Restriction map for
pFN21AB0452
(Circular) (Six-base) MAPSORT of: pFN21AB0452.seq Check: 3987 from: 1 to: 7462
pFN21AB0452 7462 bp
With 182 enzymes: SgfI *
October 12, 2010 15:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5272 278
Size: 53 83 186 936 3736 2468
Fragments arranged by size:
3736 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 3026 4805 1937
Size: 97 992 1779 4594
Fragments arranged by size:
4594 1779 992 97
Acc65I G'GTAC_C
Cuts at: 3510 4783 3510
Size: 1273 6189
AclI AA'CG_TT
Cuts at: 1563 5589 5962 1563
Size: 4026 373 3063
Fragments arranged by size:
4026 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2928 3786 3917 4426 5525 6909 7177
Size: 932 364 858 131 509 1099 1384 268
Cuts at: 7177 1632
Size: 1917
Fragments arranged by size:
1917 1384 1099 932 858 509 364 268
131
AfeI AGC'GCT
Cuts at: 2989 2989
Size: 7462
AflII C'TTAA_G
Cuts at: 820 1017 2518 820
Size: 197 1501 5764
Fragments arranged by size:
5764 1501 197
AflIII A'CryG_T
Cuts at: 1203 3728 4144 4189 6361 7338 1203
Size: 2525 416 45 2172 977 1327
Fragments arranged by size:
2525 2172 1327 977 416 45
AgeI A'CCGG_T
Cuts at: 6293 6293
Size: 7462
AhdI GACnn_n'nnGTC
Cuts at: 6191 6191
Size: 7462
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2447 4626 1184
Size: 1263 2179 4020
Fragments arranged by size:
4020 2179 1263
AlwNI CAG_nnn'CTG
Cuts at: 2080 2402 2443 3192 3469 3979 5205 6777 2080
Size: 322 41 749 277 510 1226 1572 2765
Fragments arranged by size:
2765 1572 1226 749 510 322 277 41
ApaI G_GGCC'C
Cuts at: 2120 2504 3578 2120
Size: 384 1074 6004
Fragments arranged by size:
6004 1074 384
ApaLI G'TGCA_C
Cuts at: 5519 6675 5519
Size: 1156 6306
ApoI r'AATT_y
Cuts at: 1493 3818 4412 4478 4771 4997 5149 7258 1493
Size: 2325 594 66 293 226 152 2109 1697
Fragments arranged by size:
2325 2109 1697 594 293 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 6016 160
Size: 872 4984 1606
Fragments arranged by size:
4984 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7462
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2378 2641 4787 1870
Size: 73 170 265 263 2146 4545
Fragments arranged by size:
4545 2146 265 263 170 73
AvrII C'CTAG_G
Cuts at: 2971 2971
Size: 7462
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3764 3797 3764
Size: 33 7429
BamHI G'GATC_C
Cuts at: 4792 5159 4792
Size: 367 7095
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2156 3510 4368 4783
Size: 325 217 627 337 32 1354 858 415
Cuts at: 4783 6238 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1354 858 627 415 337 325
217 32
BanII G_rGCy'C
Cuts at: 1391 2120 2504 2650 3005 3578 3724 3889 4236
Size: 729 384 146 355 573 146 165 347
Cuts at: 4236 4781 1391
Size: 545 4072
Fragments arranged by size:
4072 729 573 545 384 355 347 165
146 146
BbeI G_GCGC'C
Cuts at: 2160 2160
Size: 7462
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3680 3739 3842 928
Size: 971 1781 59 103 4548
Fragments arranged by size:
4548 1781 971 103 59
BbvCI CC'TCA_GC
Cuts at: 3280 3280
Size: 7462
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3050 3084 4076 4110 4603 4637 5653
Size: 34 1113 34 992 34 493 34 1016
Cuts at: 5653 5687 1903
Size: 34 3678
Fragments arranged by size:
3678 1113 1016 992 493 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2614 5354 6564 2614
Size: 2740 1210 3512
Fragments arranged by size:
3512 2740 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2313 2414 2645
Size: 107 122 71 631 605 641 101 231
Cuts at: 2645 2858 3197 3243 6073 136
Size: 213 339 46 2830 1525
Fragments arranged by size:
2830 1525 641 631 605 339 231 213
122 107 101 71 46
BglII A'GATC_T
Cuts at: 7457 7457
Size: 7462
BlpI GC'TnA_GC
Cuts at: 4879 4879
Size: 7462
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2504 2684 2854 3540 3578 4716 5523
Size: 330 384 180 170 686 38 1138 807
Cuts at: 5523 6679 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1138 807 686 384 330 180
170 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3540 4133 5193
Size: 416 53 83 399 24 2123 593 1060
Cuts at: 5193 6151 442
Size: 958 1753
Fragments arranged by size:
2123 1753 1060 958 593 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 4717 2485
Size: 2232 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2424 3185 4264 6122 1239
Size: 1148 37 761 1079 1858 2579
Fragments arranged by size:
2579 1858 1148 1079 761 37
Bpu10I CC'TnA_GC
Cuts at: 2552 3280 2552
Size: 728 6734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4362 4546 5587 6452 6750 4362
Size: 184 1041 865 298 5074
Fragments arranged by size:
5074 1041 865 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6125 882
Size: 617 4626 2219
Fragments arranged by size:
4626 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4147 4192 5253 493
Size: 711 2943 45 1061 2702
Fragments arranged by size:
2943 2702 1061 711 45
BsaBI GATnn'nnATC
Cuts at: 7456 7456
Size: 7462
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 5269 5651 6286
Size: 53 83 186 936 624 3112 382 635
Cuts at: 6286 275
Size: 1451
Fragments arranged by size:
3112 1451 936 635 624 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2455 3047 3596 4678 5895 6293 6567 6714 7125
Size: 592 549 1082 1217 398 274 147 411
Cuts at: 7125 2455
Size: 2792
Fragments arranged by size:
2792 1217 1082 592 549 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2397 2427 1178
Size: 30 165 30 994 30 6213
Fragments arranged by size:
6213 994 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2079 3977 1186
Size: 893 1898 4671
Fragments arranged by size:
4671 1898 893
BseYI C'CCAG_C
Cuts at: 2023 2497 6665 7161 2023
Size: 474 4168 496 2324
Fragments arranged by size:
4168 2324 496 474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 3273 4622 1163
Size: 573 297 1240 1349 4003
Fragments arranged by size:
4003 1349 1240 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 4522 4912 5673 6701 1540
Size: 275 186 44 2477 390 761 1028 2301
Fragments arranged by size:
2477 2301 1028 761 390 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 2906 3005 3055 3724 5523 5608 6679 2906
Size: 99 50 669 1799 85 1071 3689
Fragments arranged by size:
3689 1799 1071 669 99 85 50
BsmI GAATG_Cn'
Cuts at: 2870 4973 5066 2870
Size: 2103 93 5266
Fragments arranged by size:
5266 2103 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2504 2650 2684 2854 2906 3005
Size: 399 330 384 146 34 170 52 99
Cuts at: 3005 3055 3540 3578 3724 3889 4236 4716 4781
Size: 50 485 38 146 165 347 480 65
Cuts at: 4781 5523 5608 6679 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 480 399 384 347
330 170 165 146 146 99 85 65
52 50 38 34
BspEI T'CCGG_A
Cuts at: 2455 3047 3596 4678 7125 2455
Size: 592 549 1082 2447 2792
Fragments arranged by size:
2792 2447 1082 592 549
BspHI T'CATG_A
Cuts at: 5351 7081 5351
Size: 1730 5732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3686 4650 4817 844
Size: 2842 964 167 3489
Fragments arranged by size:
3489 2842 964 167
BsrBI CCG'CTC
Cuts at: 1394 1823 5349 1394
Size: 429 3526 3507
Fragments arranged by size:
3526 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4527 5957 6131 65
Size: 1183 3279 1430 174 1396
Fragments arranged by size:
3279 1430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6106 6293 1700
Size: 110 4296 187 2869
Fragments arranged by size:
4296 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4141 4344 96
Size: 4045 203 3214
Fragments arranged by size:
4045 3214 203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7462
BssSI C'ACGA_G
Cuts at: 5522 6534 5522
Size: 1012 6450
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 794
Size: 1649 5813
BstBI TT'CG_AA
Cuts at: 4495 6266 4495
Size: 1771 5691
BstEII G'GTnAC_C
Cuts at: 1184 3273 1184
Size: 2089 5373
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 3338 4591 2743
Size: 595 1253 5614
Fragments arranged by size:
5614 1253 595
BstYI r'GATC_y
Cuts at: 1972 4719 4792 5159 5544 5561 7013 7099 7111
Size: 2747 73 367 385 17 1452 86 12
Cuts at: 7111 7457 1972
Size: 346 1977
Fragments arranged by size:
2747 1977 1452 385 367 346 86 73
17 12
Bsu36I CC'TnA_GG
Cuts at: 3462 3462
Size: 7462
BtgI C'CryG_G
Cuts at: 513 1065 2004 2412 2736 4248 513
Size: 552 939 408 324 1512 3727
Fragments arranged by size:
3727 1512 939 552 408 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6723
Fragments arranged by size:
6723 683 56
BtsI GCAGTG_nn'
Cuts at: 2730 3218 4430 4978 5772 5792 2730
Size: 488 1212 548 794 20 4400
Fragments arranged by size:
4400 1212 794 548 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7462
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7427
DraI TTT'AAA
Cuts at: 4766 4766
Size: 7462
DraIII CAC_nnn'GTG
Cuts at: 4663 4663
Size: 7462
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6469 809
Size: 507 5153 1802
Fragments arranged by size:
5153 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2415 2859
Size: 54 1425 50 275 18 212 373 444
Cuts at: 2859 3111 4909 5798 7427 8
Size: 252 1798 889 1629 43
Fragments arranged by size:
1798 1629 1425 889 444 373 275 252
212 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2042 4909 1537
Size: 275 230 2867 4090
Fragments arranged by size:
4090 2867 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 3317 4445 5392 1353
Size: 1964 1128 947 3423
Fragments arranged by size:
3423 1964 1128 947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3120 6049 6423 6569 387
Size: 1064 138 1531 2929 374 146 1280
Fragments arranged by size:
2929 1531 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3003 3722 3003
Size: 719 6743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2424 2564 2928 3185 3786 3917
Size: 393 755 37 140 364 257 601 131
Cuts at: 3917 4264 4426 5525 6122 6909 7177 1239
Size: 347 162 1099 597 787 268 1524
Fragments arranged by size:
1524 1099 787 755 601 597 393 364
347 268 257 162 140 131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2555 2330
Size: 225 7237
EcoO109I rG'GnC_Cy
Cuts at: 2117 2306 2501 3226 3260 3575 2117
Size: 189 195 725 34 315 6004
Fragments arranged by size:
6004 725 315 195 189 34
EcoRI G'AATT_C
Cuts at: 4412 4771 5149 4412
Size: 359 378 6725
Fragments arranged by size:
6725 378 359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3766 3766
Size: 7462
FspI TGC'GCA
Cuts at: 5968 5968
Size: 7462
HaeII r_GCGC'y
Cuts at: 1678 2160 2991 3192 6609 1678
Size: 482 831 201 3417 2531
Fragments arranged by size:
3417 2531 831 482 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3578 3610 6103
Size: 32 135 32 804 32 1170 32 2493
Cuts at: 6103 6135 6177 6209 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2493 1170 804 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4276 4806 5052 669
Size: 988 281 2338 530 246 3079
Fragments arranged by size:
3079 2338 988 530 281 246
HindIII A'AGCT_T
Cuts at: 748 3699 748
Size: 2951 4511
HpaI GTT'AAC
Cuts at: 5052 5052
Size: 7462
KasI G'GCGC_C
Cuts at: 2156 2156
Size: 7462
KpnI G_GTAC'C
Cuts at: 3514 4787 3514
Size: 1273 6189
MfeI C'AATT_G
Cuts at: 5061 5061
Size: 7462
MluI A'CGCG_T
Cuts at: 7338 7338
Size: 7462
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2739 6576 6760 1305
Size: 1434 3837 184 2007
Fragments arranged by size:
3837 2007 1434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5640
Fragments arranged by size:
5640 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4703 5420 5779 5938 518
Size: 648 843 2694 717 359 159 2042
Fragments arranged by size:
2694 2042 843 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2443 2474 2526 2534 2622 2831 3068 3175
Size: 837 31 52 8 88 209 237 107
Cuts at: 3175 3257 3764 4878 5555 6703 6948 1606
Size: 82 507 1114 677 1148 245 2120
Fragments arranged by size:
2120 1148 1114 837 677 507 245 237
209 107 88 82 52 31 8
NarI GG'CG_CC
Cuts at: 2157 2157
Size: 7462
NcoI C'CATG_G
Cuts at: 513 1065 2004 2736 4248 513
Size: 552 939 732 1512 3727
Fragments arranged by size:
3727 1512 939 732 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7462
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7462
NotI GC'GGCC_GC
Cuts at: 4909 4909
Size: 7462
NruI TCG'CGA
Cuts at: 3084 3084
Size: 7462
NspI r_CATG'y
Cuts at: 1132 1229 2803 3616 3732 4330 4536 4820 6365
Size: 97 1574 813 116 598 206 284 1545
Cuts at: 6365 1132
Size: 2229
Fragments arranged by size:
2229 1574 1545 813 598 284 206 116
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7462
PciI A'CATG_T
Cuts at: 3728 6361 3728
Size: 2633 4829
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2402 4254 4460 1108
Size: 1294 1852 206 4110
Fragments arranged by size:
4110 1852 1294 206
PfoI T'CCnGG_A
Cuts at: 2325 3005 4032 4223 4280 4337 4590 2325
Size: 680 1027 191 57 57 253 5197
Fragments arranged by size:
5197 1027 680 253 191 57 57
PmeI GTTT'AAAC
Cuts at: 4766 4766
Size: 7462
PmlI CAC'GTG
Cuts at: 4147 4192 4147
Size: 45 7417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5531 5563 7044 7076 5531
Size: 32 1481 32 5917
Fragments arranged by size:
5917 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3226 3260 3226
Size: 34 7428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7462
PsiI TTA'TAA
Cuts at: 5032 5032
Size: 7462
PspOMI G'GGCC_C
Cuts at: 2116 2500 3574 2116
Size: 384 1074 6004
Fragments arranged by size:
6004 1074 384
PstI C_TGCA'G
Cuts at: 830 2178 2995 4814 830
Size: 1348 817 1819 3478
Fragments arranged by size:
3478 1819 1348 817
PvuI CG_AT'CG
Cuts at: 2001 4522 2001
Size: 2521 4941
PvuII CAG'CTG
Cuts at: 2443 2474 2526 3068 2443
Size: 31 52 542 6837
Fragments arranged by size:
6837 542 52 31
RsrII CG'GwC_CG
Cuts at: 3116 3116
Size: 7462
SacI G_AGCT'C
Cuts at: 3005 3724 3005
Size: 719 6743
SalI G'TCGA_C
Cuts at: 1936 4804 1936
Size: 2868 4594
SanDI GG'GwC_CC
Cuts at: 3260 3260
Size: 7462
SbfI CC_TGCA'GG
Cuts at: 4814 4814
Size: 7462
ScaI AGT'ACT
Cuts at: 4220 5710 7422 4220
Size: 1490 1712 4260
Fragments arranged by size:
4260 1712 1490
SexAI A'CCwGG_T
Cuts at: 2319 2319
Size: 7462
SfcI C'TryA_G
Cuts at: 826 1045 2174 2991 3432 3920 4810 5945 6626
Size: 219 1129 817 441 488 890 1135 681
Cuts at: 6626 6817 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1129 890 817 681 488 441
219 191
SfoI GGC'GCC
Cuts at: 2158 2158
Size: 7462
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7462
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7462
SmaI CCC'GGG
Cuts at: 1872 2115 4789 1872
Size: 243 2674 4545
Fragments arranged by size:
4545 2674 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2518 4377 4561 5566 6467 6729
Size: 197 926 575 1859 184 1005 901 262
Cuts at: 6729 820
Size: 1553
Fragments arranged by size:
1859 1553 1005 926 901 575 262 197
184
SnaBI TAC'GTA
Cuts at: 493 5253 493
Size: 4760 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7462
SphI G_CATG'C
Cuts at: 1132 4330 4536 4820 1132
Size: 3198 206 284 3774
Fragments arranged by size:
3774 3198 284 206
SspI AAT'ATT
Cuts at: 5 52 5386 5
Size: 47 5334 2081
Fragments arranged by size:
5334 2081 47
StuI AGG'CCT
Cuts at: 1517 4166 1517
Size: 2649 4813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 2706 2736 2971 4161 4248
Size: 552 939 657 45 30 235 1190 87
Cuts at: 4248 4901 513
Size: 653 3074
Fragments arranged by size:
3074 1190 939 657 653 552 235 87
45 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 2735 3696 4772 5489 5506 5659
Size: 1255 562 593 961 1076 717 17 153
Cuts at: 5659 5844 6289 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1076 961 717 593 562 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4141 4218 4344
Size: 274 80 33 51 1443 2164 77 126
Cuts at: 4344 5708 7420 96
Size: 1364 1712 138
Fragments arranged by size:
2164 1712 1443 1364 274 138 126 80
77 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 4403 4435 7422 7454 2397
Size: 32 1974 32 2987 32 2405
Fragments arranged by size:
2987 2405 1974 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3985 1316
Size: 2669 4793
XbaI T'CTAG_A
Cuts at: 4798 4798
Size: 7462
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 2317
Size: 7462
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7462
XmaI C'CCGG_G
Cuts at: 1870 2113 4787 1870
Size: 243 2674 4545
Fragments arranged by size:
4545 2674 243
XmnI GAAnn'nnTTC
Cuts at: 2081 2784 5591 2081
Size: 703 2807 3952
Fragments arranged by size:
3952 2807 703
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5270 276
Size: 53 83 186 936 3736 2468
Fragments arranged by size:
3736 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI
SacII SapI SfiI SrfI SwaI