Restriction map for
pFN21AB0908
(Circular) (Six-base) MAPSORT of: pFN21AB0908.seq Check: 7744 from: 1 to: 5638
pFN21AB0908 5638 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2936 2936
Size: 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2443 2667 2981 1937
Size: 506 224 314 4594
Fragments arranged by size:
4594 506 314 224
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 3701 5085 5353 1632
Size: 497 1572 1384 268 1917
Fragments arranged by size:
1917 1572 1384 497 268
AfeI AGC'GCT
Cuts at: 2852 2852
Size: 5638
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2379 1184
Size: 1195 4443
AlwNI CAG_nnn'CTG
Cuts at: 2074 3381 4953 2074
Size: 1307 1572 2759
Fragments arranged by size:
2759 1572 1307
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2287 2968 3335 2287
Size: 681 367 4590
Fragments arranged by size:
4590 681 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2957 1391
Size: 1566 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2080 2114 3829 3863 1903
Size: 34 143 34 1715 34 3678
Fragments arranged by size:
3678 1715 143 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2499 1548
Size: 951 4687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 4249 136
Size: 107 122 71 631 605 397 2180 1525
Fragments arranged by size:
2180 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2454 3055 2454
Size: 601 5037
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4855 1790
Size: 1909 1156 2573
Fragments arranged by size:
2573 1909 1156
BmgBI CAC'GTC
Cuts at: 1318 2014 1318
Size: 696 4942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3369 4327 442
Size: 416 53 83 399 24 1952 958 1753
Fragments arranged by size:
1952 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2065 2159 4298 1239
Size: 818 8 94 2139 2579
Fragments arranged by size:
2579 2139 818 94 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2298 1186
Size: 1112 4526
BseYI C'CCAG_C
Cuts at: 4841 5337 4841
Size: 496 5142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 1163
Size: 573 579 4486
Fragments arranged by size:
4486 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
BsmBI CGTCTCn'nnnn_
Cuts at: 2126 2126
Size: 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2957 3699 3784 4855 1391
Size: 399 1167 742 85 1071 2174
Fragments arranged by size:
2174 1167 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 2802 3527 5257 2802
Size: 725 1730 3183
Fragments arranged by size:
3183 1730 725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 2936 2993 844
Size: 2082 10 57 3489
Fragments arranged by size:
3489 2082 57 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 4133 4307 65
Size: 1183 1326 1559 174 1396
Fragments arranged by size:
1559 1396 1326 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 4282 4469 1700
Size: 110 1121 1351 187 2869
Fragments arranged by size:
2869 1351 1121 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 2578 1184
Size: 1394 4244
BstYI r'GATC_y
Cuts at: 1972 2287 2968 3335 3720 3737 5189 5275 5287
Size: 315 681 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 681 385 367 346 315 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 3154 3948 3968 2745
Size: 409 794 20 4415
Fragments arranged by size:
4415 794 409 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2449 2484 529
Size: 35 1885 35 3683
Fragments arranged by size:
3683 1885 35 35
DraI TTT'AAA
Cuts at: 2388 2942 2388
Size: 554 5084
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603
Size: 54 1425 50 275 18 1255 889 1629
Cuts at: 5603 8
Size: 43
Fragments arranged by size:
1629 1425 1255 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 3568 1353
Size: 912 1303 3423
Fragments arranged by size:
3423 1303 912
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4225 4599 4745 387
Size: 1064 138 435 2201 374 146 1280
Fragments arranged by size:
2201 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2065 2129 2159 3701 4298 5085
Size: 393 425 8 64 30 1542 597 787
Cuts at: 5085 5353 1239
Size: 268 1524
Fragments arranged by size:
1542 1524 787 597 425 393 268 64
30 8
EcoO109I rG'GnC_Cy
Cuts at: 2200 2839 2200
Size: 639 4999
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FspI TGC'GCA
Cuts at: 2166 4144 2166
Size: 1978 3660
HaeII r_GCGC'y
Cuts at: 1678 2075 2854 4785 1678
Size: 397 779 1931 2531
Fragments arranged by size:
2531 1931 779 397
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2681 2713 4279 4311 4353
Size: 32 135 32 1109 32 1566 32 42
Cuts at: 4353 4385 1373
Size: 32 2626
Fragments arranged by size:
2626 1566 1109 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2194 2321 2982 3228 669
Size: 988 281 256 127 661 246 3079
Fragments arranged by size:
3079 988 661 281 256 246 127
HindIII A'AGCT_T
Cuts at: 748 2684 748
Size: 1936 3702
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 4752 4936 1305
Size: 1425 2022 184 2007
Fragments arranged by size:
2022 2007 1425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3816
Fragments arranged by size:
3816 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2268 2338 3596 3955 4114 518
Size: 648 1102 70 1258 359 159 2042
Fragments arranged by size:
2042 1258 1102 648 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 2767 3054 3731 4879 5124 1606
Size: 1161 287 677 1148 245 2120
Fragments arranged by size:
2120 1161 1148 677 287 245
NaeI GCC'GGC
Cuts at: 2933 2933
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NgoMIV G'CCGG_C
Cuts at: 2931 2931
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5638
PfoI T'CCnGG_A
Cuts at: 2172 2172
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2871 2990 830
Size: 2041 119 3478
Fragments arranged by size:
3478 2041 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2767 2767
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SexAI A'CCwGG_T
Cuts at: 2807 2807
Size: 5638
SfcI C'TryA_G
Cuts at: 826 1045 2314 2867 2986 4121 4802 4993 826
Size: 219 1269 553 119 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 681 553 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2042 2156 1517
Size: 525 114 4999
Fragments arranged by size:
4999 525 114
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 3077 513
Size: 552 939 579 494 3074
Fragments arranged by size:
3074 939 579 552 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2179 3665 3682 3835 4020 4465 325
Size: 1255 599 1486 17 153 185 445 1498
Fragments arranged by size:
1498 1486 1255 599 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI