Restriction map for
pFN21AB0498
(Circular) (Six-base) MAPSORT of: pFN21AB0498.seq Check: 8509 from: 1 to: 5959
pFN21AB0498 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2656 2656
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 2477 4022 5406 5674 1632
Size: 737 108 1545 1384 268 1917
Fragments arranged by size:
1917 1545 1384 737 268 108
AfeI AGC'GCT
Cuts at: 3181 3181
Size: 5959
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2143 3702 5274 2143
Size: 1559 1572 2828
Fragments arranged by size:
2828 1572 1559
ApaI G_GGCC'C
Cuts at: 2150 2150
Size: 5959
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2220 2966 4513 160
Size: 872 1188 746 1547 1606
Fragments arranged by size:
1606 1547 1188 872 746
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2150 2467 2741 3278 1391
Size: 759 317 274 537 4072
Fragments arranged by size:
4072 759 537 317 274
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2988 928
Size: 971 1089 3899
Fragments arranged by size:
3899 1089 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2816 2850 4150 4184 1903
Size: 34 879 34 1300 34 3678
Fragments arranged by size:
3678 1300 879 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2587 5954 2587
Size: 3367 2592
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 2150 2260 4020 5176 1790
Size: 360 110 1760 1156 2573
Fragments arranged by size:
2573 1760 1156 360 110
BmgBI CAC'GTC
Cuts at: 1318 2039 1318
Size: 721 5238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3214 3690 4648
Size: 416 53 83 399 24 1797 476 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1797 1753 958 476 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2951 4619 1239
Size: 1712 1668 2579
Fragments arranged by size:
2579 1712 1668
Bpu10I CC'TnA_GC
Cuts at: 2735 2735
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2943 4084 4949 5247 2384
Size: 559 1141 865 298 3096
Fragments arranged by size:
3096 1141 865 559 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2317 3750 493
Size: 711 1113 1433 2702
Fragments arranged by size:
2702 1433 1113 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 1178
Size: 30 165 30 1305 30 4399
Fragments arranged by size:
4399 1305 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 1186
Size: 1435 4524
BseYI C'CCAG_C
Cuts at: 2143 2253 2687 3055 5162 5658 2143
Size: 110 434 368 2107 496 2444
Fragments arranged by size:
2444 2107 496 434 368 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2633 3409 4170 5198 1540
Size: 275 186 632 776 761 1028 2301
Fragments arranged by size:
2301 1028 776 761 632 275 186
BsiHKAI G_wGCw'C
Cuts at: 2741 4020 4105 5176 2741
Size: 1279 85 1071 3524
Fragments arranged by size:
3524 1279 1071 85
BsmI GAATG_Cn'
Cuts at: 2488 3470 3563 2488
Size: 982 93 4884
Fragments arranged by size:
4884 982 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2260 2467 2741 3278 4020 4105
Size: 399 360 110 207 274 537 742 85
Cuts at: 4105 5176 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 537 399 360 274 207
110 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2365 3848 5578 2365
Size: 1483 1730 2746
Fragments arranged by size:
2746 1730 1483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2656 2821 3314 844
Size: 1812 165 493 3489
Fragments arranged by size:
3489 1812 493 165
BsrBI CCG'CTC
Cuts at: 1394 1823 2815 3846 1394
Size: 429 992 1031 3507
Fragments arranged by size:
3507 1031 992 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2704 4603 4790 1700
Size: 110 894 1899 187 2869
Fragments arranged by size:
2869 1899 894 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 2668 3097 1184
Size: 1484 429 4046
Fragments arranged by size:
4046 1484 429
BstYI r'GATC_y
Cuts at: 1972 2587 3155 3289 3656 4041 4058 5510 5596
Size: 615 568 134 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 615 568 385 367 346 134
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2132 2468 2132
Size: 336 5623
BtgI C'CryG_G
Cuts at: 513 1065 2748 513
Size: 552 1683 3724
Fragments arranged by size:
3724 1683 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2529 2564 529
Size: 35 1965 35 3924
Fragments arranged by size:
3924 1965 35 35
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 3406 4295
Size: 54 1425 50 275 18 396 1180 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1180 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2573 2855 3215 3889 1353
Size: 747 473 282 360 674 3423
Fragments arranged by size:
3423 747 674 473 360 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2193 2723 2734 4546 4920 5066
Size: 1064 138 604 530 11 1812 374 146
Cuts at: 5066 387
Size: 1280
Fragments arranged by size:
1812 1280 1064 604 530 374 146 138
11
EcoICRI GAG'CTC
Cuts at: 2739 2739
Size: 5959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2477 2951 4022 4619 5406 5674
Size: 393 737 108 474 1071 597 787 268
Cuts at: 5674 1239
Size: 1524
Fragments arranged by size:
1524 1071 787 737 597 474 393 268
108
EcoO109I rG'GnC_Cy
Cuts at: 2128 2395 3163 2128
Size: 267 768 4924
Fragments arranged by size:
4924 768 267
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2045 2960 2045
Size: 915 5044
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2876 3183 5106 1678
Size: 1198 307 1923 2531
Fragments arranged by size:
2531 1923 1198 307
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 2708 2740 4600
Size: 32 135 32 1000 32 104 32 1860
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1860 1000 135 104 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 3303 3549 669
Size: 988 281 355 1010 246 3079
Fragments arranged by size:
3079 1010 988 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2534 748
Size: 1786 4173
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 10
Size: 54 1425 343 396 3741
Fragments arranged by size:
3741 1425 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2370 3917 4276 4435 518
Size: 648 1204 1547 359 159 2042
Fragments arranged by size:
2042 1547 1204 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2625 2659 2773 2794 2855 3375 4052
Size: 741 278 34 114 21 61 520 677
Cuts at: 4052 5200 5445 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 520 278 245 114
61 34 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2644 1348
Size: 1296 4663
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2808 2928 2808
Size: 120 5839
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3163 3163
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2146 2146
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2664 2723 2799 3311 830
Size: 1834 59 76 512 3478
Fragments arranged by size:
3478 1834 512 76 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2347 2625 2659 2794 2855 2347
Size: 278 34 135 61 5451
Fragments arranged by size:
5451 278 135 61 34
SacI G_AGCT'C
Cuts at: 2741 2741
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 3215 3215
Size: 5959
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2660 2719 2795 3307 4442 5123 5314
Size: 219 1615 59 76 512 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1615 1471 1135 681 512 219 191 76
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2958 4063 4964 5226 820
Size: 197 926 456 559 1105 901 262 1553
Fragments arranged by size:
1553 1105 926 901 559 456 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2406 3398 513
Size: 552 1341 992 3074
Fragments arranged by size:
3074 1341 992 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2647 2667 3986 4003 4156 4341 4786
Size: 1255 1067 20 1319 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1319 1255 1067 445 185 153 20
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 4205 5917
Size: 274 80 33 51 1443 117 2111 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
2111 1712 1443 274 138 117 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3044 3295 3044
Size: 251 5708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2351 2054
Size: 297 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2025 2939 4088 2025
Size: 914 1149 3896
Fragments arranged by size:
3896 1149 914
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI