Restriction map for
pFN21AB0434
(Circular) (Six-base) MAPSORT of: pFN21AB0434.seq Check: 1068 from: 1 to: 7303
pFN21AB0434 7303 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4646 1937
Size: 2709 4594
Acc65I G'GTAC_C
Cuts at: 4624 4624
Size: 7303
AclI AA'CG_TT
Cuts at: 1563 5430 5803 1563
Size: 3867 373 3063
Fragments arranged by size:
3867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2411 3408 4602 5366 6750 7018 1632
Size: 623 156 997 1194 764 1384 268 1917
Fragments arranged by size:
1917 1384 1194 997 764 623 268 156
AflII C'TTAA_G
Cuts at: 820 1017 2353 3518 820
Size: 197 1336 1165 4605
Fragments arranged by size:
4605 1336 1165 197
AflIII A'CryG_T
Cuts at: 1203 3762 4406 6202 7179 1203
Size: 2559 644 1796 977 1327
Fragments arranged by size:
2559 1796 1327 977 644
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7303
AlwNI CAG_nnn'CTG
Cuts at: 4580 5046 6618 4580
Size: 466 1572 5265
Fragments arranged by size:
5265 1572 466
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 2096 2126 2522 2838 2953 3436 3503 3980
Size: 603 30 396 316 115 483 67 477
Cuts at: 3980 4249 4276 4470 4612 4838 4990 7099 1493
Size: 269 27 194 142 226 152 2109 1697
Fragments arranged by size:
2109 1697 603 483 477 396 316 269
226 194 152 142 115 67 30 27
AseI AT'TA_AT
Cuts at: 160 1032 3224 5857 160
Size: 872 2192 2633 1606
Fragments arranged by size:
2633 2192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 4628 1870
Size: 73 2685 4545
Fragments arranged by size:
4545 2685 73
AvrII C'CTAG_G
Cuts at: 4444 4444
Size: 7303
BamHI G'GATC_C
Cuts at: 4633 5000 4633
Size: 367 6936
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 4624 6079 618
Size: 325 217 627 1091 1746 1455 1842
Fragments arranged by size:
1842 1746 1455 1091 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3811 4622 1391
Size: 2420 811 4072
Fragments arranged by size:
4072 2420 811
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2971 928
Size: 971 1072 5260
Fragments arranged by size:
5260 1072 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2699 2733 5494 5528 1903
Size: 34 50 34 678 34 2761 34 3678
Fragments arranged by size:
3678 2761 678 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2887 5914
Size: 107 122 71 631 605 337 878 3027
Cuts at: 5914 136
Size: 1525
Fragments arranged by size:
3027 1525 878 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2389 3693 7298 2389
Size: 1304 3605 2394
Fragments arranged by size:
3605 2394 1304
BlpI GC'TnA_GC
Cuts at: 4720 4720
Size: 7303
Bme1580I G_kGCm'C
Cuts at: 1790 3481 5364 6520 1790
Size: 1691 1883 1156 2573
Fragments arranged by size:
2573 1883 1691 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2785 5034 5992
Size: 416 53 83 399 24 1368 2249 958
Cuts at: 5992 442
Size: 1753
Fragments arranged by size:
2249 1753 1368 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4385 5963 1239
Size: 3146 1578 2579
Fragments arranged by size:
3146 2579 1578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2414 2792 5428 6293 6591 2077
Size: 337 378 2636 865 298 2789
Fragments arranged by size:
2789 2636 865 378 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2621 5966 882
Size: 617 1122 3345 2219
Fragments arranged by size:
3345 2219 1122 617
BsaAI yAC'GTr
Cuts at: 493 1204 3791 5094 493
Size: 711 2587 1303 2702
Fragments arranged by size:
2702 2587 1303 711
BsaBI GATnn'nnATC
Cuts at: 7297 7297
Size: 7303
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5110 5492 6127 275
Size: 53 83 186 936 3577 382 635 1451
Fragments arranged by size:
3577 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5736 6134 6408 6555 6966 5736
Size: 398 274 147 411 6073
Fragments arranged by size:
6073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7078
Fragments arranged by size:
7078 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3917 1186
Size: 2731 4572
BseYI C'CCAG_C
Cuts at: 6506 7002 6506
Size: 496 6807
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6730
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4753 5514 6542 1540
Size: 275 186 12 2740 761 1028 2301
Fragments arranged by size:
2740 2301 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 3401 3811 5364 5449 6520 3401
Size: 410 1553 85 1071 4184
Fragments arranged by size:
4184 1553 1071 410 85
BsmI GAATG_Cn'
Cuts at: 2041 4814 4907 2041
Size: 2773 93 4437
Fragments arranged by size:
4437 2773 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4151 4151
Size: 7303
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3401 3481 3811 4622 5364 5449 6520
Size: 399 1611 80 330 811 742 85 1071
Cuts at: 6520 1391
Size: 2174
Fragments arranged by size:
2174 1611 1071 811 742 399 330 85
80
BspEI T'CCGG_A
Cuts at: 6966 6966
Size: 7303
BspHI T'CATG_A
Cuts at: 3529 3709 4129 5192 6922 3529
Size: 180 420 1063 1730 3910
Fragments arranged by size:
3910 1730 1063 420 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 2603 4658 844
Size: 1602 157 2055 3489
Fragments arranged by size:
3489 2055 1602 157
BsrBI CCG'CTC
Cuts at: 1394 1823 5190 1394
Size: 429 3367 3507
Fragments arranged by size:
3507 3367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3501 4286 5798 5972 65
Size: 1183 2253 785 1512 174 1396
Fragments arranged by size:
2253 1512 1396 1183 785 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 3072 5947 6134 1700
Size: 110 198 1064 2875 187 2869
Fragments arranged by size:
2875 2869 1064 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7303
BssSI C'ACGA_G
Cuts at: 3239 5363 6375 3239
Size: 2124 1012 4167
Fragments arranged by size:
4167 2124 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2967 3683 794
Size: 2173 716 4414
Fragments arranged by size:
4414 2173 716
BstBI TT'CG_AA
Cuts at: 6107 6107
Size: 7303
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7303
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 2875
Size: 7303
BstYI r'GATC_y
Cuts at: 1972 2052 2389 3693 4330 4633 5000 5385 5402
Size: 80 337 1304 637 303 367 385 17
Cuts at: 5402 6854 6940 6952 7298 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1304 637 385 367 346 337
303 86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2945 2945
Size: 7303
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6751
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6564
Fragments arranged by size:
6564 683 56
BtsI GCAGTG_nn'
Cuts at: 3458 4167 4819 5613 5633 3458
Size: 709 652 794 20 5128
Fragments arranged by size:
5128 794 709 652 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7268
DraI TTT'AAA
Cuts at: 2923 4607 2923
Size: 1684 5619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6310 809
Size: 507 4994 1802
Fragments arranged by size:
4994 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3945 4590
Size: 54 1425 50 275 18 180 1935 645
Cuts at: 4590 4750 5639 7268 8
Size: 160 889 1629 43
Fragments arranged by size:
1935 1629 1425 889 645 275 180 160
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 4750 1537
Size: 275 198 2740 4090
Fragments arranged by size:
4090 2740 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2231 3027 3799 3828 4530 5233 1353
Size: 695 183 796 772 29 702 703 3423
Fragments arranged by size:
3423 796 772 703 702 695 183 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5890 6264 6410 387
Size: 1064 138 4301 374 146 1280
Fragments arranged by size:
4301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3809 3809
Size: 7303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2411 3408 4385 4602 5366 5963
Size: 393 623 156 997 977 217 764 597
Cuts at: 5963 6750 7018 1239
Size: 787 268 1524
Fragments arranged by size:
1524 997 977 787 764 623 597 393
268 217 156
EcoO109I rG'GnC_Cy
Cuts at: 2359 2359
Size: 7303
EcoRI G'AATT_C
Cuts at: 4276 4612 4990 4276
Size: 336 378 6589
Fragments arranged by size:
6589 378 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2360 2717 3662 3944 2071
Size: 289 357 945 282 5430
Fragments arranged by size:
5430 945 357 289 282
FspI TGC'GCA
Cuts at: 2442 5809 2442
Size: 3367 3936
HaeII r_GCGC'y
Cuts at: 1678 6450 1678
Size: 4772 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2848 2880 3898 3930 4009
Size: 32 135 32 1276 32 1018 32 79
Cuts at: 4009 4041 5944 5976 6018 6050 1373
Size: 32 1903 32 42 32 2626
Fragments arranged by size:
2626 1903 1276 1018 135 79 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4647 4893 669
Size: 988 281 2709 246 3079
Fragments arranged by size:
3079 2709 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2196 2628 3704 4493 748
Size: 1448 432 1076 789 3558
Fragments arranged by size:
3558 1448 1076 789 432
HpaI GTT'AAC
Cuts at: 4893 4893
Size: 7303
KpnI G_GTAC'C
Cuts at: 4628 4628
Size: 7303
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6417 6601 1305
Size: 5112 184 2007
Fragments arranged by size:
5112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3947 4592 10
Size: 54 1425 343 2115 645 2721
Fragments arranged by size:
2721 2115 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 2326 5261 5620 5779 518
Size: 648 1093 67 2935 359 159 2042
Fragments arranged by size:
2935 2042 1093 648 359 159 67
MspA1I CmG'CkG
Cuts at: 1606 3428 4307 4719 5396 6544 6789 1606
Size: 1822 879 412 677 1148 245 2120
Fragments arranged by size:
2120 1822 1148 879 677 412 245
NaeI GCC'GGC
Cuts at: 2010 2010
Size: 7303
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6751
NdeI CA'TA_TG
Cuts at: 387 3616 387
Size: 3229 4074
NgoMIV G'CCGG_C
Cuts at: 2008 2008
Size: 7303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 3266 3361 3460 3766 4410 4661 6206
Size: 97 2037 95 99 306 644 251 1545
Cuts at: 6206 1132
Size: 2229
Fragments arranged by size:
2229 2037 1545 644 306 251 99 97
95
PasI CC'CwG_GG
Cuts at: 1348 2868 1348
Size: 1520 5783
PciI A'CATG_T
Cuts at: 3762 4406 6202 3762
Size: 644 1796 4863
Fragments arranged by size:
4863 1796 644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3313 1108
Size: 2205 5098
PfoI T'CCnGG_A
Cuts at: 4525 4525
Size: 7303
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3222 3254 5372 5404 6885 6917 3222
Size: 32 2118 32 1481 32 3608
Fragments arranged by size:
3608 2118 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 7303
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7303
PsiI TTA'TAA
Cuts at: 3489 4873 3489
Size: 1384 5919
PstI C_TGCA'G
Cuts at: 830 2737 4162 4576 4655 830
Size: 1907 1425 414 79 3478
Fragments arranged by size:
3478 1907 1425 414 79
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 4307 4307
Size: 7303
SacI G_AGCT'C
Cuts at: 3811 3811
Size: 7303
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SapI GCTCTTCn'nnn_
Cuts at: 2048 3799 2048
Size: 1751 5552
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 3137 5551 7263 3137
Size: 2414 1712 3177
Fragments arranged by size:
3177 2414 1712
SfcI C'TryA_G
Cuts at: 826 1045 2372 2620 2679 2733 3687 4158 4353
Size: 219 1327 248 59 54 954 471 195
Cuts at: 4353 4572 4651 5786 6467 6658 826
Size: 219 79 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 954 681 471 248 219
219 195 191 79 59 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 4630 1872
Size: 2758 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 2353 2393 2807 3518 5407
Size: 197 926 113 297 40 414 711 1889
Cuts at: 5407 6308 6570 820
Size: 901 262 1553
Fragments arranged by size:
1889 1553 926 901 711 414 297 262
197 113 40
SnaBI TAC'GTA
Cuts at: 493 3791 5094 493
Size: 3298 1303 2702
Fragments arranged by size:
3298 2702 1303
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7303
SphI G_CATG'C
Cuts at: 1132 3266 4661 1132
Size: 2134 1395 3774
Fragments arranged by size:
3774 2134 1395
SspI AAT'ATT
Cuts at: 5 52 3274 5227 5
Size: 47 3222 1953 2081
Fragments arranged by size:
3222 2081 1953 47
StuI AGG'CCT
Cuts at: 1517 3311 1517
Size: 1794 5509
StyI C'CwwG_G
Cuts at: 513 1065 2028 2148 3312 3948 4444 4742 513
Size: 552 963 120 1164 636 496 298 3074
Fragments arranged by size:
3074 1164 963 636 552 496 298 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5330 5347 5500 5685 6130 325
Size: 1255 3750 17 153 185 445 1498
Fragments arranged by size:
3750 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3135 3189 3212
Size: 274 80 33 51 1443 1158 54 23
Cuts at: 3212 3643 5549 7261 96
Size: 431 1906 1712 138
Fragments arranged by size:
1906 1712 1443 1158 431 274 138 80
54 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7263 7295 7263
Size: 32 7271
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7303
XbaI T'CTAG_A
Cuts at: 4639 4639
Size: 7303
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7303
XmaI C'CCGG_G
Cuts at: 1870 4628 1870
Size: 2758 4545
XmnI GAAnn'nnTTC
Cuts at: 2208 3234 3930 4065 4263 5432 2208
Size: 1026 696 135 198 1169 4079
Fragments arranged by size:
4079 1169 1026 696 198 135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI