Restriction map for
pFN21AB0443
(Circular) (Six-base) MAPSORT of: pFN21AB0443.seq Check: 2890 from: 1 to: 5821
pFN21AB0443 5821 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3005 3631 278
Size: 53 83 186 936 1469 626 2468
Fragments arranged by size:
2468 1469 936 626 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 2583 3142 2583
Size: 559 5262
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2165 2198 2509 2513 2861 3884 5268 5536
Size: 533 33 311 4 348 1023 1384 268
Cuts at: 5536 1632
Size: 1917
Fragments arranged by size:
1917 1384 1023 533 348 311 268 33
4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2349 1184
Size: 1165 4656
AlwNI CAG_nnn'CTG
Cuts at: 2401 3564 5136 2401
Size: 1163 1572 3086
Fragments arranged by size:
3086 1572 1163
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2606 3146 1870
Size: 73 574 89 540 4545
Fragments arranged by size:
4545 574 540 89 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2567 2600 2567
Size: 33 5788
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2087 2115 2583 3022 3142
Size: 325 217 627 300 28 468 439 120
Cuts at: 3142 4597 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 468 439 325 300 217
120 28
BanII G_rGCy'C
Cuts at: 1391 3140 1391
Size: 1749 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3109 928
Size: 971 1210 3640
Fragments arranged by size:
3640 1210 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3039 4432 136
Size: 107 122 71 631 605 1367 1393 1525
Fragments arranged by size:
1525 1393 1367 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3882 5038 1790
Size: 246 1846 1156 2573
Fragments arranged by size:
2573 1846 1156 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 4481 1239
Size: 1580 1662 2579
Fragments arranged by size:
2579 1662 1580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3946 4811 5109 2960
Size: 986 865 298 3672
Fragments arranged by size:
3672 986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 2207 5815 2207
Size: 3608 2213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2928 3002 3628 4010
Size: 53 83 186 936 1395 74 626 382
Cuts at: 4010 4645 275
Size: 635 1451
Fragments arranged by size:
1451 1395 936 635 626 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2336 2648 2907 3054 5024 5520 2336
Size: 312 259 147 1970 496 2637
Fragments arranged by size:
2637 1970 496 312 259 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 3140 3882 3967 5038 1391
Size: 399 246 1104 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 742 399 246 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3176 844
Size: 2332 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 3708 1394
Size: 429 791 1094 3507
Fragments arranged by size:
3507 1094 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 4316 4490 65
Size: 1183 1108 1960 174 1396
Fragments arranged by size:
1960 1396 1183 1108 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2586 4465 4652 1700
Size: 110 316 460 1879 187 2869
Fragments arranged by size:
2869 1879 460 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 2228 2478 3881 4893 2228
Size: 250 1403 1012 3156
Fragments arranged by size:
3156 1403 1012 250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2401 794
Size: 1607 4214
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 2569
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2208 2436 3151 3518 3903 3920 5372 5458
Size: 236 228 715 367 385 17 1452 86
Cuts at: 5458 5470 5816 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 715 385 367 346 236 228
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2754 2754
Size: 5821
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2937 487
Size: 683 56 1711 3371
Fragments arranged by size:
3371 1711 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2128 2532
Size: 54 1425 50 275 18 250 48 404
Cuts at: 2532 2762 3268 4157 5786 8
Size: 230 506 889 1629 43
Fragments arranged by size:
1629 1425 889 506 404 275 250 230
54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3751 1353
Size: 2398 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2198 2509 2513 2819 2861 3884
Size: 393 533 33 311 4 306 42 1023
Cuts at: 3884 4481 5268 5536 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1023 787 597 533 393 311 306
268 42 33 4
EcoO109I rG'GnC_Cy
Cuts at: 2488 2488
Size: 5821
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3064 3096 4462 4494 4536
Size: 32 135 32 1492 32 1366 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 1492 1366 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 3165 3411 669
Size: 988 281 304 923 246 3079
Fragments arranged by size:
3079 988 923 304 281 246
HindIII A'AGCT_T
Cuts at: 748 2268 748
Size: 1520 4301
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 2587 3146 2587
Size: 559 5262
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 2994 4935 5119 1305
Size: 1674 15 1941 184 2007
Fragments arranged by size:
2007 1941 1674 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 2764 10
Size: 54 1425 343 702 230 3067
Fragments arranged by size:
3067 1425 702 343 230 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 3779 4138 4297 518
Size: 648 1066 1547 359 159 2042
Fragments arranged by size:
2042 1547 1066 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2286 3237 3914 5062 5307 1606
Size: 680 951 677 1148 245 2120
Fragments arranged by size:
2120 1148 951 680 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 2986 3179 4724 1132
Size: 97 1757 193 1545 2229
Fragments arranged by size:
2229 1757 1545 193 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 1108
Size: 1029 4792
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2488
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 3017 4069 5781 3017
Size: 1052 1712 3057
Fragments arranged by size:
3057 1712 1052
SexAI A'CCwGG_T
Cuts at: 2304 2304
Size: 5821
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 2519 3148 1872
Size: 647 629 4545
Fragments arranged by size:
4545 647 629
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2975 3925 4826 5088 820
Size: 197 926 1032 950 901 262 1553
Fragments arranged by size:
1553 1032 950 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 2986 3179 1132
Size: 1854 193 3774
Fragments arranged by size:
3774 1854 193
SrfI GCCC'GGGC
Cuts at: 2519 2519
Size: 5821
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2846 1517
Size: 1329 4492
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 3260 513
Size: 552 939 79 1177 3074
Fragments arranged by size:
3074 1177 939 552 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2640 3008 3068 3848 3865 4018 4203
Size: 1255 1060 368 60 780 17 153 185
Cuts at: 4203 4648 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1060 780 445 368 185 153
60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3015 4067 5779
Size: 274 80 33 51 1443 1038 1052 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1052 1038 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 5781 5813 2987
Size: 32 2762 32 2995
Fragments arranged by size:
2995 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 2517 3146 1870
Size: 647 629 4545
Fragments arranged by size:
4545 647 629
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3003 3629 276
Size: 53 83 186 936 1469 626 2468
Fragments arranged by size:
2468 1469 936 626 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SwaI XcmI