Restriction map for
pFN21AB0486
(Circular) (Six-base) MAPSORT of: pFN21AB0486.seq Check: 6841 from: 1 to: 7795
pFN21AB0486 7795 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3131 3131
Size: 7795
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5605 278
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 5138 1937
Size: 520 2681 4594
Fragments arranged by size:
4594 2681 520
Acc65I G'GTAC_C
Cuts at: 5116 5116
Size: 7795
AclI AA'CG_TT
Cuts at: 1563 5922 6295 1563
Size: 4359 373 3063
Fragments arranged by size:
4359 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2906 3365 3509 3550 4599 4799 5858
Size: 689 585 459 144 41 1049 200 1059
Cuts at: 5858 7242 7510 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1059 1049 689 585 459 268
200 144 41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7598
AflIII A'CryG_T
Cuts at: 1203 4068 6694 7671 1203
Size: 2865 2626 977 1327
Fragments arranged by size:
2865 2626 1327 977
AgeI A'CCGG_T
Cuts at: 6626 6626
Size: 7795
AhdI GACnn_n'nnGTC
Cuts at: 2453 6524 2453
Size: 4071 3724
AleI CACnn'nnGTG
Cuts at: 1166 3084 4553 4930 4964 1166
Size: 1918 1469 377 34 3997
Fragments arranged by size:
3997 1918 1469 377 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3153 3200 4571 4948 1184
Size: 1969 47 1371 377 4031
Fragments arranged by size:
4031 1969 1371 377 47
AlwNI CAG_nnn'CTG
Cuts at: 2431 2848 4324 4458 4517 5538 7110 2431
Size: 417 1476 134 59 1021 1572 3116
Fragments arranged by size:
3116 1572 1476 1021 417 134 59
ApaI G_GGCC'C
Cuts at: 2096 4383 2096
Size: 2287 5508
ApaLI G'TGCA_C
Cuts at: 2063 5852 7008 2063
Size: 3789 1156 2850
Fragments arranged by size:
3789 2850 1156
ApoI r'AATT_y
Cuts at: 1493 3619 4331 5104 5330 5482 7591 1493
Size: 2126 712 773 226 152 2109 1697
Fragments arranged by size:
2126 2109 1697 773 712 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6349 160
Size: 872 5317 1606
Fragments arranged by size:
5317 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2487 3153 4096 5120 1870
Size: 73 253 291 666 943 1024 4545
Fragments arranged by size:
4545 1024 943 666 291 253 73
AvrII C'CTAG_G
Cuts at: 2509 2509
Size: 7795
BamHI G'GATC_C
Cuts at: 2560 5125 5492 2560
Size: 2565 367 4863
Fragments arranged by size:
4863 2565 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2045 3075 3416 5116
Size: 325 217 627 248 10 1030 341 1700
Cuts at: 5116 6571 618
Size: 1455 1842
Fragments arranged by size:
1842 1700 1455 1030 627 341 325 248
217 10
BanII G_rGCy'C
Cuts at: 1391 2096 3642 3927 4013 4376 4383 5087 5114
Size: 705 1546 285 86 363 7 704 27
Cuts at: 5114 1391
Size: 4072
Fragments arranged by size:
4072 1546 705 704 363 285 86 27
7
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 7795
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2727 2729 3300 4844 928
Size: 971 828 2 571 1544 3879
Fragments arranged by size:
3879 1544 971 828 571 2
BbvCI CC'TCA_GC
Cuts at: 3014 3027 3594 4200 4805 4889 3014
Size: 13 567 606 605 84 5920
Fragments arranged by size:
5920 606 605 567 84 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 5986 6020 1903
Size: 34 1622 34 2393 34 3678
Fragments arranged by size:
3678 2393 1622 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2720 5687 6897 2720
Size: 2967 1210 3618
Fragments arranged by size:
3618 2967 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7795
BfrBI ATG'CAT
Cuts at: 4939 4939
Size: 7795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2377 6406 136
Size: 107 122 71 631 605 705 4029 1525
Fragments arranged by size:
4029 1525 705 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3427 7790 3427
Size: 4363 3432
BlpI GC'TnA_GC
Cuts at: 5212 5212
Size: 7795
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2067 2096 2203 3421 4050 4383 5856
Size: 260 17 29 107 1218 629 333 1473
Cuts at: 5856 7012 1790
Size: 1156 2573
Fragments arranged by size:
2573 1473 1218 1156 629 333 260 107
29 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5526 6484 442
Size: 416 53 83 399 24 4109 958 1753
Fragments arranged by size:
4109 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4346 1056
Size: 3290 4505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2741 2741
Size: 7795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2593 4781 6455 1239
Size: 1037 317 2188 1674 2579
Fragments arranged by size:
2579 2188 1674 1037 317
Bpu10I CC'TnA_GC
Cuts at: 3014 3027 3050 3594 4007 4200 4805 4889 5002
Size: 13 23 544 413 193 605 84 113
Cuts at: 5002 3014
Size: 5807
Fragments arranged by size:
5807 605 544 413 193 113 84 23
13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5920 6785 7083 5920
Size: 865 298 6632
Fragments arranged by size:
6632 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6458 882
Size: 617 4959 2219
Fragments arranged by size:
4959 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4858 5586 493
Size: 711 3654 728 2702
Fragments arranged by size:
3654 2702 728 711
BsaBI GATnn'nnATC
Cuts at: 7789 7789
Size: 7795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 5602 5984 6619
Size: 53 83 186 936 503 3566 382 635
Cuts at: 6619 275
Size: 1451
Fragments arranged by size:
3566 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3091 4914 6228 6626 6900 7047 7458 3091
Size: 1823 1314 398 274 147 411 3428
Fragments arranged by size:
3428 1823 1314 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 2630 2660 4754
Size: 30 165 30 1188 30 9 30 2094
Cuts at: 4754 4784 1178
Size: 30 4189
Fragments arranged by size:
4189 2094 1188 165 30 30 30 30
30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 3611 4117 4286 1186
Size: 1468 957 506 169 4695
Fragments arranged by size:
4695 1468 957 506 169
BseYI C'CCAG_C
Cuts at: 2900 3368 3375 3420 3701 6998 7494 2900
Size: 468 7 45 281 3297 496 3201
Fragments arranged by size:
3297 3201 496 468 281 45 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3920 4531 4811 1163
Size: 573 2184 611 280 4147
Fragments arranged by size:
4147 2184 611 573 280
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5245 6006 7034 1540
Size: 275 186 3244 761 1028 2301
Fragments arranged by size:
3244 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 3341 4437 5856 5941 7012 2067
Size: 1274 1096 1419 85 1071 2850
Fragments arranged by size:
2850 1419 1274 1096 1071 85
BsmI GAATG_Cn'
Cuts at: 2857 5306 5399 2857
Size: 2449 93 5253
Fragments arranged by size:
5253 2449 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2067 2096 2203 3341 3421 3642
Size: 399 260 17 29 107 1138 80 221
Cuts at: 3642 3927 4013 4050 4376 4383 4437 5087 5114
Size: 285 86 37 326 7 54 650 27
Cuts at: 5114 5856 5941 7012 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1138 1071 742 650 399 326 285
260 221 107 86 85 80 54 37
29 27 17 7
BspEI T'CCGG_A
Cuts at: 4914 7458 4914
Size: 2544 5251
BspHI T'CATG_A
Cuts at: 4845 5684 7414 4845
Size: 839 1730 5226
Fragments arranged by size:
5226 1730 839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3131 5150 844
Size: 2287 2019 3489
Fragments arranged by size:
3489 2287 2019
BsrBI CCG'CTC
Cuts at: 1394 1823 2950 5682 1394
Size: 429 1127 2732 3507
Fragments arranged by size:
3507 2732 1127 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3184 6290 6464 65
Size: 1183 1936 3106 174 1396
Fragments arranged by size:
3106 1936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 6439 6626 1700
Size: 110 485 4144 187 2869
Fragments arranged by size:
4144 2869 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 2422 3471 96
Size: 2326 1049 4420
Fragments arranged by size:
4420 2326 1049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7795
BssSI C'ACGA_G
Cuts at: 2060 2202 4563 5855 6867 2060
Size: 142 2361 1292 1012 2988
Fragments arranged by size:
2988 2361 1292 1012 142
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4936 794
Size: 4142 3653
BstBI TT'CG_AA
Cuts at: 6599 6599
Size: 7795
BstEII G'GTnAC_C
Cuts at: 1184 3464 1184
Size: 2280 5515
BstXI CCAn_nnnn'nTGG
Cuts at: 5014 5014
Size: 7795
BstYI r'GATC_y
Cuts at: 1972 2560 3427 5125 5492 5877 5894 7346 7432
Size: 588 867 1698 367 385 17 1452 86
Cuts at: 7432 7444 7790 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1698 1452 867 588 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4129 4129
Size: 7795
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2704 3972 4905 513
Size: 552 939 12 688 1268 933 3403
Fragments arranged by size:
3403 1268 939 933 688 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7056
Fragments arranged by size:
7056 683 56
BtsI GCAGTG_nn'
Cuts at: 3465 3719 3990 4151 4925 4937 5055 5311 6105
Size: 254 271 161 774 12 118 256 794
Cuts at: 6105 6125 3465
Size: 20 5135
Fragments arranged by size:
5135 794 774 271 256 254 161 118
20 12
ClaI AT'CG_AT
Cuts at: 1554 2676 1554
Size: 1122 6673
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7760
DraI TTT'AAA
Cuts at: 5099 5099
Size: 7795
DraIII CAC_nnn'GTG
Cuts at: 4045 4045
Size: 7795
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4868 6802 809
Size: 507 3552 1934 1802
Fragments arranged by size:
3552 1934 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 4570 5242
Size: 54 1425 50 275 18 177 2563 672
Cuts at: 5242 6131 7760 8
Size: 889 1629 43
Fragments arranged by size:
2563 1629 1425 889 672 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5242 1537
Size: 275 3430 4090
Fragments arranged by size:
4090 3430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 4058 4410 4827 5021 5725 1353
Size: 1137 1568 352 417 194 704 3423
Fragments arranged by size:
3423 1568 1137 704 417 352 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3250 4586 6382 6756 6902 387
Size: 1064 138 1661 1336 1796 374 146 1280
Fragments arranged by size:
1796 1661 1336 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2321 2593 2906 3365 3509 3550
Size: 393 644 45 272 313 459 144 41
Cuts at: 3550 4599 4781 4799 5858 6455 7242 7510 1239
Size: 1049 182 18 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 1049 787 644 597 459 393
313 272 268 182 144 45 41 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2435 3491 2258
Size: 177 1056 6562
Fragments arranged by size:
6562 1056 177
EcoO109I rG'GnC_Cy
Cuts at: 2056 2092 2093 2166 2627 2965 3031 3838 4380
Size: 36 1 73 461 338 66 807 542
Cuts at: 4380 4426 4765 2056
Size: 46 339 5086
Fragments arranged by size:
5086 807 542 461 339 338 73 66
46 36 1
EcoRI G'AATT_C
Cuts at: 4331 5104 5482 4331
Size: 773 378 6644
Fragments arranged by size:
6644 773 378
FspI TGC'GCA
Cuts at: 5039 6301 5039
Size: 1262 6533
HaeII r_GCGC'y
Cuts at: 1678 2039 6942 1678
Size: 361 4903 2531
Fragments arranged by size:
4903 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2439 2471 2591
Size: 32 135 32 536 32 299 32 120
Cuts at: 2591 2623 3419 3451 3709 3741 4112 4144 4511
Size: 32 796 32 258 32 371 32 367
Cuts at: 4511 4543 4754 4786 6436 6468 6510 6542 1373
Size: 32 211 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 796 536 371 367 299 258
211 135 120 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5139 5385 669
Size: 988 281 3201 246 3079
Fragments arranged by size:
3201 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7795
HpaI GTT'AAC
Cuts at: 5385 5385
Size: 7795
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 7795
KpnI G_GTAC'C
Cuts at: 5120 5120
Size: 7795
MfeI C'AATT_G
Cuts at: 5394 5394
Size: 7795
MluI A'CGCG_T
Cuts at: 7671 7671
Size: 7795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2811 6909 7093 1305
Size: 1506 4098 184 2007
Fragments arranged by size:
4098 2007 1506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4572 10
Size: 54 1425 343 2740 3233
Fragments arranged by size:
3233 2740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3084 4553 4930 4964 5753 6112
Size: 648 1374 544 1469 377 34 789 359
Cuts at: 6112 6271 518
Size: 159 2042
Fragments arranged by size:
2042 1469 1374 789 648 544 377 359
159 34
MspA1I CmG'CkG
Cuts at: 1606 2012 2018 2099 3137 3232 3334 3686 4204
Size: 406 6 81 1038 95 102 352 518
Cuts at: 4204 4251 4271 4324 4517 4603 4809 5211 5888
Size: 47 20 53 193 86 206 402 677
Cuts at: 5888 7036 7281 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1038 677 518 406 402 352
245 206 193 102 95 86 81 53
47 20 6
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 7795
NcoI C'CATG_G
Cuts at: 513 1065 2004 2704 3972 4905 513
Size: 552 939 700 1268 933 3403
Fragments arranged by size:
3403 1268 939 933 700 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7795
NheI G'CTAG_C
Cuts at: 1052 4342 1052
Size: 3290 4505
NotI GC'GGCC_GC
Cuts at: 5242 5242
Size: 7795
NsiI A_TGCA'T
Cuts at: 4941 4941
Size: 7795
NspI r_CATG'y
Cuts at: 1132 1229 2423 4939 4943 5153 6698 1132
Size: 97 1194 2516 4 210 1545 2229
Fragments arranged by size:
2516 2229 1545 1194 210 97 4
PasI CC'CwG_GG
Cuts at: 1348 2375 1348
Size: 1027 6768
PciI A'CATG_T
Cuts at: 6694 6694
Size: 7795
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2545 2907 1108
Size: 1437 362 5996
Fragments arranged by size:
5996 1437 362
PfoI T'CCnGG_A
Cuts at: 2253 2409 4116 2253
Size: 156 1707 5932
Fragments arranged by size:
5932 1707 156
PmeI GTTT'AAAC
Cuts at: 5099 5099
Size: 7795
PmlI CAC'GTG
Cuts at: 4858 4858
Size: 7795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 5864 5896 7377 7409 4877
Size: 32 955 32 1481 32 5263
Fragments arranged by size:
5263 1481 955 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2627 3031 3838 2166
Size: 461 404 807 6123
Fragments arranged by size:
6123 807 461 404
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7795
PsiI TTA'TAA
Cuts at: 5365 5365
Size: 7795
PspOMI G'GGCC_C
Cuts at: 2092 4379 2092
Size: 2287 5508
PstI C_TGCA'G
Cuts at: 830 2116 2337 3074 3142 3276 3738 4315 4932
Size: 1286 221 737 68 134 462 577 617
Cuts at: 4932 5147 830
Size: 215 3478
Fragments arranged by size:
3478 1286 737 617 577 462 221 215
134 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7795
PvuII CAG'CTG
Cuts at: 3137 3334 3686 4204 4251 4324 4517 4809 3137
Size: 197 352 518 47 73 193 292 6123
Fragments arranged by size:
6123 518 352 292 197 193 73 47
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 7795
SalI G'TCGA_C
Cuts at: 1936 5137 1936
Size: 3201 4594
SanDI GG'GwC_CC
Cuts at: 2627 2627
Size: 7795
SapI GCTCTTCn'nnn_
Cuts at: 2490 2490
Size: 7795
SbfI CC_TGCA'GG
Cuts at: 2337 4315 5147 2337
Size: 1978 832 4985
Fragments arranged by size:
4985 1978 832
ScaI AGT'ACT
Cuts at: 3945 6043 7755 3945
Size: 2098 1712 3985
Fragments arranged by size:
3985 2098 1712
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 7795
SfcI C'TryA_G
Cuts at: 826 1045 2112 2333 2617 2840 3062 3070 3138
Size: 219 1067 221 284 223 222 8 68
Cuts at: 3138 3272 3734 4311 4337 4928 5143 6278 6959
Size: 134 462 577 26 591 215 1135 681
Cuts at: 6959 7150 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1067 681 591 577 462 284
223 222 221 219 215 191 134 68
26 8
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 7795
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7795
SmaI CCC'GGG
Cuts at: 1872 2489 3155 5122 1872
Size: 617 666 1967 4545
Fragments arranged by size:
4545 1967 666 617
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5899 6800 7062 820
Size: 197 926 3956 901 262 1553
Fragments arranged by size:
3956 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5586 493
Size: 5093 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7795
SphI G_CATG'C
Cuts at: 1132 4939 4943 5153 1132
Size: 3807 4 210 3774
Fragments arranged by size:
3807 3774 210 4
SrfI GCCC'GGGC
Cuts at: 3155 3155
Size: 7795
SspI AAT'ATT
Cuts at: 5 52 5719 5
Size: 47 5667 2081
Fragments arranged by size:
5667 2081 47
StuI AGG'CCT
Cuts at: 1517 3310 3905 4397 1517
Size: 1793 595 492 4915
Fragments arranged by size:
4915 1793 595 492
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2509 2704 3891 3972 4905
Size: 552 939 157 348 195 1187 81 933
Cuts at: 4905 5234 513
Size: 329 3074
Fragments arranged by size:
3074 1187 939 933 552 348 329 195
157 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2033 2460 3389 3404 3659 5822 5839
Size: 1255 453 427 929 15 255 2163 17
Cuts at: 5839 5992 6177 6622 325
Size: 153 185 445 1498
Fragments arranged by size:
2163 1498 1255 929 453 445 427 255
185 153 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 3181 3471
Size: 274 80 33 51 1443 445 759 290
Cuts at: 3471 3943 4696 4720 6041 7753 96
Size: 472 753 24 1321 1712 138
Fragments arranged by size:
1712 1443 1321 759 753 472 445 290
274 138 80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 2619 2651 3320 3352 7755 7787 2193
Size: 32 394 32 669 32 4403 32 2201
Fragments arranged by size:
4403 2201 669 394 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2072 3167 1316
Size: 756 1095 5944
Fragments arranged by size:
5944 1095 756
XbaI T'CTAG_A
Cuts at: 2173 5131 2173
Size: 2958 4837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 7795
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7795
XmaI C'CCGG_G
Cuts at: 1870 2487 3153 5120 1870
Size: 617 666 1967 4545
Fragments arranged by size:
4545 1967 666 617
XmnI GAAnn'nnTTC
Cuts at: 2891 3320 5924 2891
Size: 429 2604 4762
Fragments arranged by size:
4762 2604 429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5603 276
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PspXI PsrI RsrII SacI SfiI SwaI