Restriction map for
pFN21AB0483
(Circular) (Six-base) MAPSORT of: pFN21AB0483.seq Check: 3167 from: 1 to: 6283
pFN21AB0483 6283 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2466 4346 5730 5998 1632
Size: 521 313 1880 1384 268 1917
Fragments arranged by size:
1917 1880 1384 521 313 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 2185 2996 5012 2185
Size: 811 2016 3456
Fragments arranged by size:
3456 2016 811
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2208 2240 2853 2885 2208
Size: 32 613 32 5606
Fragments arranged by size:
5606 613 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4026 5598 4026
Size: 1572 4711
ApaI G_GGCC'C
Cuts at: 3426 3426
Size: 6283
ApaLI G'TGCA_C
Cuts at: 2156 4340 5496 2156
Size: 2184 1156 2943
Fragments arranged by size:
2943 2184 1156
ApoI r'AATT_y
Cuts at: 1493 3253 3592 3818 3970 6079 1493
Size: 1760 339 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 339 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2826 3381 4837 160
Size: 872 1794 555 1456 1606
Fragments arranged by size:
1794 1606 1456 872 555
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 3308 3449 3608 1870
Size: 73 1365 141 159 4545
Fragments arranged by size:
4545 1365 159 141 73
AvrII C'CTAG_G
Cuts at: 3544 3544
Size: 6283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2066 2099 2066
Size: 33 6250
BamHI G'GATC_C
Cuts at: 3613 3980 3613
Size: 367 5916
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3132 3604 5059 618
Size: 325 217 627 1345 472 1455 1842
Fragments arranged by size:
1842 1455 1345 627 472 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2887 3426 3602 1391
Size: 705 791 539 176 4072
Fragments arranged by size:
4072 791 705 539 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 3340 928
Size: 971 282 1159 3871
Fragments arranged by size:
3871 1159 971 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4175 5385 4175
Size: 1210 5073
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BfrBI ATG'CAT
Cuts at: 2432 2432
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4894 136
Size: 107 122 71 631 605 3222 1525
Fragments arranged by size:
3222 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2466 6278 2466
Size: 3812 2471
BlpI GC'TnA_GC
Cuts at: 2047 3700 2047
Size: 1653 4630
Bme1580I G_kGCm'C
Cuts at: 1790 2160 3426 4344 5500 1790
Size: 370 1266 918 1156 2573
Fragments arranged by size:
2573 1266 1156 918 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 3376 4014
Size: 416 53 83 399 24 610 1349 638
Cuts at: 4014 4972 442
Size: 958 1753
Fragments arranged by size:
1753 1349 958 638 610 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2837 3215 3293 4943 1239
Size: 912 686 378 78 1650 2579
Fragments arranged by size:
2579 1650 912 686 378 78
Bpu10I CC'TnA_GC
Cuts at: 2487 2487
Size: 6283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4408 5273 5571 4408
Size: 865 298 5120
Fragments arranged by size:
5120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4946 882
Size: 617 3447 2219
Fragments arranged by size:
3447 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4716 5114 5388 5535 5946 4716
Size: 398 274 147 411 5053
Fragments arranged by size:
5053 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2129 2159 2240 2270 2374
Size: 30 165 30 726 30 81 30 104
Cuts at: 2374 2404 1178
Size: 30 5057
Fragments arranged by size:
5057 726 165 104 81 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2137 2395 2502 2720 1186
Size: 951 258 107 218 4749
Fragments arranged by size:
4749 951 258 218 107
BseYI C'CCAG_C
Cuts at: 2126 5486 5982 2126
Size: 3360 496 2427
Fragments arranged by size:
3360 2427 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5710
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 4344 4429 5500 2160
Size: 2184 85 1071 2943
Fragments arranged by size:
2943 2184 1071 85
BsmI GAATG_Cn'
Cuts at: 2503 2929 3794 3887 2503
Size: 426 865 93 4899
Fragments arranged by size:
4899 865 426 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2160 2887 3426 3602 4344 4429
Size: 399 306 64 727 539 176 742 85
Cuts at: 4429 5500 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 727 539 399 306 176
85 64
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 2370 4172 5902 2370
Size: 1802 1730 2751
Fragments arranged by size:
2751 1802 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2934 3638 844
Size: 2090 704 3489
Fragments arranged by size:
3489 2090 704
BsrBI CCG'CTC
Cuts at: 1394 1823 2728 4170 1394
Size: 429 905 1442 3507
Fragments arranged by size:
3507 1442 905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4778 4952 65
Size: 1183 3530 174 1396
Fragments arranged by size:
3530 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 4927 5114 1700
Size: 110 331 2786 187 2869
Fragments arranged by size:
2869 2786 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6283
BstXI CCAn_nnnn'nTGG
Cuts at: 2917 2917
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2466 3613 3980 4365 4382 5834 5920 5932
Size: 494 1147 367 385 17 1452 86 12
Cuts at: 5932 6278 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1147 494 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2244 2478 3087 2244
Size: 234 609 5440
Fragments arranged by size:
5440 609 234
BtgI C'CryG_G
Cuts at: 513 1065 2004 2151 2879 3567 513
Size: 552 939 147 728 688 3229
Fragments arranged by size:
3229 939 728 688 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 3799 4593 4613 3799
Size: 794 20 5469
Fragments arranged by size:
5469 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248
Size: 54 1425 50 275 18 1900 889 1629
Cuts at: 6248 8
Size: 43
Fragments arranged by size:
1900 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3456 4213 1353
Size: 2103 757 3423
Fragments arranged by size:
3423 2103 757
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2153 2466 2837 3215 3293 4346
Size: 393 519 2 313 371 378 78 1053
Cuts at: 4346 4943 5730 5998 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1053 787 597 519 393 378 371
313 268 78 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3272 3287 3272
Size: 15 6268
EcoO109I rG'GnC_Cy
Cuts at: 3277 3277
Size: 6283
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3340 3340
Size: 6283
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 2171 2203 2240
Size: 32 135 32 447 32 120 32 37
Cuts at: 2240 2272 2374 2406 2982 3014 3325 3357 4924
Size: 32 102 32 576 32 311 32 1567
Cuts at: 4924 4956 4998 5030 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1567 576 447 311 135 120 102
42 37 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3627 3873 669
Size: 988 281 1689 246 3079
Fragments arranged by size:
3079 1689 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 5397 5581 1305
Size: 1398 2694 184 2007
Fragments arranged by size:
2694 2007 1398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4461
Fragments arranged by size:
4461 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2263 4241 4600 4759 518
Size: 648 1097 1978 359 159 2042
Fragments arranged by size:
2042 1978 1097 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3197 3235 3699 4376 5524 5769 1606
Size: 1591 38 464 677 1148 245 2120
Fragments arranged by size:
2120 1591 1148 677 464 245 38
NcoI C'CATG_G
Cuts at: 513 1065 2004 2151 513
Size: 552 939 147 4645
Fragments arranged by size:
4645 939 552 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NsiI A_TGCA'T
Cuts at: 2434 2434
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2208 2222 2436 3641 5186 1132
Size: 97 979 14 214 1205 1545 2229
Fragments arranged by size:
2229 1545 1205 979 214 97 14
PacI TTA_AT'TAA
Cuts at: 3363 3363
Size: 6283
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6283
PfoI T'CCnGG_A
Cuts at: 2871 3270 2871
Size: 399 5884
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PspOMI G'GGCC_C
Cuts at: 3422 3422
Size: 6283
PstI C_TGCA'G
Cuts at: 830 3288 3635 830
Size: 2458 347 3478
Fragments arranged by size:
3478 2458 347
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 3197 3235 3197
Size: 38 6245
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SapI GCTCTTCn'nnn_
Cuts at: 3456 3456
Size: 6283
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 3284 3631 4766 5447 5638 826
Size: 219 2239 347 1135 681 191 1471
Fragments arranged by size:
2239 1471 1135 681 347 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3610 1872
Size: 1738 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4387 5288 5550 820
Size: 197 926 2444 901 262 1553
Fragments arranged by size:
2444 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 2436 3641 1132
Size: 1304 1205 3774
Fragments arranged by size:
3774 1304 1205
SspI AAT'ATT
Cuts at: 5 52 2971 3247 4207 5
Size: 47 2919 276 960 2081
Fragments arranged by size:
2919 2081 960 276 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6283
StyI C'CwwG_G
Cuts at: 513 1065 2004 2151 2542 2916 3315 3544 3722
Size: 552 939 147 391 374 399 229 178
Cuts at: 3722 513
Size: 3074
Fragments arranged by size:
3074 939 552 399 391 374 229 178
147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2201 2271 4310 4327 4480 4665 5110
Size: 1255 621 70 2039 17 153 185 445
Cuts at: 5110 325
Size: 1498
Fragments arranged by size:
2039 1498 1255 621 445 185 153 70
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 2805 4529
Size: 274 80 33 51 1443 159 669 1724
Cuts at: 4529 6241 96
Size: 1712 138
Fragments arranged by size:
1724 1712 1443 669 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3116 3148 6243 6275 3116
Size: 32 3095 32 3124
Fragments arranged by size:
3124 3095 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3500 1316
Size: 2184 4099
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2343 2343
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 3608 1870
Size: 1738 4545
XmnI GAAnn'nnTTC
Cuts at: 2027 4412 2027
Size: 2385 3898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbeI BbvCI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI