Restriction map for
pFN21AB0437
(Circular) (Six-base) MAPSORT of: pFN21AB0437.seq Check: 7165 from: 1 to: 6148
pFN21AB0437 6148 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3491 1937
Size: 1554 4594
Acc65I G'GTAC_C
Cuts at: 2618 3469 2618
Size: 851 5297
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2841 2858 2907 2942 3143 3341 4211 5595
Size: 1209 17 49 35 201 198 870 1384
Cuts at: 5595 5863 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1209 870 268 201 198 49
35 17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 2826 5047 6024 1203
Size: 1623 2221 977 1327
Fragments arranged by size:
2221 1623 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 3078 3110 2318
Size: 32 728 32 5356
Fragments arranged by size:
5356 728 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 2460 2834 3044 3457 3683 3835 5944 1493
Size: 967 374 210 413 226 152 2109 1697
Fragments arranged by size:
2109 1697 967 413 374 226 210 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2143 3473 1870
Size: 73 200 1330 4545
Fragments arranged by size:
4545 1330 200 73
BamHI G'GATC_C
Cuts at: 2364 3478 3845 2364
Size: 1114 367 4667
Fragments arranged by size:
4667 1114 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 3380 3469 4924 618
Size: 325 217 627 831 762 89 1455 1842
Fragments arranged by size:
1842 1455 831 762 627 325 217 89
BanII G_rGCy'C
Cuts at: 1391 2425 2641 2882 3055 3467 1391
Size: 1034 216 241 173 412 4072
Fragments arranged by size:
4072 1034 412 241 216 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 928
Size: 971 930 4247
Fragments arranged by size:
4247 971 930
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3025 4040 5250 3025
Size: 1015 1210 3923
Fragments arranged by size:
3923 1210 1015
BclI T'GATC_A
Cuts at: 1548 3210 1548
Size: 1662 4486
BfrBI ATG'CAT
Cuts at: 3018 3018
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2842 3324 6143 2842
Size: 482 2819 2847
Fragments arranged by size:
2847 2819 482
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 3383 4209 5365 1790
Size: 1593 826 1156 2573
Fragments arranged by size:
2573 1593 1156 826
BmgBI CAC'GTC
Cuts at: 1318 2827 2907 1318
Size: 1509 80 4559
Fragments arranged by size:
4559 1509 80
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 3386 3879
Size: 416 53 83 399 24 758 1211 493
Cuts at: 3879 4837 442
Size: 958 1753
Fragments arranged by size:
1753 1211 958 758 493 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2439 3111 3387 4808 1239
Size: 866 334 672 276 1421 2579
Fragments arranged by size:
2579 1421 866 672 334 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2994 4811 882
Size: 617 1495 1817 2219
Fragments arranged by size:
2219 1817 1495 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2048 2063 2078 1178
Size: 30 165 30 630 15 15 15 5248
Fragments arranged by size:
5248 630 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2108 2948 1186
Size: 897 25 840 4386
Fragments arranged by size:
4386 897 840 25
BseYI C'CCAG_C
Cuts at: 5351 5847 5351
Size: 496 5652
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2239 2464 2709 1163
Size: 573 503 225 245 4602
Fragments arranged by size:
4602 573 503 245 225
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2641 3055 3082 4209 4294 5365 2641
Size: 414 27 1127 85 1071 3424
Fragments arranged by size:
3424 1127 1071 414 85 27
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 2641 2882 3055 3082 3383 3467
Size: 399 635 216 241 173 27 301 84
Cuts at: 3467 4209 4294 5365 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 399 301 241 216
173 85 84 27
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 3503 844
Size: 2299 360 3489
Fragments arranged by size:
3489 2299 360
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2677 2944 4643 4817 65
Size: 1183 1429 267 1699 174 1396
Fragments arranged by size:
1699 1429 1396 1183 267 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3353 4792 4979 1700
Size: 110 1543 1439 187 2869
Fragments arranged by size:
2869 1543 1439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2113 2364 2842 3324 3478 3845 4230 4247
Size: 141 251 478 482 154 367 385 17
Cuts at: 4247 5699 5785 5797 6143 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 482 478 385 367 346 251
154 141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2763 487
Size: 683 56 1537 3872
Fragments arranged by size:
3872 1537 683 56
BtsI GCAGTG_nn'
Cuts at: 2697 3364 3664 4458 4478 2697
Size: 667 300 794 20 4367
Fragments arranged by size:
4367 794 667 300 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DraIII CAC_nnn'GTG
Cuts at: 2442 2455 2442
Size: 13 6135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 5155 809
Size: 507 876 2963 1802
Fragments arranged by size:
2963 1802 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2479 3348 3595
Size: 54 1425 50 275 18 649 869 247
Cuts at: 3595 4484 6113 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 869 649 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 2345 2877 4078 1353
Size: 748 244 532 1201 3423
Fragments arranged by size:
3423 1201 748 532 244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2639 3053 2639
Size: 414 5734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2439 2841 2858 2907 2942 3111
Size: 393 473 334 402 17 49 35 169
Cuts at: 3111 3143 3341 3387 4211 4808 5595 5863 1239
Size: 32 198 46 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 597 473 402 393 334
268 198 169 49 46 35 32 17
EcoO109I rG'GnC_Cy
Cuts at: 2140 3086 2140
Size: 946 5202
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2864 2864
Size: 6148
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2375 2407 4789
Size: 32 135 32 461 32 310 32 2382
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2382 461 310 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2889 748
Size: 2141 4007
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 2622 3473 2622
Size: 851 5297
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 5262 5446 1305
Size: 996 2961 184 2007
Fragments arranged by size:
2961 2007 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2481 10
Size: 54 1425 343 649 3677
Fragments arranged by size:
3677 1425 649 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3231 4106 4465 4624 518
Size: 648 2065 875 359 159 2042
Fragments arranged by size:
2065 2042 875 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2375 3397 3564 4241 5389 5634 1606
Size: 498 271 1022 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 498 271 245 167
NaeI GCC'GGC
Cuts at: 3355 3355
Size: 6148
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 3020 3205 387
Size: 2633 185 3330
Fragments arranged by size:
3330 2633 185
NgoMIV G'CCGG_C
Cuts at: 3353 3353
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 3020 3020
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2308 2518 3506 5051 1132
Size: 97 1079 210 988 1545 2229
Fragments arranged by size:
2229 1545 1079 988 210 97
PasI CC'CwG_GG
Cuts at: 1348 2069 1348
Size: 721 5427
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2459 2491 4217 4249 5730 5762 2459
Size: 32 1726 32 1481 32 2845
Fragments arranged by size:
2845 1726 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 3086 2140
Size: 946 5202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2707 3500 830
Size: 1877 793 3478
Fragments arranged by size:
3478 1877 793
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2104 2375 2104
Size: 271 5877
SacI G_AGCT'C
Cuts at: 2641 3055 2641
Size: 414 5734
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 2877 2877
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2288 2703 3496 4631 5312 5503 826
Size: 219 1243 415 793 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 793 681 415 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 2015 1517
Size: 498 5650
StyI C'CwwG_G
Cuts at: 513 1065 3587 513
Size: 552 2522 3074
Fragments arranged by size:
3074 2522 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4175 4192 4345 4530 4975 325
Size: 1255 2595 17 153 185 445 1498
Fragments arranged by size:
2595 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2048 2080 6108 6140 2048
Size: 32 4028 32 2056
Fragments arranged by size:
4028 2056 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 2560 3484 2560
Size: 924 5224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2844 2871 4277 2844
Size: 27 1406 4715
Fragments arranged by size:
4715 1406 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI KasI NarI NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI