Restriction map for
pFN21AB0482
(Circular) (Six-base) MAPSORT of: pFN21AB0482.seq Check: 4619 from: 1 to: 7777
pFN21AB0482 7777 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3386 5587 278
Size: 53 83 186 936 1850 2201 2468
Fragments arranged by size:
2468 2201 1850 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5120 1937
Size: 3183 4594
Acc65I G'GTAC_C
Cuts at: 5098 5098
Size: 7777
AclI AA'CG_TT
Cuts at: 1563 5904 6277 1563
Size: 4341 373 3063
Fragments arranged by size:
4341 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 2735 2979 4377 4394 5840 7224 7492
Size: 839 264 244 1398 17 1446 1384 268
Cuts at: 7492 1632
Size: 1917
Fragments arranged by size:
1917 1446 1398 1384 839 268 264 244
17
AflII C'TTAA_G
Cuts at: 820 1017 2222 4895 820
Size: 197 1205 2673 3702
Fragments arranged by size:
3702 2673 1205 197
AflIII A'CryG_T
Cuts at: 1203 6676 7653 1203
Size: 5473 977 1327
Fragments arranged by size:
5473 1327 977
AgeI A'CCGG_T
Cuts at: 6608 6608
Size: 7777
AhdI GACnn_n'nnGTC
Cuts at: 6506 6506
Size: 7777
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2169 2565 2892 1184
Size: 985 396 327 6069
Fragments arranged by size:
6069 985 396 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 7745
AlwNI CAG_nnn'CTG
Cuts at: 2414 4134 5520 7092 2414
Size: 1720 1386 1572 3099
Fragments arranged by size:
3099 1720 1572 1386
ApaI G_GGCC'C
Cuts at: 2629 2629
Size: 7777
ApaLI G'TGCA_C
Cuts at: 5834 6990 5834
Size: 1156 6621
ApoI r'AATT_y
Cuts at: 1493 2008 2597 2759 2976 3323 3636 3725 3977
Size: 515 589 162 217 347 313 89 252
Cuts at: 3977 4476 4779 4831 4931 5086 5312 5464 7573
Size: 499 303 52 100 155 226 152 2109
Cuts at: 7573 1493
Size: 1697
Fragments arranged by size:
2109 1697 589 515 499 347 313 303
252 226 217 162 155 152 100 89
52
AseI AT'TA_AT
Cuts at: 160 1032 6331 160
Size: 872 5299 1606
Fragments arranged by size:
5299 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
AvaI C'yCGr_G
Cuts at: 1870 1943 5102 1870
Size: 73 3159 4545
Fragments arranged by size:
4545 3159 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3472 3505 3472
Size: 33 7744
BamHI G'GATC_C
Cuts at: 5107 5474 5107
Size: 367 7410
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2876 5098 6553 618
Size: 325 217 627 1089 2222 1455 1842
Fragments arranged by size:
2222 1842 1455 1089 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 2495 2567 2629 3282 5096 1391
Size: 644 460 72 62 653 1814 4072
Fragments arranged by size:
4072 1814 653 644 460 72 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2596 2916 3017 3370 3897 928
Size: 971 697 320 101 353 527 4808
Fragments arranged by size:
4808 971 697 527 353 320 101
BbvCI CC'TCA_GC
Cuts at: 2889 4127 2889
Size: 1238 6539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5968 6002 1903
Size: 34 4031 34 3678
Fragments arranged by size:
4031 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5669 6879 5669
Size: 1210 6567
BclI T'GATC_A
Cuts at: 1548 2656 2943 1548
Size: 1108 287 6382
Fragments arranged by size:
6382 1108 287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3512 6388 136
Size: 107 122 71 631 605 1840 2876 1525
Fragments arranged by size:
2876 1840 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7772 7772
Size: 7777
BlpI GC'TnA_GC
Cuts at: 5194 5194
Size: 7777
Bme1580I G_kGCm'C
Cuts at: 1790 2629 2881 3829 5838 6994 1790
Size: 839 252 948 2009 1156 2573
Fragments arranged by size:
2573 2009 1156 948 839 252
BmgBI CAC'GTC
Cuts at: 1318 4678 1318
Size: 3360 4417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 5508 6466
Size: 416 53 83 399 24 1868 2223 958
Cuts at: 6466 442
Size: 1753
Fragments arranged by size:
2223 1868 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 7777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3218 3610 4010 4774 6437 1239
Size: 1979 392 400 764 1663 2579
Fragments arranged by size:
2579 1979 1663 764 400 392
Bpu10I CC'TnA_GC
Cuts at: 2310 2889 3716 4127 4691 2310
Size: 579 827 411 564 5396
Fragments arranged by size:
5396 827 579 564 411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3016 4246 5902 6767 7065 3016
Size: 1230 1656 865 298 3728
Fragments arranged by size:
3728 1656 1230 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3180 6440 882
Size: 617 1681 3260 2219
Fragments arranged by size:
3260 2219 1681 617
BsaAI yAC'GTr
Cuts at: 493 1204 5568 493
Size: 711 4364 2702
Fragments arranged by size:
4364 2702 711
BsaBI GATnn'nnATC
Cuts at: 5034 7771 5034
Size: 2737 5040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3383 5584 5966 6601
Size: 53 83 186 936 1850 2201 382 635
Cuts at: 6601 275
Size: 1451
Fragments arranged by size:
2201 1850 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2567 3477 6210 6608 6882 7029 7440 2567
Size: 910 2733 398 274 147 411 2904
Fragments arranged by size:
2904 2733 910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 1178
Size: 30 165 30 1788 30 5734
Fragments arranged by size:
5734 1788 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3272 3413 4730 1186
Size: 2086 141 1317 4233
Fragments arranged by size:
4233 2086 1317 141
BseYI C'CCAG_C
Cuts at: 3938 6980 7476 3938
Size: 3042 496 4239
Fragments arranged by size:
4239 3042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5227 5988 7016 1540
Size: 275 186 3226 761 1028 2301
Fragments arranged by size:
3226 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5838 5923 6994 5838
Size: 85 1071 6621
Fragments arranged by size:
6621 1071 85
BsmI GAATG_Cn'
Cuts at: 4557 5288 5381 4557
Size: 731 93 6953
Fragments arranged by size:
6953 731 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2621 3390 2621
Size: 769 7008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2495 2567 2629 2881 3282 3829
Size: 399 245 460 72 62 252 401 547
Cuts at: 3829 5096 5838 5923 6994 1391
Size: 1267 742 85 1071 2174
Fragments arranged by size:
2174 1267 1071 742 547 460 401 399
252 245 85 72 62
BspEI T'CCGG_A
Cuts at: 2567 7440 2567
Size: 4873 2904
BspHI T'CATG_A
Cuts at: 3006 3388 4234 4609 5666 7396 3006
Size: 382 846 375 1057 1730 3387
Fragments arranged by size:
3387 1730 1057 846 382 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5132 844
Size: 4288 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5664 1394
Size: 429 3841 3507
Fragments arranged by size:
3841 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6272 6446 65
Size: 1183 5024 174 1396
Fragments arranged by size:
5024 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6421 6608 1700
Size: 110 4611 187 2869
Fragments arranged by size:
4611 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7777
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7777
BssSI C'ACGA_G
Cuts at: 5837 6849 5837
Size: 1012 6765
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7777
BstBI TT'CG_AA
Cuts at: 3228 6581 3228
Size: 3353 4424
BstEII G'GTnAC_C
Cuts at: 1184 3403 1184
Size: 2219 5558
BstYI r'GATC_y
Cuts at: 1972 5107 5474 5859 5876 7328 7414 7426 7772
Size: 3135 367 385 17 1452 86 12 346
Cuts at: 7772 1972
Size: 1977
Fragments arranged by size:
3135 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2855 4073 2855
Size: 1218 6559
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3556 487
Size: 683 56 2330 4708
Fragments arranged by size:
4708 2330 683 56
BtsI GCAGTG_nn'
Cuts at: 3543 4518 4861 5293 6087 6107 3543
Size: 975 343 432 794 20 5213
Fragments arranged by size:
5213 975 794 432 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7777
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7742
DraI TTT'AAA
Cuts at: 2319 5062 5081 2319
Size: 2743 19 5015
Fragments arranged by size:
5015 2743 19
DraIII CAC_nnn'GTG
Cuts at: 2449 2850 2449
Size: 401 7376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4405 6784 809
Size: 507 3089 2379 1802
Fragments arranged by size:
3089 2379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2703 5224 6113
Size: 54 1425 50 275 18 873 2521 889
Cuts at: 6113 7742 8
Size: 1629 43
Fragments arranged by size:
2521 1629 1425 889 873 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5224 1537
Size: 275 3412 4090
Fragments arranged by size:
4090 3412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 3909 4611 5707 1353
Size: 1137 1419 702 1096 3423
Fragments arranged by size:
3423 1419 1137 1096 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6364 6738 6884 387
Size: 1064 138 4775 374 146 1280
Fragments arranged by size:
4775 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2471 2735 2979 3218 3610 4010 4377
Size: 393 839 264 244 239 392 400 367
Cuts at: 4377 4394 4774 5840 6437 7224 7492 1239
Size: 17 380 1066 597 787 268 1524
Fragments arranged by size:
1524 1066 839 787 597 400 393 392
380 367 268 264 244 239 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2798 2477
Size: 321 7456
EcoO109I rG'GnC_Cy
Cuts at: 2625 3919 4720 2625
Size: 1294 801 5682
Fragments arranged by size:
5682 1294 801
EcoRI G'AATT_C
Cuts at: 2759 3977 5086 5464 2759
Size: 1218 1109 378 5072
Fragments arranged by size:
5072 1218 1109 378
EcoRV GAT'ATC
Cuts at: 4033 4040 4033
Size: 7 7770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5024 5024
Size: 7777
FspI TGC'GCA
Cuts at: 6283 6283
Size: 7777
HaeII r_GCGC'y
Cuts at: 1678 2919 6924 1678
Size: 1241 4005 2531
Fragments arranged by size:
4005 2531 1241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3351 3383 3440 3472 3555
Size: 32 135 32 1779 32 57 32 83
Cuts at: 3555 3587 3686 3718 6418 6450 6492 6524 1373
Size: 32 99 32 2700 32 42 32 2626
Fragments arranged by size:
2700 2626 1779 135 99 83 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4986 5121 5367 669
Size: 988 281 3048 135 246 3079
Fragments arranged by size:
3079 3048 988 281 246 135
HindIII A'AGCT_T
Cuts at: 748 3732 3751 4736 748
Size: 2984 19 985 3789
Fragments arranged by size:
3789 2984 985 19
HpaI GTT'AAC
Cuts at: 4986 5367 4986
Size: 381 7396
KpnI G_GTAC'C
Cuts at: 5102 5102
Size: 7777
MfeI C'AATT_G
Cuts at: 3814 5376 3814
Size: 1562 6215
MluI A'CGCG_T
Cuts at: 7653 7653
Size: 7777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6891 7075 1305
Size: 5586 184 2007
Fragments arranged by size:
5586 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 10
Size: 54 1425 343 873 5082
Fragments arranged by size:
5082 1425 873 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2705 3141 4325 5735 6094 6253
Size: 648 1005 534 436 1184 1410 359 159
Cuts at: 6253 518
Size: 2042
Fragments arranged by size:
2042 1410 1184 1005 648 534 436 359
159
MspA1I CmG'CkG
Cuts at: 1606 2458 5193 5870 7018 7263 1606
Size: 852 2735 677 1148 245 2120
Fragments arranged by size:
2735 2120 1148 852 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7225
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7777
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7777
NotI GC'GGCC_GC
Cuts at: 5224 5224
Size: 7777
NruI TCG'CGA
Cuts at: 2109 2109
Size: 7777
NspI r_CATG'y
Cuts at: 1132 1229 5135 6680 1132
Size: 97 3906 1545 2229
Fragments arranged by size:
3906 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2629 4665 1348
Size: 1281 2036 4460
Fragments arranged by size:
4460 2036 1281
PciI A'CATG_T
Cuts at: 6676 6676
Size: 7777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2559 1108
Size: 1451 6326
PfoI T'CCnGG_A
Cuts at: 2664 3342 4002 2664
Size: 678 660 6439
Fragments arranged by size:
6439 678 660
PmeI GTTT'AAAC
Cuts at: 5081 5081
Size: 7777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 5846 5878 7359 7391 3191
Size: 32 2623 32 1481 32 3577
Fragments arranged by size:
3577 2623 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4720 4720
Size: 7777
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7777
PsiI TTA'TAA
Cuts at: 2063 5347 2063
Size: 3284 4493
PspOMI G'GGCC_C
Cuts at: 2625 2625
Size: 7777
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2082 2114 2082
Size: 32 7745
PstI C_TGCA'G
Cuts at: 830 2481 2544 3237 3889 5129 830
Size: 1651 63 693 652 1240 3478
Fragments arranged by size:
3478 1651 1240 693 652 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7777
PvuII CAG'CTG
Cuts at: 2458 2458
Size: 7777
SalI G'TCGA_C
Cuts at: 1936 5119 1936
Size: 3183 4594
SapI GCTCTTCn'nnn_
Cuts at: 2490 4611 2490
Size: 2121 5656
SbfI CC_TGCA'GG
Cuts at: 2481 5129 2481
Size: 2648 5129
ScaI AGT'ACT
Cuts at: 4924 6025 7737 4924
Size: 1101 1712 4964
Fragments arranged by size:
4964 1712 1101
SfcI C'TryA_G
Cuts at: 826 1045 2477 2540 3233 3885 4241 5125 6260
Size: 219 1432 63 693 652 356 884 1135
Cuts at: 6260 6941 7132 826
Size: 681 191 1471
Fragments arranged by size:
1471 1432 1135 884 693 681 652 356
219 191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7777
SmaI CCC'GGG
Cuts at: 1872 5104 1872
Size: 3232 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2222 2995 4225 4895 5881 6782
Size: 197 926 279 773 1230 670 986 901
Cuts at: 6782 7044 820
Size: 262 1553
Fragments arranged by size:
1553 1230 986 926 901 773 670 279
262 197
SnaBI TAC'GTA
Cuts at: 493 5568 493
Size: 5075 2702
SpeI A'CTAG_T
Cuts at: 152 5065 152
Size: 4913 2864
SphI G_CATG'C
Cuts at: 1132 5135 1132
Size: 4003 3774
SspI AAT'ATT
Cuts at: 5 52 4053 5701 5
Size: 47 4001 1648 2081
Fragments arranged by size:
4001 2081 1648 47
StuI AGG'CCT
Cuts at: 1517 3583 4362 1517
Size: 2066 779 4932
Fragments arranged by size:
4932 2066 779
StyI C'CwwG_G
Cuts at: 513 1065 2362 3408 5216 513
Size: 552 1297 1046 1808 3074
Fragments arranged by size:
3074 1808 1297 1046 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3041 4868 5004 5804 5821 5974 6159
Size: 1255 1461 1827 136 800 17 153 185
Cuts at: 6159 6604 325
Size: 445 1498
Fragments arranged by size:
1827 1498 1461 1255 800 445 185 153
136 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3348 3698 4922
Size: 274 80 33 51 1443 1371 350 1224
Cuts at: 4922 6023 7735 96
Size: 1101 1712 138
Fragments arranged by size:
1712 1443 1371 1224 1101 350 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4833 4865 7737 7769 4833
Size: 32 2872 32 4841
Fragments arranged by size:
4841 2872 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2265 1316
Size: 949 6828
XbaI T'CTAG_A
Cuts at: 5113 5113
Size: 7777
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7777
XmaI C'CCGG_G
Cuts at: 1870 5102 1870
Size: 3232 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4476 4605 4817 5906 4052
Size: 424 129 212 1089 5923
Fragments arranged by size:
5923 1089 424 212 129
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3384 5585 276
Size: 53 83 186 936 1850 2201 2468
Fragments arranged by size:
2468 2201 1850 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BsiWI BstXI
BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI