Restriction map for
pFN21AB0916
(Circular) (Six-base) MAPSORT of: pFN21AB0916.seq Check: 2781 from: 1 to: 5689
pFN21AB0916 5689 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2803 3032 1937
Size: 866 229 4594
Fragments arranged by size:
4594 866 229
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2588 3752 5136 5404 1632
Size: 956 1164 1384 268 1917
Fragments arranged by size:
1917 1384 1164 956 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2181 2532 2921 4588 5565 1203
Size: 978 351 389 1667 977 1327
Fragments arranged by size:
1667 1327 978 977 389 351
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2872 1166
Size: 1706 3983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2890 1184
Size: 1706 3983
AlwNI CAG_nnn'CTG
Cuts at: 2177 3432 5004 2177
Size: 1255 1572 2862
Fragments arranged by size:
2862 1572 1255
ApaI G_GGCC'C
Cuts at: 2313 2313
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2216 2684 2998 3224 3376 5485 1493
Size: 723 468 314 226 152 2109 1697
Fragments arranged by size:
2109 1697 723 468 314 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2305 2362 3014 1870
Size: 73 362 57 652 4545
Fragments arranged by size:
4545 652 362 73 57
AvrII C'CTAG_G
Cuts at: 2422 2422
Size: 5689
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2697 2739 3010 4465
Size: 325 217 627 510 400 42 271 1455
Cuts at: 4465 618
Size: 1842
Fragments arranged by size:
1842 1455 627 510 400 325 271 217
42
BanII G_rGCy'C
Cuts at: 1391 2313 2464 2898 3008 1391
Size: 922 151 434 110 4072
Fragments arranged by size:
4072 922 434 151 110
BbeI G_GCGC'C
Cuts at: 2301 2301
Size: 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2065 2099 3880 3914 1903
Size: 34 128 34 1781 34 3678
Fragments arranged by size:
3678 1781 128 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 2480 2519 3106 2480
Size: 39 587 5063
Fragments arranged by size:
5063 587 39
Bme1580I G_kGCm'C
Cuts at: 1790 2313 2702 3750 4906 1790
Size: 523 389 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 523 389
BmgBI CAC'GTC
Cuts at: 1318 2182 2924 1318
Size: 864 742 4083
Fragments arranged by size:
4083 864 742
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2894 2900 3420
Size: 416 53 83 399 24 1477 6 520
Cuts at: 3420 4378 442
Size: 958 1753
Fragments arranged by size:
1753 1477 958 520 416 399 83 53
24 6
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 2729 4349 1239
Size: 1460 30 1620 2579
Fragments arranged by size:
2579 1620 1460 30
Bpu10I CC'TnA_GC
Cuts at: 2703 2703
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2284 2298 3496 3878
Size: 53 83 186 936 751 14 1198 382
Cuts at: 3878 4513 275
Size: 635 1451
Fragments arranged by size:
1451 1198 936 751 635 382 186 83
53 14
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2856 2880 2886 2910 1178
Size: 30 165 30 1453 24 6 24 3957
Fragments arranged by size:
3957 1453 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2941 2969 1186
Size: 1755 28 3906
Fragments arranged by size:
3906 1755 28
BseYI C'CCAG_C
Cuts at: 2511 2715 2735 2959 4892 5388 2511
Size: 204 20 224 1933 496 2812
Fragments arranged by size:
2812 1933 496 224 204 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2114 2464 2869 3750 3835 4906 2114
Size: 350 405 881 85 1071 2897
Fragments arranged by size:
2897 1071 881 405 350 85
BsmI GAATG_Cn'
Cuts at: 2331 3200 3293 2331
Size: 869 93 4727
Fragments arranged by size:
4727 869 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2635 2930 2635
Size: 295 5394
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2313 2464 2702 2869 2898 3008
Size: 399 324 199 151 238 167 29 110
Cuts at: 3008 3750 3835 4906 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 238 199 167
151 110 85 29
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 2730 4184 4358 65
Size: 1183 1068 414 1454 174 1396
Fragments arranged by size:
1454 1396 1183 1068 414 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2146 4333 4520 1700
Size: 110 336 2187 187 2869
Fragments arranged by size:
2869 2187 336 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2735 2971 2735
Size: 236 5453
BstYI r'GATC_y
Cuts at: 1972 2163 3019 3386 3771 3788 5240 5326 5338
Size: 191 856 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 856 385 367 346 191 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2825 513
Size: 552 939 821 3377
Fragments arranged by size:
3377 939 821 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2549 3205 3999 4019 2056
Size: 493 656 794 20 3726
Fragments arranged by size:
3726 794 656 493 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2144 2391
Size: 54 1425 50 275 18 177 137 247
Cuts at: 2391 3136 4025 5654 8
Size: 745 889 1629 43
Fragments arranged by size:
1629 1425 889 745 275 247 177 137
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2405 3619 1353
Size: 1052 1214 3423
Fragments arranged by size:
3423 1214 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2462 2462
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 2699 2729 3752 4349 5136 5404
Size: 393 956 111 30 1023 597 787 268
Cuts at: 5404 1239
Size: 1524
Fragments arranged by size:
1524 1023 956 787 597 393 268 111
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2859 2859
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2019 2174 2201 2244 2309 2310 2019
Size: 155 27 43 65 1 5398
Fragments arranged by size:
5398 155 65 43 27 1
EcoRI G'AATT_C
Cuts at: 2216 2998 3376 2216
Size: 782 378 4529
Fragments arranged by size:
4529 782 378
EcoRV GAT'ATC
Cuts at: 2908 2908
Size: 5689
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2301 4836 1678
Size: 623 2535 2531
Fragments arranged by size:
2535 2531 623
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2785 2817 2880 2912 4330
Size: 32 135 32 1213 32 63 32 1418
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1418 1213 135 63 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2092 2804 3033 3279 669
Size: 988 281 154 712 229 246 3079
Fragments arranged by size:
3079 988 712 281 246 229 154
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2297 2297
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 2387 3288 2387
Size: 901 4788
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2393 10
Size: 54 1425 343 561 3306
Fragments arranged by size:
3306 1425 561 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2872 3647 4006 4165 518
Size: 648 1706 775 359 159 2042
Fragments arranged by size:
2042 1706 775 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2695 2749 3105 3782 4930 5175 1606
Size: 1089 54 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 1089 677 356 245 54
NaeI GCC'GGC
Cuts at: 2148 2148
Size: 5689
NarI GG'CG_CC
Cuts at: 2298 2298
Size: 5689
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4198
Fragments arranged by size:
4198 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2146 2146
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2536 3047 4592 1132
Size: 97 1307 511 1545 2229
Fragments arranged by size:
2229 1545 1307 511 97
PasI CC'CwG_GG
Cuts at: 1348 2197 1348
Size: 849 4840
PciI A'CATG_T
Cuts at: 2532 4588 2532
Size: 2056 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2051 2535 1108
Size: 943 484 4262
Fragments arranged by size:
4262 943 484
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2201 2201
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2309 2309
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2243 2754 2938 3041 830
Size: 1413 511 184 103 3478
Fragments arranged by size:
3478 1413 511 184 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2749 2749
Size: 5689
SacI G_AGCT'C
Cuts at: 2464 2464
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 2802 3031 1936
Size: 866 229 4594
Fragments arranged by size:
4594 866 229
SanDI GG'GwC_CC
Cuts at: 2201 2201
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 2243 3041 2243
Size: 798 4891
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SexAI A'CCwGG_T
Cuts at: 2102 2102
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2239 2750 2934 3037 4172 4853 5044
Size: 219 1194 511 184 103 1135 681 191
Cuts at: 5044 826
Size: 1471
Fragments arranged by size:
1471 1194 1135 681 511 219 191 184
103
SfoI GGC'GCC
Cuts at: 2299 2299
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2307 3016 1872
Size: 435 709 4545
Fragments arranged by size:
4545 709 435
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 2422 3128 513
Size: 552 939 390 28 706 3074
Fragments arranged by size:
3074 939 706 552 390 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2727 3716 3733 3886 4071 4516
Size: 1255 549 598 989 17 153 185 445
Cuts at: 4516 325
Size: 1498
Fragments arranged by size:
1498 1255 989 598 549 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2856 2888 5649 5681 2202
Size: 32 622 32 2761 32 2210
Fragments arranged by size:
2761 2210 622 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2305 3014 1870
Size: 435 709 4545
Fragments arranged by size:
4545 709 435
XmnI GAAnn'nnTTC
Cuts at: 2687 3818 2687
Size: 1131 4558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I DraIII FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SapI
SfiI SrfI SwaI