Restriction map for
pFN21AB0956
(Circular) (Six-base) MAPSORT of: pFN21AB0956.seq Check: 1957 from: 1 to: 5959
pFN21AB0956 5959 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2441 2918 4022 5406 5674 1632
Size: 809 477 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1104 809 477 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2025 4858 5835 1203
Size: 822 2833 977 1327
Fragments arranged by size:
2833 1327 977 822
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2955 1166
Size: 1789 4170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3027 1184
Size: 1843 4116
AlwNI CAG_nnn'CTG
Cuts at: 2263 2287 2387 2407 2891 3158 3702 5274 2263
Size: 24 100 20 484 267 544 1572 2948
Fragments arranged by size:
2948 1572 544 484 267 100 24 20
ApaLI G'TGCA_C
Cuts at: 2305 4016 5172 2305
Size: 1711 1156 3092
Fragments arranged by size:
3092 1711 1156
ApoI r'AATT_y
Cuts at: 1493 2639 3042 3268 3494 3646 5755 1493
Size: 1146 403 226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1146 403 226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4570 136
Size: 107 122 71 631 605 337 2561 1525
Fragments arranged by size:
2561 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2233 2309 2414 2964 4020 5176 1790
Size: 443 76 105 550 1056 1156 2573
Fragments arranged by size:
2573 1156 1056 550 443 105 76
BmgBI CAC'GTC
Cuts at: 1318 2026 1318
Size: 708 5251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 3690 4648
Size: 416 53 83 399 24 1155 1118 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1155 1118 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2703 2837 2913 3038 4619 1239
Size: 1464 134 76 125 1581 2579
Fragments arranged by size:
2579 1581 1464 134 125 76
Bpu10I CC'TnA_GC
Cuts at: 2786 2786
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2071 2135 2739 2966 4622 882
Size: 617 572 64 604 227 1656 2219
Fragments arranged by size:
2219 1656 617 604 572 227 64
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2850 3766 4148 4783
Size: 53 83 186 936 1317 916 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1451 1317 936 916 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2130 2730 4392 4790 5064 5211 5622 2130
Size: 600 1662 398 274 147 411 2467
Fragments arranged by size:
2467 1662 600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 1178
Size: 30 165 30 612 30 5092
Fragments arranged by size:
5092 612 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2485 2621 3212 3215 3218 3221 1186
Size: 1075 224 136 591 3 3 3 3924
Fragments arranged by size:
3924 1075 591 224 136 3 3 3
BseYI C'CCAG_C
Cuts at: 3235 5162 5658 3235
Size: 1927 496 3536
Fragments arranged by size:
3536 1927 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2157 2385 1163
Size: 573 421 228 4737
Fragments arranged by size:
4737 573 421 228
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2309 4020 4105 5176 2309
Size: 1711 85 1071 3092
Fragments arranged by size:
3092 1711 1071 85
BsmI GAATG_Cn'
Cuts at: 2194 2257 3470 3563 2194
Size: 63 1213 93 4590
Fragments arranged by size:
4590 1213 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2309 2414 2964 3278 4020 4105
Size: 399 443 76 105 550 314 742 85
Cuts at: 4105 5176 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 550 443 399 314 105
85 76
BspEI T'CCGG_A
Cuts at: 2130 5622 2130
Size: 3492 2467
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 4454 4628 65
Size: 1183 1093 2113 174 1396
Fragments arranged by size:
2113 1396 1183 1093 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2058 1925
Size: 133 5826
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 3148 3148
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2915 3102 3289 3656 4041 4058 5510 5596
Size: 943 187 187 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 943 385 367 346 187 187
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2225 2829 3107 2225
Size: 604 278 5077
Fragments arranged by size:
5077 604 278
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 513
Size: 552 939 66 4402
Fragments arranged by size:
4402 939 552 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 2218 2368 3009 3475 4269 4289 2218
Size: 150 641 466 794 20 3888
Fragments arranged by size:
3888 794 641 466 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2761 3406
Size: 54 1425 50 275 18 696 235 645
Cuts at: 3406 4295 5924 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 696 645 275 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2598 2678 3177 3183 3189 3889 1353
Size: 1245 80 499 6 6 700 3423
Fragments arranged by size:
3423 1245 700 499 80 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2703 2837 2913 2918 3038 4022
Size: 393 809 262 134 76 5 120 984
Cuts at: 4022 4619 5406 5674 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 984 809 787 597 393 268 262
134 120 76 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2690 3152 2690
Size: 462 5497
EcoO109I rG'GnC_Cy
Cuts at: 2404 2417 2683 2404
Size: 13 266 5680
Fragments arranged by size:
5680 266 13
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 2315 4465 2315
Size: 2150 3809
HaeII r_GCGC'y
Cuts at: 1678 2097 5106 1678
Size: 419 3009 2531
Fragments arranged by size:
3009 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2907 2939 4600
Size: 32 135 32 443 32 860 32 1661
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1661 860 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2453 748
Size: 1705 4254
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2540 5073 5257 1305
Size: 1235 2533 184 2007
Fragments arranged by size:
2533 2007 1235 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 2763 10
Size: 54 1425 343 696 235 3206
Fragments arranged by size:
3206 1425 696 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2510 2805 2955 3917 4276 4435 518
Size: 648 1344 295 150 962 359 159 2042
Fragments arranged by size:
2042 1344 962 648 359 295 159 150
MspA1I CmG'CkG
Cuts at: 1606 3073 3375 4052 5200 5445 1606
Size: 1467 302 677 1148 245 2120
Fragments arranged by size:
2120 1467 1148 677 302 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4468
Fragments arranged by size:
4468 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3008 3317 4862 1132
Size: 97 1779 309 1545 2229
Fragments arranged by size:
2229 1779 1545 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2404
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 3066 1613
Size: 1453 4506
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2363 3311 830
Size: 1533 948 3478
Fragments arranged by size:
3478 1533 948
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SexAI A'CCwGG_T
Cuts at: 2400 2400
Size: 5959
SfcI C'TryA_G
Cuts at: 826 1045 2359 3307 4442 5123 5314 826
Size: 219 1314 948 1135 681 191 1471
Fragments arranged by size:
1471 1314 1135 948 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 2097 2764 3398 513
Size: 552 939 93 667 634 3074
Fragments arranged by size:
3074 939 667 634 552 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 3986 4003 4156 4341 4786 325
Size: 1255 730 1676 17 153 185 445 1498
Fragments arranged by size:
1676 1498 1255 730 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 4205 5917
Size: 274 80 33 51 1443 378 1850 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1850 1712 1443 378 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 5919 5951 2112
Size: 32 3775 32 2120
Fragments arranged by size:
3775 2120 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI