Restriction map for
pFN21AB0906
(Circular) (Six-base) MAPSORT of: pFN21AB0906.seq Check: 7969 from: 1 to: 5620
pFN21AB0906 5620 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2963 1937
Size: 1026 4594
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 3747 4120 1563
Size: 2184 373 3063
Fragments arranged by size:
3063 2184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2576 3683 5067 5335 1632
Size: 677 267 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1107 677 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2197 2281 2642 3363 4935 2197
Size: 84 361 721 1572 2882
Fragments arranged by size:
2882 1572 721 361 84
ApaLI G'TGCA_C
Cuts at: 2144 3677 4833 2144
Size: 1533 1156 2931
Fragments arranged by size:
2931 1533 1156
ApoI r'AATT_y
Cuts at: 1493 2486 2929 3155 3307 5416 1493
Size: 993 443 226 152 2109 1697
Fragments arranged by size:
2109 1697 993 443 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 2094 2945 1870
Size: 73 105 46 851 4545
Fragments arranged by size:
4545 851 105 73 46
BamHI G'GATC_C
Cuts at: 2271 2871 2950 3317 2271
Size: 600 79 367 4574
Fragments arranged by size:
4574 600 367 79
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2231 2429 2941 4396
Size: 325 217 627 311 133 198 512 1455
Cuts at: 4396 618
Size: 1842
Fragments arranged by size:
1842 1455 627 512 325 311 217 198
133
BanII G_rGCy'C
Cuts at: 1391 2049 2555 2939 1391
Size: 658 506 384 4072
Fragments arranged by size:
4072 658 506 384
BbeI G_GCGC'C
Cuts at: 2102 2102
Size: 5620
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4649
BbvCI CC'TCA_GC
Cuts at: 2535 2535
Size: 5620
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3811 3845 1903
Size: 34 1874 34 3678
Fragments arranged by size:
3678 1874 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5615 5615
Size: 5620
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 2148 2234 3681 4837 1790
Size: 358 86 1447 1156 2573
Fragments arranged by size:
2573 1447 1156 358 86
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3351 4309 442
Size: 416 53 83 399 24 1934 958 1753
Fragments arranged by size:
1934 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2189 2387 2427 4280 1239
Size: 899 51 198 40 1853 2579
Fragments arranged by size:
2579 1853 899 198 51 40
Bpu10I CC'TnA_GC
Cuts at: 2535 2535
Size: 5620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3745 4610 4908 3745
Size: 865 298 4457
Fragments arranged by size:
4457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 4283 882
Size: 617 666 2118 2219
Fragments arranged by size:
2219 2118 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 3411 493
Size: 711 1290 917 2702
Fragments arranged by size:
2702 1290 917 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 3427 3809 4444
Size: 53 83 186 936 566 1328 382 635
Cuts at: 4444 275
Size: 1451
Fragments arranged by size:
1451 1328 936 635 566 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2874 4053 4451 4725 4872 5283 2874
Size: 1179 398 274 147 411 3211
Fragments arranged by size:
3211 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2234 2264 1178
Size: 30 165 30 831 30 4534
Fragments arranged by size:
4534 831 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2552 2585 2660 1186
Size: 949 417 33 75 4146
Fragments arranged by size:
4146 949 417 75 33
BseYI C'CCAG_C
Cuts at: 2446 2856 4823 5319 2446
Size: 410 1967 496 2747
Fragments arranged by size:
2747 1967 496 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 2819 1163
Size: 573 780 303 3964
Fragments arranged by size:
3964 780 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 2130 3070 3831 4859 1540
Size: 275 186 105 24 940 761 1028 2301
Fragments arranged by size:
2301 1028 940 761 275 186 105 24
BsiHKAI G_wGCw'C
Cuts at: 2148 2255 3681 3766 4837 2148
Size: 107 1426 85 1071 2931
Fragments arranged by size:
2931 1426 1071 107 85
BsmI GAATG_Cn'
Cuts at: 2376 3131 3224 2376
Size: 755 93 4772
Fragments arranged by size:
4772 755 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2148 2234 2255 2555 2939 3681
Size: 399 259 99 86 21 300 384 742
Cuts at: 3681 3766 4837 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 300 259 99
86 85 21
BspEI T'CCGG_A
Cuts at: 2874 5283 2874
Size: 2409 3211
BspHI T'CATG_A
Cuts at: 3509 5239 3509
Size: 1730 3890
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2183 2975 844
Size: 1339 792 3489
Fragments arranged by size:
3489 1339 792
BsrBI CCG'CTC
Cuts at: 1394 1823 2182 2575 3507 1394
Size: 429 359 393 932 3507
Fragments arranged by size:
3507 932 429 393 359
BsrDI GCAATG_nn'
Cuts at: 65 1248 4115 4289 65
Size: 1183 2867 174 1396
Fragments arranged by size:
2867 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2101 4264 4451 1700
Size: 110 264 27 2163 187 2869
Fragments arranged by size:
2869 2163 264 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 2080 1925
Size: 155 5465
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2503 794
Size: 1709 3911
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2271 2871 2950 3317 3702 3719 5171 5257
Size: 299 600 79 367 385 17 1452 86
Cuts at: 5257 5269 5615 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 600 385 367 346 299 86
79 17 12
Bsu36I CC'TnA_GG
Cuts at: 2039 2039
Size: 5620
BtgI C'CryG_G
Cuts at: 513 1065 2106 2211 2226 513
Size: 552 1041 105 15 3907
Fragments arranged by size:
3907 1041 552 105 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 3136 3930 3950 3136
Size: 794 20 4806
Fragments arranged by size:
4806 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DraIII CAC_nnn'GTG
Cuts at: 2212 2212
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2127 2199
Size: 54 1425 50 275 18 273 24 72
Cuts at: 2199 3067 3956 5585 8
Size: 868 889 1629 43
Fragments arranged by size:
1629 1425 889 868 275 273 72 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2103 2127 3067 1537
Size: 275 291 24 940 4090
Fragments arranged by size:
4090 940 291 275 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2867 3550 1353
Size: 650 864 683 3423
Fragments arranged by size:
3423 864 683 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2189 2309 2387 2427 2576 3683
Size: 393 506 51 120 78 40 149 1107
Cuts at: 3683 4280 5067 5335 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1107 787 597 506 393 268 149
120 78 51 40
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 5620
EcoO109I rG'GnC_Cy
Cuts at: 2054 2278 2320 2338 2054
Size: 224 42 18 5336
Fragments arranged by size:
5336 224 42 18
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 2063 4126 2063
Size: 2063 3557
HaeII r_GCGC'y
Cuts at: 1678 2102 2168 4767 1678
Size: 424 66 2599 2531
Fragments arranged by size:
2599 2531 424 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2776 2797 2808 2829 4261
Size: 32 135 32 1204 21 11 21 1432
Cuts at: 4261 4293 4335 4367 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1432 1204 135 42 32 32 32
32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KasI G'GCGC_C
Cuts at: 2098 2098
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 10
Size: 54 1425 343 369 3429
Fragments arranged by size:
3429 1425 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3578 3937 4096 518
Size: 648 2412 359 159 2042
Fragments arranged by size:
2412 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2228 2500 2868 3036 3713 4861 5106 1606
Size: 622 272 368 168 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 622 368 272 245 168
NaeI GCC'GGC
Cuts at: 2103 2103
Size: 5620
NarI GG'CG_CC
Cuts at: 2099 2099
Size: 5620
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NgoMIV G'CCGG_C
Cuts at: 2101 2101
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2978 4523 1132
Size: 97 1749 1545 2229
Fragments arranged by size:
2229 1749 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 2281 2471 1108
Size: 1104 69 190 4257
Fragments arranged by size:
4257 1104 190 69
PfoI T'CCnGG_A
Cuts at: 2364 2364
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PmlI CAC'GTG
Cuts at: 2494 2494
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2054 2320 2338 2054
Size: 266 18 5336
Fragments arranged by size:
5336 266 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5620
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2032 2560 2972 830
Size: 1202 528 412 3478
Fragments arranged by size:
3478 1202 528 412
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
SacII CC_GC'GG
Cuts at: 2229 2229
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SanDI GG'GwC_CC
Cuts at: 2054 2054
Size: 5620
SapI GCTCTTCn'nnn_
Cuts at: 2003 2867 2003
Size: 864 4756
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SexAI A'CCwGG_T
Cuts at: 2334 2334
Size: 5620
SfcI C'TryA_G
Cuts at: 826 1045 2028 2556 2968 4103 4784 4975 826
Size: 219 983 528 412 1135 681 191 1471
Fragments arranged by size:
1471 1135 983 681 528 412 219 191
SfoI GGC'GCC
Cuts at: 2100 2100
Size: 5620
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2096 2947 1872
Size: 224 851 4545
Fragments arranged by size:
4545 851 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3724 4625 4887 820
Size: 197 926 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SrfI GCCC'GGGC
Cuts at: 2096 2096
Size: 5620
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2478 3059 513
Size: 552 1413 581 3074
Fragments arranged by size:
3074 1413 581 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2417 3647 3664 3817 4002 4447 325
Size: 1255 837 1230 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1230 837 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 3866 5578
Size: 274 80 33 51 1443 610 1279 1712
Cuts at: 5578 96
Size: 138
Fragments arranged by size:
1712 1443 1279 610 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2234 2266 5580 5612 2234
Size: 32 3314 32 2242
Fragments arranged by size:
3314 2242 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2209
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2094 2945 1870
Size: 224 851 4545
Fragments arranged by size:
4545 851 224
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspOMI PspXI PsrI PvuII
RsrII SacI SfiI SwaI