Restriction map for
pFN21AB0457
(Circular) (Six-base) MAPSORT of: pFN21AB0457.seq Check: 3334 from: 1 to: 6220
pFN21AB0457 6220 bp
With 182 enzymes: SgfI *
October 20, 2009 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3443 3443
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 2892 3541 2892
Size: 649 5571
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2657 4283 5667 5935 1632
Size: 391 634 1626 1384 268 1917
Fragments arranged by size:
1917 1626 1384 634 391 268
AfeI AGC'GCT
Cuts at: 3367 3367
Size: 6220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 5119 6096 1203
Size: 3916 977 1327
Fragments arranged by size:
3916 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 2243 2777 1166
Size: 1077 534 4609
Fragments arranged by size:
4609 1077 534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2998 3350 3360 3507 3963 5535 2998
Size: 352 10 147 456 1572 3683
Fragments arranged by size:
3683 1572 456 352 147 10
ApaI G_GGCC'C
Cuts at: 2223 2223
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2032 2032
Size: 6220
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3297 3545 1870
Size: 73 1354 248 4545
Fragments arranged by size:
4545 1354 248 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2892 3122 3225 3541 4996
Size: 325 217 627 1105 230 103 316 1455
Cuts at: 4996 618
Size: 1842
Fragments arranged by size:
1842 1455 1105 627 325 316 230 217
103
BanII G_rGCy'C
Cuts at: 1391 2223 2463 3464 3539 1391
Size: 832 240 1001 75 4072
Fragments arranged by size:
4072 1001 832 240 75
BbeI G_GCGC'C
Cuts at: 3126 3229 3126
Size: 103 6117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 2928 3159 1548
Size: 1380 231 4609
Fragments arranged by size:
4609 1380 231
BfrBI ATG'CAT
Cuts at: 2235 2235
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3053 4831 136
Size: 107 122 71 631 605 1381 1778 1525
Fragments arranged by size:
1778 1525 1381 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2223 4281 5437 1790
Size: 433 2058 1156 2573
Fragments arranged by size:
2573 2058 1156 433
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 3052 3951
Size: 416 53 83 399 24 800 835 899
Cuts at: 3951 4909 442
Size: 958 1753
Fragments arranged by size:
1753 958 899 835 800 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2405 2426 2477 2585 2699 2738 3062
Size: 1064 102 21 51 108 114 39 324
Cuts at: 3062 3137 3254 4880 1239
Size: 75 117 1626 2579
Fragments arranged by size:
2579 1626 1064 324 117 114 108 102
75 51 39 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2936 4883 882
Size: 617 1437 1947 2219
Fragments arranged by size:
2219 1947 1437 617
BsaAI yAC'GTr
Cuts at: 493 1204 2551 4011 493
Size: 711 1347 1460 2702
Fragments arranged by size:
2702 1460 1347 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3123 3226 4027 4409
Size: 53 83 186 936 1590 103 801 382
Cuts at: 4409 5044 275
Size: 635 1451
Fragments arranged by size:
1590 1451 936 801 635 382 186 103
83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2489 2519 1178
Size: 30 165 30 1047 30 9 30 4879
Fragments arranged by size:
4879 1047 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2333 2603 2606 2750 2753 2924 2978
Size: 1069 78 270 3 144 3 171 54
Cuts at: 2978 3257 1186
Size: 279 4149
Fragments arranged by size:
4149 1069 279 270 171 144 78 54
3 3
BseYI C'CCAG_C
Cuts at: 3193 5423 5919 3193
Size: 2230 496 3494
Fragments arranged by size:
3494 2230 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2573 1163
Size: 573 837 4810
Fragments arranged by size:
4810 837 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2074 3006 3670 4431 5459 1540
Size: 275 186 73 932 664 761 1028 2301
Fragments arranged by size:
2301 1028 932 761 664 275 186 73
BsiHKAI G_wGCw'C
Cuts at: 3398 4281 4366 5437 3398
Size: 883 85 1071 4181
Fragments arranged by size:
4181 1071 883 85
BsmI GAATG_Cn'
Cuts at: 2748 3022 3731 3824 2748
Size: 274 709 93 5144
Fragments arranged by size:
5144 709 274 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2463 3398 3464 3539 4281 4366
Size: 399 433 240 935 66 75 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 935 742 433 399 240 85
75 66
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2018 2340 3443 3575 844
Size: 1174 322 1103 132 3489
Fragments arranged by size:
3489 1174 1103 322 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2202 4107 1394
Size: 429 379 1905 3507
Fragments arranged by size:
3507 1905 429 379
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4715 4889 65
Size: 1183 1578 1889 174 1396
Fragments arranged by size:
1889 1578 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2171 3398 4864 5051 1700
Size: 110 361 1227 1466 187 2869
Fragments arranged by size:
2869 1466 1227 361 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 2032 1925
Size: 107 6113
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2112 2560 3360 3507 794
Size: 1318 448 800 147 3507
Fragments arranged by size:
3507 1318 800 448 147
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstYI r'GATC_y
Cuts at: 1972 2742 3550 3917 4302 4319 5771 5857 5869
Size: 770 808 367 385 17 1452 86 12
Cuts at: 5869 6215 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 808 770 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2720 2720
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2063 2101 2119 2778 2922 3048 513
Size: 552 998 38 18 659 144 126 3685
Fragments arranged by size:
3685 998 659 552 144 126 38 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2309 3361 3736 4530 4550 2309
Size: 1052 375 794 20 3979
Fragments arranged by size:
3979 1052 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2152 2152
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2858 5227 809
Size: 507 1542 2369 1802
Fragments arranged by size:
2369 1802 1542 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2071 3003 3108
Size: 54 1425 50 275 18 241 932 105
Cuts at: 3108 3341 3418 3667 4556 6185 8
Size: 233 77 249 889 1629 43
Fragments arranged by size:
1629 1425 932 889 275 249 241 233
105 77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2071 3003 3667 1537
Size: 275 259 932 664 4090
Fragments arranged by size:
4090 932 664 275 259
EarI CTCTTCn'nnn_
Cuts at: 1353 4150 1353
Size: 2797 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2303 2405 2426 2477 2585 2657
Size: 393 391 280 102 21 51 108 72
Cuts at: 2657 2699 2738 3062 3137 3254 4283 4880 5667
Size: 42 39 324 75 117 1029 597 787
Cuts at: 5667 5935 1239
Size: 268 1524
Fragments arranged by size:
1524 1029 787 597 393 391 324 280
268 117 108 102 75 72 51 42
39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2702 3131 3233 2468
Size: 234 429 102 5455
Fragments arranged by size:
5455 429 234 102
EcoO109I rG'GnC_Cy
Cuts at: 2695 2806 3319 2695
Size: 111 513 5596
Fragments arranged by size:
5596 513 111
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 3126 3229 3369 5367 1678
Size: 1448 103 140 1998 2531
Fragments arranged by size:
2531 1998 1448 140 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 4861 4893 4935
Size: 32 135 32 1298 32 1959 32 42
Cuts at: 4935 4967 1373
Size: 32 2626
Fragments arranged by size:
2626 1959 1298 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3564 3810 669
Size: 988 281 316 1310 246 3079
Fragments arranged by size:
3079 1310 988 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 3122 3225 3122
Size: 103 6117
KpnI G_GTAC'C
Cuts at: 2896 3545 2896
Size: 649 5571
MfeI C'AATT_G
Cuts at: 3422 3819 3422
Size: 397 5823
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2777 4178 4537 4696 518
Size: 648 1077 534 1401 359 159 2042
Fragments arranged by size:
2042 1401 1077 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2065 2076 2121 2523 2615 3050 3099
Size: 437 22 11 45 402 92 435 49
Cuts at: 3099 3507 3636 4313 5461 5706 1606
Size: 408 129 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 437 435 408 402 245
129 92 49 45 22 11
NaeI GCC'GGC
Cuts at: 2173 2173
Size: 6220
NarI GG'CG_CC
Cuts at: 3123 3226 3123
Size: 103 6117
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2171 2171
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NsiI A_TGCA'T
Cuts at: 2237 2237
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 3578 5123 1132
Size: 97 2349 1545 2229
Fragments arranged by size:
2349 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3481 1348
Size: 2133 4087
PciI A'CATG_T
Cuts at: 5119 5119
Size: 6220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3350 1108
Size: 2242 3978
PfoI T'CCnGG_A
Cuts at: 2382 2703 2382
Size: 321 5899
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PmlI CAC'GTG
Cuts at: 2551 2551
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2806 3319 2806
Size: 513 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2219 2219
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2421 3132 3365 3572 830
Size: 1591 711 233 207 3478
Fragments arranged by size:
3478 1591 711 233 207
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2043 2615 3507 2043
Size: 572 892 4756
Fragments arranged by size:
4756 892 572
SacII CC_GC'GG
Cuts at: 2066 2122 3051 2066
Size: 56 929 5235
Fragments arranged by size:
5235 929 56
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2417 3128 3220 3361 3568 4703 5384
Size: 219 1372 711 92 141 207 1135 681
Cuts at: 5384 5575 826
Size: 191 1471
Fragments arranged by size:
1471 1372 1135 711 681 219 207 191
141 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 3053 3053
Size: 6220
SfoI GGC'GCC
Cuts at: 3124 3227 3124
Size: 103 6117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3299 3547 1872
Size: 1427 248 4545
Fragments arranged by size:
4545 1427 248
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 5225 5487 820
Size: 197 926 2381 901 262 1553
Fragments arranged by size:
2381 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6220
StyI C'CwwG_G
Cuts at: 513 1065 2958 3659 513
Size: 552 1893 701 3074
Fragments arranged by size:
3074 1893 701 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 3473 4247 4264 4417 4602 5047
Size: 1255 659 1234 774 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1498 1255 1234 774 659 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4466 6178 96
Size: 274 80 33 51 1443 2489 1712 138
Fragments arranged by size:
2489 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2489 2521 6180 6212 2489
Size: 32 3659 32 2497
Fragments arranged by size:
3659 2497 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2858 1316
Size: 1542 4678
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3145 3196 3145
Size: 51 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3297 3545 1870
Size: 1427 248 4545
Fragments arranged by size:
4545 1427 248
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI
BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI PacI
PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI
SwaI