Restriction map for
pFN21AB0494
(Circular) (Six-base) MAPSORT of: pFN21AB0494.seq Check: 3118 from: 1 to: 7822
pFN21AB0494 7822 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4062 4062
Size: 7822
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2406 2991 5632 278
Size: 53 83 186 936 870 585 2641 2468
Fragments arranged by size:
2641 2468 936 870 585 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3372 5165 1937
Size: 1435 1793 4594
Fragments arranged by size:
4594 1793 1435
Acc65I G'GTAC_C
Cuts at: 5143 5143
Size: 7822
AclI AA'CG_TT
Cuts at: 1563 5949 6322 1563
Size: 4386 373 3063
Fragments arranged by size:
4386 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2593 3917 4553 4697 5885 7269 7537 1632
Size: 961 1324 636 144 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1324 1188 961 636 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7625
AflIII A'CryG_T
Cuts at: 1203 2742 4890 6721 7698 1203
Size: 1539 2148 1831 977 1327
Fragments arranged by size:
2148 1831 1539 1327 977
AgeI A'CCGG_T
Cuts at: 6653 6653
Size: 7822
AhdI GACnn_n'nnGTC
Cuts at: 6551 6551
Size: 7822
AleI CACnn'nnGTG
Cuts at: 1166 3869 1166
Size: 2703 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4319 1184
Size: 3135 4687
AlwNI CAG_nnn'CTG
Cuts at: 2926 2978 3227 3316 3341 3478 5565 7137 2926
Size: 52 249 89 25 137 2087 1572 3611
Fragments arranged by size:
3611 2087 1572 249 137 89 52 25
ApaI G_GGCC'C
Cuts at: 2231 4084 2231
Size: 1853 5969
ApaLI G'TGCA_C
Cuts at: 4049 5879 7035 4049
Size: 1830 1156 4836
Fragments arranged by size:
4836 1830 1156
ApoI r'AATT_y
Cuts at: 1493 2901 5131 5357 5509 7618 1493
Size: 1408 2230 226 152 2109 1697
Fragments arranged by size:
2230 2109 1697 1408 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6376 160
Size: 872 5344 1606
Fragments arranged by size:
5344 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7822
AvaI C'yCGr_G
Cuts at: 1870 1943 2710 4694 4705 4932 5147 1870
Size: 73 767 1984 11 227 215 4545
Fragments arranged by size:
4545 1984 767 227 215 73 11
BamHI G'GATC_C
Cuts at: 4717 5152 5519 4717
Size: 435 367 7020
Fragments arranged by size:
7020 435 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2864 3119 3365 3686
Size: 325 217 627 423 654 255 246 321
Cuts at: 3686 4064 4744 4912 5143 6598 618
Size: 378 680 168 231 1455 1842
Fragments arranged by size:
1842 1455 680 654 627 423 378 325
321 255 246 231 217 168
BanII G_rGCy'C
Cuts at: 1391 2231 2653 3448 3674 3788 3959 4084 4455
Size: 840 422 795 226 114 171 125 371
Cuts at: 4455 4695 5141 1391
Size: 240 446 4072
Fragments arranged by size:
4072 840 795 446 422 371 240 226
171 125 114
BbeI G_GCGC'C
Cuts at: 2214 4068 4748 2214
Size: 1854 680 5288
Fragments arranged by size:
5288 1854 680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 4478 928
Size: 971 575 2004 4272
Fragments arranged by size:
4272 2004 971 575
BbvCI CC'TCA_GC
Cuts at: 3293 4493 3293
Size: 1200 6622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6013 6047 1903
Size: 34 4076 34 3678
Fragments arranged by size:
4076 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 5714 6924 3383
Size: 2331 1210 4281
Fragments arranged by size:
4281 2331 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7822
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2142 4708 6433
Size: 107 122 71 631 605 470 2566 1725
Cuts at: 6433 136
Size: 1525
Fragments arranged by size:
2566 1725 1525 631 605 470 122 107
71
BglII A'GATC_T
Cuts at: 4677 7817 4677
Size: 3140 4682
BlpI GC'TnA_GC
Cuts at: 2496 3447 3782 3797 4922 5239 2496
Size: 951 335 15 1125 317 5079
Fragments arranged by size:
5079 1125 951 335 317 15
Bme1580I G_kGCm'C
Cuts at: 1790 2231 2267 2294 2869 3124 3585 3927 4053
Size: 441 36 27 575 255 461 342 126
Cuts at: 4053 4084 4917 5883 7039 1790
Size: 31 833 966 1156 2573
Fragments arranged by size:
2573 1156 966 833 575 461 441 342
255 126 36 31 27
BmgBI CAC'GTC
Cuts at: 1318 3302 4315 1318
Size: 1984 1013 4825
Fragments arranged by size:
4825 1984 1013
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2105 2384 2809
Size: 416 53 83 399 24 688 279 425
Cuts at: 2809 3240 3588 4621 5553 6511 442
Size: 431 348 1033 932 958 1753
Fragments arranged by size:
1753 1033 958 932 688 431 425 416
399 348 279 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4720 4720
Size: 7822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2070 2290 2416 2676 2755 4010 6482
Size: 827 4 220 126 260 79 1255 2472
Cuts at: 6482 1239
Size: 2579
Fragments arranged by size:
2579 2472 1255 827 260 220 126 79
4
Bpu10I CC'TnA_GC
Cuts at: 2549 3293 4085 4493 4944 2549
Size: 744 792 408 451 5427
Fragments arranged by size:
5427 792 744 451 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4586 5947 6812 7110 4586
Size: 1361 865 298 5298
Fragments arranged by size:
5298 1361 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 2681 4133 6485 882
Size: 617 816 366 1452 2352 2219
Fragments arranged by size:
2352 2219 1452 816 617 366
BsaAI yAC'GTr
Cuts at: 493 1204 2563 2743 5613 493
Size: 711 1359 180 2870 2702
Fragments arranged by size:
2870 2702 1359 711 180
BsaBI GATnn'nnATC
Cuts at: 4196 7816 4196
Size: 3620 4202
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 2381 2403 2471
Size: 53 83 186 936 678 170 22 68
Cuts at: 2471 2954 2988 4065 4745 5629 6011 6646 275
Size: 483 34 1077 680 884 382 635 1451
Fragments arranged by size:
1451 1077 936 884 680 678 635 483
382 186 170 83 68 53 34 22
BsaWI w'CCGG_w
Cuts at: 6255 6653 6927 7074 7485 6255
Size: 398 274 147 411 6592
Fragments arranged by size:
6592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2625 2655 2732 2762 4232
Size: 30 165 30 1222 30 77 30 1470
Cuts at: 4232 4262 4880 4910 4947 4977 1178
Size: 30 618 30 37 30 4023
Fragments arranged by size:
4023 1470 1222 618 165 77 37 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2471 4991 1186
Size: 1069 216 2520 4017
Fragments arranged by size:
4017 2520 1069 216
BseYI C'CCAG_C
Cuts at: 2231 2580 3115 3125 3667 3927 4162 4235 4422
Size: 349 535 10 542 260 235 73 187
Cuts at: 4422 4518 4628 4832 5017 7025 7521 2231
Size: 96 110 204 185 2008 496 2532
Fragments arranged by size:
2532 2008 542 535 496 349 260 235
204 187 185 110 96 73 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 3893 4781 1163
Size: 573 399 1758 888 4204
Fragments arranged by size:
4204 1758 888 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2388 2512 3199 5272 6033 7061
Size: 275 186 387 124 687 2073 761 1028
Cuts at: 7061 1540
Size: 2301
Fragments arranged by size:
2301 2073 1028 761 687 387 275 186
124
BsiHKAI G_wGCw'C
Cuts at: 3448 3798 4053 4443 4455 5883 5968 7039 3448
Size: 350 255 390 12 1428 85 1071 4231
Fragments arranged by size:
4231 1428 1071 390 350 255 85 12
BsmI GAATG_Cn'
Cuts at: 2968 5333 5426 2968
Size: 2365 93 5364
Fragments arranged by size:
5364 2365 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2237
Size: 7822
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2267 2294 2653 2869 3124 3448
Size: 399 441 36 27 359 216 255 324
Cuts at: 3448 3585 3674 3788 3798 3927 3959 4053 4084
Size: 137 89 114 10 129 32 94 31
Cuts at: 4084 4443 4455 4695 4917 5141 5883 5968 7039
Size: 359 12 240 222 224 742 85 1071
Cuts at: 7039 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 441 399 359 359 324
255 240 224 222 216 137 129 114
94 89 85 36 32 31 27 12
10
BspEI T'CCGG_A
Cuts at: 7485 7485
Size: 7822
BspHI T'CATG_A
Cuts at: 5711 7441 5711
Size: 1730 6092
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4062 4539 4779 5177 844
Size: 3218 477 240 398 3489
Fragments arranged by size:
3489 3218 477 398 240
BsrBI CCG'CTC
Cuts at: 1394 1823 2508 2894 3964 4921 5709 1394
Size: 429 685 386 1070 957 788 3507
Fragments arranged by size:
3507 1070 957 788 685 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 3216 4096 6317 6491 65
Size: 1183 1968 880 2221 174 1396
Fragments arranged by size:
2221 1968 1396 1183 880 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3630 3651 4318 5085 6466 6653 1700
Size: 110 1820 21 667 767 1381 187 2869
Fragments arranged by size:
2869 1820 1381 767 667 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 3438 96
Size: 3342 4480
BssHII G'CGCG_C
Cuts at: 1925 4784 1925
Size: 2859 4963
BssSI C'ACGA_G
Cuts at: 5882 6894 5882
Size: 1012 6810
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2935 3013 3227 794
Size: 2141 78 214 5389
Fragments arranged by size:
5389 2141 214 78
BstBI TT'CG_AA
Cuts at: 6626 6626
Size: 7822
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7822
BstXI CCAn_nnnn'nTGG
Cuts at: 2206 3430 4463 4998 2206
Size: 1224 1033 535 5030
Fragments arranged by size:
5030 1224 1033 535
BstYI r'GATC_y
Cuts at: 1972 2836 3703 4677 4717 5152 5519 5904 5921
Size: 864 867 974 40 435 367 385 17
Cuts at: 5921 7373 7459 7471 7817 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 974 867 864 435 385 367
346 86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 2216 2616 2817 3741 3822
Size: 552 939 181 31 400 201 924 81
Cuts at: 3822 4800 513
Size: 978 3535
Fragments arranged by size:
3535 978 939 924 552 400 201 181
81 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3160 4217 487
Size: 683 56 1934 1057 4092
Fragments arranged by size:
4092 1934 1057 683 56
BtsI GCAGTG_nn'
Cuts at: 2024 3228 3939 4769 5338 6132 6152 2024
Size: 1204 711 830 569 794 20 3694
Fragments arranged by size:
3694 1204 830 794 711 569 20
ClaI AT'CG_AT
Cuts at: 1554 3378 1554
Size: 1824 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7787
DraI TTT'AAA
Cuts at: 5126 5126
Size: 7822
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4319 6829 809
Size: 507 3003 2510 1802
Fragments arranged by size:
3003 2510 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2196 2284
Size: 54 1425 50 275 18 177 189 88
Cuts at: 2284 2509 2814 3063 3250 4044 5052 5087 5269
Size: 225 305 249 187 794 1008 35 182
Cuts at: 5269 6158 7787 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1008 889 794 305 275 249
225 189 187 182 177 88 54 50
43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2509 5269 1537
Size: 275 697 2760 4090
Fragments arranged by size:
4090 2760 697 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3503 4224 5106 5752 1353
Size: 2150 721 882 646 3423
Fragments arranged by size:
3423 2150 882 721 646
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3726 6409 6783 6929 387
Size: 1064 138 2137 2683 374 146 1280
Fragments arranged by size:
2683 2137 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3446 4453 3446
Size: 1007 6815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2070 2290 2416 2593 2676 2755
Size: 393 434 4 220 126 177 83 79
Cuts at: 2755 3917 4010 4553 4697 5885 6482 7269 7537
Size: 1162 93 543 144 1188 597 787 268
Cuts at: 7537 1239
Size: 1524
Fragments arranged by size:
1524 1188 1162 787 597 543 434 393
268 220 177 144 126 93 83 79
4
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 4058 2684
Size: 1374 6448
EcoO109I rG'GnC_Cy
Cuts at: 2069 2135 2228 3104 3242 3313 4026 4081 4427
Size: 66 93 876 138 71 713 55 346
Cuts at: 4427 4564 2069
Size: 137 5327
Fragments arranged by size:
5327 876 713 346 138 137 93 71
66 55
EcoRI G'AATT_C
Cuts at: 5131 5509 5131
Size: 378 7444
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5086 5086
Size: 7822
FspI TGC'GCA
Cuts at: 6328 6328
Size: 7822
HaeII r_GCGC'y
Cuts at: 1678 2214 3027 4068 4748 6969 1678
Size: 536 813 1041 680 2221 2531
Fragments arranged by size:
2531 2221 1041 813 680 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 2693 2725 2914
Size: 32 135 32 612 32 477 32 189
Cuts at: 2914 2946 2995 3027 3286 3318 4232 4264 6463
Size: 32 49 32 259 32 914 32 2199
Cuts at: 6463 6495 6537 6569 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2199 914 612 477 259 189 135
49 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2992 4594 5166 5412 669
Size: 988 281 1054 1602 572 246 3079
Fragments arranged by size:
3079 1602 1054 988 572 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7822
HpaI GTT'AAC
Cuts at: 5412 5412
Size: 7822
KasI G'GCGC_C
Cuts at: 2210 4064 4744 2210
Size: 1854 680 5288
Fragments arranged by size:
5288 1854 680
KpnI G_GTAC'C
Cuts at: 5147 5147
Size: 7822
MfeI C'AATT_G
Cuts at: 5421 5421
Size: 7822
MluI A'CGCG_T
Cuts at: 7698 7698
Size: 7822
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3550 4551 6936 7120 1305
Size: 2245 1001 2385 184 2007
Fragments arranged by size:
2385 2245 2007 1001 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2198 5054 10
Size: 54 1425 343 177 189 2856 2778
Fragments arranged by size:
2856 2778 1425 343 189 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2561 3221 3560 3740 3869 4446
Size: 648 1038 357 660 339 180 129 577
Cuts at: 4446 4461 5780 6139 6298 518
Size: 15 1319 359 159 2042
Fragments arranged by size:
2042 1319 1038 660 648 577 359 357
339 180 159 129 15
MspA1I CmG'CkG
Cuts at: 1606 2218 2394 3625 4258 4422 4795 4960 5238
Size: 612 176 1231 633 164 373 165 278
Cuts at: 5238 5915 7063 7308 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1231 1148 677 633 612 373 278
245 176 165 164
NaeI GCC'GGC
Cuts at: 3653 3653
Size: 7822
NarI GG'CG_CC
Cuts at: 2211 4065 4745 2211
Size: 1854 680 5288
Fragments arranged by size:
5288 1854 680
NcoI C'CATG_G
Cuts at: 513 1065 2004 2185 3741 4800 513
Size: 552 939 181 1556 1059 3535
Fragments arranged by size:
3535 1556 1059 939 552 181
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7822
NgoMIV G'CCGG_C
Cuts at: 3651 3651
Size: 7822
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7822
NotI GC'GGCC_GC
Cuts at: 2509 5269 2509
Size: 2760 5062
NruI TCG'CGA
Cuts at: 4202 4202
Size: 7822
NspI r_CATG'y
Cuts at: 1132 1229 4078 5180 6725 1132
Size: 97 2849 1102 1545 2229
Fragments arranged by size:
2849 2229 1545 1102 97
PasI CC'CwG_GG
Cuts at: 1348 2224 2887 3184 4060 4469 4558 1348
Size: 876 663 297 876 409 89 4612
Fragments arranged by size:
4612 876 876 663 409 297 89
PciI A'CATG_T
Cuts at: 6721 6721
Size: 7822
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 3115 3265 3316 3478 1108
Size: 1266 741 150 51 162 5452
Fragments arranged by size:
5452 1266 741 162 150 51
PfoI T'CCnGG_A
Cuts at: 3315 3771 3894 3987 4507 4542 3315
Size: 456 123 93 520 35 6595
Fragments arranged by size:
6595 520 456 123 93 35
PmeI GTTT'AAAC
Cuts at: 5126 5126
Size: 7822
PmlI CAC'GTG
Cuts at: 2743 2743
Size: 7822
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5891 5923 7404 7436 5891
Size: 32 1481 32 6277
Fragments arranged by size:
6277 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 3242 3313 4427 2069
Size: 1173 71 1114 5464
Fragments arranged by size:
5464 1173 1114 71
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7822
PsiI TTA'TAA
Cuts at: 5392 5392
Size: 7822
PspOMI G'GGCC_C
Cuts at: 2227 4080 2227
Size: 1853 5969
PspXI vC'TCGA_Gb
Cuts at: 4705 4705
Size: 7822
PstI C_TGCA'G
Cuts at: 830 3096 3624 3999 4263 4776 4872 5174 830
Size: 2266 528 375 264 513 96 302 3478
Fragments arranged by size:
3478 2266 528 513 375 302 264 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7822
PvuII CAG'CTG
Cuts at: 2394 3625 4258 4422 4960 2394
Size: 1231 633 164 538 5256
Fragments arranged by size:
5256 1231 633 538 164
RsrII CG'GwC_CG
Cuts at: 4321 4321
Size: 7822
SacI G_AGCT'C
Cuts at: 3448 4455 3448
Size: 1007 6815
SacII CC_GC'GG
Cuts at: 2219 2219
Size: 7822
SalI G'TCGA_C
Cuts at: 1936 5164 1936
Size: 3228 4594
SanDI GG'GwC_CC
Cuts at: 3242 4427 3242
Size: 1185 6637
SapI GCTCTTCn'nnn_
Cuts at: 3503 3503
Size: 7822
SbfI CC_TGCA'GG
Cuts at: 4776 5174 4776
Size: 398 7424
ScaI AGT'ACT
Cuts at: 2525 2786 6070 7782 2525
Size: 261 3284 1712 2565
Fragments arranged by size:
3284 2565 1712 261
SexAI A'CCwGG_T
Cuts at: 3426 3426
Size: 7822
SfcI C'TryA_G
Cuts at: 826 1045 2847 3092 3620 3995 4259 4772 4868
Size: 219 1802 245 528 375 264 513 96
Cuts at: 4868 5170 6305 6986 7177 826
Size: 302 1135 681 191 1471
Fragments arranged by size:
1802 1471 1135 681 528 513 375 302
264 245 219 191 96
SfoI GGC'GCC
Cuts at: 2212 4066 4746 2212
Size: 1854 680 5288
Fragments arranged by size:
5288 1854 680
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7822
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7822
SmaI CCC'GGG
Cuts at: 1872 5149 1872
Size: 3277 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2710 4601 4705 5926 6827 7089
Size: 197 926 767 1891 104 1221 901 262
Cuts at: 7089 820
Size: 1553
Fragments arranged by size:
1891 1553 1221 926 901 767 262 197
104
SnaBI TAC'GTA
Cuts at: 493 5613 493
Size: 5120 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7822
SphI G_CATG'C
Cuts at: 1132 5180 1132
Size: 4048 3774
SspI AAT'ATT
Cuts at: 5 52 5746 5
Size: 47 5694 2081
Fragments arranged by size:
5694 2081 47
StuI AGG'CCT
Cuts at: 1517 3464 4849 1517
Size: 1947 1385 4490
Fragments arranged by size:
4490 1947 1385
StyI C'CwwG_G
Cuts at: 513 1065 2004 2185 2368 3741 4800 4971 5261
Size: 552 939 181 183 1373 1059 171 290
Cuts at: 5261 513
Size: 3074
Fragments arranged by size:
3074 1373 1059 939 552 290 183 181
171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 2374 3069 3107 3881 4990 5000
Size: 1255 461 333 695 38 774 1109 10
Cuts at: 5000 5009 5849 5866 6019 6204 6649 325
Size: 9 840 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1109 840 774 695 461 445
333 185 153 38 17 10 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 2784 3438
Size: 274 80 33 51 1443 546 261 654
Cuts at: 3438 4751 6068 7780 96
Size: 1313 1317 1712 138
Fragments arranged by size:
1712 1443 1317 1313 654 546 274 261
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 2625 2657 2732 2764 3876 3908 4419
Size: 32 439 32 75 32 1112 32 511
Cuts at: 4419 4451 4947 4979 7782 7814 2154
Size: 32 496 32 2803 32 2162
Fragments arranged by size:
2803 2162 1112 511 496 439 75 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7822
XbaI T'CTAG_A
Cuts at: 5158 5158
Size: 7822
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3181 3566 3697 5047 3181
Size: 385 131 1350 5956
Fragments arranged by size:
5956 1350 385 131
XhoI C'TCGA_G
Cuts at: 1943 2710 4705 1943
Size: 767 1995 5060
Fragments arranged by size:
5060 1995 767
XmaI C'CCGG_G
Cuts at: 1870 5147 1870
Size: 3277 4545
XmnI GAAnn'nnTTC
Cuts at: 5951 5951
Size: 7822
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2404 2989 5630 276
Size: 53 83 186 936 870 585 2641 2468
Fragments arranged by size:
2641 2468 936 870 585 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoRV FseI FspAI NsiI PacI PsrI
SfiI SrfI SwaI