Restriction map for
pFN21AB0450
(Circular) (Six-base) MAPSORT of: pFN21AB0450.seq Check: 6905 from: 1 to: 7294
pFN21AB0450 7294 bp
With 182 enzymes: SgfI *
March 28, 2012 12:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2178 2178
Size: 7294
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5104 278
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3776 4637 1937
Size: 1839 861 4594
Fragments arranged by size:
4594 1839 861
Acc65I G'GTAC_C
Cuts at: 4615 4615
Size: 7294
AclI AA'CG_TT
Cuts at: 1563 3298 5421 5794 1563
Size: 1735 2123 373 3063
Fragments arranged by size:
3063 2123 1735 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3607 3665 5357 6741 7009 1632
Size: 1975 58 1692 1384 268 1917
Fragments arranged by size:
1975 1917 1692 1384 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7097
AflIII A'CryG_T
Cuts at: 1203 3101 3242 6193 7170 1203
Size: 1898 141 2951 977 1327
Fragments arranged by size:
2951 1898 1327 977 141
AgeI A'CCGG_T
Cuts at: 6125 6125
Size: 7294
AhdI GACnn_n'nnGTC
Cuts at: 6023 6023
Size: 7294
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2576 1184
Size: 1392 5902
AlwNI CAG_nnn'CTG
Cuts at: 2262 5037 6609 2262
Size: 2775 1572 2947
Fragments arranged by size:
2947 2775 1572
ApaLI G'TGCA_C
Cuts at: 5351 6507 5351
Size: 1156 6138
ApoI r'AATT_y
Cuts at: 1493 2750 3180 3191 4603 4829 4981 7090 1493
Size: 1257 430 11 1412 226 152 2109 1697
Fragments arranged by size:
2109 1697 1412 1257 430 226 152 11
AseI AT'TA_AT
Cuts at: 160 1032 2501 3020 5848 160
Size: 872 1469 519 2828 1606
Fragments arranged by size:
2828 1606 1469 872 519
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
AvaI C'yCGr_G
Cuts at: 1870 1943 4619 1870
Size: 73 2676 4545
Fragments arranged by size:
4545 2676 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2380 2413 2380
Size: 33 7261
BamHI G'GATC_C
Cuts at: 4624 4991 4624
Size: 367 6927
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4615 6070 618
Size: 325 217 627 2828 1455 1842
Fragments arranged by size:
2828 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 4067 4613 1391
Size: 630 2046 546 4072
Fragments arranged by size:
4072 2046 630 546
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2297 4260 928
Size: 971 398 1963 3962
Fragments arranged by size:
3962 1963 971 398
BbvCI CC'TCA_GC
Cuts at: 3924 3924
Size: 7294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5485 5519 1903
Size: 34 3548 34 3678
Fragments arranged by size:
3678 3548 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4131 5186 6396 4131
Size: 1055 1210 5029
Fragments arranged by size:
5029 1210 1055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7294
BfrBI ATG'CAT
Cuts at: 4091 4091
Size: 7294
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2967 4399 5905
Size: 107 122 71 631 605 1295 1432 1506
Cuts at: 5905 136
Size: 1525
Fragments arranged by size:
1525 1506 1432 1295 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7289 7289
Size: 7294
BlpI GC'TnA_GC
Cuts at: 4711 4711
Size: 7294
Bme1580I G_kGCm'C
Cuts at: 1790 5355 6511 1790
Size: 3565 1156 2573
Fragments arranged by size:
3565 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7294
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2670 5025 5983
Size: 416 53 83 399 24 1253 2355 958
Cuts at: 5983 442
Size: 1753
Fragments arranged by size:
2355 1753 1253 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5954 1239
Size: 4715 2579
Bpu10I CC'TnA_GC
Cuts at: 2080 3924 2080
Size: 1844 5450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3142 4544 5419 6284 6582 3142
Size: 1402 875 865 298 3854
Fragments arranged by size:
3854 1402 875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3072 3417 5957 882
Size: 617 1573 345 2540 2219
Fragments arranged by size:
2540 2219 1573 617 345
BsaAI yAC'GTr
Cuts at: 493 1204 3243 4273 5085 493
Size: 711 2039 1030 812 2702
Fragments arranged by size:
2702 2039 1030 812 711
BsaBI GATnn'nnATC
Cuts at: 7288 7288
Size: 7294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5101 5483 6118 275
Size: 53 83 186 936 3568 382 635 1451
Fragments arranged by size:
3568 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2171 5727 6125 6399 6546 6957 2171
Size: 3556 398 274 147 411 2508
Fragments arranged by size:
3556 2508 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2584 2614 4185 4215 1178
Size: 30 165 30 1181 30 1571 30 4257
Fragments arranged by size:
4257 1571 1181 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 3785 4427 1186
Size: 843 1756 642 4053
Fragments arranged by size:
4053 1756 843 642
BseYI C'CCAG_C
Cuts at: 2304 6497 6993 2304
Size: 4193 496 2605
Fragments arranged by size:
4193 2605 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2076 3688 1163
Size: 573 340 1612 4769
Fragments arranged by size:
4769 1612 573 340
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4744 5505 6533 1540
Size: 275 186 2743 761 1028 2301
Fragments arranged by size:
2743 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 4067 5355 5440 6511 2021
Size: 2046 1288 85 1071 2804
Fragments arranged by size:
2804 2046 1288 1071 85
BsmI GAATG_Cn'
Cuts at: 2477 4091 4805 4898 2477
Size: 1614 714 93 4873
Fragments arranged by size:
4873 1614 714 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 4067 4613 5355 5440 6511 1391
Size: 399 231 2046 546 742 85 1071 2174
Fragments arranged by size:
2174 2046 1071 742 546 399 231 85
BspEI T'CCGG_A
Cuts at: 2171 6957 2171
Size: 4786 2508
BspHI T'CATG_A
Cuts at: 2896 4461 5183 6913 2896
Size: 1565 722 1730 3277
Fragments arranged by size:
3277 1730 1565 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 3817 4578 4649 844
Size: 1334 1639 761 71 3489
Fragments arranged by size:
3489 1639 1334 761 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2854 5181 1394
Size: 429 1031 2327 3507
Fragments arranged by size:
3507 2327 1031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5789 5963 65
Size: 1183 4541 174 1396
Fragments arranged by size:
4541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2614 4398 5938 6125 1700
Size: 110 804 1784 1540 187 2869
Fragments arranged by size:
2869 1784 1540 804 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7294
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7294
BssSI C'ACGA_G
Cuts at: 2849 5354 6366 2849
Size: 2505 1012 3777
Fragments arranged by size:
3777 2505 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7294
BstBI TT'CG_AA
Cuts at: 6098 6098
Size: 7294
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7294
BstXI CCAn_nnnn'nTGG
Cuts at: 4330 4330
Size: 7294
BstYI r'GATC_y
Cuts at: 1972 2174 4624 4991 5376 5393 6845 6931 6943
Size: 202 2450 367 385 17 1452 86 12
Cuts at: 6943 7289 1972
Size: 346 1977
Fragments arranged by size:
2450 1977 1452 385 367 346 202 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2299 2514 3082 3427 3586 513
Size: 552 939 295 215 568 345 159 4221
Fragments arranged by size:
4221 939 568 552 345 295 215 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6555
Fragments arranged by size:
6555 683 56
BtsI GCAGTG_nn'
Cuts at: 2088 2359 2882 3819 4810 5604 5624 2088
Size: 271 523 937 991 794 20 3758
Fragments arranged by size:
3758 991 937 794 523 271 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7294
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7259
DraI TTT'AAA
Cuts at: 4598 4598
Size: 7294
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6301 809
Size: 507 4985 1802
Fragments arranged by size:
4985 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2655 4741
Size: 54 1425 50 275 18 549 276 2086
Cuts at: 4741 5630 7259 8
Size: 889 1629 43
Fragments arranged by size:
2086 1629 1425 889 549 276 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4741 1537
Size: 275 2929 4090
Fragments arranged by size:
4090 2929 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3777 4072 5224 1353
Size: 2424 295 1152 3423
Fragments arranged by size:
3423 2424 1152 295
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5881 6255 6401 387
Size: 1064 138 4292 374 146 1280
Fragments arranged by size:
4292 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2019 4065 2019
Size: 2046 5248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3607 3665 5357 5954 6741 7009 1239
Size: 393 1975 58 1692 597 787 268 1524
Fragments arranged by size:
1975 1692 1524 787 597 393 268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2608 2608
Size: 7294
EcoO109I rG'GnC_Cy
Cuts at: 2129 3582 2129
Size: 1453 5841
EcoRI G'AATT_C
Cuts at: 3180 4603 4981 3180
Size: 1423 378 5493
Fragments arranged by size:
5493 1423 378
EcoRV GAT'ATC
Cuts at: 3334 3838 3334
Size: 504 6790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3393 3292
Size: 101 7193
FspI TGC'GCA
Cuts at: 3611 5800 3611
Size: 2189 5105
HaeII r_GCGC'y
Cuts at: 1678 6441 1678
Size: 4763 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2968 2971 3000
Size: 32 135 32 486 32 878 3 29
Cuts at: 3000 3003 3780 3812 3822 3854 3979 4011 4424
Size: 3 777 32 10 32 125 32 413
Cuts at: 4424 4456 5935 5967 6009 6041 1373
Size: 32 1479 32 42 32 2626
Fragments arranged by size:
2626 1479 878 777 486 413 135 125
42 32 32 32 32 32 32 32
32 32 29 10 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2777 3061 3448 4198 4638 4884
Size: 988 281 839 284 387 750 440 246
Cuts at: 4884 669
Size: 3079
Fragments arranged by size:
3079 988 839 750 440 387 284 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7294
HpaI GTT'AAC
Cuts at: 4198 4884 4198
Size: 686 6608
KpnI G_GTAC'C
Cuts at: 4619 4619
Size: 7294
MfeI C'AATT_G
Cuts at: 4893 4893
Size: 7294
MluI A'CGCG_T
Cuts at: 7170 7170
Size: 7294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 3055 3720 4322 6408 6592 1305
Size: 739 1011 665 602 2086 184 2007
Fragments arranged by size:
2086 2007 1011 739 665 602 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 2657 10
Size: 54 1425 343 549 276 4647
Fragments arranged by size:
4647 1425 549 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3054 4466 4578 5252 5611 5770
Size: 648 1789 99 1412 112 674 359 159
Cuts at: 5770 518
Size: 2042
Fragments arranged by size:
2042 1789 1412 674 648 359 159 112
99
MspA1I CmG'CkG
Cuts at: 1606 4326 4710 5387 6535 6780 1606
Size: 2720 384 677 1148 245 2120
Fragments arranged by size:
2720 2120 1148 677 384 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3082 3586 513
Size: 552 939 1078 504 4221
Fragments arranged by size:
4221 1078 939 552 504
NdeI CA'TA_TG
Cuts at: 387 4579 387
Size: 4192 3102
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7294
NotI GC'GGCC_GC
Cuts at: 4741 4741
Size: 7294
NsiI A_TGCA'T
Cuts at: 4093 4093
Size: 7294
NspI r_CATG'y
Cuts at: 1132 1229 3105 3365 3457 4652 6197 1132
Size: 97 1876 260 92 1195 1545 2229
Fragments arranged by size:
2229 1876 1545 1195 260 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7294
PciI A'CATG_T
Cuts at: 3101 6193 3101
Size: 3092 4202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3088 3343 1108
Size: 1980 255 5059
Fragments arranged by size:
5059 1980 255
PmeI GTTT'AAAC
Cuts at: 4598 4598
Size: 7294
PmlI CAC'GTG
Cuts at: 3243 3243
Size: 7294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5363 5395 6876 6908 5363
Size: 32 1481 32 5749
Fragments arranged by size:
5749 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2129 3582 2129
Size: 1453 5841
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7294
PsiI TTA'TAA
Cuts at: 3390 4864 3390
Size: 1474 5820
PstI C_TGCA'G
Cuts at: 830 2482 3814 4646 830
Size: 1652 1332 832 3478
Fragments arranged by size:
3478 1652 1332 832
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7294
PvuII CAG'CTG
Cuts at: 4326 4326
Size: 7294
SacI G_AGCT'C
Cuts at: 2021 4067 2021
Size: 2046 5248
SalI G'TCGA_C
Cuts at: 1936 4636 1936
Size: 2700 4594
SanDI GG'GwC_CC
Cuts at: 3582 3582
Size: 7294
SapI GCTCTTCn'nnn_
Cuts at: 4072 4072
Size: 7294
SbfI CC_TGCA'GG
Cuts at: 2482 4646 2482
Size: 2164 5130
ScaI AGT'ACT
Cuts at: 5542 7254 5542
Size: 1712 5582
SfcI C'TryA_G
Cuts at: 826 1045 2136 2478 3777 3810 4309 4642 5777
Size: 219 1091 342 1299 33 499 333 1135
Cuts at: 5777 6458 6649 826
Size: 681 191 1471
Fragments arranged by size:
1471 1299 1135 1091 681 499 342 333
219 191 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 2967 2967
Size: 7294
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7294
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7294
SmaI CCC'GGG
Cuts at: 1872 4621 1872
Size: 2749 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3121 4559 5398 6299 6561 820
Size: 197 926 1178 1438 839 901 262 1553
Fragments arranged by size:
1553 1438 1178 926 901 839 262 197
SnaBI TAC'GTA
Cuts at: 493 5085 493
Size: 4592 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7294
SphI G_CATG'C
Cuts at: 1132 3457 4652 1132
Size: 2325 1195 3774
Fragments arranged by size:
3774 2325 1195
SspI AAT'ATT
Cuts at: 5 52 2569 2862 3879 5218 5
Size: 47 2517 293 1017 1339 2081
Fragments arranged by size:
2517 2081 1339 1017 293 47
StuI AGG'CCT
Cuts at: 1517 2905 1517
Size: 1388 5906
StyI C'CwwG_G
Cuts at: 513 1065 2004 3082 3586 4733 513
Size: 552 939 1078 504 1147 3074
Fragments arranged by size:
3074 1147 1078 939 552 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2899 5321 5338 5491 5676 6121 325
Size: 1255 1319 2422 17 153 185 445 1498
Fragments arranged by size:
2422 1498 1319 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 3492 5540
Size: 274 80 33 51 1443 335 1180 2048
Cuts at: 5540 7252 96
Size: 1712 138
Fragments arranged by size:
2048 1712 1443 1180 335 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2584 2616 4185 4217 7254 7286 2584
Size: 32 1569 32 3037 32 2592
Fragments arranged by size:
3037 2592 1569 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7294
XbaI T'CTAG_A
Cuts at: 4630 4630
Size: 7294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2407 2407
Size: 7294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7294
XmaI C'CCGG_G
Cuts at: 1870 4619 1870
Size: 2749 4545
XmnI GAAnn'nnTTC
Cuts at: 4148 5423 4148
Size: 1275 6019
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5102 276
Size: 53 83 186 936 3568 2468
Fragments arranged by size:
3568 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SexAI SfoI SrfI SwaI