Restriction map for
pFN21AB0454
(Circular) (Six-base) MAPSORT of: pFN21AB0454.seq Check: 255 from: 1 to: 7480
pFN21AB0454 7480 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5290 278
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4823 1937
Size: 2886 4594
Acc65I G'GTAC_C
Cuts at: 2883 4801 2883
Size: 1918 5562
AclI AA'CG_TT
Cuts at: 1563 5607 5980 1563
Size: 4044 373 3063
Fragments arranged by size:
4044 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 5543 6927 7195 1632
Size: 1724 2187 1384 268 1917
Fragments arranged by size:
2187 1917 1724 1384 268
AfeI AGC'GCT
Cuts at: 2816 2816
Size: 7480
AflII C'TTAA_G
Cuts at: 820 1017 3407 820
Size: 197 2390 4893
Fragments arranged by size:
4893 2390 197
AflIII A'CryG_T
Cuts at: 1203 6379 7356 1203
Size: 5176 977 1327
Fragments arranged by size:
5176 1327 977
AgeI A'CCGG_T
Cuts at: 6311 6311
Size: 7480
AhdI GACnn_n'nnGTC
Cuts at: 4643 6209 4643
Size: 1566 5914
AleI CACnn'nnGTG
Cuts at: 1166 3196 3626 1166
Size: 2030 430 5020
Fragments arranged by size:
5020 2030 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4623 1184
Size: 3439 4041
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 7448
AlwNI CAG_nnn'CTG
Cuts at: 3176 4462 4567 5223 6795 3176
Size: 1286 105 656 1572 3861
Fragments arranged by size:
3861 1572 1286 656 105
ApaI G_GGCC'C
Cuts at: 2385 2598 3066 3271 2385
Size: 213 468 205 6594
Fragments arranged by size:
6594 468 213 205
ApaLI G'TGCA_C
Cuts at: 3431 4361 5537 6693 3431
Size: 930 1176 1156 4218
Fragments arranged by size:
4218 1176 1156 930
ApoI r'AATT_y
Cuts at: 1493 3864 4510 4789 5015 5167 7276 1493
Size: 2371 646 279 226 152 2109 1697
Fragments arranged by size:
2371 2109 1697 646 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6034 160
Size: 872 5002 1606
Fragments arranged by size:
5002 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2323 2449 4573 4805 1870
Size: 73 72 308 126 2124 232 4545
Fragments arranged by size:
4545 2124 308 232 126 73 72
BamHI G'GATC_C
Cuts at: 2955 3402 4810 5177 2955
Size: 447 1408 367 5258
Fragments arranged by size:
5258 1408 447 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2750 2854 2883 3142 4523
Size: 325 217 627 963 104 29 259 1381
Cuts at: 4523 4801 6256 618
Size: 278 1455 1842
Fragments arranged by size:
1842 1455 1381 963 627 325 278 259
217 104 29
BanII G_rGCy'C
Cuts at: 1391 2250 2336 2385 2510 2598 2828 3066 3271
Size: 859 86 49 125 88 230 238 205
Cuts at: 3271 3519 4059 4209 4283 4388 4574 4799 1391
Size: 248 540 150 74 105 186 225 4072
Fragments arranged by size:
4072 859 540 248 238 230 225 205
186 150 125 105 88 86 74 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2981 3851 928
Size: 971 1082 870 4557
Fragments arranged by size:
4557 1082 971 870
BbvCI CC'TCA_GC
Cuts at: 4014 4014
Size: 7480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2304 2338 5671 5705 1903
Size: 34 367 34 3333 34 3678
Fragments arranged by size:
3678 3333 367 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5372 6582 5372
Size: 1210 6270
BclI T'GATC_A
Cuts at: 1548 2910 1548
Size: 1362 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 3127 4178
Size: 107 122 71 631 605 766 689 1051
Cuts at: 4178 4293 4316 6091 136
Size: 115 23 1775 1525
Fragments arranged by size:
1775 1525 1051 766 689 631 605 122
115 107 71 23
BglII A'GATC_T
Cuts at: 3375 7475 3375
Size: 4100 3380
BlpI GC'TnA_GC
Cuts at: 4387 4568 4897 4387
Size: 181 329 6970
Fragments arranged by size:
6970 329 181
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2472 2598 2721 3066 3271 3435 4365
Size: 595 87 126 123 345 205 164 930
Cuts at: 4365 5541 6697 1790
Size: 1176 1156 2573
Fragments arranged by size:
2573 1176 1156 930 595 345 205 164
126 123 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7480
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3164 4028 5211
Size: 416 53 83 399 24 1747 864 1183
Cuts at: 5211 6169 442
Size: 958 1753
Fragments arranged by size:
1753 1747 1183 958 864 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2517 1056
Size: 1461 6019
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4174 4174
Size: 7480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3081 3336 3521 6140 1239
Size: 1842 255 185 2619 2579
Fragments arranged by size:
2619 2579 1842 255 185
Bpu10I CC'TnA_GC
Cuts at: 2330 4014 4277 2330
Size: 1684 263 5533
Fragments arranged by size:
5533 1684 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2524 3215 5605 6470 6768 2195
Size: 329 691 2390 865 298 2907
Fragments arranged by size:
2907 2390 865 691 329 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6143 882
Size: 617 4644 2219
Fragments arranged by size:
4644 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2851 5271 493
Size: 711 1647 2420 2702
Fragments arranged by size:
2702 2420 1647 711
BsaBI GATnn'nnATC
Cuts at: 2345 7474 2345
Size: 5129 2351
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 4221 5287 5669
Size: 53 83 186 936 513 2175 1066 382
Cuts at: 5669 6304 275
Size: 635 1451
Fragments arranged by size:
2175 1451 1066 936 635 513 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2171 5913 6311 6585 6732 7143 2171
Size: 3742 398 274 147 411 2508
Fragments arranged by size:
3742 2508 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 2855 2885 4165
Size: 30 165 30 1134 30 288 30 1280
Cuts at: 4165 4195 4355 4385 1178
Size: 30 160 30 4273
Fragments arranged by size:
4273 1280 1134 288 165 160 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2515 2728 3097 1186
Size: 1329 213 369 5569
Fragments arranged by size:
5569 1329 369 213
BseYI C'CCAG_C
Cuts at: 2811 2879 3714 4039 4131 4243 4283 4291 6683
Size: 68 835 325 92 112 40 8 2392
Cuts at: 6683 7179 2811
Size: 496 3112
Fragments arranged by size:
3112 2392 835 496 325 112 92 68
40 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3266 1163
Size: 573 1530 5377
Fragments arranged by size:
5377 1530 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2257 3048 4251 4930 5691 6719
Size: 275 186 256 791 1203 679 761 1028
Cuts at: 6719 1540
Size: 2301
Fragments arranged by size:
2301 1203 1028 791 761 679 275 256
186
BsiHKAI G_wGCw'C
Cuts at: 3435 3713 4365 4388 5541 5626 6697 3435
Size: 278 652 23 1153 85 1071 4218
Fragments arranged by size:
4218 1153 1071 652 278 85 23
BsmI GAATG_Cn'
Cuts at: 2149 2764 4612 4991 5084 2149
Size: 615 1848 379 93 4545
Fragments arranged by size:
4545 1848 615 379 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2391 2581 3593 3682 2391
Size: 190 1012 89 6189
Fragments arranged by size:
6189 1012 190 89
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2336 2385 2472 2510 2598 2721
Size: 399 460 86 49 87 38 88 123
Cuts at: 2721 2828 3066 3271 3435 3519 3713 4059 4209
Size: 107 238 205 164 84 194 346 150
Cuts at: 4209 4283 4365 4388 4574 4799 5541 5626 6697
Size: 74 82 23 186 225 742 85 1071
Cuts at: 6697 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 460 399 346 238 225
205 194 186 164 150 123 107 88
87 86 85 84 82 74 49 38
23
BspEI T'CCGG_A
Cuts at: 2171 7143 2171
Size: 4972 2508
BspHI T'CATG_A
Cuts at: 5369 7099 5369
Size: 1730 5750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4678 4835 844
Size: 3834 157 3489
Fragments arranged by size:
3834 3489 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 2736 3400 4350 5367 1394
Size: 429 197 716 664 950 1017 3507
Fragments arranged by size:
3507 1017 950 716 664 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 5975 6149 65
Size: 1183 4727 174 1396
Fragments arranged by size:
4727 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2995 3020 4306 4367 6124 6311 1700
Size: 110 1185 25 1286 61 1757 187 2869
Fragments arranged by size:
2869 1757 1286 1185 187 110 61 25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7480
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7480
BssSI C'ACGA_G
Cuts at: 2057 3728 4166 4612 5540 6552 2057
Size: 1671 438 446 928 1012 2985
Fragments arranged by size:
2985 1671 1012 928 446 438
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7480
BstBI TT'CG_AA
Cuts at: 6284 6284
Size: 7480
BstEII G'GTnAC_C
Cuts at: 1184 4678 1184
Size: 3494 3986
BstXI CCAn_nnnn'nTGG
Cuts at: 2063 3112 3139 3198 3499 4291 2063
Size: 1049 27 59 301 792 5252
Fragments arranged by size:
5252 1049 792 301 59 27
BstYI r'GATC_y
Cuts at: 1972 2955 3375 3402 3555 3927 4810 5177 5562
Size: 983 420 27 153 372 883 367 385
Cuts at: 5562 5579 7031 7117 7129 7475 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 983 883 420 385 372 367
346 153 86 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2866 2937 3048 3340 3738 3882 4059
Size: 552 1801 71 111 292 398 144 177
Cuts at: 4059 4099 4176 513
Size: 40 77 3817
Fragments arranged by size:
3817 1801 552 398 292 177 144 111
77 71 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2727 487
Size: 683 56 1501 5240
Fragments arranged by size:
5240 1501 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 4996 5790 5810 2382
Size: 2614 794 20 4052
Fragments arranged by size:
4052 2614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7445
DraI TTT'AAA
Cuts at: 4784 4784
Size: 7480
DraIII CAC_nnn'GTG
Cuts at: 3328 3328
Size: 7480
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6487 809
Size: 507 5171 1802
Fragments arranged by size:
5171 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2254 2304 2430
Size: 54 1425 50 275 18 424 50 126
Cuts at: 2430 2439 3045 3200 3781 4146 4170 4248 4317
Size: 9 606 155 581 365 24 78 69
Cuts at: 4317 4927 5816 7445 8
Size: 610 889 1629 43
Fragments arranged by size:
1629 1425 889 610 606 581 424 365
275 155 126 78 69 54 50 50
43 24 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2254 3045 4248 4927 1537
Size: 275 442 791 1203 679 4090
Fragments arranged by size:
4090 1203 791 679 442 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2130 3989 5410 1353
Size: 755 22 1859 1421 3423
Fragments arranged by size:
3423 1859 1421 755 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3108 6067 6441 6587 387
Size: 1064 138 1519 2959 374 146 1280
Fragments arranged by size:
2959 1519 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4386 4386
Size: 7480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3081 3336 3356 3521 5543 6140 6927
Size: 393 1449 255 20 165 2022 597 787
Cuts at: 6927 7195 1239
Size: 268 1524
Fragments arranged by size:
2022 1524 1449 787 597 393 268 255
165 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2684
Size: 7480
EcoO109I rG'GnC_Cy
Cuts at: 2319 2381 2382 3062 3063 4757 2319
Size: 62 1 680 1 1694 5042
Fragments arranged by size:
5042 1694 680 62 1 1
EcoRI G'AATT_C
Cuts at: 4789 5167 4789
Size: 378 7102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2102 2102
Size: 7480
FseI GG_CCGG'CC
Cuts at: 3024 3024
Size: 7480
FspI TGC'GCA
Cuts at: 5986 5986
Size: 7480
HaeII r_GCGC'y
Cuts at: 1678 2818 6627 1678
Size: 1140 3809 2531
Fragments arranged by size:
3809 2531 1140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2855 2887 3285
Size: 32 135 32 965 32 286 32 398
Cuts at: 3285 3317 3360 3392 3636 3668 4629 4661 6121
Size: 32 43 32 244 32 961 32 1460
Cuts at: 6121 6153 6195 6227 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1460 965 961 398 286 244 135
43 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4824 5070 669
Size: 988 281 2886 246 3079
Fragments arranged by size:
3079 2886 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3563 748
Size: 2815 4665
HpaI GTT'AAC
Cuts at: 5070 5070
Size: 7480
KpnI G_GTAC'C
Cuts at: 2887 4805 2887
Size: 1918 5562
MfeI C'AATT_G
Cuts at: 5079 5079
Size: 7480
MluI A'CGCG_T
Cuts at: 7356 7356
Size: 7480
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3335 4433 6594 6778 1305
Size: 2030 1098 2161 184 2007
Fragments arranged by size:
2161 2030 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4172 10
Size: 54 1425 343 2340 3318
Fragments arranged by size:
3318 2340 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3170 3196 3626 5438 5797 5956 518
Size: 648 2004 26 430 1812 359 159 2042
Fragments arranged by size:
2042 2004 1812 648 430 359 159 26
MspA1I CmG'CkG
Cuts at: 1606 2520 2694 3139 3205 3392 3765 4247 4264
Size: 914 174 445 66 187 373 482 17
Cuts at: 4264 4327 4462 4896 5573 6721 6966 1606
Size: 63 135 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 677 482 445 434 373
245 187 174 135 66 63 17
NaeI GCC'GGC
Cuts at: 3022 4308 4369 3022
Size: 1286 61 6133
Fragments arranged by size:
6133 1286 61
NcoI C'CATG_G
Cuts at: 513 1065 3340 3738 4099 4176 513
Size: 552 2275 398 361 77 3817
Fragments arranged by size:
3817 2275 552 398 361 77
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7480
NgoMIV G'CCGG_C
Cuts at: 3020 4306 4367 3020
Size: 1286 61 6133
Fragments arranged by size:
6133 1286 61
NheI G'CTAG_C
Cuts at: 1052 2513 1052
Size: 1461 6019
NotI GC'GGCC_GC
Cuts at: 4927 4927
Size: 7480
NspI r_CATG'y
Cuts at: 1132 1229 3916 4838 6383 1132
Size: 97 2687 922 1545 2229
Fragments arranged by size:
2687 2229 1545 922 97
PasI CC'CwG_GG
Cuts at: 1348 4375 1348
Size: 3027 4453
PciI A'CATG_T
Cuts at: 6379 6379
Size: 7480
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2970 3328 1108
Size: 1269 593 358 5260
Fragments arranged by size:
5260 1269 593 358
PfoI T'CCnGG_A
Cuts at: 2196 2819 3498 2196
Size: 623 679 6178
Fragments arranged by size:
6178 679 623
PmeI GTTT'AAAC
Cuts at: 4784 4784
Size: 7480
PmlI CAC'GTG
Cuts at: 2851 2851
Size: 7480
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4561 4593 5549 5581 7062 7094 4561
Size: 32 956 32 1481 32 4947
Fragments arranged by size:
4947 1481 956 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4757 4757
Size: 7480
PshAI GACnn'nnGTC
Cuts at: 1613 2607 1613
Size: 994 6486
PsiI TTA'TAA
Cuts at: 5050 5050
Size: 7480
PspOMI G'GGCC_C
Cuts at: 2381 2594 3062 3267 2381
Size: 213 468 205 6594
Fragments arranged by size:
6594 468 213 205
PstI C_TGCA'G
Cuts at: 830 3164 4091 4832 830
Size: 2334 927 741 3478
Fragments arranged by size:
3478 2334 927 741
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7480
PvuII CAG'CTG
Cuts at: 2694 3392 3765 4462 2694
Size: 698 373 697 5712
Fragments arranged by size:
5712 698 697 373
SacI G_AGCT'C
Cuts at: 4388 4388
Size: 7480
SalI G'TCGA_C
Cuts at: 1936 4822 1936
Size: 2886 4594
SapI GCTCTTCn'nnn_
Cuts at: 2130 2130
Size: 7480
SbfI CC_TGCA'GG
Cuts at: 4832 4832
Size: 7480
ScaI AGT'ACT
Cuts at: 5728 7440 5728
Size: 1712 5768
SexAI A'CCwGG_T
Cuts at: 3426 3426
Size: 7480
SfcI C'TryA_G
Cuts at: 826 1045 3160 4087 4828 5963 6644 6835 826
Size: 219 2115 927 741 1135 681 191 1471
Fragments arranged by size:
2115 1471 1135 927 741 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2438 4316 2438
Size: 1878 5602
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7480
SgrAI Cr'CCGG_yG
Cuts at: 1700 2995 1700
Size: 1295 6185
SmaI CCC'GGG
Cuts at: 1872 4575 4807 1872
Size: 2703 232 4545
Fragments arranged by size:
4545 2703 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 2503 3194 3407 5584 6485
Size: 197 926 267 293 691 213 2177 901
Cuts at: 6485 6747 820
Size: 262 1553
Fragments arranged by size:
2177 1553 926 901 691 293 267 262
213 197
SnaBI TAC'GTA
Cuts at: 493 5271 493
Size: 4778 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7480
SphI G_CATG'C
Cuts at: 1132 4838 1132
Size: 3706 3774
SspI AAT'ATT
Cuts at: 5 52 5404 5
Size: 47 5352 2081
Fragments arranged by size:
5352 2081 47
StuI AGG'CCT
Cuts at: 1517 2780 4107 4424 1517
Size: 1263 1327 317 4573
Fragments arranged by size:
4573 1327 1263 317
StyI C'CwwG_G
Cuts at: 513 1065 2781 3015 3340 3510 3738 4099 4176
Size: 552 1716 234 325 170 228 361 77
Cuts at: 4176 4919 513
Size: 743 3074
Fragments arranged by size:
3074 1716 743 552 361 325 234 228
170 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4047 4112 4462 5507 5524 5677 5862
Size: 1255 2467 65 350 1045 17 153 185
Cuts at: 5862 6307 325
Size: 445 1498
Fragments arranged by size:
2467 1498 1255 1045 445 350 185 153
65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4500 5726 7438
Size: 274 80 33 51 1443 2523 1226 1712
Cuts at: 7438 96
Size: 138
Fragments arranged by size:
2523 1712 1443 1226 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4165 4197 4355 4387 7440 7472 4165
Size: 32 158 32 3053 32 4173
Fragments arranged by size:
4173 3053 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7480
XbaI T'CTAG_A
Cuts at: 4816 4816
Size: 7480
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7480
XmaI C'CCGG_G
Cuts at: 1870 4573 4805 1870
Size: 2703 232 4545
Fragments arranged by size:
4545 2703 232
XmnI GAAnn'nnTTC
Cuts at: 5609 5609
Size: 7480
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5288 276
Size: 53 83 186 936 3754 2468
Fragments arranged by size:
3754 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FspAI KasI NarI NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI
XcmI