Restriction map for
pFN21AB0467
(Circular) (Six-base) MAPSORT of: pFN21AB0467.seq Check: 4144 from: 1 to: 6247
pFN21AB0467 6247 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3419 3419
Size: 6247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3590 1937
Size: 1653 4594
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 3328 4374 4747 1563
Size: 1765 1046 373 3063
Fragments arranged by size:
3063 1765 1046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2777 3341 4310 5694 5962 1632
Size: 1145 564 969 1384 268 1917
Fragments arranged by size:
1917 1384 1145 969 564 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 5146 6123 1203
Size: 3943 977 1327
Fragments arranged by size:
3943 1327 977
AgeI A'CCGG_T
Cuts at: 3544 5078 3544
Size: 1534 4713
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2755 3990 5562 2755
Size: 1235 1572 3440
Fragments arranged by size:
3440 1572 1235
ApaI G_GGCC'C
Cuts at: 2051 2956 2051
Size: 905 5342
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 3080 3310 3556 3782 3934 6043 1493
Size: 1587 230 246 226 152 2109 1697
Fragments arranged by size:
2109 1697 1587 246 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 3572 1870
Size: 73 1629 4545
Fragments arranged by size:
4545 1629 73
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 3568 5023 618
Size: 325 217 627 237 1544 1455 1842
Fragments arranged by size:
1842 1544 1455 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2051 2496 2956 3566 1391
Size: 660 445 460 610 4072
Fragments arranged by size:
4072 660 610 460 445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2631 3108 928
Size: 971 732 477 4067
Fragments arranged by size:
4067 971 732 477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2734 2768 4438 4472 1903
Size: 34 797 34 1670 34 3678
Fragments arranged by size:
3678 1670 797 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2660 4858 136
Size: 107 122 71 631 605 988 2198 1525
Fragments arranged by size:
2198 1525 988 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 2103 2573 3664 2103
Size: 470 1091 4686
Fragments arranged by size:
4686 1091 470
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2956 4308 5464 1790
Size: 261 905 1352 1156 2573
Fragments arranged by size:
2573 1352 1156 905 261
BmgBI CAC'GTC
Cuts at: 1318 3053 1318
Size: 1735 4512
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 3978 4936
Size: 416 53 83 399 24 2108 453 958
Cuts at: 4936 442
Size: 1753
Fragments arranged by size:
2108 1753 958 453 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4907 1239
Size: 3668 2579
Bpu10I CC'TnA_GC
Cuts at: 3069 3069
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4910 882
Size: 617 3411 2219
Fragments arranged by size:
3411 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2309 4038 493
Size: 711 1105 1729 2702
Fragments arranged by size:
2702 1729 1105 711
BsaBI GATnn'nnATC
Cuts at: 3365 6241 3365
Size: 2876 3371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 4054 4436 5071
Size: 53 83 186 936 1789 732 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1789 1451 936 732 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3544 4680 5078 5352 5499 5910 3544
Size: 1136 398 274 147 411 3881
Fragments arranged by size:
3881 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3461 3491 1178
Size: 30 165 30 2058 30 3934
Fragments arranged by size:
3934 2058 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 2504 2552 2582 2680 2780 1186
Size: 1204 114 48 30 98 100 4653
Fragments arranged by size:
4653 1204 114 100 98 48 30
BseYI C'CCAG_C
Cuts at: 5450 5946 5450
Size: 496 5751
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5674
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2109 2496 4308 4393 5464 2109
Size: 387 1812 85 1071 2892
Fragments arranged by size:
2892 1812 1071 387 85
BsmI GAATG_Cn'
Cuts at: 2713 3758 3851 2713
Size: 1045 93 5109
Fragments arranged by size:
5109 1045 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2910 3043 2910
Size: 133 6114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2109 2496 2956 3566 4308 4393
Size: 399 261 58 387 460 610 742 85
Cuts at: 4393 5464 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 610 460 399 387 261
85 58
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3419 3602 844
Size: 2575 183 3489
Fragments arranged by size:
3489 2575 183
BsrBI CCG'CTC
Cuts at: 1394 1823 2179 2542 4134 1394
Size: 429 356 363 1592 3507
Fragments arranged by size:
3507 1592 429 363 356
BsrDI GCAATG_nn'
Cuts at: 65 1248 4742 4916 65
Size: 1183 3494 174 1396
Fragments arranged by size:
3494 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3544 4891 5078 1700
Size: 110 1734 1347 187 2869
Fragments arranged by size:
2869 1734 1347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 3188 96
Size: 1911 1181 3155
Fragments arranged by size:
3155 1911 1181
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 2942 4307 5319 2942
Size: 1365 1012 3870
Fragments arranged by size:
3870 1365 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2555 3525 794
Size: 1761 970 3516
Fragments arranged by size:
3516 1761 970
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstXI CCAn_nnnn'nTGG
Cuts at: 2021 2021
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2075 3577 3944 4329 4346 5798 5884 5896
Size: 103 1502 367 385 17 1452 86 12
Cuts at: 5896 6242 1972
Size: 346 1977
Fragments arranged by size:
1977 1502 1452 385 367 346 103 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 3763 4557 4577 3763
Size: 794 20 5433
Fragments arranged by size:
5433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212
Size: 54 1425 50 275 18 1864 889 1629
Cuts at: 6212 8
Size: 43
Fragments arranged by size:
1864 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2589 2757 2967 3294 3399 4177 1353
Size: 1236 168 210 327 105 778 3423
Fragments arranged by size:
3423 1236 778 327 210 168 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4834 5208 5354 387
Size: 1064 138 3245 374 146 1280
Fragments arranged by size:
3245 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2494 2494
Size: 6247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2777 3341 4310 4907 5694 5962 1239
Size: 393 1145 564 969 597 787 268 1524
Fragments arranged by size:
1524 1145 969 787 597 564 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2088 2088
Size: 6247
EcoO109I rG'GnC_Cy
Cuts at: 2047 2048 2952 2953 3472 2047
Size: 1 904 1 519 4822
Fragments arranged by size:
4822 904 519 1 1
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FspI TGC'GCA
Cuts at: 3518 4753 3518
Size: 1235 5012
HaeII r_GCGC'y
Cuts at: 1678 5394 1678
Size: 3716 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 3461 3493 4888
Size: 32 135 32 663 32 1194 32 1395
Cuts at: 4888 4920 4962 4994 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 1194 663 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3536 748
Size: 2788 3459
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 2354 3846 2354
Size: 1492 4755
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 5361 5545 1305
Size: 844 3212 184 2007
Fragments arranged by size:
3212 2007 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4425
Fragments arranged by size:
4425 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2811 3270 4205 4564 4723 518
Size: 648 1645 459 935 359 159 2042
Fragments arranged by size:
2042 1645 935 648 459 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2035 2752 3663 4340 5488 5733 1606
Size: 411 18 717 911 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 717 677 411 245 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5695
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3528 3605 5150 1132
Size: 97 2299 77 1545 2229
Fragments arranged by size:
2299 2229 1545 97 77
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 5146 5146
Size: 6247
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2616 1108
Size: 1508 4739
PfoI T'CCnGG_A
Cuts at: 3465 3465
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2620 4316 4348 5829 5861 2588
Size: 32 1696 32 1481 32 2974
Fragments arranged by size:
2974 1696 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3472 3472
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PspOMI G'GGCC_C
Cuts at: 2047 2952 2047
Size: 905 5342
PstI C_TGCA'G
Cuts at: 830 3599 830
Size: 2769 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2752 2752
Size: 6247
SacI G_AGCT'C
Cuts at: 2496 2496
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SapI GCTCTTCn'nnn_
Cuts at: 2589 2589
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 3595 4730 5411 5602 826
Size: 219 2550 1135 681 191 1471
Fragments arranged by size:
2550 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3528 3605 1132
Size: 2396 77 3774
Fragments arranged by size:
3774 2396 77
SspI AAT'ATT
Cuts at: 5 52 3038 3093 4171 5
Size: 47 2986 55 1078 2081
Fragments arranged by size:
2986 2081 1078 55 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6247
StyI C'CwwG_G
Cuts at: 513 1065 2095 2637 3686 513
Size: 552 1030 542 1049 3074
Fragments arranged by size:
3074 1049 1030 552 542
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2643 3497 4274 4291 4444 4629 5074
Size: 1255 1063 854 777 17 153 185 445
Cuts at: 5074 325
Size: 1498
Fragments arranged by size:
1498 1255 1063 854 777 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3188 3342
Size: 274 80 33 51 1443 30 1181 154
Cuts at: 3342 4493 6205 96
Size: 1151 1712 138
Fragments arranged by size:
1712 1443 1181 1151 274 154 138 80
51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6207 6239 6207
Size: 32 6215
Tth111I GACn'n_nGTC
Cuts at: 1316 3461 1316
Size: 2145 4102
XbaI T'CTAG_A
Cuts at: 2113 3583 2113
Size: 1470 4777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 2898 4376 2898
Size: 1478 4769
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI