Restriction map for
pFN21AB0934
(Circular) (Six-base) MAPSORT of: pFN21AB0934.seq Check: 1071 from: 1 to: 5848
pFN21AB0934 5848 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2049 2805 3191 1937
Size: 112 756 386 4594
Fragments arranged by size:
4594 756 386 112
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 3003 3911 5295 5563 1632
Size: 576 795 908 1384 268 1917
Fragments arranged by size:
1917 1384 908 795 576 268
AflII C'TTAA_G
Cuts at: 820 1017 2672 820
Size: 197 1655 3996
Fragments arranged by size:
3996 1655 197
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5848
AlwNI CAG_nnn'CTG
Cuts at: 2885 3591 5163 2885
Size: 706 1572 3570
Fragments arranged by size:
3570 1572 706
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2486 2528 2618 2693 3157 3383 3535 5644
Size: 993 42 90 75 464 226 152 2109
Cuts at: 5644 1493
Size: 1697
Fragments arranged by size:
2109 1697 993 464 226 152 90 75
42
AseI AT'TA_AT
Cuts at: 160 1032 3078 4402 160
Size: 872 2046 1324 1606
Fragments arranged by size:
2046 1606 1324 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 2402 3178 3545 2402
Size: 776 367 4705
Fragments arranged by size:
4705 776 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3169 4624 618
Size: 325 217 627 1382 1455 1842
Fragments arranged by size:
1842 1455 1382 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3167 1391
Size: 1776 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2305 2528 3039 928
Size: 971 406 223 511 3737
Fragments arranged by size:
3737 971 511 406 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2266 2300 4039 4073 1903
Size: 34 50 34 245 34 1739 34 3678
Fragments arranged by size:
3678 1739 245 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2342 3740 4950 2342
Size: 1398 1210 3240
Fragments arranged by size:
3240 1398 1210
BclI T'GATC_A
Cuts at: 1548 2610 1548
Size: 1062 4786
BfrBI ATG'CAT
Cuts at: 2135 2135
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 2965 3909 5065 1790
Size: 1175 944 1156 2573
Fragments arranged by size:
2573 1175 1156 944
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2959 3579 4537
Size: 416 53 83 399 24 1542 620 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1542 958 620 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2777
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4508 1239
Size: 3269 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2853 3973 4838 5136 2853
Size: 1120 865 298 3565
Fragments arranged by size:
3565 1120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2935 4511 882
Size: 617 1436 1576 2219
Fragments arranged by size:
2219 1576 1436 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5848
BseYI C'CCAG_C
Cuts at: 5051 5547 5051
Size: 496 5352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3909 3994 5065 3909
Size: 85 1071 4692
Fragments arranged by size:
4692 1071 85
BsmI GAATG_Cn'
Cuts at: 2078 2233 3359 3452 2078
Size: 155 1126 93 4474
Fragments arranged by size:
4474 1126 155 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2965 3167 3909 3994 5065 1391
Size: 399 1175 202 742 85 1071 2174
Fragments arranged by size:
2174 1175 1071 742 399 202 85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 2607 3737 5467 2607
Size: 1130 1730 2988
Fragments arranged by size:
2988 1730 1130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2994 3735 1394
Size: 429 1171 741 3507
Fragments arranged by size:
3507 1171 741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2791 4343 4517 65
Size: 1183 1543 1552 174 1396
Fragments arranged by size:
1552 1543 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 2686 96
Size: 2590 3258
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5848
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2972 2734
Size: 238 5610
BstYI r'GATC_y
Cuts at: 1972 2402 3178 3545 3930 3947 5399 5485 5497
Size: 430 776 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 776 430 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 4158 4178 3364
Size: 794 20 5034
Fragments arranged by size:
5034 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 2196 2331 3152 2196
Size: 135 821 4892
Fragments arranged by size:
4892 821 135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 4184 5813
Size: 54 1425 50 275 18 1465 889 1629
Cuts at: 5813 8
Size: 43
Fragments arranged by size:
1629 1465 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 1353
Size: 2425 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 3003 3911 4508 5295 5563 1239
Size: 393 576 795 908 597 787 268 1524
Fragments arranged by size:
1524 908 795 787 597 576 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2868 2868
Size: 5848
EcoRI G'AATT_C
Cuts at: 2486 3157 3535 2486
Size: 671 378 4799
Fragments arranged by size:
4799 671 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2687 2687
Size: 5848
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 4995 1678
Size: 3317 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2426 2458 2867
Size: 32 135 32 683 32 139 32 409
Cuts at: 2867 2899 4489 4521 4563 4595 1373
Size: 32 1590 32 42 32 2626
Fragments arranged by size:
2626 1590 683 409 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 4962 5146 1305
Size: 1125 2532 184 2007
Fragments arranged by size:
2532 2007 1125 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2021 2396 2606 3806 4165 4324 518
Size: 648 855 375 210 1200 359 159 2042
Fragments arranged by size:
2042 1200 855 648 375 359 210 159
MspA1I CmG'CkG
Cuts at: 1606 2186 3264 3941 5089 5334 1606
Size: 580 1078 677 1148 245 2120
Fragments arranged by size:
2120 1148 1078 677 580 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5296
NdeI CA'TA_TG
Cuts at: 387 2494 387
Size: 2107 3741
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NsiI A_TGCA'T
Cuts at: 2137 2137
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 3206 4751 1132
Size: 97 1977 1545 2229
Fragments arranged by size:
2229 1977 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2625 2625
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2898 1613
Size: 1285 4563
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 3200 830
Size: 2370 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2186 2186
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2097 3196 4331 5012 5203 826
Size: 219 1052 1099 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 1052 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 2868 3952 4853 5115 820
Size: 197 926 729 196 1084 901 262 1553
Fragments arranged by size:
1553 1084 926 901 729 262 197 196
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2810 3287 513
Size: 552 1745 477 3074
Fragments arranged by size:
3074 1745 552 477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3875 3892 4045 4230 4675 325
Size: 1255 2295 17 153 185 445 1498
Fragments arranged by size:
2295 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2686 4094 5806
Size: 274 80 33 51 1443 709 1408 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1712 1443 1408 709 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5848
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI