Restriction map for
pFN21AB0448
(Circular) (Six-base) MAPSORT of: pFN21AB0448.seq Check: 4855 from: 1 to: 7438
pFN21AB0448 7438 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4781 1937
Size: 2844 4594
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3100 3632 4436 4679 5501 6885 7153
Size: 653 815 532 804 243 822 1384 268
Cuts at: 7153 1632
Size: 1917
Fragments arranged by size:
1917 1384 822 815 804 653 532 268
243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 6337 7314 1203
Size: 5134 977 1327
Fragments arranged by size:
5134 1327 977
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 4544 6167 4544
Size: 1623 5815
AleI CACnn'nnGTG
Cuts at: 1166 4403 1166
Size: 3237 4201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4155 1184
Size: 2971 4467
AlwNI CAG_nnn'CTG
Cuts at: 2803 3217 3596 4343 5181 6753 2803
Size: 414 379 747 838 1572 3488
Fragments arranged by size:
3488 1572 838 747 414 379
ApaI G_GGCC'C
Cuts at: 4202 4268 4304 4202
Size: 66 36 7336
Fragments arranged by size:
7336 66 36
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2180 2405 2750 2984 4348 4747 4973 5125
Size: 687 225 345 234 1364 399 226 152
Cuts at: 5125 7234 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1364 687 399 345 234 226
225 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 4763 1870
Size: 73 2820 4545
Fragments arranged by size:
4545 2820 73
BamHI G'GATC_C
Cuts at: 3261 4768 5135 3261
Size: 1507 367 5564
Fragments arranged by size:
5564 1507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3415 4759 6214 618
Size: 325 217 627 1628 1344 1455 1842
Fragments arranged by size:
1842 1628 1455 1344 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4202 4268 4304 4757 1391
Size: 2811 66 36 453 4072
Fragments arranged by size:
4072 2811 453 66 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 3381 3864 928
Size: 971 242 1240 483 4502
Fragments arranged by size:
4502 1240 971 483 242
BbvCI CC'TCA_GC
Cuts at: 3775 4446 3775
Size: 671 6767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5629 5663 1903
Size: 34 3692 34 3678
Fragments arranged by size:
3692 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5330 6540 5330
Size: 1210 6228
BclI T'GATC_A
Cuts at: 1548 2037 1548
Size: 489 6949
BfrBI ATG'CAT
Cuts at: 2354 2354
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6049 136
Size: 107 122 71 631 605 4377 1525
Fragments arranged by size:
4377 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7433 7433
Size: 7438
BlpI GC'TnA_GC
Cuts at: 4855 4855
Size: 7438
Bme1580I G_kGCm'C
Cuts at: 1790 3654 4202 4268 4304 5499 6655 1790
Size: 1864 548 66 36 1195 1156 2573
Fragments arranged by size:
2573 1864 1195 1156 548 66 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3497 5169 6127
Size: 416 53 83 399 24 2080 1672 958
Cuts at: 6127 442
Size: 1753
Fragments arranged by size:
2080 1753 1672 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3825 3825
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2172 2193 2603 2654 6098 1239
Size: 933 21 410 51 3444 2579
Fragments arranged by size:
3444 2579 933 410 51 21
Bpu10I CC'TnA_GC
Cuts at: 3775 4446 3775
Size: 671 6767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3106 5563 6428 6726 3106
Size: 2457 865 298 3818
Fragments arranged by size:
3818 2457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4641 6101 882
Size: 617 3142 1460 2219
Fragments arranged by size:
3142 2219 1460 617
BsaAI yAC'GTr
Cuts at: 493 1204 4534 5229 493
Size: 711 3330 695 2702
Fragments arranged by size:
3330 2702 711 695
BsaBI GATnn'nnATC
Cuts at: 2775 7432 2775
Size: 4657 2781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3004 5245 5627 6262
Size: 53 83 186 936 1471 2241 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2241 1471 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4638 4668 1178
Size: 30 165 30 3235 30 3948
Fragments arranged by size:
3948 3235 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2699 3812 3830 4720 1186
Size: 1513 1113 18 890 3904
Fragments arranged by size:
3904 1513 1113 890 18
BseYI C'CCAG_C
Cuts at: 3745 4380 4721 6641 7137 3745
Size: 635 341 1920 496 4046
Fragments arranged by size:
4046 1920 635 496 341
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3753 4151 1163
Size: 573 2017 398 4450
Fragments arranged by size:
4450 2017 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4327 5499 5584 6655 4327
Size: 1172 85 1071 5110
Fragments arranged by size:
5110 1172 1071 85
BsmI GAATG_Cn'
Cuts at: 3536 4949 5042 3536
Size: 1413 93 5932
Fragments arranged by size:
5932 1413 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2548 2908 4610 2548
Size: 360 1702 5376
Fragments arranged by size:
5376 1702 360
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3654 4202 4268 4304 4327 4757 5499
Size: 399 1864 548 66 36 23 430 742
Cuts at: 5499 5584 6655 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1864 1071 742 548 430 399 85
66 36 23
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3537 4793 844
Size: 2693 1256 3489
Fragments arranged by size:
3489 2693 1256
BsrBI CCG'CTC
Cuts at: 1394 1823 5325 1394
Size: 429 3502 3507
Fragments arranged by size:
3507 3502 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4299 5933 6107 65
Size: 1183 3051 1634 174 1396
Fragments arranged by size:
3051 1634 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6082 6269 1700
Size: 110 4272 187 2869
Fragments arranged by size:
4272 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 5498 6510 5498
Size: 1012 6426
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 3596 3626 794
Size: 1853 949 30 4606
Fragments arranged by size:
4606 1853 949 30
BstBI TT'CG_AA
Cuts at: 6242 6242
Size: 7438
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7438
BstXI CCAn_nnnn'nTGG
Cuts at: 3928 4405 3928
Size: 477 6961
BstYI r'GATC_y
Cuts at: 1972 3261 4768 5135 5520 5537 6989 7075 7087
Size: 1289 1507 367 385 17 1452 86 12
Cuts at: 7087 7433 1972
Size: 346 1977
Fragments arranged by size:
1977 1507 1452 1289 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2660 3810 4207 2660
Size: 1150 397 5891
Fragments arranged by size:
5891 1150 397
BtgI C'CryG_G
Cuts at: 513 1065 2004 2243 3226 513
Size: 552 939 239 983 4725
Fragments arranged by size:
4725 983 939 552 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 4954 5748 5768 4954
Size: 794 20 6624
Fragments arranged by size:
6624 794 20
ClaI AT'CG_AT
Cuts at: 1554 2116 1554
Size: 562 6876
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3892 3927 529
Size: 35 3328 35 4040
Fragments arranged by size:
4040 3328 35 35
DraI TTT'AAA
Cuts at: 2989 4742 2989
Size: 1753 5685
DraIII CAC_nnn'GTG
Cuts at: 4502 4613 4502
Size: 111 7327
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6445 809
Size: 507 5129 1802
Fragments arranged by size:
5129 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3187 3631 3973
Size: 54 1425 50 275 18 1357 444 342
Cuts at: 3973 4885 5774 7403 8
Size: 912 889 1629 43
Fragments arranged by size:
1629 1425 1357 912 889 444 342 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2694 2836 3063 3987 4655 4660 5368 1353
Size: 1341 142 227 924 668 5 708 3423
Fragments arranged by size:
3423 1341 924 708 668 227 142 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2172 2193 2285 2603 2654 3100 3632
Size: 393 540 21 92 318 51 446 532
Cuts at: 3632 4436 4679 5501 6098 6885 7153 1239
Size: 804 243 822 597 787 268 1524
Fragments arranged by size:
1524 822 804 787 597 540 532 446
393 318 268 243 92 51 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3794 3794
Size: 7438
EcoO109I rG'GnC_Cy
Cuts at: 4265 4340 4265
Size: 75 7363
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 3790 4734 6585 1678
Size: 2112 944 1851 2531
Fragments arranged by size:
2531 2112 1851 944
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2813 2845 2908 2940 3104
Size: 32 135 32 1241 32 63 32 164
Cuts at: 3104 3136 3719 3749 3751 3781 3899 3931 4530
Size: 32 583 30 2 30 118 32 599
Cuts at: 4530 4562 4638 4670 4701 4733 6079 6111 6153
Size: 32 76 32 31 32 1346 32 42
Cuts at: 6153 6185 1373
Size: 32 2626
Fragments arranged by size:
2626 1346 1241 599 583 164 135 118
76 63 42 32 32 32 32 32
32 32 32 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2224 2496 3469 4782 5028 669
Size: 988 281 286 272 973 1313 246 3079
Fragments arranged by size:
3079 1313 988 973 286 281 272 246
HindIII A'AGCT_T
Cuts at: 748 2216 748
Size: 1468 5970
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 2157 5037 2157
Size: 2880 4558
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2353 2743 4534 4624 6552 6736 1305
Size: 1048 390 1791 90 1928 184 2007
Fragments arranged by size:
2007 1928 1791 1048 390 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3189 3633 3975 10
Size: 54 1425 343 1357 444 342 3473
Fragments arranged by size:
3473 1425 1357 444 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3965 3969 4403 5396 5755 5914 518
Size: 648 2799 4 434 993 359 159 2042
Fragments arranged by size:
2799 2042 993 648 434 359 159 4
MspA1I CmG'CkG
Cuts at: 1606 3353 3868 4216 4403 4725 4854 5531 6679
Size: 1747 515 348 187 322 129 677 1148
Cuts at: 6679 6924 1606
Size: 245 2120
Fragments arranged by size:
2120 1747 1148 677 515 348 322 245
187 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 2243 3226 513
Size: 552 939 239 983 4725
Fragments arranged by size:
4725 983 939 552 239
NdeI CA'TA_TG
Cuts at: 387 3184 387
Size: 2797 4641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NsiI A_TGCA'T
Cuts at: 2356 2356
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 3062 4796 6341 1132
Size: 97 1833 1734 1545 2229
Fragments arranged by size:
2229 1833 1734 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7438
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7438
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4340 4340
Size: 7438
PshAI GACnn'nnGTC
Cuts at: 1613 3700 1613
Size: 2087 5351
PsiI TTA'TAA
Cuts at: 2748 5008 2748
Size: 2260 5178
PspOMI G'GGCC_C
Cuts at: 4198 4264 4300 4198
Size: 66 36 7336
Fragments arranged by size:
7336 66 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2784 2816 2784
Size: 32 7406
PstI C_TGCA'G
Cuts at: 830 3534 3592 3690 3991 4144 4441 4790 830
Size: 2704 58 98 301 153 297 349 3478
Fragments arranged by size:
3478 2704 349 301 297 153 98 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 3353 4216 4403 3353
Size: 863 187 6388
Fragments arranged by size:
6388 863 187
SalI G'TCGA_C
Cuts at: 1936 4780 1936
Size: 2844 4594
SapI GCTCTTCn'nnn_
Cuts at: 2694 3063 2694
Size: 369 7069
SbfI CC_TGCA'GG
Cuts at: 3534 4144 4790 3534
Size: 610 646 6182
Fragments arranged by size:
6182 646 610
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2369 3358 3530 3588 3686 3987 4140
Size: 219 1324 989 172 58 98 301 153
Cuts at: 4140 4437 4786 5921 6602 6793 826
Size: 297 349 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 989 681 349 301 297
219 191 172 153 98 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 4765 1872
Size: 2893 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3121 5542 6443 6705 820
Size: 197 926 1178 2421 901 262 1553
Fragments arranged by size:
2421 1553 1178 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 3479 5362 5
Size: 47 3427 1883 2081
Fragments arranged by size:
3427 2081 1883 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7438
StyI C'CwwG_G
Cuts at: 513 1065 2004 2243 3226 4366 4642 4877 513
Size: 552 939 239 983 1140 276 235 3074
Fragments arranged by size:
3074 1140 983 939 552 276 239 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 3516 5465 5482 5635 5820 6265
Size: 1255 686 1250 1949 17 153 185 445
Cuts at: 6265 325
Size: 1498
Fragments arranged by size:
1949 1498 1255 1250 686 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4162 5684 7396
Size: 274 80 33 51 1443 2185 1522 1712
Cuts at: 7396 96
Size: 138
Fragments arranged by size:
2185 1712 1522 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7398 7430 7398
Size: 32 7406
Tth111I GACn'n_nGTC
Cuts at: 1316 4652 1316
Size: 3336 4102
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3093 3409 3093
Size: 316 7122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 4763 1870
Size: 2893 4545
XmnI GAAnn'nnTTC
Cuts at: 4048 5567 4048
Size: 1519 5919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI