Restriction map for
pFN21AB0904
(Circular) (Six-base) MAPSORT of: pFN21AB0904.seq Check: 9628 from: 1 to: 5602
pFN21AB0904 5602 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2945 1937
Size: 1008 4594
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2197 2512 3665 5049 5317 1632
Size: 461 104 315 1153 1384 268 1917
Fragments arranged by size:
1917 1384 1153 461 315 268 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AlwNI CAG_nnn'CTG
Cuts at: 2591 3345 4917 2591
Size: 754 1572 3276
Fragments arranged by size:
3276 1572 754
ApaI G_GGCC'C
Cuts at: 2052 2479 2635 2052
Size: 427 156 5019
Fragments arranged by size:
5019 427 156
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2927 1870
Size: 73 109 875 4545
Fragments arranged by size:
4545 875 109 73
AvrII C'CTAG_G
Cuts at: 2374 2374
Size: 5602
BamHI G'GATC_C
Cuts at: 2217 2932 3299 2217
Size: 715 367 4520
Fragments arranged by size:
4520 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2923 4378 618
Size: 325 217 627 306 830 1455 1842
Fragments arranged by size:
1842 1455 830 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2052 2479 2635 2921 1391
Size: 661 427 156 286 4072
Fragments arranged by size:
4072 661 427 286 156
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5602
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2494 3494 4704 2494
Size: 1000 1210 3392
Fragments arranged by size:
3392 1210 1000
BclI T'GATC_A
Cuts at: 1548 2463 1548
Size: 915 4687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2047 2630 4213
Size: 107 122 71 631 605 375 583 1583
Cuts at: 4213 136
Size: 1525
Fragments arranged by size:
1583 1525 631 605 583 375 122 107
71
BglII A'GATC_T
Cuts at: 5597 5597
Size: 5602
BlpI GC'TnA_GC
Cuts at: 2564 3019 2564
Size: 455 5147
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2066 2145 2479 2635 3663 4819 1790
Size: 262 14 79 334 156 1028 1156 2573
Fragments arranged by size:
2573 1156 1028 334 262 156 79 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3333 4291 442
Size: 416 53 83 399 24 1916 958 1753
Fragments arranged by size:
1916 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4262 1239
Size: 3023 2579
Bpu10I CC'TnA_GC
Cuts at: 2346 2346
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 3727 4592 4890 2411
Size: 1316 865 298 3123
Fragments arranged by size:
3123 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3393 493
Size: 711 2189 2702
Fragments arranged by size:
2702 2189 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3409 3791 4426
Size: 53 83 186 936 561 1315 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1315 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4035 4433 4707 4854 5265 4035
Size: 398 274 147 411 4372
Fragments arranged by size:
4372 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5377
Fragments arranged by size:
5377 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2266 1186
Size: 1080 4522
BseYI C'CCAG_C
Cuts at: 2083 2469 2613 2625 4805 5301 2083
Size: 386 144 12 2180 496 2384
Fragments arranged by size:
2384 2180 496 386 144 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2198 2702 1163
Size: 573 462 504 4063
Fragments arranged by size:
4063 573 504 462
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2796 3052 3813 4841 1540
Size: 275 186 795 256 761 1028 2301
Fragments arranged by size:
2301 1028 795 761 275 256 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3663 3748 4819 2427
Size: 1236 85 1071 3210
Fragments arranged by size:
3210 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2066 2145 2427 2479 2635 2921
Size: 399 262 14 79 282 52 156 286
Cuts at: 2921 3663 3748 4819 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 282 262 156
85 79 52 14
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3489 1394
Size: 429 1666 3507
Fragments arranged by size:
3507 1666 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4246 4433 1700
Size: 110 280 2156 187 2869
Fragments arranged by size:
2869 2156 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 2608
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2217 2932 3299 3684 3701 5153 5239 5251
Size: 245 715 367 385 17 1452 86 12
Cuts at: 5251 5597 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 715 385 367 346 245 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2097 2130 513
Size: 552 939 93 33 3985
Fragments arranged by size:
3985 939 552 93 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2439 2550
Size: 54 1425 50 275 18 366 243 111
Cuts at: 2550 3049 3938 5567 8
Size: 499 889 1629 43
Fragments arranged by size:
1629 1425 889 499 366 275 243 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2486 4189 4563 4709 387
Size: 1064 138 897 1703 374 146 1280
Fragments arranged by size:
1703 1280 1064 897 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2197 2512 3665 4262 5049 5317
Size: 393 461 104 315 1153 597 787 268
Cuts at: 5317 1239
Size: 1524
Fragments arranged by size:
1524 1153 787 597 461 393 315 268
104
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 2888 2198
Size: 690 4912
EcoO109I rG'GnC_Cy
Cuts at: 2049 2203 2049
Size: 154 5448
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2097 2374 4749 1678
Size: 419 277 2375 2531
Fragments arranged by size:
2531 2375 419 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373
Size: 32 135 32 2671 32 42 32 2626
Fragments arranged by size:
2671 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2416 2946 3192 669
Size: 988 281 478 530 246 3079
Fragments arranged by size:
3079 988 530 478 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 2552 10
Size: 54 1425 343 609 111 3060
Fragments arranged by size:
3060 1425 609 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2461 2473 2588 2629 2668 2828 3018 3695
Size: 855 12 115 41 39 160 190 677
Cuts at: 3695 4843 5088 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 855 677 245 190 160 115
41 39 12
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 5602
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5602
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4111
Fragments arranged by size:
4111 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2365 2960 4505 1132
Size: 97 1136 595 1545 2229
Fragments arranged by size:
2229 1545 1136 595 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 4069
PfoI T'CCnGG_A
Cuts at: 2538 2538
Size: 5602
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 5184 5216 3671
Size: 32 1481 32 4057
Fragments arranged by size:
4057 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2048 2475 2631 2048
Size: 427 156 5019
Fragments arranged by size:
5019 427 156
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 5570
PstI C_TGCA'G
Cuts at: 830 2954 830
Size: 2124 3478
PvuI CG_AT'CG
Cuts at: 2001 2796 2001
Size: 795 4807
PvuII CAG'CTG
Cuts at: 2461 2588 2828 2461
Size: 127 240 5235
Fragments arranged by size:
5235 240 127
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2283 2300 2592 2849 2950 4085 4766
Size: 219 1238 17 292 257 101 1135 681
Cuts at: 4766 4957 826
Size: 191 1471
Fragments arranged by size:
1471 1238 1135 681 292 257 219 191
101 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 2630 2630
Size: 5602
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2054 2929 1872
Size: 182 875 4545
Fragments arranged by size:
4545 875 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 3706 4607 4869 820
Size: 197 926 483 1280 901 262 1553
Fragments arranged by size:
1553 1280 926 901 483 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2960 1132
Size: 1828 3774
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2222 2374 2520 3041 513
Size: 552 939 218 152 146 521 3074
Fragments arranged by size:
3074 939 552 521 218 152 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2483 3629 3646 3799 3984 4429 325
Size: 1255 903 1146 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1146 903 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2580 3848 5560
Size: 274 80 33 51 1443 603 1268 1712
Cuts at: 5560 96
Size: 138
Fragments arranged by size:
1712 1443 1268 603 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5602
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2052 2927 1870
Size: 182 875 4545
Fragments arranged by size:
4545 875 182
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
RsrII SacI SacII SanDI SapI SexAI SrfI SwaI
XcmI