Restriction map for
pFN21AB0470
(Circular) (Six-base) MAPSORT of: pFN21AB0470.seq Check: 2945 from: 1 to: 7678
pFN21AB0470 7678 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 3601 2284
Size: 1317 6361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5488 278
Size: 53 83 186 936 3952 2468
Fragments arranged by size:
3952 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4233 5021 1937
Size: 2296 788 4594
Fragments arranged by size:
4594 2296 788
Acc65I G'GTAC_C
Cuts at: 4999 4999
Size: 7678
AclI AA'CG_TT
Cuts at: 1563 5805 6178 1563
Size: 4242 373 3063
Fragments arranged by size:
4242 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2618 3360 3728 4947 5741 7125 7393 1632
Size: 986 742 368 1219 794 1384 268 1917
Fragments arranged by size:
1917 1384 1219 986 794 742 368 268
AfeI AGC'GCT
Cuts at: 2243 2243
Size: 7678
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7481
AflIII A'CryG_T
Cuts at: 1203 6577 7554 1203
Size: 5374 977 1327
Fragments arranged by size:
5374 1327 977
AgeI A'CCGG_T
Cuts at: 6509 6509
Size: 7678
AhdI GACnn_n'nnGTC
Cuts at: 6407 6407
Size: 7678
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2537 2761 1184
Size: 1353 224 6101
Fragments arranged by size:
6101 1353 224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2422 2454 2422
Size: 32 7646
AlwNI CAG_nnn'CTG
Cuts at: 2088 2203 2585 2818 3487 3617 4600 4822 4865
Size: 115 382 233 669 130 983 222 43
Cuts at: 4865 5421 6993 2088
Size: 556 1572 2773
Fragments arranged by size:
2773 1572 983 669 556 382 233 222
130 115 43
ApaI G_GGCC'C
Cuts at: 2948 3108 3239 2948
Size: 160 131 7387
Fragments arranged by size:
7387 160 131
ApaLI G'TGCA_C
Cuts at: 4244 4790 5735 6891 4244
Size: 546 945 1156 5031
Fragments arranged by size:
5031 1156 945 546
ApoI r'AATT_y
Cuts at: 1493 4987 5213 5365 7474 1493
Size: 3494 226 152 2109 1697
Fragments arranged by size:
3494 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6232 160
Size: 872 5200 1606
Fragments arranged by size:
5200 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7678
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2285 2949 3008 3095 3504 3567
Size: 73 98 244 664 59 87 409 63
Cuts at: 3567 3630 4336 4479 5003 1870
Size: 63 706 143 524 4545
Fragments arranged by size:
4545 706 664 524 409 244 143 98
87 73 63 63 59
BamHI G'GATC_C
Cuts at: 4432 4714 5008 5375 4432
Size: 282 294 367 6735
Fragments arranged by size:
6735 367 294 282
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 3020 3552 3741 3890
Size: 325 217 627 258 975 532 189 149
Cuts at: 3890 4259 4846 4999 6454 618
Size: 369 587 153 1455 1842
Fragments arranged by size:
1842 1455 975 627 587 532 369 325
258 217 189 153 149
BanII G_rGCy'C
Cuts at: 1391 2042 2160 2172 2256 2478 2592 2795 2948
Size: 651 118 12 84 222 114 203 153
Cuts at: 2948 2972 3009 3108 3201 3239 3574 3801 3942
Size: 24 37 99 93 38 335 227 141
Cuts at: 3942 4997 1391
Size: 1055 4072
Fragments arranged by size:
4072 1055 651 335 227 222 203 153
141 118 114 99 93 84 38 37
24 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3348 3570 928
Size: 971 1449 222 5036
Fragments arranged by size:
5036 1449 971 222
BbvCI CC'TCA_GC
Cuts at: 2109 4530 2109
Size: 2421 5257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5869 5903 1903
Size: 34 50 34 3848 34 3678
Fragments arranged by size:
3848 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5570 6780 4577
Size: 993 1210 5475
Fragments arranged by size:
5475 1210 993
BclI T'GATC_A
Cuts at: 1548 3282 1548
Size: 1734 5944
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2260 3626
Size: 107 122 71 631 605 395 193 1366
Cuts at: 3626 4040 4679 4688 6289 136
Size: 414 639 9 1601 1525
Fragments arranged by size:
1601 1525 1366 639 631 605 414 395
193 122 107 71 9
BglII A'GATC_T
Cuts at: 7673 7673
Size: 7678
BlpI GC'TnA_GC
Cuts at: 2510 3902 5095 2510
Size: 1392 1193 5093
Fragments arranged by size:
5093 1392 1193
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2948 3023 3096 3108 3239 3555 4248
Size: 260 898 75 73 12 131 316 693
Cuts at: 4248 4794 5739 6895 1790
Size: 546 945 1156 2573
Fragments arranged by size:
2573 1156 945 898 693 546 316 260
131 75 73 12
BmgBI CAC'GTC
Cuts at: 1318 3274 4243 1318
Size: 1956 969 4753
Fragments arranged by size:
4753 1956 969
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2144 2538 3121
Size: 416 53 83 399 24 727 394 583
Cuts at: 3121 3204 4246 4281 4559 4813 5409 6367 442
Size: 83 1042 35 278 254 596 958 1753
Fragments arranged by size:
1753 1042 958 727 596 583 416 399
394 278 254 83 83 53 35 24
BmtI G_CTAG'C
Cuts at: 1056 2390 1056
Size: 1334 6344
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3169 3204 3169
Size: 35 7643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2501 2648 3275 3368 3413 3431 3625
Size: 1253 9 147 627 93 45 18 194
Cuts at: 3625 4428 4476 4793 6338 1239
Size: 803 48 317 1545 2579
Fragments arranged by size:
2579 1545 1253 803 627 317 194 147
93 48 45 18 9
Bpu10I CC'TnA_GC
Cuts at: 2109 4530 2109
Size: 2421 5257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5803 6668 6966 5803
Size: 865 298 6515
Fragments arranged by size:
6515 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2690 3644 6341 882
Size: 617 1191 954 2697 2219
Fragments arranged by size:
2697 2219 1191 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3877 5469 493
Size: 711 2673 1592 2702
Fragments arranged by size:
2702 2673 1592 711
BsaBI GATnn'nnATC
Cuts at: 4472 7672 4472
Size: 3200 4478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4671 4901 5485 5867
Size: 53 83 186 936 3138 230 584 382
Cuts at: 5867 6502 275
Size: 635 1451
Fragments arranged by size:
3138 1451 936 635 584 382 230 186
83 53
BsaWI w'CCGG_w
Cuts at: 3801 6111 6509 6783 6930 7341 3801
Size: 2310 398 274 147 411 4138
Fragments arranged by size:
4138 2310 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3704 3734 3796 3826 4608
Size: 30 165 30 2301 30 62 30 782
Cuts at: 4608 4638 1178
Size: 30 4218
Fragments arranged by size:
4218 2301 782 165 62 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 3041 3432 3872 4970 1186
Size: 1515 340 391 440 1098 3894
Fragments arranged by size:
3894 1515 1098 440 391 340
BseYI C'CCAG_C
Cuts at: 2196 2534 2672 3014 3406 3491 3583 4038 4263
Size: 338 138 342 392 85 92 455 225
Cuts at: 4263 6881 7377 2196
Size: 2618 496 2497
Fragments arranged by size:
2618 2497 496 455 392 342 338 225
138 92 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2189 1163
Size: 573 376 77 6652
Fragments arranged by size:
6652 573 376 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3760 5128 5889 6917 1540
Size: 275 186 1759 1368 761 1028 2301
Fragments arranged by size:
2301 1759 1368 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2562 2772 3399 3722 3753 3882 4248 4296 4794
Size: 210 627 323 31 129 366 48 498
Cuts at: 4794 5739 5824 6895 2562
Size: 945 85 1071 3345
Fragments arranged by size:
3345 1071 945 627 498 366 323 210
129 85 48 31
BsmI GAATG_Cn'
Cuts at: 2454 5189 5282 2454
Size: 2735 93 4850
Fragments arranged by size:
4850 2735 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2477
Size: 7678
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2050 2160 2172 2256 2478 2562
Size: 399 252 8 110 12 84 222 84
Cuts at: 2562 2592 2772 2795 2948 2972 3009 3023 3096
Size: 30 180 23 153 24 37 14 73
Cuts at: 3096 3108 3201 3239 3399 3555 3574 3722 3753
Size: 12 93 38 160 156 19 148 31
Cuts at: 3753 3801 3882 3942 4248 4296 4794 4997 5739
Size: 48 81 60 306 48 498 203 742
Cuts at: 5739 5824 6895 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 306 252 222
203 180 160 156 153 148 110 93
85 84 84 81 73 60 48 48
38 37 31 30 24 23 19 14
12 12 8
BspEI T'CCGG_A
Cuts at: 3801 7341 3801
Size: 3540 4138
BspHI T'CATG_A
Cuts at: 5567 7297 5567
Size: 1730 5948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2284 3601 5033 844
Size: 1292 148 1317 1432 3489
Fragments arranged by size:
3489 1432 1317 1292 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2502 2632 3148 3221 3440 3793 3871
Size: 429 679 130 516 73 219 353 78
Cuts at: 3871 3901 4767 5565 1394
Size: 30 866 798 3507
Fragments arranged by size:
3507 866 798 679 516 429 353 219
130 78 73 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2174 6173 6347 65
Size: 1183 926 3999 174 1396
Fragments arranged by size:
3999 1396 1183 926 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3555 3755 4188 6322 6509 1700
Size: 110 1745 200 433 2134 187 2869
Fragments arranged by size:
2869 2134 1745 433 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7678
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7678
BssSI C'ACGA_G
Cuts at: 4083 5738 6750 4083
Size: 1655 1012 5011
Fragments arranged by size:
5011 1655 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7678
BstBI TT'CG_AA
Cuts at: 6482 6482
Size: 7678
BstEII G'GTnAC_C
Cuts at: 1184 4535 1184
Size: 3351 4327
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2371 4842 2179
Size: 192 2471 5015
Fragments arranged by size:
5015 2471 192
BstYI r'GATC_y
Cuts at: 1972 4221 4432 4714 5008 5375 5760 5777 7229
Size: 2249 211 282 294 367 385 17 1452
Cuts at: 7229 7315 7327 7673 1972
Size: 86 12 346 1977
Fragments arranged by size:
2249 1977 1452 385 367 346 294 282
211 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3064 3423 3459 3064
Size: 359 36 7283
Fragments arranged by size:
7283 359 36
BtgI C'CryG_G
Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425
Size: 552 1056 147 727 417 737 100 176
Cuts at: 4425 513
Size: 3766
Fragments arranged by size:
3766 1056 737 727 552 417 176 147
100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4165 4295 4459 487
Size: 683 56 2939 130 164 3706
Fragments arranged by size:
3706 2939 683 164 130 56
BtsI GCAGTG_nn'
Cuts at: 2645 4057 4629 4893 5194 5988 6008 2645
Size: 1412 572 264 301 794 20 4315
Fragments arranged by size:
4315 1412 794 572 301 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7643
DraI TTT'AAA
Cuts at: 4982 4982
Size: 7678
DraIII CAC_nnn'GTG
Cuts at: 4553 4553
Size: 7678
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6685 809
Size: 507 5369 1802
Fragments arranged by size:
5369 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3173 3757 4176
Size: 54 1425 50 275 18 1343 584 419
Cuts at: 4176 4645 5125 6014 7643 8
Size: 469 480 889 1629 43
Fragments arranged by size:
1629 1425 1343 889 584 480 469 419
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3757 5125 1537
Size: 275 1945 1368 4090
Fragments arranged by size:
4090 1945 1368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2990 3840 4701 5608 1353
Size: 869 768 850 861 907 3423
Fragments arranged by size:
3423 907 869 861 850 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6265 6639 6785 387
Size: 1064 138 4676 374 146 1280
Fragments arranged by size:
4676 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2501 2618 2648 3275 3360 3368
Size: 393 860 9 117 30 627 85 8
Cuts at: 3368 3413 3431 3625 3728 4428 4476 4793 4947
Size: 45 18 194 103 700 48 317 154
Cuts at: 4947 5741 6338 7125 7393 1239
Size: 794 597 787 268 1524
Fragments arranged by size:
1524 860 794 787 700 627 597 393
317 268 194 154 117 103 85 48
45 30 18 9 8
EcoO109I rG'GnC_Cy
Cuts at: 2028 2085 2105 2300 2928 2945 3105 3236 3434
Size: 57 20 195 628 17 160 131 198
Cuts at: 3434 3663 4935 2028
Size: 229 1272 4771
Fragments arranged by size:
4771 1272 628 229 198 195 160 131
57 20 17
EcoRI G'AATT_C
Cuts at: 4987 5365 4987
Size: 378 7300
FspI TGC'GCA
Cuts at: 2573 6184 2573
Size: 3611 4067
HaeII r_GCGC'y
Cuts at: 1678 2022 2203 2245 2804 4822 6825 1678
Size: 344 181 42 559 2018 2003 2531
Fragments arranged by size:
2531 2018 2003 559 344 181 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2626 2658 3839 3871 4922
Size: 32 135 32 1054 32 1181 32 1051
Cuts at: 4922 4954 6319 6351 6393 6425 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 1181 1054 1051 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5022 5268 669
Size: 988 281 3084 246 3079
Fragments arranged by size:
3084 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7678
HpaI GTT'AAC
Cuts at: 5268 5268
Size: 7678
KpnI G_GTAC'C
Cuts at: 5003 5003
Size: 7678
MfeI C'AATT_G
Cuts at: 5277 5277
Size: 7678
MluI A'CGCG_T
Cuts at: 7554 7554
Size: 7678
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2960 6792 6976 1305
Size: 1655 3832 184 2007
Fragments arranged by size:
3832 2007 1655 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3175 4178 4647 10
Size: 54 1425 343 1343 1003 469 3041
Fragments arranged by size:
3041 1425 1343 1003 469 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 2743 4314 5636 5995 6154 518
Size: 648 1113 464 1571 1322 359 159 2042
Fragments arranged by size:
2042 1571 1322 1113 648 464 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2815 3082 3952 4038 4046 4195 4374
Size: 537 672 267 870 86 8 149 179
Cuts at: 4374 4948 5094 5771 6919 7164 1606
Size: 574 146 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 672 574 537 267
245 179 149 146 86 8
NaeI GCC'GGC
Cuts at: 3757 4190 3757
Size: 433 7245
NcoI C'CATG_G
Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425
Size: 552 1056 147 727 417 737 100 176
Cuts at: 4425 513
Size: 3766
Fragments arranged by size:
3766 1056 737 727 552 417 176 147
100
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7678
NgoMIV G'CCGG_C
Cuts at: 3755 4188 3755
Size: 433 7245
NheI G'CTAG_C
Cuts at: 1052 2386 1052
Size: 1334 6344
NotI GC'GGCC_GC
Cuts at: 5125 5125
Size: 7678
NspI r_CATG'y
Cuts at: 1132 1229 4816 5036 6581 1132
Size: 97 3587 220 1545 2229
Fragments arranged by size:
3587 2229 1545 220 97
PasI CC'CwG_GG
Cuts at: 1348 3544 1348
Size: 2196 5482
PciI A'CATG_T
Cuts at: 6577 6577
Size: 7678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3001 3379 4553 1108
Size: 1893 378 1174 4233
Fragments arranged by size:
4233 1893 1174 378
PfoI T'CCnGG_A
Cuts at: 3054 3054
Size: 7678
PmeI GTTT'AAAC
Cuts at: 4982 4982
Size: 7678
PmlI CAC'GTG
Cuts at: 3877 3877
Size: 7678
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5747 5779 7260 7292 5747
Size: 32 1481 32 6133
Fragments arranged by size:
6133 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2300 2928 3663 2028
Size: 272 628 735 6043
Fragments arranged by size:
6043 735 628 272
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7678
PsiI TTA'TAA
Cuts at: 5248 5248
Size: 7678
PspOMI G'GGCC_C
Cuts at: 2944 3104 3235 2944
Size: 160 131 7387
Fragments arranged by size:
7387 160 131
PstI C_TGCA'G
Cuts at: 830 2060 2190 2235 2829 3042 3789 3855 5030
Size: 1230 130 45 594 213 747 66 1175
Cuts at: 5030 830
Size: 3478
Fragments arranged by size:
3478 1230 1175 747 594 213 130 66
45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7678
PvuII CAG'CTG
Cuts at: 4195 4948 4195
Size: 753 6925
SalI G'TCGA_C
Cuts at: 1936 5020 1936
Size: 3084 4594
SanDI GG'GwC_CC
Cuts at: 2028 2300 2028
Size: 272 7406
SapI GCTCTTCn'nnn_
Cuts at: 3840 4701 3840
Size: 861 6817
SbfI CC_TGCA'GG
Cuts at: 5030 5030
Size: 7678
ScaI AGT'ACT
Cuts at: 5926 7638 5926
Size: 1712 5966
SfcI C'TryA_G
Cuts at: 826 1045 2056 2186 2231 2358 2461 2825 3038
Size: 219 1011 130 45 127 103 364 213
Cuts at: 3038 3785 3851 5026 6161 6842 7033 826
Size: 747 66 1175 1135 681 191 1471
Fragments arranged by size:
1471 1175 1135 1011 747 681 364 219
213 191 130 127 103 66 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7678
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7678
SmaI CCC'GGG
Cuts at: 1872 2043 3097 3506 3569 4338 5005 1872
Size: 171 1054 409 63 769 667 4545
Fragments arranged by size:
4545 1054 769 667 409 171 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5782 6683 6945 820
Size: 197 926 3839 901 262 1553
Fragments arranged by size:
3839 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5469 493
Size: 4976 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7678
SphI G_CATG'C
Cuts at: 1132 4816 5036 1132
Size: 3684 220 3774
Fragments arranged by size:
3774 3684 220
SrfI GCCC'GGGC
Cuts at: 3097 3097
Size: 7678
SspI AAT'ATT
Cuts at: 5 52 5602 5
Size: 47 5550 2081
Fragments arranged by size:
5550 2081 47
StuI AGG'CCT
Cuts at: 1517 2229 3165 3836 4682 1517
Size: 712 936 671 846 4513
Fragments arranged by size:
4513 936 846 712 671
StyI C'CwwG_G
Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425
Size: 552 1056 147 727 417 737 100 176
Cuts at: 4425 4635 5117 513
Size: 210 482 3074
Fragments arranged by size:
3074 1056 737 727 552 482 417 210
176 147 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3688 3847 4277 4541 4834 5705 5722
Size: 1255 2108 159 430 264 293 871 17
Cuts at: 5722 5875 6060 6505 325
Size: 153 185 445 1498
Fragments arranged by size:
2108 1498 1255 871 445 430 293 264
185 159 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5924 7636 96
Size: 274 80 33 51 1443 3947 1712 138
Fragments arranged by size:
3947 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3796 3828 4608 4640 7638 7670 3704
Size: 32 60 32 780 32 2998 32 3712
Fragments arranged by size:
3712 2998 780 60 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2863 3698 4499 1316
Size: 1547 835 801 4495
Fragments arranged by size:
4495 1547 835 801
XbaI T'CTAG_A
Cuts at: 5014 5014
Size: 7678
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2623 2785 3052 4768 2623
Size: 162 267 1716 5533
Fragments arranged by size:
5533 1716 267 162
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7678
XmaI C'CCGG_G
Cuts at: 1870 2041 3095 3504 3567 4336 5003 1870
Size: 171 1054 409 63 769 667 4545
Fragments arranged by size:
4545 1054 769 667 409 171 63
XmnI GAAnn'nnTTC
Cuts at: 4100 5807 4100
Size: 1707 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5486 276
Size: 53 83 186 936 3952 2468
Fragments arranged by size:
3952 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SexAI
SfiI SfoI SwaI