Restriction map for
pFN21AB0461
(Circular) (Six-base) MAPSORT of: pFN21AB0461.seq Check: 882 from: 1 to: 6034
pFN21AB0461 6034 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2330 3222 3377 1937
Size: 393 892 155 4594
Fragments arranged by size:
4594 892 393 155
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 3333 4161 4534 1563
Size: 1770 828 373 3063
Fragments arranged by size:
3063 1770 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 3073 3255 4097 5481 5749 1632
Size: 641 800 182 842 1384 268 1917
Fragments arranged by size:
1917 1384 842 800 641 268 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 2568 4933 5910 1203
Size: 1365 2365 977 1327
Fragments arranged by size:
2365 1365 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 2672 2717 1166
Size: 1506 45 4483
Fragments arranged by size:
4483 1506 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 2852
Size: 32 6002
AlwNI CAG_nnn'CTG
Cuts at: 3777 5349 3777
Size: 1572 4462
ApaLI G'TGCA_C
Cuts at: 4091 5247 4091
Size: 1156 4878
ApoI r'AATT_y
Cuts at: 1493 2096 2792 3026 3343 3569 3721 5830 1493
Size: 603 696 234 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 603 317 234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2237 3156 3359 1870
Size: 73 294 919 203 4545
Fragments arranged by size:
4545 919 294 203 73
BamHI G'GATC_C
Cuts at: 2425 3364 3731 2425
Size: 939 367 4728
Fragments arranged by size:
4728 939 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 3355 4810 618
Size: 325 217 627 665 903 1455 1842
Fragments arranged by size:
1842 1455 903 665 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2667 3353 1391
Size: 1276 686 4072
Fragments arranged by size:
4072 1276 686
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5063
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2732 4225 4259 1903
Size: 34 761 34 1493 34 3678
Fragments arranged by size:
3678 1493 761 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 2676 1548
Size: 1128 4906
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4645 136
Size: 107 122 71 631 605 2973 1525
Fragments arranged by size:
2973 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3246 6029 3246
Size: 2783 3251
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 4095 5251 1790
Size: 2305 1156 2573
Fragments arranged by size:
2573 2305 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4694 1239
Size: 3455 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3149 4159 5024 5322 3149
Size: 1010 865 298 3861
Fragments arranged by size:
3861 1010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2458 4697 882
Size: 617 959 2239 2219
Fragments arranged by size:
2239 2219 959 617
BsaAI yAC'GTr
Cuts at: 493 1204 3825 493
Size: 711 2621 2702
Fragments arranged by size:
2702 2621 711
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4467 4865 5139 5286 5697 4467
Size: 398 274 147 411 4804
Fragments arranged by size:
4804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 1178
Size: 30 165 30 1116 30 4663
Fragments arranged by size:
4663 1116 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2423 2675 1186
Size: 838 399 252 4545
Fragments arranged by size:
4545 838 399 252
BseYI C'CCAG_C
Cuts at: 2301 5237 5733 2301
Size: 2936 496 2602
Fragments arranged by size:
2936 2602 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 3484 4245 5273 1540
Size: 275 186 69 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186 69
BsiHKAI G_wGCw'C
Cuts at: 2667 4095 4180 5251 2667
Size: 1428 85 1071 3450
Fragments arranged by size:
3450 1428 1071 85
BsmI GAATG_Cn'
Cuts at: 2314 3545 3638 2314
Size: 1231 93 4710
Fragments arranged by size:
4710 1231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2667 3353 4095 4180 5251 1391
Size: 399 877 686 742 85 1071 2174
Fragments arranged by size:
2174 1071 877 742 686 399 85
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 2892 2968 3063 3923 5653 2892
Size: 76 95 860 1730 3273
Fragments arranged by size:
3273 1730 860 95 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3389 844
Size: 2545 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2704 4529 4703 65
Size: 1183 1456 1825 174 1396
Fragments arranged by size:
1825 1456 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2415 4678 4865 1700
Size: 110 605 2263 187 2869
Fragments arranged by size:
2869 2263 605 187 110
BsrGI T'GTAC_A
Cuts at: 96 2721 96
Size: 2625 3409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 2425 2812 2926 3246 3364 3731 4116 4133
Size: 453 387 114 320 118 367 385 17
Cuts at: 4133 5585 5671 5683 6029 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 453 387 385 367 346 320
118 114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2585 2585
Size: 6034
BtgI C'CryG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4078
Fragments arranged by size:
4078 939 552 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3294 3550 4344 4364 3294
Size: 256 794 20 4964
Fragments arranged by size:
4964 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3481 4370 5999
Size: 54 1425 50 275 18 1651 889 1629
Cuts at: 5999 8
Size: 43
Fragments arranged by size:
1651 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2494 2796 2854 3964 1353
Size: 896 245 302 58 1110 3423
Fragments arranged by size:
3423 1110 896 302 245 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4621 4995 5141 387
Size: 1064 138 3032 374 146 1280
Fragments arranged by size:
3032 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2665 2665
Size: 6034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 3073 3255 4097 4694 5481 5749
Size: 393 641 800 182 842 597 787 268
Cuts at: 5749 1239
Size: 1524
Fragments arranged by size:
1524 842 800 787 641 597 393 268
182
EcoRI G'AATT_C
Cuts at: 2096 3343 3721 2096
Size: 1247 378 4409
Fragments arranged by size:
4409 1247 378
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 5181 1678
Size: 3503 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2164 2196 4675
Size: 32 135 32 497 32 63 32 2479
Cuts at: 4675 4707 4749 4781 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2479 497 135 63 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3223 3331 3378 3624 669
Size: 988 281 610 675 108 47 246 3079
Fragments arranged by size:
3079 988 675 610 281 246 108 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3331 3624 3331
Size: 293 5741
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5148 5332 1305
Size: 3843 184 2007
Fragments arranged by size:
3843 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4212
Fragments arranged by size:
4212 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 2672 2717 3992 4351 4510 518
Size: 648 895 611 45 1275 359 159 2042
Fragments arranged by size:
2042 1275 895 648 611 359 159 45
MspA1I CmG'CkG
Cuts at: 1606 2576 3450 4127 5275 5520 1606
Size: 970 874 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 874 677 245
NaeI GCC'GGC
Cuts at: 2417 2417
Size: 6034
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 513
Size: 552 939 465 4078
Fragments arranged by size:
4078 939 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NgoMIV G'CCGG_C
Cuts at: 2415 2415
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 2572 2761 3392 4937 1132
Size: 97 1343 189 631 1545 2229
Fragments arranged by size:
2229 1545 1343 631 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6034
PciI A'CATG_T
Cuts at: 2568 4933 2568
Size: 2365 3669
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6034
PfoI T'CCnGG_A
Cuts at: 2421 3318 2421
Size: 897 5137
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6034
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PstI C_TGCA'G
Cuts at: 830 3386 830
Size: 2556 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
SacI G_AGCT'C
Cuts at: 2667 2667
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3221 3376 1936
Size: 1285 155 4594
Fragments arranged by size:
4594 1285 155
SapI GCTCTTCn'nnn_
Cuts at: 2796 2796
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SexAI A'CCwGG_T
Cuts at: 2961 2961
Size: 6034
SfcI C'TryA_G
Cuts at: 826 1045 3382 4517 5198 5389 826
Size: 219 2337 1135 681 191 1471
Fragments arranged by size:
2337 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3158 3361 1872
Size: 1286 203 4545
Fragments arranged by size:
4545 1286 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3128 4138 5039 5301 820
Size: 197 926 1185 1010 901 262 1553
Fragments arranged by size:
1553 1185 1010 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 2154 2632 3280 3958 5
Size: 47 2102 478 648 678 2081
Fragments arranged by size:
2102 2081 678 648 478 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3473 513
Size: 552 939 465 1004 3074
Fragments arranged by size:
3074 1004 939 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 4061 4078 4231 4416 4861 325
Size: 1255 504 1977 17 153 185 445 1498
Fragments arranged by size:
1977 1498 1255 504 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 4280 5992
Size: 274 80 33 51 1443 744 1559 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
1712 1559 1443 744 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2519 2551 5994 6026 2519
Size: 32 3443 32 2527
Fragments arranged by size:
3443 2527 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3056 1316
Size: 1740 4294
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 3156 3359 1870
Size: 1286 203 4545
Fragments arranged by size:
4545 1286 203
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI