2847 3147 3305 1870
Size: 281 696 300 158 4545
Fragments arranged by size:
4545 696 300 281 158
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PvuII RsrII SacI SacII SexAI SfiI SrfI
SwaI