Restriction map for
pFN21AB0918
(Circular) (Six-base) MAPSORT of: pFN21AB0918.seq Check: 733 from: 1 to: 5698
pFN21AB0918 5698 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3508 278
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 1937
Size: 1104 4594
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2748 2897 3761 5145 5413 1632
Size: 582 534 149 864 1384 268 1917
Fragments arranged by size:
1917 1384 864 582 534 268 149
AflII C'TTAA_G
Cuts at: 820 1017 2666 820
Size: 197 1649 3852
Fragments arranged by size:
3852 1649 197
AflIII A'CryG_T
Cuts at: 1203 2135 4597 5574 1203
Size: 932 2462 977 1327
Fragments arranged by size:
2462 1327 977 932
AgeI A'CCGG_T
Cuts at: 2096 4529 2096
Size: 2433 3265
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5698
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 2139 2793 3755 4911 2139
Size: 654 962 1156 2926
Fragments arranged by size:
2926 1156 962 654
ApoI r'AATT_y
Cuts at: 1493 2336 2454 2513 2833 2859 3007 3233 3385
Size: 843 118 59 320 26 148 226 152
Cuts at: 3385 5494 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 843 320 226 152 148 118
59 26
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 3023 1870
Size: 73 87 993 4545
Fragments arranged by size:
4545 993 87 73
BamHI G'GATC_C
Cuts at: 2257 3028 3395 2257
Size: 771 367 4560
Fragments arranged by size:
4560 771 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3019 4474 618
Size: 325 217 627 258 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 3017 1391
Size: 1626 4072
BbeI G_GCGC'C
Cuts at: 2049 2049
Size: 5698
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2826 928
Size: 971 927 3800
Fragments arranged by size:
3800 971 927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5698
BfrBI ATG'CAT
Cuts at: 2867 2867
Size: 5698
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 4309 136
Size: 107 122 71 631 605 354 2283 1525
Fragments arranged by size:
2283 1525 631 605 354 122 107 71
BglII A'GATC_T
Cuts at: 2956 5693 2956
Size: 2737 2961
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2797 3759 4915 1790
Size: 353 654 962 1156 2573
Fragments arranged by size:
2573 1156 962 654 353
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4358 1239
Size: 3119 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3823 4688 4986 3823
Size: 865 298 4535
Fragments arranged by size:
4535 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3489 493
Size: 711 2285 2702
Fragments arranged by size:
2702 2285 711
BsaBI GATnn'nnATC
Cuts at: 2256 5692 2256
Size: 3436 2262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2046 3505 3887
Size: 53 83 186 936 482 31 1459 382
Cuts at: 3887 4522 275
Size: 635 1451
Fragments arranged by size:
1459 1451 936 635 482 382 186 83
53 31
BsaWI w'CCGG_w
Cuts at: 2096 4131 4529 4803 4950 5361 2096
Size: 2035 398 274 147 411 2433
Fragments arranged by size:
2433 2035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2346 2376 1178
Size: 30 165 30 943 30 4500
Fragments arranged by size:
4500 943 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2367 1186
Size: 889 292 4517
Fragments arranged by size:
4517 889 292
BseYI C'CCAG_C
Cuts at: 4901 5397 4901
Size: 496 5202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2143 2797 2939 3759 3844 4915 2043
Size: 100 654 142 820 85 1071 2826
Fragments arranged by size:
2826 1071 820 654 142 100 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2143 2797 2939 3017 3759 3844
Size: 399 253 100 654 142 78 742 85
Cuts at: 3844 4915 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 654 399 253 142 100
85 78
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2206 3587 5317 2206
Size: 1381 1730 2587
Fragments arranged by size:
2587 1730 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 844
Size: 2209 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4193 4367 65
Size: 1183 2945 174 1396
Fragments arranged by size:
2945 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 4342 4529 1700
Size: 110 286 2246 187 2869
Fragments arranged by size:
2869 2246 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 2569 96
Size: 2473 3225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 2817 4502 2817
Size: 1685 4013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 2257 2956 3028 3395 3780 3797 5249 5335
Size: 285 699 72 367 385 17 1452 86
Cuts at: 5335 5347 5693 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 699 385 367 346 285 86
72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 513
Size: 552 939 357 3850
Fragments arranged by size:
3850 939 552 357
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2482 487
Size: 683 56 1256 3703
Fragments arranged by size:
3703 1256 683 56
BtsI GCAGTG_nn'
Cuts at: 3214 4008 4028 3214
Size: 794 20 4884
Fragments arranged by size:
4884 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2861 2896 529
Size: 35 2297 35 3331
Fragments arranged by size:
3331 2297 35 35
DraI TTT'AAA
Cuts at: 2376 3002 2376
Size: 626 5072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663
Size: 54 1425 50 275 18 1315 889 1629
Cuts at: 5663 8
Size: 43
Fragments arranged by size:
1629 1425 1315 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2616 2892 3628 1353
Size: 1263 276 736 3423
Fragments arranged by size:
3423 1263 736 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2748 2897 3761 4358 5145 5413
Size: 393 582 534 149 864 597 787 268
Cuts at: 5413 1239
Size: 1524
Fragments arranged by size:
1524 864 787 597 582 534 393 268
149
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2705 2705
Size: 5698
FspI TGC'GCA
Cuts at: 4204 4204
Size: 5698
HaeII r_GCGC'y
Cuts at: 1678 2049 2084 4845 1678
Size: 371 35 2761 2531
Fragments arranged by size:
2761 2531 371 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2485 2517 2948
Size: 32 135 32 704 32 177 32 431
Cuts at: 2948 2980 4339 4371 4413 4445 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 704 431 177 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3042 3288 669
Size: 988 281 1104 246 3079
Fragments arranged by size:
3079 1104 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 3288 3288
Size: 5698
KasI G'GCGC_C
Cuts at: 2045 2045
Size: 5698
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3876
Fragments arranged by size:
3876 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2640 3656 4015 4174 518
Size: 648 1474 1016 359 159 2042
Fragments arranged by size:
2042 1474 1016 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NarI GG'CG_CC
Cuts at: 2046 2046
Size: 5698
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4207
Fragments arranged by size:
4207 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NsiI A_TGCA'T
Cuts at: 2869 2869
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2139 2483 3056 4601 1132
Size: 97 910 344 573 1545 2229
Fragments arranged by size:
2229 1545 910 573 344 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5698
PciI A'CATG_T
Cuts at: 2135 4597 2135
Size: 2462 3236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 2460 3268 2460
Size: 808 4890
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SapI GCTCTTCn'nnn_
Cuts at: 2616 2616
Size: 5698
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 3946 5658 3946
Size: 1712 3986
SfcI C'TryA_G
Cuts at: 826 1045 2806 3046 4181 4862 5053 826
Size: 219 1761 240 1135 681 191 1471
Fragments arranged by size:
1761 1471 1135 681 240 219 191
SfoI GGC'GCC
Cuts at: 2047 2047
Size: 5698
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 2096 1700
Size: 396 5302
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3802 4703 4965 820
Size: 197 926 723 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 901 723 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 3056 1132
Size: 1924 3774
SspI AAT'ATT
Cuts at: 5 52 2715 3622 5
Size: 47 2663 907 2081
Fragments arranged by size:
2663 2081 907 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 3137 513
Size: 552 939 1133 3074
Fragments arranged by size:
3074 1133 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3725 3742 3895 4080 4525 325
Size: 1255 2145 17 153 185 445 1498
Fragments arranged by size:
2145 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2569 3944 5656
Size: 274 80 33 51 1443 592 1375 1712
Cuts at: 5656 96
Size: 138
Fragments arranged by size:
1712 1443 1375 592 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2346 2378 5658 5690 2346
Size: 32 3280 32 2354
Fragments arranged by size:
3280 2354 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5698
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3506 276
Size: 53 83 186 936 1972 2468
Fragments arranged by size:
2468 1972 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI XcmI