Restriction map for
pFN21AB0936
(Circular) (Six-base) MAPSORT of: pFN21AB0936.seq Check: 6287 from: 1 to: 5863
pFN21AB0936 5863 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 1937
Size: 1269 4594
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2216 2793 3926 5310 5578 1632
Size: 584 577 1133 1384 268 1917
Fragments arranged by size:
1917 1384 1133 584 577 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 2774 2902 1166
Size: 1608 128 4127
Fragments arranged by size:
4127 1608 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2587 2619 2587
Size: 32 5831
AlwNI CAG_nnn'CTG
Cuts at: 2239 2740 3026 3606 5178 2239
Size: 501 286 580 1572 2924
Fragments arranged by size:
2924 1572 580 501 286
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 2325 2912 3172 3398 3550 5659 1493
Size: 832 587 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 832 587 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 3188 1870
Size: 73 1245 4545
Fragments arranged by size:
4545 1245 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2415 2570 3182 1391
Size: 1024 155 612 4072
Fragments arranged by size:
4072 1024 612 155
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4474 136
Size: 107 122 71 631 605 2802 1525
Fragments arranged by size:
2802 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 3924 5080 1790
Size: 2134 1156 2573
Fragments arranged by size:
2573 2134 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 2667 3014
Size: 416 53 83 399 24 1147 103 347
Cuts at: 3014 3594 4552 442
Size: 580 958 1753
Fragments arranged by size:
1753 1147 958 580 416 399 347 103
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2403 2209
Size: 194 5669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2984 4523 1239
Size: 1745 1539 2579
Fragments arranged by size:
2579 1745 1539
Bpu10I CC'TnA_GC
Cuts at: 3081 3081
Size: 5863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2584 3121 3988 4853 5151 2584
Size: 537 867 865 298 3296
Fragments arranged by size:
3296 867 865 537 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2042 4296 4694 4968 5115 5526 2042
Size: 2254 398 274 147 411 2379
Fragments arranged by size:
2379 2254 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 1178
Size: 30 165 30 609 30 4999
Fragments arranged by size:
4999 609 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2325 2829 1186
Size: 1139 504 4220
Fragments arranged by size:
4220 1139 504
BseYI C'CCAG_C
Cuts at: 5066 5562 5066
Size: 496 5367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 3924 4009 5080 2415
Size: 1509 85 1071 3198
Fragments arranged by size:
3198 1509 1071 85
BsmI GAATG_Cn'
Cuts at: 2883 3374 3467 2883
Size: 491 93 5279
Fragments arranged by size:
5279 491 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2415 2570 3182 3924 4009 5080 1391
Size: 399 625 155 612 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 625 612 399 155 85
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2725 3218 844
Size: 1881 493 3489
Fragments arranged by size:
3489 1881 493
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2243 4358 4532 65
Size: 1183 995 2115 174 1396
Fragments arranged by size:
2115 1396 1183 995 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2392 4507 4694 1700
Size: 110 582 2115 187 2869
Fragments arranged by size:
2869 2115 582 187 110
BsrGI T'GTAC_A
Cuts at: 96 3036 96
Size: 2940 2923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5863
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2143 2143
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3436
Fragments arranged by size:
3436 1875 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 3379 4173 4193 2283
Size: 1096 794 20 3953
Fragments arranged by size:
3953 1096 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2046 2081 529
Size: 35 1482 35 4311
Fragments arranged by size:
4311 1482 35 35
DraI TTT'AAA
Cuts at: 2118 3167 2118
Size: 1049 4814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 4870 809
Size: 507 780 2774 1802
Fragments arranged by size:
2774 1802 780 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3310 4199 5828
Size: 54 1425 50 275 18 1480 889 1629
Cuts at: 5828 8
Size: 43
Fragments arranged by size:
1629 1480 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2403 3124 3129 3793 1353
Size: 882 168 721 5 664 3423
Fragments arranged by size:
3423 882 721 664 168 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2413
Size: 5863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2793 2984 3926 4523 5310 5578
Size: 393 584 577 191 942 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 942 787 597 584 577 393 268
191
EcoO109I rG'GnC_Cy
Cuts at: 2458 2576 2737 2458
Size: 118 161 5584
Fragments arranged by size:
5584 161 118
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
EcoRV GAT'ATC
Cuts at: 3118 3118
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373
Size: 32 135 32 2932 32 42 32 2626
Fragments arranged by size:
2932 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3150 3462 3150
Size: 312 5551
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3086 4977 5161 1305
Size: 1781 1891 184 2007
Fragments arranged by size:
2007 1891 1781 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4041
Fragments arranged by size:
4041 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2774 2902 3821 4180 4339 518
Size: 648 1608 128 919 359 159 2042
Fragments arranged by size:
2042 1608 919 648 359 159 128
MspA1I CmG'CkG
Cuts at: 1606 3279 3956 5104 5349 1606
Size: 1673 677 1148 245 2120
Fragments arranged by size:
2120 1673 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3436
Fragments arranged by size:
3436 1875 552
NdeI CA'TA_TG
Cuts at: 387 2172 387
Size: 1785 4078
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 3221 4766 1132
Size: 97 1992 1545 2229
Fragments arranged by size:
2229 1992 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2849 1108
Size: 1741 4122
PfoI T'CCnGG_A
Cuts at: 2863 2863
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2458 2576 2737 2458
Size: 118 161 5584
Fragments arranged by size:
5584 161 118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PstI C_TGCA'G
Cuts at: 830 3215 830
Size: 2385 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
SacI G_AGCT'C
Cuts at: 2415 2415
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 2458 2458
Size: 5863
SapI GCTCTTCn'nnn_
Cuts at: 2403 2403
Size: 5863
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 3211 4346 5027 5218 826
Size: 219 2166 1135 681 191 1471
Fragments arranged by size:
2166 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2563 3100 3967 4868 5130 820
Size: 197 926 620 537 867 901 262 1553
Fragments arranged by size:
1553 926 901 867 620 537 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2940 3302 513
Size: 552 1875 362 3074
Fragments arranged by size:
3074 1875 552 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2715 3890 3907 4060 4245 4690 325
Size: 1255 1135 1175 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1175 1135 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2557 3036 4109
Size: 274 80 33 51 1443 580 479 1073
Cuts at: 4109 5821 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1073 580 479 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2613 2613
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SfoI SrfI SwaI