Restriction map for
pFN21AB0459
(Circular) (Six-base) MAPSORT of: pFN21AB0459.seq Check: 1398 from: 1 to: 6229
pFN21AB0459 6229 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3452 3452
Size: 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2505 4039 278
Size: 53 83 186 936 969 1534 2468
Fragments arranged by size:
2468 1534 969 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 2265 3327 3550 2265
Size: 1062 223 4944
Fragments arranged by size:
4944 1062 223
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4292 5676 5944 1632
Size: 2660 1384 268 1917
Fragments arranged by size:
2660 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 2061 2964 4958 2061
Size: 903 1994 3332
Fragments arranged by size:
3332 1994 903
AleI CACnn'nnGTG
Cuts at: 1166 2286 1166
Size: 1120 5109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2420 2452 2420
Size: 32 6197
AlwNI CAG_nnn'CTG
Cuts at: 2077 2413 2905 2995 3387 3972 5544 2077
Size: 336 492 90 392 585 1572 2762
Fragments arranged by size:
2762 1572 585 492 392 336 90
ApaI G_GGCC'C
Cuts at: 2152 2152
Size: 6229
ApaLI G'TGCA_C
Cuts at: 3053 3365 4286 5442 3053
Size: 312 921 1156 3840
Fragments arranged by size:
3840 1156 921 312
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2152 2389 2423 2961 3185 3344 3444
Size: 73 209 237 34 538 224 159 100
Cuts at: 3444 3554 1870
Size: 110 4545
Fragments arranged by size:
4545 538 237 224 209 159 110 100
73 34
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2156 2265 3181 3327
Size: 325 217 627 319 50 109 916 146
Cuts at: 3327 3464 3550 5005 618
Size: 137 86 1455 1842
Fragments arranged by size:
1842 1455 916 627 325 319 217 146
137 109 86 50
BanII G_rGCy'C
Cuts at: 1391 2087 2152 2974 3277 3402 3528 3548 1391
Size: 696 65 822 303 125 126 20 4072
Fragments arranged by size:
4072 822 696 303 126 125 65 20
BbeI G_GCGC'C
Cuts at: 3185 3185
Size: 6229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3250 928
Size: 971 1351 3907
Fragments arranged by size:
3907 1351 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4420 4454 1903
Size: 34 50 34 2399 34 3678
Fragments arranged by size:
3678 2399 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2140 2466 4840
Size: 107 122 71 631 605 468 326 2374
Cuts at: 4840 136
Size: 1525
Fragments arranged by size:
2374 1525 631 605 468 326 122 107
71
BglII A'GATC_T
Cuts at: 2385 6224 2385
Size: 3839 2390
BlpI GC'TnA_GC
Cuts at: 2357 2988 3646 2357
Size: 631 658 4940
Fragments arranged by size:
4940 658 631
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2159 3057 3369 3469 4290 5446 1790
Size: 362 7 898 312 100 821 1156 2573
Fragments arranged by size:
2573 1156 898 821 362 312 100 7
BmgBI CAC'GTC
Cuts at: 1318 2227 3370 1318
Size: 909 1143 4177
Fragments arranged by size:
4177 1143 909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 3960 4918
Size: 416 53 83 399 24 738 1805 958
Cuts at: 4918 442
Size: 1753
Fragments arranged by size:
1805 1753 958 738 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 3277 2356
Size: 921 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 3056 3250 4889 1239
Size: 1022 795 194 1639 2579
Fragments arranged by size:
2579 1639 1022 795 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3139 4354 5219 5517 3139
Size: 1215 865 298 3851
Fragments arranged by size:
3851 1215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2626 3091 4892 882
Size: 617 1127 465 1801 2219
Fragments arranged by size:
2219 1801 1127 617 465
BsaAI yAC'GTr
Cuts at: 493 1204 2926 3013 4020 493
Size: 711 1722 87 1007 2702
Fragments arranged by size:
2702 1722 1007 711 87
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2502 3182 3492 4036
Size: 53 83 186 936 969 680 310 544
Cuts at: 4036 4418 5053 275
Size: 382 635 1451
Fragments arranged by size:
1451 969 936 680 635 544 382 310
186 83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3311 3341 1178
Size: 30 165 30 1908 30 4066
Fragments arranged by size:
4066 1908 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2681 2684 1186
Size: 1495 3 4731
Fragments arranged by size:
4731 1495 3
BseYI C'CCAG_C
Cuts at: 2145 2312 3387 3469 5432 5928 2145
Size: 167 1075 82 1963 496 2446
Fragments arranged by size:
2446 1963 1075 496 167 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2507 2602 2879 2993 3480 1163
Size: 573 771 95 277 114 487 3912
Fragments arranged by size:
3912 771 573 487 277 114 95
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2664 2841 3679 4440 5468 1540
Size: 275 186 663 177 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 663 275 186 177
BsiHKAI G_wGCw'C
Cuts at: 2184 2725 2925 3057 3369 4290 4375 5446 2184
Size: 541 200 132 312 921 85 1071 2967
Fragments arranged by size:
2967 1071 921 541 312 200 132 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
BsmBI CGTCTCn'nnnn_
Cuts at: 2125 2828 2882 3484 2125
Size: 703 54 602 4870
Fragments arranged by size:
4870 703 602 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2152 2159 2184 2725 2925 2974
Size: 399 297 65 7 25 541 200 49
Cuts at: 2974 3057 3277 3369 3402 3469 3528 3548 4290
Size: 83 220 92 33 67 59 20 742
Cuts at: 4290 4375 5446 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 399 297 220 200
92 85 83 67 65 59 49 33
25 20 7
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 2844 3375 4118 5848 2844
Size: 531 743 1730 3225
Fragments arranged by size:
3225 1730 743 531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2709 3452 3584 844
Size: 1865 743 132 3489
Fragments arranged by size:
3489 1865 743 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 2987 3139 4116 1394
Size: 429 506 658 152 977 3507
Fragments arranged by size:
3507 977 658 506 429 152
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3159 3408 4873 5060 1700
Size: 110 1349 249 1465 187 2869
Fragments arranged by size:
2869 1465 1349 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 2907 96
Size: 2811 3418
BssHII G'CGCG_C
Cuts at: 1925 2456 1925
Size: 531 5698
BssSI C'ACGA_G
Cuts at: 2861 2975 4289 5301 2861
Size: 114 1314 1012 3789
Fragments arranged by size:
3789 1314 1012 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 2230 1184
Size: 1046 5183
BstXI CCAn_nnnn'nTGG
Cuts at: 2288 2288
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 2385 3559 3926 4311 4328 5780 5866 5878
Size: 413 1174 367 385 17 1452 86 12
Cuts at: 5878 6224 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1174 413 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2059 2369 2377 3315 513
Size: 552 994 310 8 938 3427
Fragments arranged by size:
3427 994 938 552 310 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 3745 4539 4559 3463
Size: 282 794 20 5133
Fragments arranged by size:
5133 794 282 20
ClaI AT'CG_AT
Cuts at: 1554 2547 1554
Size: 993 5236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DraIII CAC_nnn'GTG
Cuts at: 2163 2163
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2494 2533 2661
Size: 54 1425 50 275 18 664 39 128
Cuts at: 2661 2676 2784 2838 3015 3066 3102 3126 3410
Size: 15 108 54 177 51 36 24 284
Cuts at: 3410 3429 3676 4565 6194 8
Size: 19 247 889 1629 43
Fragments arranged by size:
1629 1425 889 664 284 275 247 177
128 108 54 54 51 50 43 39
36 24 19 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2661 2838 3676 1537
Size: 275 849 177 838 4090
Fragments arranged by size:
4090 849 838 275 177
EarI CTCTTCn'nnn_
Cuts at: 1353 2026 4159 1353
Size: 673 2133 3423
Fragments arranged by size:
3423 2133 673
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 3056 3250 4292 4889 5676 5944
Size: 393 629 795 194 1042 597 787 268
Cuts at: 5944 1239
Size: 1524
Fragments arranged by size:
1524 1042 795 787 629 597 393 268
194
EcoNI CCTnn'n_nnAGG
Cuts at: 2049 2897 2049
Size: 848 5381
EcoO109I rG'GnC_Cy
Cuts at: 2055 2149 2335 3094 3359 2055
Size: 94 186 759 265 4925
Fragments arranged by size:
4925 759 265 186 94
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2146 2321 2613 3185 5376 1678
Size: 468 175 292 572 2191 2531
Fragments arranged by size:
2531 2191 572 468 292 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2047 2079 2712 2744 2950
Size: 32 135 32 475 32 633 32 206
Cuts at: 2950 2982 4870 4902 4944 4976 1373
Size: 32 1888 32 42 32 2626
Fragments arranged by size:
2626 1888 633 475 206 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3047 748
Size: 2299 3930
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 3181 3181
Size: 6229
KpnI G_GTAC'C
Cuts at: 2269 3331 3554 2269
Size: 1062 223 4944
Fragments arranged by size:
4944 1062 223
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 3288 5343 5527 1305
Size: 1896 87 2055 184 2007
Fragments arranged by size:
2055 2007 1896 184 87
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2678 3104 3128 10
Size: 54 1425 343 846 426 24 3111
Fragments arranged by size:
3111 1425 846 426 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2579 2915 4187 4546 4705 518
Size: 648 1120 293 336 1272 359 159 2042
Fragments arranged by size:
2042 1272 1120 648 359 336 293 159
MspA1I CmG'CkG
Cuts at: 1606 2312 2413 3384 3645 4322 5470 5715 1606
Size: 706 101 971 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 706 677 261 245 101
NaeI GCC'GGC
Cuts at: 3161 3410 3161
Size: 249 5980
NarI GG'CG_CC
Cuts at: 3182 3182
Size: 6229
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 3265 387
Size: 2878 3351
NgoMIV G'CCGG_C
Cuts at: 3159 3408 3159
Size: 249 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6229
PfoI T'CCnGG_A
Cuts at: 2238 2826 2238
Size: 588 5641
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PmlI CAC'GTG
Cuts at: 2926 3013 2926
Size: 87 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 4298 4330 5811 5843 2543
Size: 32 1723 32 1481 32 2929
Fragments arranged by size:
2929 1723 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 3094 2055
Size: 1039 5190
PshAI GACnn'nnGTC
Cuts at: 1613 2840 1613
Size: 1227 5002
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2148 2148
Size: 6229
PstI C_TGCA'G
Cuts at: 830 2418 2706 2760 3000 3581 830
Size: 1588 288 54 240 581 3478
Fragments arranged by size:
3478 1588 581 288 240 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2312 2413 3384 2312
Size: 101 971 5157
Fragments arranged by size:
5157 971 101
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SanDI GG'GwC_CC
Cuts at: 2055 2055
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 2760 3581 2760
Size: 821 5408
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SexAI A'CCwGG_T
Cuts at: 2868 2940 3096 2868
Size: 72 156 6001
Fragments arranged by size:
6001 156 72
SfcI C'TryA_G
Cuts at: 826 1045 2414 2702 2756 2996 3577 4712 5393
Size: 219 1369 288 54 240 581 1135 681
Cuts at: 5393 5584 826
Size: 191 1471
Fragments arranged by size:
1471 1369 1135 681 581 288 240 219
191 54
SfoI GGC'GCC
Cuts at: 3183 3183
Size: 6229
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2154 2963 3446 3556 1872
Size: 282 809 483 110 4545
Fragments arranged by size:
4545 809 483 282 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 4333 5234 5496 820
Size: 197 926 1211 1179 901 262 1553
Fragments arranged by size:
1553 1211 1179 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 2246 2822 1517
Size: 729 576 4924
Fragments arranged by size:
4924 729 576
StyI C'CwwG_G
Cuts at: 513 1065 3105 3668 513
Size: 552 2040 563 3074
Fragments arranged by size:
3074 2040 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 4256 4273 4426 4611 5056 325
Size: 1255 594 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 594 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2907 4475 6187
Size: 274 80 33 51 1443 930 1568 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1568 1443 930 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2474 2656 1316
Size: 912 246 182 4889
Fragments arranged by size:
4889 912 246 182
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2142 2142
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2152 2961 3444 3554 1870
Size: 282 809 483 110 4545
Fragments arranged by size:
4545 809 483 282 110
XmnI GAAnn'nnTTC
Cuts at: 4358 4358
Size: 6229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2503 4037 276
Size: 53 83 186 936 969 1534 2468
Fragments arranged by size:
2468 1534 969 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SapI
SfiI SrfI SwaI