Restriction map for
pFN21AB0944
(Circular) (Six-base) MAPSORT of: pFN21AB0944.seq Check: 3997 from: 1 to: 5884
pFN21AB0944 5884 bp
With 182 enzymes: SgfI *
March 28, 2012 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3947 5331 5599 1632
Size: 2315 1384 268 1917
Fragments arranged by size:
2315 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 2796 4783 5760 1203
Size: 1593 1987 977 1327
Fragments arranged by size:
1987 1593 1327 977
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 2095 2473 4613 2095
Size: 378 2140 3366
Fragments arranged by size:
3366 2140 378
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AlwNI CAG_nnn'CTG
Cuts at: 2359 2992 3129 3627 5199 2359
Size: 633 137 498 1572 3044
Fragments arranged by size:
3044 1572 633 498 137
ApaI G_GGCC'C
Cuts at: 3166 3166
Size: 5884
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2690 3008 3209 1870
Size: 73 244 503 318 201 4545
Fragments arranged by size:
4545 503 318 244 201 73
BamHI G'GATC_C
Cuts at: 2654 2722 3214 3581 2654
Size: 68 492 367 4957
Fragments arranged by size:
4957 492 367 68
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2341 3040 3068 3205
Size: 325 217 627 357 197 699 28 137
Cuts at: 3205 4660 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 699 627 357 325 217 197
137 28
BanII G_rGCy'C
Cuts at: 1391 2680 2853 2940 2959 3110 3166 3203 1391
Size: 1289 173 87 19 151 56 37 4072
Fragments arranged by size:
4072 1289 173 151 87 56 37 19
BbeI G_GCGC'C
Cuts at: 2148 2345 3072 2148
Size: 197 727 4960
Fragments arranged by size:
4960 727 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 2198 4495
Size: 107 122 71 631 605 365 161 2297
Cuts at: 4495 136
Size: 1525
Fragments arranged by size:
2297 1525 631 605 365 161 122 107
71
BglII A'GATC_T
Cuts at: 3151 5879 3151
Size: 2728 3156
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2135 2501 2871 3045 3166 3945 5101 1790
Size: 345 366 370 174 121 779 1156 2573
Fragments arranged by size:
2573 1156 779 370 366 345 174 121
BmgBI CAC'GTC
Cuts at: 1318 2097 1318
Size: 779 5105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2005 2095 3615
Size: 416 53 83 399 24 588 90 1520
Cuts at: 3615 4573 442
Size: 958 1753
Fragments arranged by size:
1753 1520 958 588 416 399 90 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2916 3040 3176 4544 1239
Size: 1677 124 136 1368 2579
Fragments arranged by size:
2579 1677 1368 136 124
Bpu10I CC'TnA_GC
Cuts at: 3109 3109
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4009 4874 5172 4009
Size: 865 298 4721
Fragments arranged by size:
4721 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2429 2629 2924 4547 882
Size: 617 930 200 295 1623 2219
Fragments arranged by size:
2219 1623 930 617 295 200
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2213 2342 2645
Size: 53 83 186 936 612 68 129 303
Cuts at: 2645 2810 3069 3691 4073 4708 275
Size: 165 259 622 382 635 1451
Fragments arranged by size:
1451 936 635 622 612 382 303 259
186 165 129 83 68 53
BsaWI w'CCGG_w
Cuts at: 2231 4317 4715 4989 5136 5547 2231
Size: 2086 398 274 147 411 2568
Fragments arranged by size:
2568 2086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2075 2105 3013 3043 1178
Size: 30 165 30 672 30 908 30 4019
Fragments arranged by size:
4019 908 672 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2010 2024 2098 2101 2447 1186
Size: 824 14 74 3 346 4623
Fragments arranged by size:
4623 824 346 74 14 3
BseYI C'CCAG_C
Cuts at: 2067 2073 2419 2884 2950 5087 5583 2067
Size: 6 346 465 66 2137 496 2368
Fragments arranged by size:
2368 2137 496 465 346 66 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 3051 1163
Size: 573 555 760 3996
Fragments arranged by size:
3996 760 573 555
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2349 2415 2506 3334 4095 5123
Size: 275 186 348 66 91 828 761 1028
Cuts at: 5123 1540
Size: 2301
Fragments arranged by size:
2301 1028 828 761 348 275 186 91
66
BsiHKAI G_wGCw'C
Cuts at: 2959 3110 3945 4030 5101 2959
Size: 151 835 85 1071 3742
Fragments arranged by size:
3742 1071 835 151 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2805 2366
Size: 439 5445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2501 2680 2853 2871 2940 2959
Size: 399 345 366 179 173 18 69 19
Cuts at: 2959 3045 3110 3166 3203 3945 4030 5101 1391
Size: 86 65 56 37 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 345 179 173
86 85 69 65 56 37 19 18
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 844
Size: 2395 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 3771 1394
Size: 429 446 1502 3507
Fragments arranged by size:
3507 1502 446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2344 2348 2448 4528 4715 1700
Size: 110 268 266 4 100 2080 187 2869
Fragments arranged by size:
2869 2080 268 266 187 110 100 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 2225 1925
Size: 300 5584
BssSI C'ACGA_G
Cuts at: 2309 3944 4956 2309
Size: 1635 1012 3237
Fragments arranged by size:
3237 1635 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 794
Size: 1520 4364
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstXI CCAn_nnnn'nTGG
Cuts at: 2871 2871
Size: 5884
BstYI r'GATC_y
Cuts at: 1972 2052 2654 2722 3151 3214 3581 3966 3983
Size: 80 602 68 429 63 367 385 17
Cuts at: 3983 5435 5521 5533 5879 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 602 429 385 367 346 86
80 68 63 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2149 2506 513
Size: 552 939 145 357 3891
Fragments arranged by size:
3891 939 552 357 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 2450 2913 3400 4194 4214 2450
Size: 463 487 794 20 4120
Fragments arranged by size:
4120 794 487 463 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2839 4891 809
Size: 507 1523 2052 1802
Fragments arranged by size:
2052 1802 1523 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2038 2080 2346
Size: 54 1425 50 275 18 208 42 266
Cuts at: 2346 2350 2385 2401 2412 2901 3331 4220 5849
Size: 4 35 16 11 489 430 889 1629
Cuts at: 5849 8
Size: 43
Fragments arranged by size:
1629 1425 889 489 430 275 266 208
54 50 43 42 35 18 16 11
4
EagI C'GGCC_G
Cuts at: 1537 1812 2346 2412 3331 1537
Size: 275 534 66 919 4090
Fragments arranged by size:
4090 919 534 275 66
EarI CTCTTCn'nnn_
Cuts at: 1353 3814 1353
Size: 2461 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2259 2549 2617 2653 4471 4845
Size: 1064 138 670 290 68 36 1818 374
Cuts at: 4845 4991 387
Size: 146 1280
Fragments arranged by size:
1818 1280 1064 670 374 290 146 138
68 36
EcoICRI GAG'CTC
Cuts at: 2957 3108 2957
Size: 151 5733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2916 3040 3176 3947 4544 5331 5599
Size: 393 1284 124 136 771 597 787 268
Cuts at: 5599 1239
Size: 1524
Fragments arranged by size:
1524 1284 787 771 597 393 268 136
124
EcoNI CCTnn'n_nnAGG
Cuts at: 2639 2639
Size: 5884
EcoO109I rG'GnC_Cy
Cuts at: 2475 3162 2475
Size: 687 5197
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FseI GG_CCGG'CC
Cuts at: 2082 2352 2082
Size: 270 5614
FspI TGC'GCA
Cuts at: 2282 2288 4390 2282
Size: 6 2102 3776
Fragments arranged by size:
3776 2102 6
FspAI rTGC'GCAy
Cuts at: 2288 2288
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2148 2262 2345 2698 3072 5031 1678
Size: 470 114 83 353 374 1959 2531
Fragments arranged by size:
2531 1959 470 374 353 114 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2459 2491 4525
Size: 32 135 32 509 32 346 32 2034
Cuts at: 4525 4557 4599 4631 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2034 509 346 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2144 2341 3068 2144
Size: 197 727 4960
Fragments arranged by size:
4960 727 197
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 2796 5760 2796
Size: 2964 2920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2817 4998 5182 1305
Size: 1512 2181 184 2007
Fragments arranged by size:
2181 2007 1512 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2387 10
Size: 54 1425 343 555 3507
Fragments arranged by size:
3507 1425 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3842 4201 4360 518
Size: 648 2676 359 159 2042
Fragments arranged by size:
2676 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2240 2251 2494 3300 3977 5125 5370
Size: 545 89 11 243 806 677 1148 245
Cuts at: 5370 1606
Size: 2120
Fragments arranged by size:
2120 1148 806 677 545 245 243 89
11
NaeI GCC'GGC
Cuts at: 2080 2346 2350 2450 2080
Size: 266 4 100 5514
Fragments arranged by size:
5514 266 100 4
NarI GG'CG_CC
Cuts at: 2145 2342 3069 2145
Size: 197 727 4960
Fragments arranged by size:
4960 727 197
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4393
Fragments arranged by size:
4393 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 2078 2344 2348 2448 2078
Size: 266 4 100 5514
Fragments arranged by size:
5514 266 100 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2287 3242 4787 1132
Size: 97 1058 955 1545 2229
Fragments arranged by size:
2229 1545 1058 955 97
PasI CC'CwG_GG
Cuts at: 1348 2062 2727 1348
Size: 714 665 4505
Fragments arranged by size:
4505 714 665
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2475 2475
Size: 5884
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PspOMI G'GGCC_C
Cuts at: 3162 3162
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2183 2445 2997 3236 830
Size: 1353 262 552 239 3478
Fragments arranged by size:
3478 1353 552 262 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SacI G_AGCT'C
Cuts at: 2959 3110 2959
Size: 151 5733
SacII CC_GC'GG
Cuts at: 2152 2152
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SanDI GG'GwC_CC
Cuts at: 2475 2475
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SfcI C'TryA_G
Cuts at: 826 1045 2179 2441 2993 3232 4367 5048 5239
Size: 219 1134 262 552 239 1135 681 191
Cuts at: 5239 826
Size: 1471
Fragments arranged by size:
1471 1135 1134 681 552 262 239 219
191
SfoI GGC'GCC
Cuts at: 2146 2343 3070 2146
Size: 197 727 4960
Fragments arranged by size:
4960 727 197
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3010 3211 1872
Size: 1138 201 4545
Fragments arranged by size:
4545 1138 201
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3988 4889 5151 820
Size: 197 926 2045 901 262 1553
Fragments arranged by size:
2045 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 2287 3242 1132
Size: 1155 955 3774
Fragments arranged by size:
3774 1155 955
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 3114 1517
Size: 1597 4287
StyI C'CwwG_G
Cuts at: 513 1065 2004 2337 2480 2779 2818 3323 513
Size: 552 939 333 143 299 39 505 3074
Fragments arranged by size:
3074 939 552 505 333 299 143 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2991 3911 3928 4081 4266 4711 325
Size: 1255 1411 920 17 153 185 445 1498
Fragments arranged by size:
1498 1411 1255 920 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 3013 3045 5844 5876 2075
Size: 32 906 32 2799 32 2083
Fragments arranged by size:
2799 2083 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2836 1316
Size: 1520 4364
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2868 2868
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3008 3209 1870
Size: 1138 201 4545
Fragments arranged by size:
4545 1138 201
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SapI SexAI SfiI SrfI SwaI