Restriction map for
pFN21AB0449
(Circular) (Six-base) MAPSORT of: pFN21AB0449.seq Check: 204 from: 1 to: 6127
pFN21AB0449 6127 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2283 3937 278
Size: 53 83 186 936 747 1654 2468
Fragments arranged by size:
2468 1654 936 747 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 2615 2702 2822 2861 3356 4190 5574
Size: 974 9 87 120 39 495 834 1384
Cuts at: 5574 5842 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 974 834 495 268 120 87
39 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 2654 1166
Size: 1488 4639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2804 1184
Size: 1620 4507
AlwNI CAG_nnn'CTG
Cuts at: 2692 2815 3257 3870 5442 2692
Size: 123 442 613 1572 3377
Fragments arranged by size:
3377 1572 613 442 123
ApaLI G'TGCA_C
Cuts at: 3299 4184 5340 3299
Size: 885 1156 4086
Fragments arranged by size:
4086 1156 885
ApoI r'AATT_y
Cuts at: 1493 2090 2327 3436 3662 3814 5923 1493
Size: 597 237 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 597 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3028 3074 3452 1870
Size: 73 1085 46 378 4545
Fragments arranged by size:
4545 1085 378 73 46
BamHI G'GATC_C
Cuts at: 2945 3457 3824 2945
Size: 512 367 5248
Fragments arranged by size:
5248 512 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 3448 4903 618
Size: 325 217 627 675 986 1455 1842
Fragments arranged by size:
1842 1455 986 675 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2357 3035 3446 1391
Size: 966 678 411 4072
Fragments arranged by size:
4072 966 678 411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2470 3318 928
Size: 971 571 848 3737
Fragments arranged by size:
3737 971 848 571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3181 4019 5229 3181
Size: 838 1210 4079
Fragments arranged by size:
4079 1210 838
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2359 6122 2359
Size: 3763 2364
BlpI GC'TnA_GC
Cuts at: 2375 3544 2375
Size: 1169 4958
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2465 2883 3303 4188 5344 1790
Size: 268 407 418 420 885 1156 2573
Fragments arranged by size:
2573 1156 885 420 418 407 268
BmgBI CAC'GTC
Cuts at: 1318 3096 1318
Size: 1778 4349
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2462 3858 4816
Size: 416 53 83 399 24 1045 1396 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1753 1396 1045 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2313 2313
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 3335 4787 1239
Size: 1316 780 1452 2579
Fragments arranged by size:
2579 1452 1316 780
Bpu10I CC'TnA_GC
Cuts at: 3209 3209
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2154 3288 4790 882
Size: 617 561 94 1134 1502 2219
Fragments arranged by size:
2219 1502 1134 617 561 94
BsaAI yAC'GTr
Cuts at: 493 1204 2437 3918 493
Size: 711 1233 1481 2702
Fragments arranged by size:
2702 1481 1233 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 3934 4316 4951
Size: 53 83 186 936 747 1654 382 635
Cuts at: 4951 275
Size: 1451
Fragments arranged by size:
1654 1451 936 747 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3279 4560 4958 5232 5379 5790 3279
Size: 1281 398 274 147 411 3616
Fragments arranged by size:
3616 1281 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3282 3305 3312 3335 1178
Size: 30 165 30 1879 23 7 23 3970
Fragments arranged by size:
3970 1879 165 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 2558 3062 1186
Size: 1036 336 504 4251
Fragments arranged by size:
4251 1036 504 336
BseYI C'CCAG_C
Cuts at: 2941 5330 5826 2941
Size: 2389 496 3242
Fragments arranged by size:
3242 2389 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2197 2255 2542 2774 3038 1163
Size: 573 461 58 287 232 264 4252
Fragments arranged by size:
4252 573 461 287 264 232 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 3303 4188 4273 5344 2541
Size: 762 885 85 1071 3324
Fragments arranged by size:
3324 1071 885 762 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
BsmBI CGTCTCn'nnnn_
Cuts at: 3086 3086
Size: 6127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2357 2465 2541 2883 3035 3303
Size: 399 268 299 108 76 342 152 268
Cuts at: 3303 3446 4188 4273 5344 1391
Size: 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 299 268 268
152 143 108 85 76
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 2010 2712 4016 5746 2010
Size: 702 1304 1730 2391
Fragments arranged by size:
2391 1730 1304 702
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 3482 844
Size: 1979 659 3489
Fragments arranged by size:
3489 1979 659
BsrBI CCG'CTC
Cuts at: 1394 1823 2374 3163 4014 1394
Size: 429 551 789 851 3507
Fragments arranged by size:
3507 851 789 551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 4771 4958 1700
Size: 110 311 2650 187 2869
Fragments arranged by size:
2869 2650 311 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 2540 4187 5199 2540
Size: 1647 1012 3468
Fragments arranged by size:
3468 1647 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 794
Size: 2463 3664
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 2908 1184
Size: 1724 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2359 2526 2784 2945 3342 3457 3824 4209
Size: 387 167 258 161 397 115 367 385
Cuts at: 4209 4226 5678 5764 5776 6122 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 397 387 385 367 346 258
167 161 115 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2271 2304 513
Size: 552 1206 33 4336
Fragments arranged by size:
4336 1206 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2559 3315 3643 4437 4457 2559
Size: 756 328 794 20 4229
Fragments arranged by size:
4229 794 756 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2950 2985 529
Size: 35 2386 35 3671
Fragments arranged by size:
3671 2386 35 35
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2847 2862 3006
Size: 54 1425 50 275 18 1017 15 144
Cuts at: 3006 3574 4463 6092 8
Size: 568 889 1629 43
Fragments arranged by size:
1629 1425 1017 889 568 275 144 54
50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3029 3084 4057 1353
Size: 1676 55 973 3423
Fragments arranged by size:
3423 1676 973 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2140 2303 4714 5088 5234 387
Size: 1064 138 551 163 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 551 374 163 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 2606 2615 2702 2822 2861 3335
Size: 393 923 51 9 87 120 39 474
Cuts at: 3335 3356 4190 4787 5574 5842 1239
Size: 21 834 597 787 268 1524
Fragments arranged by size:
1524 923 834 787 597 474 393 268
120 87 51 39 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2321
Size: 6127
EcoO109I rG'GnC_Cy
Cuts at: 2758 2797 2925 3018 3078 3358 2758
Size: 39 128 93 60 280 5527
Fragments arranged by size:
5527 280 128 93 60 39
EcoRI G'AATT_C
Cuts at: 2090 2327 3436 3814 2090
Size: 237 1109 378 4403
Fragments arranged by size:
4403 1109 378 237
EcoRV GAT'ATC
Cuts at: 2311 2311
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2038 2263 2555 5274 1678
Size: 360 225 292 2719 2531
Fragments arranged by size:
2719 2531 360 292 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 3305 3337 4768
Size: 32 135 32 772 32 929 32 1431
Cuts at: 4768 4800 4842 4874 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1431 929 772 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3165 5241 5425 1305
Size: 1860 2076 184 2007
Fragments arranged by size:
2076 2007 1860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2864 3008 10
Size: 54 1425 343 1032 144 3129
Fragments arranged by size:
3129 1425 1032 343 144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2654 2717 2972 4085 4444 4603
Size: 648 843 645 63 255 1113 359 159
Cuts at: 4603 518
Size: 2042
Fragments arranged by size:
2042 1113 843 648 645 359 255 159
63
MspA1I CmG'CkG
Cuts at: 1606 2202 2245 2749 3543 4220 5368 5613 1606
Size: 596 43 504 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 596 504 245 43
NcoI C'CATG_G
Cuts at: 513 1065 2271 2304 513
Size: 552 1206 33 4336
Fragments arranged by size:
4336 1206 552 33
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2386 2722 3247 3485 5030 1132
Size: 97 1157 336 525 238 1545 2229
Fragments arranged by size:
2229 1545 1157 525 336 238 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 3078 3358 2797
Size: 281 280 5566
Fragments arranged by size:
5566 281 280
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2749 2749
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SanDI GG'GwC_CC
Cuts at: 3078 3078
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 2957 4375 6087 2957
Size: 1418 1712 2997
Fragments arranged by size:
2997 1712 1418
SfcI C'TryA_G
Cuts at: 826 1045 2333 2342 3133 3475 4610 5291 5482
Size: 219 1288 9 791 342 1135 681 191
Cuts at: 5482 826
Size: 1471
Fragments arranged by size:
1471 1288 1135 791 681 342 219 191
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3030 3454 1872
Size: 1158 424 4545
Fragments arranged by size:
4545 1158 424
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4231 5132 5394 820
Size: 197 926 2288 901 262 1553
Fragments arranged by size:
2288 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 2722 3485 1132
Size: 1590 763 3774
Fragments arranged by size:
3774 1590 763
SspI AAT'ATT
Cuts at: 5 52 2608 4051 5
Size: 47 2556 1443 2081
Fragments arranged by size:
2556 2081 1443 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2082 2271 2304 3566 513
Size: 552 1017 189 33 1262 3074
Fragments arranged by size:
3074 1262 1017 552 189 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2631 4154 4171 4324 4509 4954
Size: 1255 555 496 1523 17 153 185 445
Cuts at: 4954 325
Size: 1498
Fragments arranged by size:
1523 1498 1255 555 496 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2955 4373 6085
Size: 274 80 33 51 1443 978 1418 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
1712 1443 1418 978 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3282 3314 6087 6119 3282
Size: 32 2773 32 3290
Fragments arranged by size:
3290 2773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3028 3452 1870
Size: 1158 424 4545
Fragments arranged by size:
4545 1158 424
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2281 3935 276
Size: 53 83 186 936 747 1654 2468
Fragments arranged by size:
2468 1654 936 747 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI