Restriction map for
pFN21AB0496
(Circular) (Six-base) MAPSORT of: pFN21AB0496.seq Check: 4237 from: 1 to: 7831
pFN21AB0496 7831 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3053 3053
Size: 7831
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5641 278
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4460 5174 1937
Size: 2523 714 4594
Fragments arranged by size:
4594 2523 714
Acc65I G'GTAC_C
Cuts at: 5152 5152
Size: 7831
AclI AA'CG_TT
Cuts at: 1563 2143 4309 5958 6331 1563
Size: 580 2166 1649 373 3063
Fragments arranged by size:
3063 2166 1649 580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3001 3441 4541 5894 7278 7546 1632
Size: 654 715 440 1100 1353 1384 268 1917
Fragments arranged by size:
1917 1384 1353 1100 715 654 440 268
AflII C'TTAA_G
Cuts at: 820 1017 2272 2539 3659 820
Size: 197 1255 267 1120 4992
Fragments arranged by size:
4992 1255 1120 267 197
AflIII A'CryG_T
Cuts at: 1203 3331 3727 3753 4570 6730 7707 1203
Size: 2128 396 26 817 2160 977 1327
Fragments arranged by size:
2160 2128 1327 977 817 396 26
AgeI A'CCGG_T
Cuts at: 6662 6662
Size: 7831
AhdI GACnn_n'nnGTC
Cuts at: 6560 6560
Size: 7831
AleI CACnn'nnGTG
Cuts at: 1166 3372 1166
Size: 2206 5625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3307 4833 1184
Size: 2123 1526 4182
Fragments arranged by size:
4182 2123 1526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2399 2431 2399
Size: 32 7799
AlwNI CAG_nnn'CTG
Cuts at: 2123 4145 5574 7146 2123
Size: 2022 1429 1572 2808
Fragments arranged by size:
2808 2022 1572 1429
ApaLI G'TGCA_C
Cuts at: 5888 7044 5888
Size: 1156 6675
ApoI r'AATT_y
Cuts at: 1493 2618 2763 3157 3408 3512 3823 4030 4129
Size: 1125 145 394 251 104 311 207 99
Cuts at: 4129 4444 4753 5069 5140 5366 5518 7627 1493
Size: 315 309 316 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 1125 394 316 315 311 309
251 226 207 152 145 104 99 71
AseI AT'TA_AT
Cuts at: 160 1032 2658 3741 6385 160
Size: 872 1626 1083 2644 1606
Fragments arranged by size:
2644 1626 1606 1083 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
AvaI C'yCGr_G
Cuts at: 1870 1943 5156 1870
Size: 73 3213 4545
Fragments arranged by size:
4545 3213 73
BamHI G'GATC_C
Cuts at: 4075 5161 5528 4075
Size: 1086 367 6378
Fragments arranged by size:
6378 1086 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4998 5152 6607 618
Size: 325 217 627 3211 154 1455 1842
Fragments arranged by size:
3211 1842 1455 627 325 217 154
BanII G_rGCy'C
Cuts at: 1391 3638 3668 4662 5150 1391
Size: 2247 30 994 488 4072
Fragments arranged by size:
4072 2247 994 488 30
BbeI G_GCGC'C
Cuts at: 5002 5002
Size: 7831
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2274 928
Size: 971 375 6485
Fragments arranged by size:
6485 971 375
BbvCI CC'TCA_GC
Cuts at: 2494 2494
Size: 7831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3035 3069 6022 6056 1903
Size: 34 1098 34 2953 34 3678
Fragments arranged by size:
3678 2953 1098 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5723 6933 5723
Size: 1210 6621
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6442 136
Size: 107 122 71 631 605 4770 1525
Fragments arranged by size:
4770 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7826 7826
Size: 7831
BlpI GC'TnA_GC
Cuts at: 5248 5248
Size: 7831
Bme1580I G_kGCm'C
Cuts at: 1790 5892 7048 1790
Size: 4102 1156 2573
Fragments arranged by size:
4102 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4187 5562 6520
Size: 416 53 83 399 24 2770 1375 958
Cuts at: 6520 442
Size: 1753
Fragments arranged by size:
2770 1753 1375 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 7831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 4950 6491 1239
Size: 861 2850 1541 2579
Fragments arranged by size:
2850 2579 1541 861
Bpu10I CC'TnA_GC
Cuts at: 2403 2494 2403
Size: 91 7740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5956 6821 7119 5956
Size: 865 298 6668
Fragments arranged by size:
6668 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3075 3446 6494 882
Size: 617 1576 371 3048 2219
Fragments arranged by size:
3048 2219 1576 617 371
BsaAI yAC'GTr
Cuts at: 493 1204 3728 5622 493
Size: 711 2524 1894 2702
Fragments arranged by size:
2702 2524 1894 711
BsaBI GATnn'nnATC
Cuts at: 7825 7825
Size: 7831
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4999 5638 6020 6655
Size: 53 83 186 936 3466 639 382 635
Cuts at: 6655 275
Size: 1451
Fragments arranged by size:
3466 1451 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6264 6662 6936 7083 7494 6264
Size: 398 274 147 411 6601
Fragments arranged by size:
6601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4011 4041 1178
Size: 30 165 30 2608 30 4968
Fragments arranged by size:
4968 2608 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7831
BseYI C'CCAG_C
Cuts at: 4014 7034 7530 4014
Size: 3020 496 4315
Fragments arranged by size:
4315 3020 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2469 1163
Size: 573 733 6525
Fragments arranged by size:
6525 733 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4259 5281 6042 7070 1540
Size: 275 186 2258 1022 761 1028 2301
Fragments arranged by size:
2301 2258 1028 1022 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4662 5892 5977 7048 4662
Size: 1230 85 1071 5445
Fragments arranged by size:
5445 1230 1071 85
BsmI GAATG_Cn'
Cuts at: 2015 4356 5342 5435 2015
Size: 2341 986 93 4411
Fragments arranged by size:
4411 2341 986 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3638 3668 4662 5150 5892 5977 7048
Size: 399 1848 30 994 488 742 85 1071
Cuts at: 7048 1391
Size: 2174
Fragments arranged by size:
2174 1848 1071 994 742 488 399 85
30
BspEI T'CCGG_A
Cuts at: 7494 7494
Size: 7831
BspHI T'CATG_A
Cuts at: 5720 7450 5720
Size: 1730 6101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3053 4174 5186 844
Size: 2209 1121 1012 3489
Fragments arranged by size:
3489 2209 1121 1012
BsrBI CCG'CTC
Cuts at: 1394 1823 5718 1394
Size: 429 3895 3507
Fragments arranged by size:
3895 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 2948 3048 6326 6500 65
Size: 1183 1278 422 100 3278 174 1396
Fragments arranged by size:
3278 1396 1278 1183 422 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 4258 6475 6662 1700
Size: 110 2448 2217 187 2869
Fragments arranged by size:
2869 2448 2217 187 110
BsrGI T'GTAC_A
Cuts at: 96 2333 96
Size: 2237 5594
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7831
BssSI C'ACGA_G
Cuts at: 2385 5891 6903 2385
Size: 3506 1012 3313
Fragments arranged by size:
3506 3313 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 4487 794
Size: 1560 2133 4138
Fragments arranged by size:
4138 2133 1560
BstBI TT'CG_AA
Cuts at: 2208 6635 2208
Size: 4427 3404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7831
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 7831
BstYI r'GATC_y
Cuts at: 1972 4075 5161 5528 5913 5930 7382 7468 7480
Size: 2103 1086 367 385 17 1452 86 12
Cuts at: 7480 7826 1972
Size: 346 1977
Fragments arranged by size:
2103 1977 1452 1086 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 4461 4461
Size: 7831
Bsu36I CC'TnA_GG
Cuts at: 3321 3321
Size: 7831
BtgI C'CryG_G
Cuts at: 513 1065 2004 3370 3447 513
Size: 552 939 1366 77 4897
Fragments arranged by size:
4897 1366 939 552 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7092
Fragments arranged by size:
7092 683 56
BtsI GCAGTG_nn'
Cuts at: 2058 4231 4654 5347 6141 6161 2058
Size: 2173 423 693 794 20 3728
Fragments arranged by size:
3728 2173 794 693 423 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7796
DraI TTT'AAA
Cuts at: 4284 4891 5135 4284
Size: 607 244 6980
Fragments arranged by size:
6980 607 244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4800 5025 6838 809
Size: 507 3484 225 1813 1802
Fragments arranged by size:
3484 1813 1802 507 225
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3072 4256 5278
Size: 54 1425 50 275 18 1242 1184 1022
Cuts at: 5278 6167 7796 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1242 1184 1022 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4256 5278 1537
Size: 275 2444 1022 4090
Fragments arranged by size:
4090 2444 1022 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 2355 3344 5761 1353
Size: 894 108 989 2417 3423
Fragments arranged by size:
3423 2417 989 894 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3109 6418 6792 6938 387
Size: 1064 138 435 1085 3309 374 146 1280
Fragments arranged by size:
3309 1280 1085 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 4660 4660
Size: 7831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2286 3001 3441 4541 4950 5894
Size: 393 468 186 715 440 1100 409 944
Cuts at: 5894 6491 7278 7546 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1100 944 787 715 597 468 440
409 393 268 186
EcoO109I rG'GnC_Cy
Cuts at: 2111 2111
Size: 7831
EcoRI G'AATT_C
Cuts at: 3823 4753 5140 5518 3823
Size: 930 387 378 6136
Fragments arranged by size:
6136 930 387 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5030 5030
Size: 7831
FspI TGC'GCA
Cuts at: 6337 6337
Size: 7831
HaeII r_GCGC'y
Cuts at: 1678 5002 6978 1678
Size: 3324 1976 2531
Fragments arranged by size:
3324 2531 1976
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3281 3313 3553 3585 4487
Size: 32 135 32 1709 32 240 32 902
Cuts at: 4487 4519 6472 6504 6546 6578 1373
Size: 32 1953 32 42 32 2626
Fragments arranged by size:
2626 1953 1709 902 240 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 5175 5421 669
Size: 988 281 520 2717 246 3079
Fragments arranged by size:
3079 2717 988 520 281 246
HindIII A'AGCT_T
Cuts at: 748 4398 748
Size: 3650 4181
HpaI GTT'AAC
Cuts at: 5421 5421
Size: 7831
KasI G'GCGC_C
Cuts at: 4998 4998
Size: 7831
KpnI G_GTAC'C
Cuts at: 5156 5156
Size: 7831
MfeI C'AATT_G
Cuts at: 3285 3642 5430 3285
Size: 357 1788 5686
Fragments arranged by size:
5686 1788 357
MluI A'CGCG_T
Cuts at: 7707 7707
Size: 7831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2371 3187 3738 6945 7129 1305
Size: 1066 816 551 3207 184 2007
Fragments arranged by size:
3207 2007 1066 816 551 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3074 10
Size: 54 1425 343 1242 4767
Fragments arranged by size:
4767 1425 1242 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3372 3560 5789 6148 6307 518
Size: 648 2206 188 2229 359 159 2042
Fragments arranged by size:
2229 2206 2042 648 359 188 159
MspA1I CmG'CkG
Cuts at: 1606 2498 3671 5247 5924 7072 7317 1606
Size: 892 1173 1576 677 1148 245 2120
Fragments arranged by size:
2120 1576 1173 1148 892 677 245
NarI GG'CG_CC
Cuts at: 4999 4999
Size: 7831
NcoI C'CATG_G
Cuts at: 513 1065 2004 3370 513
Size: 552 939 1366 4974
Fragments arranged by size:
4974 1366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7831
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7831
NotI GC'GGCC_GC
Cuts at: 5278 5278
Size: 7831
NspI r_CATG'y
Cuts at: 1132 1229 3335 3478 3757 5189 6734 1132
Size: 97 2106 143 279 1432 1545 2229
Fragments arranged by size:
2229 2106 1545 1432 279 143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7831
PciI A'CATG_T
Cuts at: 3331 3753 6730 3331
Size: 422 2977 4432
Fragments arranged by size:
4432 2977 422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 3644 1108
Size: 1282 1254 5295
Fragments arranged by size:
5295 1282 1254
PfoI T'CCnGG_A
Cuts at: 3799 3799
Size: 7831
PmeI GTTT'AAAC
Cuts at: 5135 5135
Size: 7831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5900 5932 7413 7445 5900
Size: 32 1481 32 6286
Fragments arranged by size:
6286 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4964 1613
Size: 3351 4480
PsiI TTA'TAA
Cuts at: 3885 5401 3885
Size: 1516 6315
PstI C_TGCA'G
Cuts at: 830 2128 2359 4711 5183 830
Size: 1298 231 2352 472 3478
Fragments arranged by size:
3478 2352 1298 472 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7831
SacI G_AGCT'C
Cuts at: 4662 4662
Size: 7831
SalI G'TCGA_C
Cuts at: 1936 5173 1936
Size: 3237 4594
SbfI CC_TGCA'GG
Cuts at: 5183 5183
Size: 7831
ScaI AGT'ACT
Cuts at: 4098 6079 7791 4098
Size: 1981 1712 4138
Fragments arranged by size:
4138 1981 1712
SexAI A'CCwGG_T
Cuts at: 3970 3970
Size: 7831
SfcI C'TryA_G
Cuts at: 826 1045 2124 2355 3402 4108 4137 4707 5179
Size: 219 1079 231 1047 706 29 570 472
Cuts at: 5179 6314 6995 7186 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1079 1047 706 681 570 472
231 219 191 29
SfoI GGC'GCC
Cuts at: 5000 5000
Size: 7831
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7831
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7831
SmaI CCC'GGG
Cuts at: 1872 5158 1872
Size: 3286 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2272 2539 3659 5935 6836 7098
Size: 197 926 329 267 1120 2276 901 262
Cuts at: 7098 820
Size: 1553
Fragments arranged by size:
2276 1553 1120 926 901 329 267 262
197
SnaBI TAC'GTA
Cuts at: 493 5622 493
Size: 5129 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7831
SphI G_CATG'C
Cuts at: 1132 5189 1132
Size: 4057 3774
SspI AAT'ATT
Cuts at: 5 52 2562 5755 5
Size: 47 2510 3193 2081
Fragments arranged by size:
3193 2510 2081 47
StuI AGG'CCT
Cuts at: 1517 3437 1517
Size: 1920 5911
StyI C'CwwG_G
Cuts at: 513 1065 2004 3370 5270 513
Size: 552 939 1366 1900 3074
Fragments arranged by size:
3074 1900 1366 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5858 5875 6028 6213 6658 325
Size: 1255 4278 17 153 185 445 1498
Fragments arranged by size:
4278 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2333 3334 3750
Size: 274 80 33 51 1443 356 1001 416
Cuts at: 3750 4096 6077 7789 96
Size: 346 1981 1712 138
Fragments arranged by size:
1981 1712 1443 1001 416 356 346 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4011 4043 7791 7823 4011
Size: 32 3748 32 4019
Fragments arranged by size:
4019 3748 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7831
XbaI T'CTAG_A
Cuts at: 5167 5167
Size: 7831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5125 5125
Size: 7831
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7831
XmaI C'CCGG_G
Cuts at: 1870 5156 1870
Size: 3286 4545
XmnI GAAnn'nnTTC
Cuts at: 2619 3527 3555 3933 4098 4417 5013 5960 2619
Size: 908 28 378 165 319 596 947 4490
Fragments arranged by size:
4490 947 908 596 378 319 165 28
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5639 276
Size: 53 83 186 936 4105 2468
Fragments arranged by size:
4105 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SfiI SrfI SwaI