Restriction map for
pFN21AB0479
(Circular) (Six-base) MAPSORT of: pFN21AB0479.seq Check: 3364 from: 1 to: 6259
pFN21AB0479 6259 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2217 2217
Size: 6259
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2671 2951 4322 5706 5974 1632
Size: 914 125 280 1371 1384 268 1917
Fragments arranged by size:
1917 1384 1371 914 280 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 5158 6135 1203
Size: 3955 977 1327
Fragments arranged by size:
3955 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 2702 1166
Size: 1536 4723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 2759 3242 1184
Size: 1206 369 483 4201
Fragments arranged by size:
4201 1206 483 369
AlwNI CAG_nnn'CTG
Cuts at: 2209 2393 2479 2929 3415 3538 4002 5574 2209
Size: 184 86 450 486 123 464 1572 2894
Fragments arranged by size:
2894 1572 486 464 450 184 123 86
ApaI G_GGCC'C
Cuts at: 2647 2886 2647
Size: 239 6020
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 3568 3794 3946 6055 1493
Size: 2075 226 152 2109 1697
Fragments arranged by size:
2109 2075 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2655 3506 3522 3584 1870
Size: 73 376 336 851 16 62 4545
Fragments arranged by size:
4545 851 376 336 73 62 16
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 2773 2871 3518 3580
Size: 325 217 627 926 60 98 647 62
Cuts at: 3580 5035 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 926 647 627 325 217 98
62 60
BanII G_rGCy'C
Cuts at: 1391 2647 2886 3240 3578 1391
Size: 1256 239 354 338 4072
Fragments arranged by size:
4072 1256 354 338 239
BbeI G_GCGC'C
Cuts at: 2875 3522 2875
Size: 647 5612
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2088 2122 4450 4484 1903
Size: 34 151 34 2328 34 3678
Fragments arranged by size:
3678 2328 151 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2353 4151 5361 2353
Size: 1798 1210 3251
Fragments arranged by size:
3251 1798 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2632 4870 136
Size: 107 122 71 631 605 960 2238 1525
Fragments arranged by size:
2238 1525 960 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2647 2845 2886 3023 4320 5476 1790
Size: 222 635 198 41 137 1297 1156 2573
Fragments arranged by size:
2573 1297 1156 635 222 198 137 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 2057 3990
Size: 416 53 83 399 24 615 25 1933
Cuts at: 3990 4948 442
Size: 958 1753
Fragments arranged by size:
1933 1753 958 615 416 399 83 53
25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2339 2729 3398 3560 4919 1239
Size: 1100 390 669 162 1359 2579
Fragments arranged by size:
2579 1359 1100 669 390 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 2250 4384 5249 5547 2182
Size: 68 2134 865 298 2894
Fragments arranged by size:
2894 2134 865 298 68
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2200 3125 4922 882
Size: 617 701 925 1797 2219
Fragments arranged by size:
2219 1797 925 701 617
BsaAI yAC'GTr
Cuts at: 493 1204 2940 4050 493
Size: 711 1736 1110 2702
Fragments arranged by size:
2702 1736 1110 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 2872 3519 4066
Size: 53 83 186 936 918 421 647 547
Cuts at: 4066 4448 5083 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 918 647 635 547 421 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3042 3336 4692 5090 5364 5511 5922 3042
Size: 294 1356 398 274 147 411 3379
Fragments arranged by size:
3379 1356 411 398 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 3122 3152 3440
Size: 30 165 30 1002 30 687 30 288
Cuts at: 3440 3470 1178
Size: 30 3967
Fragments arranged by size:
3967 1002 687 288 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3185 3404 1186
Size: 1999 219 4041
Fragments arranged by size:
4041 1999 219
BseYI C'CCAG_C
Cuts at: 2122 2166 2567 3204 5462 5958 2122
Size: 44 401 637 2258 496 2423
Fragments arranged by size:
2423 2258 637 496 401 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3401 1163
Size: 573 1665 4021
Fragments arranged by size:
4021 1665 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2639 3709 4470 5498 1540
Size: 275 186 638 1070 761 1028 2301
Fragments arranged by size:
2301 1070 1028 761 638 275 186
BsiHKAI G_wGCw'C
Cuts at: 3240 4320 4405 5476 3240
Size: 1080 85 1071 4023
Fragments arranged by size:
4023 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 3770 3863 3770
Size: 93 6166
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2647 2845 2886 3023 3240 3578
Size: 399 222 635 198 41 137 217 338
Cuts at: 3578 4320 4405 5476 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 399 338 222 217
198 137 85 41
BspEI T'CCGG_A
Cuts at: 3042 5922 3042
Size: 2880 3379
BspHI T'CATG_A
Cuts at: 3426 4148 5878 3426
Size: 722 1730 3807
Fragments arranged by size:
3807 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 3614 844
Size: 1373 1397 3489
Fragments arranged by size:
3489 1397 1373
BsrBI CCG'CTC
Cuts at: 1394 1823 2416 3030 3442 4146 1394
Size: 429 593 614 412 704 3507
Fragments arranged by size:
3507 704 614 593 429 412
BsrDI GCAATG_nn'
Cuts at: 65 1248 3292 4754 4928 65
Size: 1183 2044 1462 174 1396
Fragments arranged by size:
2044 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3281 4903 5090 1700
Size: 110 1471 1622 187 2869
Fragments arranged by size:
2869 1622 1471 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 3415 794
Size: 1415 1206 3638
Fragments arranged by size:
3638 1415 1206
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 3142 3142
Size: 6259
BstYI r'GATC_y
Cuts at: 1972 3333 3589 3956 4341 4358 5810 5896 5908
Size: 1361 256 367 385 17 1452 86 12
Cuts at: 5908 6254 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1361 385 367 346 256 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2277 2335 2509 2863 513
Size: 552 1212 58 174 354 3909
Fragments arranged by size:
3909 1212 552 354 174 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2442 487
Size: 683 56 1216 4304
Fragments arranged by size:
4304 1216 683 56
BtsI GCAGTG_nn'
Cuts at: 2104 2219 2843 3775 4569 4589 2104
Size: 115 624 932 794 20 3774
Fragments arranged by size:
3774 932 794 624 115 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DraIII CAC_nnn'GTG
Cuts at: 2390 2390
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2636 2751 3369
Size: 54 1425 50 275 18 806 115 618
Cuts at: 3369 3706 4595 6224 8
Size: 337 889 1629 43
Fragments arranged by size:
1629 1425 889 806 618 337 275 115
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2636 3706 1537
Size: 275 824 1070 4090
Fragments arranged by size:
4090 1070 824 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2900 4189 1353
Size: 1547 1289 3423
Fragments arranged by size:
3423 1547 1289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2273 3448 4846 5220 5366 387
Size: 1064 138 684 1175 1398 374 146 1280
Fragments arranged by size:
1398 1280 1175 1064 684 374 146 138
EcoICRI GAG'CTC
Cuts at: 3238 3238
Size: 6259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2546 2671 2729 2951 3398 3560
Size: 393 707 207 125 58 222 447 162
Cuts at: 3560 4322 4919 5706 5974 1239
Size: 762 597 787 268 1524
Fragments arranged by size:
1524 787 762 707 597 447 393 268
222 207 162 125 58
EcoO109I rG'GnC_Cy
Cuts at: 2059 2127 2440 2514 2625 2643 2883 3503 3535
Size: 68 313 74 111 18 240 620 32
Cuts at: 3535 2059
Size: 4783
Fragments arranged by size:
4783 620 313 240 111 74 68 32
18
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 6259
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 2875 3191 3522 5406 1678
Size: 1197 316 331 1884 2531
Fragments arranged by size:
2531 1884 1197 331 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 2441 2473 3161
Size: 32 135 32 833 32 4 32 688
Cuts at: 3161 3193 3440 3472 4900 4932 4974 5006 1373
Size: 32 247 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 833 688 247 135 42 32
32 32 32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6259
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KasI G'GCGC_C
Cuts at: 2871 3518 2871
Size: 647 5612
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 2427 5373 5557 1305
Size: 748 374 2946 184 2007
Fragments arranged by size:
2946 2007 748 374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4437
Fragments arranged by size:
4437 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 4217 4576 4735 518
Size: 648 1536 1515 359 159 2042
Fragments arranged by size:
2042 1536 1515 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2337 2749 2770 2986 3250 3368 3675 4352
Size: 731 412 21 216 264 118 307 677
Cuts at: 4352 5500 5745 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 731 677 412 307 264 245
216 118 21
NarI GG'CG_CC
Cuts at: 2872 3519 2872
Size: 647 5612
NcoI C'CATG_G
Cuts at: 513 1065 2277 2509 2863 513
Size: 552 1212 232 354 3909
Fragments arranged by size:
3909 1212 552 354 232
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 2502 3617 5162 1132
Size: 97 1273 1115 1545 2229
Fragments arranged by size:
2229 1545 1273 1115 97
PasI CC'CwG_GG
Cuts at: 1348 2473 2629 1348
Size: 1125 156 4978
Fragments arranged by size:
4978 1125 156
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2278 2479 2619 3538 1108
Size: 1170 201 140 919 3829
Fragments arranged by size:
3829 1170 919 201 140
PfoI T'CCnGG_A
Cuts at: 2355 3117 3375 2355
Size: 762 258 5239
Fragments arranged by size:
5239 762 258
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2440 2059
Size: 381 5878
PshAI GACnn'nnGTC
Cuts at: 1613 2279 1613
Size: 666 5593
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PspOMI G'GGCC_C
Cuts at: 2643 2882 2643
Size: 239 6020
PstI C_TGCA'G
Cuts at: 830 2748 2838 3411 3611 830
Size: 1918 90 573 200 3478
Fragments arranged by size:
3478 1918 573 200 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 2749 2986 3250 2749
Size: 237 264 5758
Fragments arranged by size:
5758 264 237
SacI G_AGCT'C
Cuts at: 3240 3240
Size: 6259
SacII CC_GC'GG
Cuts at: 2338 2338
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SanDI GG'GwC_CC
Cuts at: 2059 2059
Size: 6259
SapI GCTCTTCn'nnn_
Cuts at: 2900 2900
Size: 6259
SbfI CC_TGCA'GG
Cuts at: 3411 3611 3411
Size: 200 6059
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SexAI A'CCwGG_T
Cuts at: 2386 2386
Size: 6259
SfcI C'TryA_G
Cuts at: 826 1045 2584 2744 2834 3407 3607 4742 5423
Size: 219 1539 160 90 573 200 1135 681
Cuts at: 5423 5614 826
Size: 191 1471
Fragments arranged by size:
1539 1471 1135 681 573 219 200 191
160 90
SfoI GGC'GCC
Cuts at: 2873 3520 2873
Size: 647 5612
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 2657 3524 3586 1872
Size: 785 867 62 4545
Fragments arranged by size:
4545 867 785 62
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 2265 2319 4363 5264 5526
Size: 197 926 254 68 54 2044 901 262
Cuts at: 5526 820
Size: 1553
Fragments arranged by size:
2044 1553 926 901 262 254 197 68
54
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2277 2509 2613 2863 3698 513
Size: 552 1212 232 104 250 835 3074
Fragments arranged by size:
3074 1212 835 552 250 232 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2275 4286 4303 4456 4641 5086 325
Size: 1255 695 2011 17 153 185 445 1498
Fragments arranged by size:
2011 1498 1255 695 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4505 6217 96
Size: 274 80 33 51 1443 2528 1712 138
Fragments arranged by size:
2528 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6259
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3008 3008
Size: 6259
XhoI C'TCGA_G
Cuts at: 1943 2319 1943
Size: 376 5883
XmaI C'CCGG_G
Cuts at: 1870 2655 3522 3584 1870
Size: 785 867 62 4545
Fragments arranged by size:
4545 867 785 62
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SfiI SrfI SwaI