Restriction map for
pFN21AB0950
(Circular) (Six-base) MAPSORT of: pFN21AB0950.seq Check: 2828 from: 1 to: 5926
pFN21AB0950 5926 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 5373 5641 1632
Size: 2357 1384 268 1917
Fragments arranged by size:
2357 1917 1384 268
AfeI AGC'GCT
Cuts at: 2298 2298
Size: 5926
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 3039 4757 3039
Size: 1718 4208
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 2879 3251 1870
Size: 73 582 354 372 4545
Fragments arranged by size:
4545 582 372 354 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2313 2655 2765 3247 4702
Size: 325 217 627 526 342 110 482 1455
Cuts at: 4702 618
Size: 1842
Fragments arranged by size:
1842 1455 627 526 482 342 325 217
110
BanII G_rGCy'C
Cuts at: 1391 2076 2880 3245 1391
Size: 685 804 365 4072
Fragments arranged by size:
4072 804 685 365
BbeI G_GCGC'C
Cuts at: 2659 2659
Size: 5926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 928
Size: 971 156 4799
Fragments arranged by size:
4799 971 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2860 2894 4117 4151 1903
Size: 34 923 34 1223 34 3678
Fragments arranged by size:
3678 1223 923 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3818 5028 3818
Size: 1210 4716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2774 2855 4537
Size: 107 122 71 631 605 1102 81 1682
Cuts at: 4537 136
Size: 1525
Fragments arranged by size:
1682 1525 1102 631 605 122 107 81
71
BglII A'GATC_T
Cuts at: 2988 5921 2988
Size: 2933 2993
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2556 2770 3987 5143 1790
Size: 766 214 1217 1156 2573
Fragments arranged by size:
2573 1217 1156 766 214
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2618 2965 3657
Size: 416 53 83 399 24 1201 347 692
Cuts at: 3657 4615 442
Size: 958 1753
Fragments arranged by size:
1753 1201 958 692 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3173 1056
Size: 2117 3809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4586 1239
Size: 3347 2579
Bpu10I CC'TnA_GC
Cuts at: 2085 2085
Size: 5926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4051 4916 5214 4051
Size: 865 298 4763
Fragments arranged by size:
4763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4589 882
Size: 617 3090 2219
Fragments arranged by size:
3090 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2887 3717 493
Size: 711 1683 830 2702
Fragments arranged by size:
2702 1683 830 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2656 2787 3733 4115
Size: 53 83 186 936 1123 131 946 382
Cuts at: 4115 4750 275
Size: 635 1451
Fragments arranged by size:
1451 1123 946 936 635 382 186 131
83 53
BsaWI w'CCGG_w
Cuts at: 3039 4359 4757 5031 5178 5589 3039
Size: 1320 398 274 147 411 3376
Fragments arranged by size:
3376 1320 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 1186
Size: 1333 4593
BseYI C'CCAG_C
Cuts at: 2396 2492 2815 5129 5625 2396
Size: 96 323 2314 496 2697
Fragments arranged by size:
2697 2314 496 323 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2273 2617 3376 4137 5165 1540
Size: 275 186 272 344 759 761 1028 2301
Fragments arranged by size:
2301 1028 761 759 344 275 272 186
BsiHKAI G_wGCw'C
Cuts at: 3987 4072 5143 3987
Size: 85 1071 4770
Fragments arranged by size:
4770 1071 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 2658
Size: 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2556 2770 2880 3245 3987 4072
Size: 399 286 480 214 110 365 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 365 286 214
110 85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 3281 844
Size: 1286 1151 3489
Fragments arranged by size:
3489 1286 1151
BsrBI CCG'CTC
Cuts at: 1394 1823 2440 3142 3813 1394
Size: 429 617 702 671 3507
Fragments arranged by size:
3507 702 671 617 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3003 3039 3063 4570 4757 1700
Size: 110 1193 36 24 1507 187 2869
Fragments arranged by size:
2869 1507 1193 187 110 36 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3077 3986 4998 3077
Size: 909 1012 4005
Fragments arranged by size:
4005 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2457 794
Size: 1663 4263
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 2302 1184
Size: 1118 4808
BstXI CCAn_nnnn'nTGG
Cuts at: 2851 2915 2851
Size: 64 5862
BstYI r'GATC_y
Cuts at: 1972 2988 3256 3623 4008 4025 5477 5563 5575
Size: 1016 268 367 385 17 1452 86 12
Cuts at: 5575 5921 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 385 367 346 268 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2346 2997 2346
Size: 651 5275
BtgI C'CryG_G
Cuts at: 513 1065 2004 2710 2850 2908 3009 513
Size: 552 939 706 140 58 101 3430
Fragments arranged by size:
3430 939 706 552 140 101 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2928 487
Size: 683 56 1702 3485
Fragments arranged by size:
3485 1702 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2476 2482 2800 3442 4236 4256 2217
Size: 259 6 318 642 794 20 3887
Fragments arranged by size:
3887 794 642 318 259 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2632 2667 529
Size: 35 2068 35 3788
Fragments arranged by size:
3788 2068 35 35
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DraIII CAC_nnn'GTG
Cuts at: 3082 3082
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4933 809
Size: 507 3617 1802
Fragments arranged by size:
3617 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2092 2270 2625
Size: 54 1425 50 275 18 262 178 355
Cuts at: 2625 3373 4262 5891 8
Size: 748 889 1629 43
Fragments arranged by size:
1629 1425 889 748 355 275 262 178
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2270 3373 1537
Size: 275 458 1103 4090
Fragments arranged by size:
4090 1103 458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 3856 1353
Size: 882 1621 3423
Fragments arranged by size:
3423 1621 882
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2786 3082 4513 4887 5033 387
Size: 1064 138 1197 296 1431 374 146 1280
Fragments arranged by size:
1431 1280 1197 1064 374 296 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3989 4586 5373 5641 1239
Size: 393 2357 597 787 268 1524
Fragments arranged by size:
2357 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2413 2036
Size: 377 5549
EcoO109I rG'GnC_Cy
Cuts at: 2190 2350 2638 2190
Size: 160 288 5478
Fragments arranged by size:
5478 288 160
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 5926
FseI GG_CCGG'CC
Cuts at: 3007 3007
Size: 5926
FspI TGC'GCA
Cuts at: 2806 4432 2806
Size: 1626 4300
HaeII r_GCGC'y
Cuts at: 1678 2292 2300 2513 2533 2659 2923 5073 1678
Size: 614 8 213 20 126 264 2150 2531
Fragments arranged by size:
2531 2150 614 264 213 126 20 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4567 4599 4641 4673 1373
Size: 32 135 32 2995 32 42 32 2626
Fragments arranged by size:
2995 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2899 3270 3516 669
Size: 988 281 961 371 246 3079
Fragments arranged by size:
3079 988 961 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2655 2655
Size: 5926
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2913 3884 4243 4402 518
Size: 648 1747 971 359 159 2042
Fragments arranged by size:
2042 1747 971 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2275 2463 3342 4019 5167 5412 1606
Size: 669 188 879 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 669 245 188
NaeI GCC'GGC
Cuts at: 3005 3005
Size: 5926
NarI GG'CG_CC
Cuts at: 2656 2656
Size: 5926
NcoI C'CATG_G
Cuts at: 513 1065 2004 2850 513
Size: 552 939 846 3589
Fragments arranged by size:
3589 939 846 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 3003 3003
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 3169 1052
Size: 2117 3809
NotI GC'GGCC_GC
Cuts at: 2270 3373 2270
Size: 1103 4823
NspI r_CATG'y
Cuts at: 1132 1229 2563 3284 4829 1132
Size: 97 1334 721 1545 2229
Fragments arranged by size:
2229 1545 1334 721 97
PasI CC'CwG_GG
Cuts at: 1348 2332 2409 1348
Size: 984 77 4865
Fragments arranged by size:
4865 984 77
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 1108
Size: 1215 4711
PfoI T'CCnGG_A
Cuts at: 2155 2155
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2190 2190
Size: 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2280 2296 2462 2468 2988 3278 830
Size: 1450 16 166 6 520 290 3478
Fragments arranged by size:
3478 1450 520 290 166 16 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2463 2463
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SanDI GG'GwC_CC
Cuts at: 2190 2190
Size: 5926
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SexAI A'CCwGG_T
Cuts at: 3101 3101
Size: 5926
SfcI C'TryA_G
Cuts at: 826 1045 2276 2292 2458 2464 2984 3274 4409
Size: 219 1231 16 166 6 520 290 1135
Cuts at: 4409 5090 5281 826
Size: 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 520 290 219 191
166 16 6
SfoI GGC'GCC
Cuts at: 2657 2657
Size: 5926
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 3253 1872
Size: 1381 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4030 4931 5193 820
Size: 197 926 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2887 3717 493
Size: 2394 830 2702
Fragments arranged by size:
2702 2394 830
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2563 3284 1132
Size: 1431 721 3774
Fragments arranged by size:
3774 1431 721
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 2174 1517
Size: 657 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2307 2844 2850 3135 3365
Size: 552 939 72 231 537 6 285 230
Cuts at: 3365 513
Size: 3074
Fragments arranged by size:
3074 939 552 537 285 231 230 72
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2410 2719 3953 3970 4123 4308 4753
Size: 1255 830 309 1234 17 153 185 445
Cuts at: 4753 325
Size: 1498
Fragments arranged by size:
1498 1255 1234 830 445 309 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4172 5884 96
Size: 274 80 33 51 1443 2195 1712 138
Fragments arranged by size:
2195 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5886 5918 2000
Size: 32 3854 32 2008
Fragments arranged by size:
3854 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2789 1316
Size: 1473 4453
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2851 2107
Size: 744 5182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 3251 1870
Size: 1381 4545
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoICRI EcoRV FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SfiI SrfI SwaI