Restriction map for
pFN21AB0469
(Circular) (Six-base) MAPSORT of: pFN21AB0469.seq Check: 3393 from: 1 to: 6250
pFN21AB0469 6250 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4060 278
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3593 1937
Size: 1656 4594
Acc65I G'GTAC_C
Cuts at: 2425 3571 2425
Size: 1146 5104
AclI AA'CG_TT
Cuts at: 1563 4377 4750 1563
Size: 2814 373 3063
Fragments arranged by size:
3063 2814 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2244 3326 3356 4313 5697 5965 1632
Size: 612 1082 30 957 1384 268 1917
Fragments arranged by size:
1917 1384 1082 957 612 268 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6053
AflIII A'CryG_T
Cuts at: 1203 5149 6126 1203
Size: 3946 977 1327
Fragments arranged by size:
3946 1327 977
AgeI A'CCGG_T
Cuts at: 5081 5081
Size: 6250
AhdI GACnn_n'nnGTC
Cuts at: 4979 4979
Size: 6250
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6250
AlwNI CAG_nnn'CTG
Cuts at: 3993 5565 3993
Size: 1572 4678
ApaI G_GGCC'C
Cuts at: 2093 2129 2093
Size: 36 6214
ApaLI G'TGCA_C
Cuts at: 4307 5463 4307
Size: 1156 5094
ApoI r'AATT_y
Cuts at: 1493 2397 2553 3217 3559 3785 3937 6046 1493
Size: 904 156 664 342 226 152 2109 1697
Fragments arranged by size:
2109 1697 904 664 342 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 3123 4804 160
Size: 872 2091 1681 1606
Fragments arranged by size:
2091 1681 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2092 3575 1870
Size: 73 70 79 1483 4545
Fragments arranged by size:
4545 1483 79 73 70
BamHI G'GATC_C
Cuts at: 3580 3947 3580
Size: 367 5883
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 2425 3571 5026 618
Size: 325 217 627 261 377 1146 1455 1842
Fragments arranged by size:
1842 1455 1146 627 377 325 261 217
BanII G_rGCy'C
Cuts at: 1391 2093 2129 3466 3569 1391
Size: 702 36 1337 103 4072
Fragments arranged by size:
4072 1337 702 103 36
BbeI G_GCGC'C
Cuts at: 2052 2052
Size: 6250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3212 3314 928
Size: 971 1313 102 3864
Fragments arranged by size:
3864 1313 971 102
BbvCI CC'TCA_GC
Cuts at: 2319 3078 2319
Size: 759 5491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4441 4475 1903
Size: 34 2504 34 3678
Fragments arranged by size:
3678 2504 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4142 5352 4142
Size: 1210 5040
BclI T'GATC_A
Cuts at: 1548 2946 3151 1548
Size: 1398 205 4647
Fragments arranged by size:
4647 1398 205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4861 136
Size: 107 122 71 631 605 337 2852 1525
Fragments arranged by size:
2852 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3039 6245 3039
Size: 3206 3044
BlpI GC'TnA_GC
Cuts at: 3329 3667 3329
Size: 338 5912
Bme1580I G_kGCm'C
Cuts at: 1790 2093 2129 4311 5467 1790
Size: 303 36 2182 1156 2573
Fragments arranged by size:
2573 2182 1156 303 36
BmgBI CAC'GTC
Cuts at: 1318 2836 1318
Size: 1518 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 2878 3373
Size: 416 53 83 399 24 858 603 495
Cuts at: 3373 3981 4939 442
Size: 608 958 1753
Fragments arranged by size:
1753 958 858 608 603 495 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2672 2972 4910 1239
Size: 1433 300 1938 2579
Fragments arranged by size:
2579 1938 1433 300
Bpu10I CC'TnA_GC
Cuts at: 2319 3078 3465 2319
Size: 759 387 5104
Fragments arranged by size:
5104 759 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3377 4375 5240 5538 3377
Size: 998 865 298 4089
Fragments arranged by size:
4089 998 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2176 2302 2795 3336 4913 882
Size: 617 677 126 493 541 1577 2219
Fragments arranged by size:
2219 1577 677 617 541 493 126
BsaAI yAC'GTr
Cuts at: 493 1204 4041 493
Size: 711 2837 2702
Fragments arranged by size:
2837 2702 711
BsaBI GATnn'nnATC
Cuts at: 6244 6244
Size: 6250
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 4057 4439 5074
Size: 53 83 186 936 516 2008 382 635
Cuts at: 5074 275
Size: 1451
Fragments arranged by size:
2008 1451 936 635 516 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3540 4683 5081 5355 5502 5913 3540
Size: 1143 398 274 147 411 3877
Fragments arranged by size:
3877 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2970 3000 3461 3476 3491
Size: 30 165 30 1567 30 461 15 15
Cuts at: 3491 3506 1178
Size: 15 3922
Fragments arranged by size:
3922 1567 461 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3440 3443 1186
Size: 2254 3 3993
Fragments arranged by size:
3993 2254 3
BseYI C'CCAG_C
Cuts at: 5453 5949 5453
Size: 496 5754
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3403 3521 1163
Size: 573 1667 118 3892
Fragments arranged by size:
3892 1667 573 118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2828 3700 4461 5489 1540
Size: 275 186 827 872 761 1028 2301
Fragments arranged by size:
2301 1028 872 827 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4311 4396 5467 4311
Size: 85 1071 5094
Fragments arranged by size:
5094 1071 85
BsmI GAATG_Cn'
Cuts at: 3761 3854 3761
Size: 93 6157
BsmBI CGTCTCn'nnnn_
Cuts at: 2826 3502 2826
Size: 676 5574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2129 3466 3569 4311 4396 5467
Size: 399 303 36 1337 103 742 85 1071
Cuts at: 5467 1391
Size: 2174
Fragments arranged by size:
2174 1337 1071 742 399 303 103 85
36
BspEI T'CCGG_A
Cuts at: 3540 5913 3540
Size: 2373 3877
BspHI T'CATG_A
Cuts at: 2487 4139 5869 2487
Size: 1652 1730 2868
Fragments arranged by size:
2868 1730 1652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 2323 3039 3605 844
Size: 1476 3 716 566 3489
Fragments arranged by size:
3489 1476 716 566 3
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 2171 3004 4137 1394
Size: 429 331 17 833 1133 3507
Fragments arranged by size:
3507 1133 833 429 331 17
BsrDI GCAATG_nn'
Cuts at: 65 1248 4745 4919 65
Size: 1183 3497 174 1396
Fragments arranged by size:
3497 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4894 5081 1700
Size: 110 3084 187 2869
Fragments arranged by size:
3084 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6250
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6250
BssSI C'ACGA_G
Cuts at: 2999 4310 5322 2999
Size: 1311 1012 3927
Fragments arranged by size:
3927 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6250
BstBI TT'CG_AA
Cuts at: 5054 5054
Size: 6250
BstEII G'GTnAC_C
Cuts at: 1184 3416 1184
Size: 2232 4018
BstYI r'GATC_y
Cuts at: 1972 2325 2449 3039 3072 3273 3580 3947 4332
Size: 353 124 590 33 201 307 367 385
Cuts at: 4332 4349 5801 5887 5899 6245 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 590 385 367 353 346 307
201 124 86 33 17 12
Bsu36I CC'TnA_GG
Cuts at: 2059 2217 3348 2059
Size: 158 1131 4961
Fragments arranged by size:
4961 1131 158
BtgI C'CryG_G
Cuts at: 513 1065 2004 2229 513
Size: 552 939 225 4534
Fragments arranged by size:
4534 939 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5511
Fragments arranged by size:
5511 683 56
BtsI GCAGTG_nn'
Cuts at: 3234 3766 4560 4580 3234
Size: 532 794 20 4904
Fragments arranged by size:
4904 794 532 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6250
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2942 2977 529
Size: 35 2378 35 3802
Fragments arranged by size:
3802 2378 35 35
DraI TTT'AAA
Cuts at: 2890 3554 2890
Size: 664 5586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5257 809
Size: 507 3941 1802
Fragments arranged by size:
3941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2825 3697 4586
Size: 54 1425 50 275 18 995 872 889
Cuts at: 4586 6215 8
Size: 1629 43
Fragments arranged by size:
1629 1425 995 889 872 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2825 3697 1537
Size: 275 1013 872 4090
Fragments arranged by size:
4090 1013 872 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2710 4180 1353
Size: 1357 1470 3423
Fragments arranged by size:
3423 1470 1357
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2112 4837 5211 5357 387
Size: 1064 138 523 2725 374 146 1280
Fragments arranged by size:
2725 1280 1064 523 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2672 2972 3326 3356 4313 4910
Size: 393 612 428 300 354 30 957 597
Cuts at: 4910 5697 5965 1239
Size: 787 268 1524
Fragments arranged by size:
1524 957 787 612 597 428 393 354
300 268 30
EcoO109I rG'GnC_Cy
Cuts at: 2108 2222 2234 2992 3388 2108
Size: 114 12 758 396 4970
Fragments arranged by size:
4970 758 396 114 12
EcoRI G'AATT_C
Cuts at: 3217 3559 3937 3217
Size: 342 378 5530
Fragments arranged by size:
5530 378 342
EcoRV GAT'ATC
Cuts at: 2506 2506
Size: 6250
FspI TGC'GCA
Cuts at: 4756 4756
Size: 6250
HaeII r_GCGC'y
Cuts at: 1678 2052 3436 5397 1678
Size: 374 1384 1961 2531
Fragments arranged by size:
2531 1961 1384 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3244 3276 4891 4923 4965
Size: 32 135 32 1672 32 1615 32 42
Cuts at: 4965 4997 1373
Size: 32 2626
Fragments arranged by size:
2626 1672 1615 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3142 3594 3840 669
Size: 988 281 1204 452 246 3079
Fragments arranged by size:
3079 1204 988 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6250
HpaI GTT'AAC
Cuts at: 3840 3840
Size: 6250
KasI G'GCGC_C
Cuts at: 2048 2048
Size: 6250
KpnI G_GTAC'C
Cuts at: 2429 3575 2429
Size: 1146 5104
MfeI C'AATT_G
Cuts at: 3849 3849
Size: 6250
MluI A'CGCG_T
Cuts at: 6126 6126
Size: 6250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 5364 5548 1305
Size: 985 3074 184 2007
Fragments arranged by size:
3074 2007 985 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4428
Fragments arranged by size:
4428 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4208 4567 4726 518
Size: 648 3042 359 159 2042
Fragments arranged by size:
3042 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3082 3304 3316 3666 4343 5491 5736 1606
Size: 1476 222 12 350 677 1148 245 2120
Fragments arranged by size:
2120 1476 1148 677 350 245 222 12
NarI GG'CG_CC
Cuts at: 2049 2049
Size: 6250
NcoI C'CATG_G
Cuts at: 513 1065 2004 2229 513
Size: 552 939 225 4534
Fragments arranged by size:
4534 939 552 225
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6250
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6250
NotI GC'GGCC_GC
Cuts at: 3697 3697
Size: 6250
NspI r_CATG'y
Cuts at: 1132 1229 3608 5153 1132
Size: 97 2379 1545 2229
Fragments arranged by size:
2379 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3459 3532 1348
Size: 2111 73 4066
Fragments arranged by size:
4066 2111 73
PciI A'CATG_T
Cuts at: 5149 5149
Size: 6250
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2032 1108
Size: 924 5326
PfoI T'CCnGG_A
Cuts at: 2649 2988 2649
Size: 339 5911
PmeI GTTT'AAAC
Cuts at: 3554 3554
Size: 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 5832 5864 4319
Size: 32 1481 32 4705
Fragments arranged by size:
4705 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2222 2234 2992 3388 2222
Size: 12 758 396 5084
Fragments arranged by size:
5084 758 396 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6250
PsiI TTA'TAA
Cuts at: 3820 3820
Size: 6250
PspOMI G'GGCC_C
Cuts at: 2089 2125 2089
Size: 36 6214
PstI C_TGCA'G
Cuts at: 830 3036 3602 830
Size: 2206 566 3478
Fragments arranged by size:
3478 2206 566
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6250
PvuII CAG'CTG
Cuts at: 3304 3304
Size: 6250
SalI G'TCGA_C
Cuts at: 1936 3592 1936
Size: 1656 4594
SanDI GG'GwC_CC
Cuts at: 2234 2234
Size: 6250
SbfI CC_TGCA'GG
Cuts at: 3602 3602
Size: 6250
ScaI AGT'ACT
Cuts at: 4498 6210 4498
Size: 1712 4538
SexAI A'CCwGG_T
Cuts at: 2185 2185
Size: 6250
SfcI C'TryA_G
Cuts at: 826 1045 3032 3598 4733 5414 5605 826
Size: 219 1987 566 1135 681 191 1471
Fragments arranged by size:
1987 1471 1135 681 566 219 191
SfoI GGC'GCC
Cuts at: 2050 2050
Size: 6250
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6250
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6250
SmaI CCC'GGG
Cuts at: 1872 2015 2094 3577 1872
Size: 143 79 1483 4545
Fragments arranged by size:
4545 1483 143 79
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3392 4354 5255 5517 820
Size: 197 926 1449 962 901 262 1553
Fragments arranged by size:
1553 1449 962 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4041 493
Size: 3548 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6250
SphI G_CATG'C
Cuts at: 1132 3608 1132
Size: 2476 3774
SrfI GCCC'GGGC
Cuts at: 2015 2094 2015
Size: 79 6171
SspI AAT'ATT
Cuts at: 5 52 4174 5
Size: 47 4122 2081
Fragments arranged by size:
4122 2081 47
StuI AGG'CCT
Cuts at: 1517 2441 2516 1517
Size: 924 75 5251
Fragments arranged by size:
5251 924 75
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2229 2721 3495 3689 513
Size: 552 939 81 144 492 774 194 3074
Fragments arranged by size:
3074 939 774 552 492 194 144 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2819 4277 4294 4447 4632 5077 325
Size: 1255 1239 1458 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 1239 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2849 4496 6208
Size: 274 80 33 51 1443 872 1647 1712
Cuts at: 6208 96
Size: 138
Fragments arranged by size:
1712 1647 1443 872 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2560 2592 2970 3002 3461 3493 6210 6242 2560
Size: 32 378 32 459 32 2717 32 2568
Fragments arranged by size:
2717 2568 459 378 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6250
XbaI T'CTAG_A
Cuts at: 3262 3586 3262
Size: 324 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2804 2804
Size: 6250
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6250
XmaI C'CCGG_G
Cuts at: 1870 2013 2092 3575 1870
Size: 143 79 1483 4545
Fragments arranged by size:
4545 1483 143 79
XmnI GAAnn'nnTTC
Cuts at: 4379 4379
Size: 6250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4058 276
Size: 53 83 186 936 2524 2468
Fragments arranged by size:
2524 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SfiI SwaI