Restriction map for
pFN21AB0481
(Circular) (Six-base) MAPSORT of: pFN21AB0481.seq Check: 7340 from: 1 to: 6268
pFN21AB0481 6268 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2999 4078 278
Size: 53 83 186 936 1463 1079 2468
Fragments arranged by size:
2468 1463 1079 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3611 1937
Size: 1674 4594
Acc65I G'GTAC_C
Cuts at: 3589 3589
Size: 6268
AclI AA'CG_TT
Cuts at: 1563 4395 4768 1563
Size: 2832 373 3063
Fragments arranged by size:
3063 2832 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 3062 3436 4331 5715 5983 1632
Size: 705 725 374 895 1384 268 1917
Fragments arranged by size:
1917 1384 895 725 705 374 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6071
AflIII A'CryG_T
Cuts at: 1203 5167 6144 1203
Size: 3964 977 1327
Fragments arranged by size:
3964 1327 977
AgeI A'CCGG_T
Cuts at: 5099 5099
Size: 6268
AhdI GACnn_n'nnGTC
Cuts at: 2995 4997 2995
Size: 2002 4266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6268
AlwNI CAG_nnn'CTG
Cuts at: 3391 4011 5583 3391
Size: 620 1572 4076
Fragments arranged by size:
4076 1572 620
ApaI G_GGCC'C
Cuts at: 2351 2351
Size: 6268
ApaLI G'TGCA_C
Cuts at: 4325 5481 4325
Size: 1156 5112
ApoI r'AATT_y
Cuts at: 1493 2245 3304 3577 3803 3955 6064 1493
Size: 752 1059 273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 752 273 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4822 160
Size: 872 3790 1606
Fragments arranged by size:
3790 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2587 2687 3593 1870
Size: 73 586 58 100 906 4545
Fragments arranged by size:
4545 906 586 100 73 58
BamHI G'GATC_C
Cuts at: 3598 3965 3598
Size: 367 5901
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3084 3485 3589 5044
Size: 325 217 627 311 986 401 104 1455
Cuts at: 5044 618
Size: 1842
Fragments arranged by size:
1842 1455 986 627 401 325 311 217
104
BanII G_rGCy'C
Cuts at: 1391 2351 2576 3282 3587 1391
Size: 960 225 706 305 4072
Fragments arranged by size:
4072 960 706 305 225
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2012 928
Size: 971 113 5184
Fragments arranged by size:
5184 971 113
BbvCI CC'TCA_GC
Cuts at: 3473 3473
Size: 6268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4459 4493 1903
Size: 34 50 34 2438 34 3678
Fragments arranged by size:
3678 2438 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4160 5370 4160
Size: 1210 5058
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6268
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4879 136
Size: 107 122 71 631 605 337 2870 1525
Fragments arranged by size:
2870 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6263 6263
Size: 6268
BlpI GC'TnA_GC
Cuts at: 3326 3408 3685 3326
Size: 82 277 5909
Fragments arranged by size:
5909 277 82
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2103 2216 2351 3488 4329 5485 1790
Size: 281 32 113 135 1137 841 1156 2573
Fragments arranged by size:
2573 1156 1137 841 281 135 113 32
BmgBI CAC'GTC
Cuts at: 1318 2818 1318
Size: 1500 4768
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2869 3999 4957
Size: 416 53 83 399 24 1452 1130 958
Cuts at: 4957 442
Size: 1753
Fragments arranged by size:
1753 1452 1130 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2469 2690 3464 4928 1239
Size: 982 248 221 774 1464 2579
Fragments arranged by size:
2579 1464 982 774 248 221
Bpu10I CC'TnA_GC
Cuts at: 3473 3473
Size: 6268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4393 5258 5556 4393
Size: 865 298 5105
Fragments arranged by size:
5105 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2401 4931 882
Size: 617 902 2530 2219
Fragments arranged by size:
2530 2219 902 617
BsaAI yAC'GTr
Cuts at: 493 1204 4059 493
Size: 711 2855 2702
Fragments arranged by size:
2855 2702 711
BsaBI GATnn'nnATC
Cuts at: 3422 6262 3422
Size: 2840 3428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 2996 4075 4457
Size: 53 83 186 936 556 907 1079 382
Cuts at: 4457 5092 275
Size: 635 1451
Fragments arranged by size:
1451 1079 936 907 635 556 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2576 3354 4701 5099 5373 5520 5931 2576
Size: 778 1347 398 274 147 411 2913
Fragments arranged by size:
2913 1347 778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3158 3188 1178
Size: 30 165 30 1755 30 4258
Fragments arranged by size:
4258 1755 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3122 1186
Size: 1936 4332
BseYI C'CCAG_C
Cuts at: 2083 2694 3372 3561 5471 5967 2083
Size: 611 678 189 1910 496 2384
Fragments arranged by size:
2384 1910 678 611 496 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 3068 1163
Size: 573 798 534 4363
Fragments arranged by size:
4363 798 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3718 4479 5507 1540
Size: 275 186 1717 761 1028 2301
Fragments arranged by size:
2301 1717 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4329 4414 5485 4329
Size: 85 1071 5112
Fragments arranged by size:
5112 1071 85
BsmI GAATG_Cn'
Cuts at: 3779 3872 3779
Size: 93 6175
BsmBI CGTCTCn'nnnn_
Cuts at: 2096 2201 2096
Size: 105 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2103 2216 2351 2576 3282 3488
Size: 399 281 32 113 135 225 706 206
Cuts at: 3488 3587 4329 4414 5485 1391
Size: 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 399 281 225 206
135 113 99 85 32
BspEI T'CCGG_A
Cuts at: 2576 3354 5931 2576
Size: 778 2577 2913
Fragments arranged by size:
2913 2577 778
BspHI T'CATG_A
Cuts at: 4157 5887 4157
Size: 1730 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3556 3623 844
Size: 2712 67 3489
Fragments arranged by size:
3489 2712 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4155 1394
Size: 429 2332 3507
Fragments arranged by size:
3507 2332 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4763 4937 65
Size: 1183 3515 174 1396
Fragments arranged by size:
3515 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4912 5099 1700
Size: 110 3102 187 2869
Fragments arranged by size:
3102 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6268
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6268
BssSI C'ACGA_G
Cuts at: 4328 5340 4328
Size: 1012 5256
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3055 794
Size: 2261 4007
BstBI TT'CG_AA
Cuts at: 5072 5072
Size: 6268
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6268
BstXI CCAn_nnnn'nTGG
Cuts at: 2258 3244 3321 2258
Size: 986 77 5205
Fragments arranged by size:
5205 986 77
BstYI r'GATC_y
Cuts at: 1972 2154 3598 3965 4350 4367 5819 5905 5917
Size: 182 1444 367 385 17 1452 86 12
Cuts at: 5917 6263 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1444 385 367 346 182 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2899 513
Size: 552 1834 3882
Fragments arranged by size:
3882 1834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3492 487
Size: 683 56 2266 3263
Fragments arranged by size:
3263 2266 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 3017 3392 3784 4578 4598 2529
Size: 488 375 392 794 20 4199
Fragments arranged by size:
4199 794 488 392 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6233
DraI TTT'AAA
Cuts at: 3572 3572
Size: 6268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5275 809
Size: 507 3959 1802
Fragments arranged by size:
3959 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3105 3255
Size: 54 1425 50 275 18 180 1095 150
Cuts at: 3255 3715 4604 6233 8
Size: 460 889 1629 43
Fragments arranged by size:
1629 1425 1095 889 460 275 180 150
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3715 1537
Size: 275 1903 4090
Fragments arranged by size:
4090 1903 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4198 1353
Size: 2845 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3415 4855 5229 5375 387
Size: 1064 138 1826 1440 374 146 1280
Fragments arranged by size:
1826 1440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2337 2469 2690 3062 3436 3464
Size: 393 589 116 132 221 372 374 28
Cuts at: 3464 4331 4928 5715 5983 1239
Size: 867 597 787 268 1524
Fragments arranged by size:
1524 867 787 597 589 393 374 372
268 221 132 116 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2468
Size: 6268
EcoO109I rG'GnC_Cy
Cuts at: 2347 2348 2347
Size: 1 6267
EcoRI G'AATT_C
Cuts at: 3577 3955 3577
Size: 378 5890
EcoRV GAT'ATC
Cuts at: 3194 3194
Size: 6268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2246 2956 2246
Size: 710 5558
FspI TGC'GCA
Cuts at: 4774 4774
Size: 6268
HaeII r_GCGC'y
Cuts at: 1678 2659 5415 1678
Size: 981 2756 2531
Fragments arranged by size:
2756 2531 981
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2146 2178 2355
Size: 32 135 32 534 32 8 32 177
Cuts at: 2355 2387 2510 2542 2981 3013 3387 3419 3447
Size: 32 123 32 439 32 374 32 28
Cuts at: 3447 3479 4909 4941 4983 5015 1373
Size: 32 1430 32 42 32 2626
Fragments arranged by size:
2626 1430 534 439 374 177 135 123
42 32 32 32 32 32 32 32
32 32 32 32 28 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3612 3858 669
Size: 988 281 1674 246 3079
Fragments arranged by size:
3079 1674 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6268
HpaI GTT'AAC
Cuts at: 3858 3858
Size: 6268
KpnI G_GTAC'C
Cuts at: 3593 3593
Size: 6268
MfeI C'AATT_G
Cuts at: 3867 3867
Size: 6268
MluI A'CGCG_T
Cuts at: 6144 6144
Size: 6268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 3490 5382 5566 1305
Size: 1191 994 1892 184 2007
Fragments arranged by size:
2007 1892 1191 994 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 3107 3257 10
Size: 54 1425 343 180 1095 150 3021
Fragments arranged by size:
3021 1425 1095 343 180 150 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2969 3021 3176 3437 3506 4226 4585
Size: 648 1803 52 155 261 69 720 359
Cuts at: 4585 4744 518
Size: 159 2042
Fragments arranged by size:
2042 1803 720 648 359 261 159 155
69 52
MspA1I CmG'CkG
Cuts at: 1606 3121 3684 4361 5509 5754 1606
Size: 1515 563 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 677 563 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5716
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6268
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6268
NotI GC'GGCC_GC
Cuts at: 3715 3715
Size: 6268
NspI r_CATG'y
Cuts at: 1132 1229 3626 5171 1132
Size: 97 2397 1545 2229
Fragments arranged by size:
2397 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2073 2379 3094 1348
Size: 725 306 715 4522
Fragments arranged by size:
4522 725 715 306
PciI A'CATG_T
Cuts at: 5167 5167
Size: 6268
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2790 1108
Size: 1682 4586
PmeI GTTT'AAAC
Cuts at: 3572 3572
Size: 6268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2352 2384 4337 4369 5850 5882 2352
Size: 32 1953 32 1481 32 2738
Fragments arranged by size:
2738 1953 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6268
PsiI TTA'TAA
Cuts at: 3838 3838
Size: 6268
PspOMI G'GGCC_C
Cuts at: 2347 2347
Size: 6268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 6236
PstI C_TGCA'G
Cuts at: 830 3012 3189 3620 830
Size: 2182 177 431 3478
Fragments arranged by size:
3478 2182 431 177
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6268
PvuII CAG'CTG
Cuts at: 3121 3121
Size: 6268
SalI G'TCGA_C
Cuts at: 1936 3610 1936
Size: 1674 4594
SbfI CC_TGCA'GG
Cuts at: 3620 3620
Size: 6268
ScaI AGT'ACT
Cuts at: 4516 6228 4516
Size: 1712 4556
SfcI C'TryA_G
Cuts at: 826 1045 2911 3008 3116 3185 3616 4751 5432
Size: 219 1866 97 108 69 431 1135 681
Cuts at: 5432 5623 826
Size: 191 1471
Fragments arranged by size:
1866 1471 1135 681 431 219 191 108
97 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6268
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6268
SmaI CCC'GGG
Cuts at: 1872 3595 1872
Size: 1723 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4372 5273 5535 820
Size: 197 926 2429 901 262 1553
Fragments arranged by size:
2429 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4059 493
Size: 3566 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6268
SphI G_CATG'C
Cuts at: 1132 3626 1132
Size: 2494 3774
SspI AAT'ATT
Cuts at: 5 52 4192 5
Size: 47 4140 2081
Fragments arranged by size:
4140 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6268
StyI C'CwwG_G
Cuts at: 513 1065 2013 3707 513
Size: 552 948 1694 3074
Fragments arranged by size:
3074 1694 948 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 4295 4312 4465 4650 5095 325
Size: 1255 1281 1434 17 153 185 445 1498
Fragments arranged by size:
1498 1434 1281 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4514 6226 96
Size: 274 80 33 51 1443 2537 1712 138
Fragments arranged by size:
2537 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 2119 2151 2208 2240 3158 3190 6228
Size: 32 68 32 57 32 918 32 3038
Cuts at: 6228 6260 2019
Size: 32 2027
Fragments arranged by size:
3038 2027 918 68 57 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2537 1316
Size: 1221 5047
XbaI T'CTAG_A
Cuts at: 3604 3604
Size: 6268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2080 2897 3265 2080
Size: 817 368 5083
Fragments arranged by size:
5083 817 368
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6268
XmaI C'CCGG_G
Cuts at: 1870 3593 1870
Size: 1723 4545
XmnI GAAnn'nnTTC
Cuts at: 4397 4397
Size: 6268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2997 4076 276
Size: 53 83 186 936 1463 1079 2468
Fragments arranged by size:
2468 1463 1079 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI