Restriction map for
pFN21AB0433
(Circular) (Six-base) MAPSORT of: pFN21AB0433.seq Check: 5668 from: 1 to: 6073
pFN21AB0433 6073 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3883 278
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2026 3416 1937
Size: 89 1390 4594
Fragments arranged by size:
4594 1390 89
Acc65I G'GTAC_C
Cuts at: 3394 3394
Size: 6073
AclI AA'CG_TT
Cuts at: 1563 4200 4573 1563
Size: 2637 373 3063
Fragments arranged by size:
3063 2637 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3061 4136 5520 5788 1632
Size: 407 1022 1075 1384 268 1917
Fragments arranged by size:
1917 1384 1075 1022 407 268
AfeI AGC'GCT
Cuts at: 2239 2239
Size: 6073
AflII C'TTAA_G
Cuts at: 820 1017 2806 2945 820
Size: 197 1789 139 3948
Fragments arranged by size:
3948 1789 197 139
AflIII A'CryG_T
Cuts at: 1203 4972 5949 1203
Size: 3769 977 1327
Fragments arranged by size:
3769 1327 977
AgeI A'CCGG_T
Cuts at: 4904 4904
Size: 6073
AhdI GACnn_n'nnGTC
Cuts at: 4802 4802
Size: 6073
AleI CACnn'nnGTG
Cuts at: 1166 2020 1166
Size: 854 5219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2315 1184
Size: 1131 4942
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3092 3124 3092
Size: 32 6041
AlwNI CAG_nnn'CTG
Cuts at: 2203 2591 2714 3300 3816 5388 2203
Size: 388 123 586 516 1572 2888
Fragments arranged by size:
2888 1572 586 516 388 123
ApaI G_GGCC'C
Cuts at: 2247 2247
Size: 6073
ApaLI G'TGCA_C
Cuts at: 4130 5286 4130
Size: 1156 4917
ApoI r'AATT_y
Cuts at: 1493 2351 3382 3608 3760 5869 1493
Size: 858 1031 226 152 2109 1697
Fragments arranged by size:
2109 1697 1031 858 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4627 160
Size: 872 3595 1606
Fragments arranged by size:
3595 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 3129 3398 1870
Size: 73 133 1053 269 4545
Fragments arranged by size:
4545 1053 269 133 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3044 3077 3044
Size: 33 6040
BamHI G'GATC_C
Cuts at: 3358 3403 3770 3358
Size: 45 367 5661
Fragments arranged by size:
5661 367 45
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2213 3394 4849 618
Size: 325 217 627 259 167 1181 1455 1842
Fragments arranged by size:
1842 1455 1181 627 325 259 217 167
BanII G_rGCy'C
Cuts at: 1391 2247 2542 3392 1391
Size: 856 295 850 4072
Fragments arranged by size:
4072 856 850 295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4264 4298 1903
Size: 34 2327 34 3678
Fragments arranged by size:
3678 2327 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3965 5175 3965
Size: 1210 4863
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6073
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3264 4684
Size: 107 122 71 631 605 337 1255 1420
Cuts at: 4684 136
Size: 1525
Fragments arranged by size:
1525 1420 1255 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3307 6068 3307
Size: 2761 3312
BlpI GC'TnA_GC
Cuts at: 3490 3490
Size: 6073
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2218 2247 2913 3231 3289 4134 5290
Size: 261 167 29 666 318 58 845 1156
Cuts at: 5290 1790
Size: 2573
Fragments arranged by size:
2573 1156 845 666 318 261 167 58
29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2212 3019 3804
Size: 416 53 83 399 24 795 807 785
Cuts at: 3804 4762 442
Size: 958 1753
Fragments arranged by size:
1753 958 807 795 785 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2308 2500 3123 4733 1239
Size: 1069 192 623 1610 2579
Fragments arranged by size:
2579 1610 1069 623 192
Bpu10I CC'TnA_GC
Cuts at: 2584 3354 2584
Size: 770 5303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 4198 5063 5361 2363
Size: 1835 865 298 3075
Fragments arranged by size:
3075 1835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3141 4736 882
Size: 617 1642 1595 2219
Fragments arranged by size:
2219 1642 1595 617
BsaAI yAC'GTr
Cuts at: 493 1204 2185 2420 2639 3864 493
Size: 711 981 235 219 1225 2702
Fragments arranged by size:
2702 1225 981 711 235 219
BsaBI GATnn'nnATC
Cuts at: 3276 6067 3276
Size: 2791 3282
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3880 4262 4897 275
Size: 53 83 186 936 2347 382 635 1451
Fragments arranged by size:
2347 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2126 4506 4904 5178 5325 5736 2126
Size: 2380 398 274 147 411 2463
Fragments arranged by size:
2463 2380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5848
Fragments arranged by size:
5848 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 2399 3158 1186
Size: 1144 69 759 4101
Fragments arranged by size:
4101 1144 759 69
BseYI C'CCAG_C
Cuts at: 2240 2819 2906 5276 5772 2240
Size: 579 87 2370 496 2541
Fragments arranged by size:
2541 2370 579 496 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2795 1163
Size: 573 1059 4441
Fragments arranged by size:
4441 1059 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3523 4284 5312 1540
Size: 275 186 1522 761 1028 2301
Fragments arranged by size:
2301 1522 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3249 4134 4219 5290 3249
Size: 885 85 1071 4032
Fragments arranged by size:
4032 1071 885 85
BsmI GAATG_Cn'
Cuts at: 2522 3584 3677 2522
Size: 1062 93 4918
Fragments arranged by size:
4918 1062 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2218 2247 2542 2913 3231 3249
Size: 399 261 167 29 295 371 318 18
Cuts at: 3249 3289 3392 4134 4219 5290 1391
Size: 40 103 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 371 318 295 261
167 103 85 40 29 18
BspEI T'CCGG_A
Cuts at: 5736 5736
Size: 6073
BspHI T'CATG_A
Cuts at: 3962 5692 3962
Size: 1730 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3428 844
Size: 2584 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3960 1394
Size: 429 2137 3507
Fragments arranged by size:
3507 2137 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4568 4742 65
Size: 1183 3320 174 1396
Fragments arranged by size:
3320 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3258 4717 4904 1700
Size: 110 1448 1459 187 2869
Fragments arranged by size:
2869 1459 1448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6073
BssSI C'ACGA_G
Cuts at: 4133 5145 4133
Size: 1012 5061
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3244 794
Size: 2450 3623
BstBI TT'CG_AA
Cuts at: 4877 4877
Size: 6073
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6073
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 2938 2162
Size: 776 5297
BstYI r'GATC_y
Cuts at: 1972 2532 2935 3307 3358 3403 3770 4155 4172
Size: 560 403 372 51 45 367 385 17
Cuts at: 4172 5624 5710 5722 6068 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 560 403 385 372 367 346
86 51 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2135 3137 487
Size: 683 56 909 1002 3423
Fragments arranged by size:
3423 1002 909 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 3589 4383 4403 2409
Size: 1180 794 20 4079
Fragments arranged by size:
4079 1180 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6073
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2550 2585 529
Size: 35 1986 35 4017
Fragments arranged by size:
4017 1986 35 35
DraI TTT'AAA
Cuts at: 3377 3377
Size: 6073
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5080 809
Size: 507 3764 1802
Fragments arranged by size:
3764 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038
Size: 54 1425 50 275 18 1690 889 1629
Cuts at: 6038 8
Size: 43
Fragments arranged by size:
1690 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3520 1537
Size: 275 1708 4090
Fragments arranged by size:
4090 1708 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 4003 1353
Size: 782 1868 3423
Fragments arranged by size:
3423 1868 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4660 5034 5180 387
Size: 1064 138 3071 374 146 1280
Fragments arranged by size:
3071 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2308 2500 3061 3123 4136 4733
Size: 393 407 269 192 561 62 1013 597
Cuts at: 4733 5520 5788 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1013 787 597 561 407 393 269
268 192 62
EcoRI G'AATT_C
Cuts at: 3382 3760 3382
Size: 378 5695
FspI TGC'GCA
Cuts at: 4579 4579
Size: 6073
HaeII r_GCGC'y
Cuts at: 1678 2084 2241 5220 1678
Size: 406 157 2979 2531
Fragments arranged by size:
2979 2531 406 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2134 2140 2166 2266
Size: 32 135 32 536 26 6 26 100
Cuts at: 2266 2298 2524 2556 2567 2599 3112 3144 4714
Size: 32 226 32 11 32 513 32 1570
Cuts at: 4714 4746 4788 4820 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1570 536 513 226 135 100 42
32 32 32 32 32 32 32 32
26 26 11 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3417 3663 669
Size: 988 281 1092 387 246 3079
Fragments arranged by size:
3079 1092 988 387 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6073
HpaI GTT'AAC
Cuts at: 3663 3663
Size: 6073
KpnI G_GTAC'C
Cuts at: 3398 3398
Size: 6073
MfeI C'AATT_G
Cuts at: 3672 3672
Size: 6073
MluI A'CGCG_T
Cuts at: 5949 5949
Size: 6073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5187 5371 1305
Size: 3882 184 2007
Fragments arranged by size:
3882 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4251
Fragments arranged by size:
4251 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2020 4031 4390 4549 518
Size: 648 854 2011 359 159 2042
Fragments arranged by size:
2042 2011 854 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2666 3081 3489 4166 5314 5559 1606
Size: 1060 415 408 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 677 415 408 245
NaeI GCC'GGC
Cuts at: 3260 3260
Size: 6073
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4582
Fragments arranged by size:
4582 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6073
NgoMIV G'CCGG_C
Cuts at: 3258 3258
Size: 6073
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6073
NotI GC'GGCC_GC
Cuts at: 3520 3520
Size: 6073
NspI r_CATG'y
Cuts at: 1132 1229 3235 3242 3431 4976 1132
Size: 97 2006 7 189 1545 2229
Fragments arranged by size:
2229 2006 1545 189 97 7
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6073
PciI A'CATG_T
Cuts at: 4972 4972
Size: 6073
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6073
PfoI T'CCnGG_A
Cuts at: 2355 2355
Size: 6073
PmeI GTTT'AAAC
Cuts at: 3377 3377
Size: 6073
PmlI CAC'GTG
Cuts at: 2185 2185
Size: 6073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5655 5687 4142
Size: 32 1481 32 4528
Fragments arranged by size:
4528 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6073
PsiI TTA'TAA
Cuts at: 3643 3643
Size: 6073
PspOMI G'GGCC_C
Cuts at: 2243 2243
Size: 6073
PstI C_TGCA'G
Cuts at: 830 2139 3425 830
Size: 1309 1286 3478
Fragments arranged by size:
3478 1309 1286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6073
PvuII CAG'CTG
Cuts at: 2666 3081 2666
Size: 415 5658
SalI G'TCGA_C
Cuts at: 1936 3415 1936
Size: 1479 4594
SbfI CC_TGCA'GG
Cuts at: 3425 3425
Size: 6073
ScaI AGT'ACT
Cuts at: 4321 6033 4321
Size: 1712 4361
SexAI A'CCwGG_T
Cuts at: 2980 2980
Size: 6073
SfcI C'TryA_G
Cuts at: 826 1045 2135 2304 3043 3421 4556 5237 5428
Size: 219 1090 169 739 378 1135 681 191
Cuts at: 5428 826
Size: 1471
Fragments arranged by size:
1471 1135 1090 739 681 378 219 191
169
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6073
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6073
SmaI CCC'GGG
Cuts at: 1872 2078 3400 1872
Size: 206 1322 4545
Fragments arranged by size:
4545 1322 206
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2806 2945 4177 5078 5340
Size: 197 926 399 464 139 1232 901 262
Cuts at: 5340 820
Size: 1553
Fragments arranged by size:
1553 1232 926 901 464 399 262 197
139
SnaBI TAC'GTA
Cuts at: 493 2420 3864 493
Size: 1927 1444 2702
Fragments arranged by size:
2702 1927 1444
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6073
SphI G_CATG'C
Cuts at: 1132 3242 3431 1132
Size: 2110 189 3774
Fragments arranged by size:
3774 2110 189
SrfI GCCC'GGGC
Cuts at: 2078 2078
Size: 6073
SspI AAT'ATT
Cuts at: 5 52 3997 5
Size: 47 3945 2081
Fragments arranged by size:
3945 2081 47
StuI AGG'CCT
Cuts at: 1517 2146 1517
Size: 629 5444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2056 2769 3512 513
Size: 552 939 52 713 743 3074
Fragments arranged by size:
3074 939 743 713 552 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2782 4100 4117 4270 4455 4900 325
Size: 1255 1202 1318 17 153 185 445 1498
Fragments arranged by size:
1498 1318 1255 1202 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4319 6031 96
Size: 274 80 33 51 1443 2342 1712 138
Fragments arranged by size:
2342 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6033 6065 6033
Size: 32 6041
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6073
XbaI T'CTAG_A
Cuts at: 3409 3409
Size: 6073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 2677
Size: 6073
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6073
XmaI C'CCGG_G
Cuts at: 1870 2076 3398 1870
Size: 206 1322 4545
Fragments arranged by size:
4545 1322 206
XmnI GAAnn'nnTTC
Cuts at: 2351 4202 2351
Size: 1851 4222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3881 276
Size: 53 83 186 936 2347 2468
Fragments arranged by size:
2468 2347 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SwaI