Restriction map for
pFN21AB0458
(Circular) (Six-base) MAPSORT of: pFN21AB0458.seq Check: 5006 from: 1 to: 7552
pFN21AB0458 7552 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2903 2903
Size: 7552
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5362 278
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4895 1937
Size: 2958 4594
Acc65I G'GTAC_C
Cuts at: 4873 4873
Size: 7552
AclI AA'CG_TT
Cuts at: 1563 5679 6052 1563
Size: 4116 373 3063
Fragments arranged by size:
4116 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2814 2874 3929 3968 4658 5615 6999 7267
Size: 1182 60 1055 39 690 957 1384 268
Cuts at: 7267 1632
Size: 1917
Fragments arranged by size:
1917 1384 1182 1055 957 690 268 60
39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7355
AflIII A'CryG_T
Cuts at: 1203 2163 3341 6451 7428 1203
Size: 960 1178 3110 977 1327
Fragments arranged by size:
3110 1327 1178 977 960
AgeI A'CCGG_T
Cuts at: 6383 6383
Size: 7552
AhdI GACnn_n'nnGTC
Cuts at: 6281 6281
Size: 7552
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2257 4393 1184
Size: 1073 2136 4343
Fragments arranged by size:
4343 2136 1073
AlwNI CAG_nnn'CTG
Cuts at: 2696 5295 6867 2696
Size: 2599 1572 3381
Fragments arranged by size:
3381 2599 1572
ApaLI G'TGCA_C
Cuts at: 4586 5609 6765 4586
Size: 1023 1156 5373
Fragments arranged by size:
5373 1156 1023
ApoI r'AATT_y
Cuts at: 1493 2884 2902 3032 3206 3721 4499 4670 4861
Size: 1391 18 130 174 515 778 171 191
Cuts at: 4861 5087 5239 7348 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 778 515 226 191 174
171 152 130 18
AseI AT'TA_AT
Cuts at: 160 1032 6106 160
Size: 872 5074 1606
Fragments arranged by size:
5074 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
AvaI C'yCGr_G
Cuts at: 1870 1943 2305 3127 3521 4053 4877 1870
Size: 73 362 822 394 532 824 4545
Fragments arranged by size:
4545 824 822 532 394 362 73
AvrII C'CTAG_G
Cuts at: 2554 2554
Size: 7552
BamHI G'GATC_C
Cuts at: 4251 4882 5249 4251
Size: 631 367 6554
Fragments arranged by size:
6554 631 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 3754 3863 4873 6328
Size: 325 217 627 602 1365 109 1010 1455
Cuts at: 6328 618
Size: 1842
Fragments arranged by size:
1842 1455 1365 1010 627 602 325 217
109
BanII G_rGCy'C
Cuts at: 1391 3243 3483 4158 4241 4599 4871 1391
Size: 1852 240 675 83 358 272 4072
Fragments arranged by size:
4072 1852 675 358 272 240 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4646 928
Size: 971 2747 3834
Fragments arranged by size:
3834 2747 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5743 5777 1903
Size: 34 3806 34 3678
Fragments arranged by size:
3806 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5444 6654 5444
Size: 1210 6342
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7552
BfrBI ATG'CAT
Cuts at: 4288 4288
Size: 7552
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6163 136
Size: 107 122 71 631 605 4491 1525
Fragments arranged by size:
4491 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2923 3786 7547 2923
Size: 863 3761 2928
Fragments arranged by size:
3761 2928 863
BlpI GC'TnA_GC
Cuts at: 4969 4969
Size: 7552
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4590 5613 6769 1790
Size: 1969 831 1023 1156 2573
Fragments arranged by size:
2573 1969 1156 1023 831
BmgBI CAC'GTC
Cuts at: 1318 2164 4012 4021 1318
Size: 846 1848 9 4849
Fragments arranged by size:
4849 1848 846 9
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3682 4801 4816
Size: 416 53 83 399 24 2265 1119 15
Cuts at: 4816 5283 6241 442
Size: 467 958 1753
Fragments arranged by size:
2265 1753 1119 958 467 416 399 83
53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7552
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 7552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4583 6212 1239
Size: 3344 1629 2579
Fragments arranged by size:
3344 2579 1629
Bpu10I CC'TnA_GC
Cuts at: 3482 3482
Size: 7552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3257 4475 5677 6542 6840 3257
Size: 1218 1202 865 298 3969
Fragments arranged by size:
3969 1218 1202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2412 4251 6215 882
Size: 617 552 361 1839 1964 2219
Fragments arranged by size:
2219 1964 1839 617 552 361
BsaAI yAC'GTr
Cuts at: 493 1204 3617 5343 493
Size: 711 2413 1726 2702
Fragments arranged by size:
2702 2413 1726 711
BsaBI GATnn'nnATC
Cuts at: 7546 7546
Size: 7552
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3394 5359 5741 6376
Size: 53 83 186 936 1861 1965 382 635
Cuts at: 6376 275
Size: 1451
Fragments arranged by size:
1965 1861 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2449 3973 5985 6383 6657 6804 7215 2449
Size: 1524 2012 398 274 147 411 2786
Fragments arranged by size:
2786 2012 1524 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3293 3323 4124 4154 1178
Size: 30 165 30 1890 30 801 30 4576
Fragments arranged by size:
4576 1890 801 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 3190 4607 1186
Size: 855 1149 1417 4131
Fragments arranged by size:
4131 1417 1149 855
BseYI C'CCAG_C
Cuts at: 2572 3593 3868 6755 7251 2572
Size: 1021 275 2887 496 2873
Fragments arranged by size:
2887 2873 1021 496 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2459 3593 1163
Size: 573 723 1134 5122
Fragments arranged by size:
5122 1134 723 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5002 5763 6791 1540
Size: 275 186 3001 761 1028 2301
Fragments arranged by size:
3001 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3243 3991 4241 4590 4599 4748 5613 5698 6769
Size: 748 250 349 9 149 865 85 1071
Cuts at: 6769 3243
Size: 4026
Fragments arranged by size:
4026 1071 865 748 349 250 149 85
9
BsmI GAATG_Cn'
Cuts at: 3450 5063 5156 3450
Size: 1613 93 5846
Fragments arranged by size:
5846 1613 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3243 3483 3759 3991 4158 4241 4590
Size: 399 1453 240 276 232 167 83 349
Cuts at: 4590 4599 4748 4871 5613 5698 6769 1391
Size: 9 149 123 742 85 1071 2174
Fragments arranged by size:
2174 1453 1071 742 399 349 276 240
232 167 149 123 85 83 9
BspEI T'CCGG_A
Cuts at: 2449 3973 7215 2449
Size: 1524 3242 2786
Fragments arranged by size:
3242 2786 1524
BspHI T'CATG_A
Cuts at: 5441 7171 5441
Size: 1730 5822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2287 2903 4626 4907 844
Size: 1443 616 1723 281 3489
Fragments arranged by size:
3489 1723 1443 616 281
BsrBI CCG'CTC
Cuts at: 1394 1823 5439 1394
Size: 429 3616 3507
Fragments arranged by size:
3616 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2782 4628 4714 6047 6221 65
Size: 1183 1534 1846 86 1333 174 1396
Fragments arranged by size:
1846 1534 1396 1333 1183 174 86
BsrFI r'CCGG_y
Cuts at: 1700 1810 6196 6383 1700
Size: 110 4386 187 2869
Fragments arranged by size:
4386 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7552
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7552
BssSI C'ACGA_G
Cuts at: 2081 3464 4157 4589 5612 6624 2081
Size: 1383 693 432 1023 1012 3009
Fragments arranged by size:
3009 1383 1023 1012 693 432
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7552
BstBI TT'CG_AA
Cuts at: 4176 6356 4176
Size: 2180 5372
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7552
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2821 3691 2068
Size: 753 870 5929
Fragments arranged by size:
5929 870 753
BstYI r'GATC_y
Cuts at: 1972 2606 2722 2923 3180 3786 4251 4882 5249
Size: 634 116 201 257 606 465 631 367
Cuts at: 5249 5634 5651 7103 7189 7201 7547 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 634 631 606 465 385 367
346 257 201 116 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2371 2371
Size: 7552
BtgI C'CryG_G
Cuts at: 513 1065 2004 2661 3706 513
Size: 552 939 657 1045 4359
Fragments arranged by size:
4359 1045 939 657 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2128 3887 4470 4828 487
Size: 683 56 902 1759 583 358 3211
Fragments arranged by size:
3211 1759 902 683 583 358 56
BtsI GCAGTG_nn'
Cuts at: 2025 2154 2340 5068 5862 5882 2025
Size: 129 186 2728 794 20 3695
Fragments arranged by size:
3695 2728 794 186 129 20
ClaI AT'CG_AT
Cuts at: 1554 3657 1554
Size: 2103 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3033 3068 3278 3313 529
Size: 35 2469 35 210 35 4768
Fragments arranged by size:
4768 2469 210 35 35 35
DraI TTT'AAA
Cuts at: 4856 4856
Size: 7552
DraIII CAC_nnn'GTG
Cuts at: 2624 3516 3986 2624
Size: 892 470 6190
Fragments arranged by size:
6190 892 470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2168 2565 6559 809
Size: 507 852 397 3994 1802
Fragments arranged by size:
3994 1802 852 507 397
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 3693 4999
Size: 54 1425 50 275 18 285 1578 1306
Cuts at: 4999 5888 7517 8
Size: 889 1629 43
Fragments arranged by size:
1629 1578 1425 1306 889 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4999 1537
Size: 275 3187 4090
Fragments arranged by size:
4090 3187 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 3800 5482 1353
Size: 684 1763 1682 3423
Fragments arranged by size:
3423 1763 1682 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6139 6513 6659 387
Size: 1064 138 4550 374 146 1280
Fragments arranged by size:
4550 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3241 4239 4597 3241
Size: 998 358 6196
Fragments arranged by size:
6196 998 358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2814 2874 3929 3968 4583 4658 5615
Size: 393 1182 60 1055 39 615 75 957
Cuts at: 5615 6212 6999 7267 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1182 1055 957 787 615 597 393
268 75 60 39
EcoO109I rG'GnC_Cy
Cuts at: 2848 3145 3586 2848
Size: 297 441 6814
Fragments arranged by size:
6814 441 297
EcoRI G'AATT_C
Cuts at: 4499 4670 4861 5239 4499
Size: 171 191 378 6812
Fragments arranged by size:
6812 378 191 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2778 3511 2778
Size: 733 6819
FspI TGC'GCA
Cuts at: 4720 6058 4720
Size: 1338 6214
HaeII r_GCGC'y
Cuts at: 1678 6699 1678
Size: 5021 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4273 4305 4393 4425 6193
Size: 32 135 32 2701 32 88 32 1768
Cuts at: 6193 6225 6267 6299 1373
Size: 32 42 32 2626
Fragments arranged by size:
2701 2626 1768 135 88 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4276 4896 5142 669
Size: 988 281 2338 620 246 3079
Fragments arranged by size:
3079 2338 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 3458 748
Size: 2710 4842
HpaI GTT'AAC
Cuts at: 5142 5142
Size: 7552
KpnI G_GTAC'C
Cuts at: 4877 4877
Size: 7552
MfeI C'AATT_G
Cuts at: 3045 5151 3045
Size: 2106 5446
MluI A'CGCG_T
Cuts at: 7428 7428
Size: 7552
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 3362 3578 6666 6850 1305
Size: 850 1207 216 3088 184 2007
Fragments arranged by size:
3088 2007 1207 850 216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2117 3695 10
Size: 54 1425 343 285 1578 3867
Fragments arranged by size:
3867 1578 1425 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2645 3368 4019 4283 4340 5510
Size: 648 981 498 723 651 264 57 1170
Cuts at: 5510 5869 6028 518
Size: 359 159 2042
Fragments arranged by size:
2042 1170 981 723 651 648 498 359
264 159 57
MspA1I CmG'CkG
Cuts at: 1606 2572 2821 3194 4765 4968 5645 6793 7038
Size: 966 249 373 1571 203 677 1148 245
Cuts at: 7038 1606
Size: 2120
Fragments arranged by size:
2120 1571 1148 966 677 373 249 245
203
NcoI C'CATG_G
Cuts at: 513 1065 2004 2661 513
Size: 552 939 657 5404
Fragments arranged by size:
5404 939 657 552
NdeI CA'TA_TG
Cuts at: 387 3350 387
Size: 2963 4589
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7552
NotI GC'GGCC_GC
Cuts at: 4999 4999
Size: 7552
NsiI A_TGCA'T
Cuts at: 4290 4290
Size: 7552
NspI r_CATG'y
Cuts at: 1132 1229 3124 3345 3367 4910 6455 1132
Size: 97 1895 221 22 1543 1545 2229
Fragments arranged by size:
2229 1895 1545 1543 221 97 22
PasI CC'CwG_GG
Cuts at: 1348 2582 1348
Size: 1234 6318
PciI A'CATG_T
Cuts at: 3341 6451 3341
Size: 3110 4442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3436 4015 4248 1108
Size: 2328 579 233 4412
Fragments arranged by size:
4412 2328 579 233
PmeI GTTT'AAAC
Cuts at: 4856 4856
Size: 7552
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5621 5653 7134 7166 5621
Size: 32 1481 32 6007
Fragments arranged by size:
6007 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2848 3586 2848
Size: 738 6814
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7552
PsiI TTA'TAA
Cuts at: 5122 5122
Size: 7552
PstI C_TGCA'G
Cuts at: 830 2911 4904 830
Size: 2081 1993 3478
Fragments arranged by size:
3478 2081 1993
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7552
PvuII CAG'CTG
Cuts at: 2572 3194 4765 2572
Size: 622 1571 5359
Fragments arranged by size:
5359 1571 622
SacI G_AGCT'C
Cuts at: 3243 4241 4599 3243
Size: 998 358 6196
Fragments arranged by size:
6196 998 358
SalI G'TCGA_C
Cuts at: 1936 4894 1936
Size: 2958 4594
SapI GCTCTTCn'nnn_
Cuts at: 3800 3800
Size: 7552
SbfI CC_TGCA'GG
Cuts at: 4904 4904
Size: 7552
ScaI AGT'ACT
Cuts at: 5800 7512 5800
Size: 1712 5840
SfcI C'TryA_G
Cuts at: 826 1045 2907 3076 3579 4900 6035 6716 6907
Size: 219 1862 169 503 1321 1135 681 191
Cuts at: 6907 826
Size: 1471
Fragments arranged by size:
1862 1471 1321 1135 681 503 219 191
169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7552
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7552
SmaI CCC'GGG
Cuts at: 1872 3129 4055 4879 1872
Size: 1257 926 824 4545
Fragments arranged by size:
4545 1257 926 824
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 4490 5656 6557 6819 820
Size: 197 926 1293 1254 1166 901 262 1553
Fragments arranged by size:
1553 1293 1254 1166 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5343 493
Size: 4850 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7552
SphI G_CATG'C
Cuts at: 1132 3124 4910 1132
Size: 1992 1786 3774
Fragments arranged by size:
3774 1992 1786
SspI AAT'ATT
Cuts at: 5 52 3334 5476 5
Size: 47 3282 2142 2081
Fragments arranged by size:
3282 2142 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7552
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2554 2661 4182 4315 4991
Size: 552 939 120 430 107 1521 133 676
Cuts at: 4991 513
Size: 3074
Fragments arranged by size:
3074 1521 939 676 552 430 133 120
107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 3701 4511 5579 5596 5749 5934
Size: 1255 984 1137 810 1068 17 153 185
Cuts at: 5934 6379 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1137 1068 984 810 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3325 4122 4834
Size: 274 80 33 51 1443 1348 797 712
Cuts at: 4834 5798 7510 96
Size: 964 1712 138
Fragments arranged by size:
1712 1443 1348 964 797 712 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 3502 3534 4124 4156 4808 4840 7512
Size: 32 720 32 590 32 652 32 2672
Cuts at: 7512 7544 2750
Size: 32 2758
Fragments arranged by size:
2758 2672 720 652 590 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4010 1316
Size: 2694 4858
XbaI T'CTAG_A
Cuts at: 4888 4888
Size: 7552
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 4432 2131
Size: 2301 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7552
XmaI C'CCGG_G
Cuts at: 1870 3127 4053 4877 1870
Size: 1257 926 824 4545
Fragments arranged by size:
4545 1257 926 824
XmnI GAAnn'nnTTC
Cuts at: 3741 3818 5681 3741
Size: 77 1863 5612
Fragments arranged by size:
5612 1863 77
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5360 276
Size: 53 83 186 936 3826 2468
Fragments arranged by size:
3826 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BsiWI
BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI