Restriction map for
pFN21AB0902
(Circular) (Six-base) MAPSORT of: pFN21AB0902.seq Check: 552 from: 1 to: 5596
pFN21AB0902 5596 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2558 2558
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2355 2445 2939 1937
Size: 418 90 494 4594
Fragments arranged by size:
4594 494 418 90
Acc65I G'GTAC_C
Cuts at: 2917 2917
Size: 5596
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2837 3659 5043 5311 1632
Size: 398 807 822 1384 268 1917
Fragments arranged by size:
1917 1384 822 807 398 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2576 4495 5472 1203
Size: 1373 1919 977 1327
Fragments arranged by size:
1919 1373 1327 977
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 2095 4325 2095
Size: 2230 3366
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2905 3131 3283 5392 1493
Size: 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2921 1870
Size: 73 978 4545
Fragments arranged by size:
4545 978 73
AvrII C'CTAG_G
Cuts at: 2410 2410
Size: 5596
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2917 4372 618
Size: 325 217 627 1130 1455 1842
Fragments arranged by size:
1842 1455 1130 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2915 1391
Size: 1524 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3488 4698 2511
Size: 977 1210 3409
Fragments arranged by size:
3409 1210 977
BclI T'GATC_A
Cuts at: 1548 2671 1548
Size: 1123 4473
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 2115 2642 3657 4813 1790
Size: 325 527 1015 1156 2573
Fragments arranged by size:
2573 1156 1015 527 325
BmgBI CAC'GTC
Cuts at: 1318 2579 1318
Size: 1261 4335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2551 3327 4285
Size: 416 53 83 399 24 1134 776 958
Cuts at: 4285 442
Size: 1753
Fragments arranged by size:
1753 1134 958 776 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4256 1239
Size: 3017 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 3721 4586 4884 2440
Size: 1281 865 298 3152
Fragments arranged by size:
3152 1281 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2416 4259 882
Size: 617 917 1843 2219
Fragments arranged by size:
2219 1843 917 617
BsaAI yAC'GTr
Cuts at: 493 1204 3387 493
Size: 711 2183 2702
Fragments arranged by size:
2702 2183 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3403 3785 4420 275
Size: 53 83 186 936 1870 382 635 1451
Fragments arranged by size:
1870 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2661 4029 4427 4701 4848 5259 2661
Size: 1368 398 274 147 411 2998
Fragments arranged by size:
2998 1368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2192 2210 2213 2216 2387 2810 1186
Size: 991 15 18 3 3 171 423 3972
Fragments arranged by size:
3972 991 423 171 18 15 3 3
BseYI C'CCAG_C
Cuts at: 2027 2139 4799 5295 2027
Size: 112 2660 496 2328
Fragments arranged by size:
2660 2328 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 3934
Fragments arranged by size:
3934 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3657 3742 4813 3657
Size: 85 1071 4440
Fragments arranged by size:
4440 1071 85
BsmI GAATG_Cn'
Cuts at: 2278 2871 3107 3200 2278
Size: 593 236 93 4674
Fragments arranged by size:
4674 593 236 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2642 2915 3657 3742 4813 1391
Size: 399 325 527 273 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 399 325 273 85
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 3485 5215 3485
Size: 1730 3866
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2558 2951 844
Size: 1714 393 3489
Fragments arranged by size:
3489 1714 393
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5596
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2782 794
Size: 1988 3608
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2743 2074
Size: 669 4927
BstYI r'GATC_y
Cuts at: 1972 2926 3293 3678 3695 5147 5233 5245 5591
Size: 954 367 385 17 1452 86 12 346
Cuts at: 5591 1972
Size: 1977
Fragments arranged by size:
1977 1452 954 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3883
Fragments arranged by size:
3883 939 552 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4857
Fragments arranged by size:
4857 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3906 3926 3112
Size: 794 20 4782
Fragments arranged by size:
4782 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2823 2858 529
Size: 35 2259 35 3267
Fragments arranged by size:
3267 2259 35 35
DraI TTT'AAA
Cuts at: 2689 2900 2689
Size: 211 5385
DraIII CAC_nnn'GTG
Cuts at: 2782 2782
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2607 3043 3932
Size: 54 1425 50 275 18 777 436 889
Cuts at: 3932 5561 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 777 436 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 2382 2487 2717 3526 1353
Size: 924 105 105 230 809 3423
Fragments arranged by size:
3423 924 809 230 105 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2837 3659 4256 5043 5311 1239
Size: 393 398 807 822 597 787 268 1524
Fragments arranged by size:
1524 822 807 787 597 398 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 5596
EcoO109I rG'GnC_Cy
Cuts at: 2089 2089
Size: 5596
EcoRI G'AATT_C
Cuts at: 2905 3283 2905
Size: 378 5218
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2656 2688 4237
Size: 32 135 32 509 32 543 32 1549
Cuts at: 4237 4269 4311 4343 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1549 543 509 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2921 2921
Size: 5596
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 4710 4894 1305
Size: 1503 1902 184 2007
Fragments arranged by size:
2007 1902 1503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2609 10
Size: 54 1425 343 777 2997
Fragments arranged by size:
2997 1425 777 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2741 3554 3913 4072 518
Size: 648 1575 813 359 159 2042
Fragments arranged by size:
2042 1575 813 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2143 2605 3012 3689 4837 5082 1606
Size: 500 37 462 407 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 500 462 407 245 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 2226 513
Size: 552 939 222 3883
Fragments arranged by size:
3883 939 552 222
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2683 2954 4499 1132
Size: 97 1454 271 1545 2229
Fragments arranged by size:
2229 1545 1454 271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5596
PciI A'CATG_T
Cuts at: 4495 4495
Size: 5596
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PfoI T'CCnGG_A
Cuts at: 2759 2759
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2304 2336 3665 3697 5178 5210 2304
Size: 32 1329 32 1481 32 2690
Fragments arranged by size:
2690 1481 1329 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2089 2089
Size: 5596
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2948 830
Size: 2118 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2106 2143 2605 2106
Size: 37 462 5097
Fragments arranged by size:
5097 462 37
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SapI GCTCTTCn'nnn_
Cuts at: 2487 2487
Size: 5596
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2944 4079 4760 4951 826
Size: 219 1899 1135 681 191 1471
Fragments arranged by size:
1899 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2923 1872
Size: 1051 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 3700 4601 4863 820
Size: 197 926 476 1281 901 262 1553
Fragments arranged by size:
1553 1281 926 901 476 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 2885 1517
Size: 1368 4228
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 2410 3035 513
Size: 552 939 222 184 625 3074
Fragments arranged by size:
3074 939 625 552 222 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3842 5554 96
Size: 274 80 33 51 1443 1865 1712 138
Fragments arranged by size:
1865 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 5556 5588 2045
Size: 32 3479 32 2053
Fragments arranged by size:
3479 2053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2602 2602
Size: 5596
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2921 1870
Size: 1051 4545
XmnI GAAnn'nnTTC
Cuts at: 2711 2871 3725 2711
Size: 160 854 4582
Fragments arranged by size:
4582 854 160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI