Restriction map for
pFN21AB0432
(Circular) (Six-base) MAPSORT of: pFN21AB0432.seq Check: 9254 from: 1 to: 7267
pFN21AB0432 7267 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3304 3406 3687 3304
Size: 102 281 6884
Fragments arranged by size:
6884 281 102
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5077 278
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3621 4548 4610 1937
Size: 1684 927 62 4594
Fragments arranged by size:
4594 1684 927 62
Acc65I G'GTAC_C
Cuts at: 4453 4588 4453
Size: 135 7132
AclI AA'CG_TT
Cuts at: 1563 5394 5767 1563
Size: 3831 373 3063
Fragments arranged by size:
3831 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2921 3935 4034 4063 4352 5330 6714 6982
Size: 1289 1014 99 29 289 978 1384 268
Cuts at: 6982 1632
Size: 1917
Fragments arranged by size:
1917 1384 1289 1014 978 289 268 99
29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7070
AflIII A'CryG_T
Cuts at: 1203 3924 6166 7143 1203
Size: 2721 2242 977 1327
Fragments arranged by size:
2721 2242 1327 977
AgeI A'CCGG_T
Cuts at: 3038 6098 3038
Size: 3060 4207
AhdI GACnn_n'nnGTC
Cuts at: 5996 5996
Size: 7267
AleI CACnn'nnGTG
Cuts at: 1166 3039 3144 3855 1166
Size: 1873 105 711 4578
Fragments arranged by size:
4578 1873 711 105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7267
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2495 2527 2495
Size: 32 7235
AlwNI CAG_nnn'CTG
Cuts at: 2096 2389 2614 2716 3241 4346 5010 6582 2096
Size: 293 225 102 525 1105 664 1572 2781
Fragments arranged by size:
2781 1572 1105 664 525 293 225 102
ApaI G_GGCC'C
Cuts at: 2480 2621 4523 2480
Size: 141 1902 5224
Fragments arranged by size:
5224 1902 141
ApaLI G'TGCA_C
Cuts at: 3563 5324 6480 3563
Size: 1761 1156 4350
Fragments arranged by size:
4350 1761 1156
ApoI r'AATT_y
Cuts at: 1493 3362 4576 4802 4954 7063 1493
Size: 1869 1214 226 152 2109 1697
Fragments arranged by size:
2109 1869 1697 1214 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5821 160
Size: 872 4789 1606
Fragments arranged by size:
4789 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
AvaI C'yCGr_G
Cuts at: 1870 1943 3853 4358 4592 1870
Size: 73 1910 505 234 4545
Fragments arranged by size:
4545 1910 505 234 73
BamHI G'GATC_C
Cuts at: 4597 4964 4597
Size: 367 6900
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 2921 3149 4354 4453
Size: 325 217 627 959 175 228 1205 99
Cuts at: 4453 4588 6043 618
Size: 135 1455 1842
Fragments arranged by size:
1842 1455 1205 959 627 325 228 217
175 135 99
BanII G_rGCy'C
Cuts at: 1391 2480 2621 3942 4481 4523 4586 1391
Size: 1089 141 1321 539 42 63 4072
Fragments arranged by size:
4072 1321 1089 539 141 63 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 928
Size: 971 1010 5286
Fragments arranged by size:
5286 1010 971
BbvCI CC'TCA_GC
Cuts at: 2586 4467 2586
Size: 1881 5386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5458 5492 1903
Size: 34 3521 34 3678
Fragments arranged by size:
3678 3521 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5159 6369 5159
Size: 1210 6057
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7267
BfrBI ATG'CAT
Cuts at: 2509 2509
Size: 7267
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5878 136
Size: 107 122 71 631 605 4206 1525
Fragments arranged by size:
4206 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2343 7262 2343
Size: 4919 2348
BlpI GC'TnA_GC
Cuts at: 3506 4684 3506
Size: 1178 6089
Bme1580I G_kGCm'C
Cuts at: 1790 2367 2480 2520 2621 2870 3448 3567 4093
Size: 577 113 40 101 249 578 119 526
Cuts at: 4093 4359 4523 4545 5328 6484 1790
Size: 266 164 22 783 1156 2573
Fragments arranged by size:
2573 1156 783 578 577 526 266 249
164 119 113 101 40 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7267
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2726 4325 4998
Size: 416 53 83 399 24 1309 1599 673
Cuts at: 4998 5956 442
Size: 958 1753
Fragments arranged by size:
1753 1599 1309 958 673 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2754 2874 3117 3497 3689 4343 5927
Size: 812 703 120 243 380 192 654 1584
Cuts at: 5927 1239
Size: 2579
Fragments arranged by size:
2579 1584 812 703 654 380 243 192
120
Bpu10I CC'TnA_GC
Cuts at: 2586 4467 2586
Size: 1881 5386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2954 3368 5392 6257 6555 2954
Size: 414 2024 865 298 3666
Fragments arranged by size:
3666 2024 865 414 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5930 882
Size: 617 4431 2219
Fragments arranged by size:
4431 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5058 493
Size: 711 3854 2702
Fragments arranged by size:
3854 2702 711
BsaBI GATnn'nnATC
Cuts at: 7261 7261
Size: 7267
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2629 3751 5074 5456
Size: 53 83 186 936 1096 1122 1323 382
Cuts at: 5456 6091 275
Size: 635 1451
Fragments arranged by size:
1451 1323 1122 1096 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3038 5700 6098 6372 6519 6930 3038
Size: 2662 398 274 147 411 3375
Fragments arranged by size:
3375 2662 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2521 2551 3557 3587 3635
Size: 30 165 30 1118 30 1006 30 48
Cuts at: 3635 3665 4013 4043 1178
Size: 30 348 30 4402
Fragments arranged by size:
4402 1118 1006 348 165 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2535 2538 2547 3515 4264 4340 1186
Size: 1349 3 9 968 749 76 4113
Fragments arranged by size:
4113 1349 968 749 76 9 3
BseYI C'CCAG_C
Cuts at: 2519 2532 2687 3197 3356 3399 4346 4474 6470
Size: 13 155 510 159 43 947 128 1996
Cuts at: 6470 6966 2519
Size: 496 2820
Fragments arranged by size:
2820 1996 947 510 496 159 155 128
43 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2510 4087 1163
Size: 573 678 96 1577 4343
Fragments arranged by size:
4343 1577 678 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4004 4717 5478 6506 1540
Size: 275 186 2003 713 761 1028 2301
Fragments arranged by size:
2301 2003 1028 761 713 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 2978 3483 3567 3675 5328 5413 6484 2427
Size: 551 505 84 108 1653 85 1071 3210
Fragments arranged by size:
3210 1653 1071 551 505 108 85 84
BsmI GAATG_Cn'
Cuts at: 2245 2509 4778 4871 2245
Size: 264 2269 93 4641
Fragments arranged by size:
4641 2269 264 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4205 4205
Size: 7267
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2427 2480 2520 2621 2870 2978
Size: 399 577 60 53 40 101 249 108
Cuts at: 2978 3448 3483 3567 3675 3942 4093 4359 4481
Size: 470 35 84 108 267 151 266 122
Cuts at: 4481 4523 4545 4586 5328 5413 6484 1391
Size: 42 22 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 470 399 267 266
249 151 122 108 108 101 85 84
60 53 42 41 40 35 22
BspEI T'CCGG_A
Cuts at: 6930 6930
Size: 7267
BspHI T'CATG_A
Cuts at: 5156 6886 5156
Size: 1730 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3021 3304 3406 3687 3753 3771 4233 4622
Size: 2177 283 102 281 66 18 462 389
Cuts at: 4622 844
Size: 3489
Fragments arranged by size:
3489 2177 462 389 283 281 102 66
18
BsrBI CCG'CTC
Cuts at: 1394 1823 5154 1394
Size: 429 3331 3507
Fragments arranged by size:
3507 3331 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3060 5762 5936 65
Size: 1183 1812 2702 174 1396
Fragments arranged by size:
2702 1812 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3038 3999 5911 6098 1700
Size: 110 1228 961 1912 187 2869
Fragments arranged by size:
2869 1912 1228 961 187 110
BsrGI T'GTAC_A
Cuts at: 96 2664 2793 3420 96
Size: 2568 129 627 3943
Fragments arranged by size:
3943 2568 627 129
BssHII G'CGCG_C
Cuts at: 1925 4111 1925
Size: 2186 5081
BssSI C'ACGA_G
Cuts at: 3935 5327 6339 3935
Size: 1392 1012 4863
Fragments arranged by size:
4863 1392 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 3679 794
Size: 1721 1164 4382
Fragments arranged by size:
4382 1721 1164
BstBI TT'CG_AA
Cuts at: 6071 6071
Size: 7267
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7267
BstXI CCAn_nnnn'nTGG
Cuts at: 3316 4417 3316
Size: 1101 6166
BstYI r'GATC_y
Cuts at: 1972 2343 2706 4597 4964 5349 5366 6818 6904
Size: 371 363 1891 367 385 17 1452 86
Cuts at: 6904 6916 7262 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1891 1452 385 371 367 363 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3660 3660
Size: 7267
BtgI C'CryG_G
Cuts at: 513 1065 2004 3766 513
Size: 552 939 1762 4014
Fragments arranged by size:
4014 1762 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2430 487
Size: 683 56 1204 5324
Fragments arranged by size:
5324 1204 683 56
BtsI GCAGTG_nn'
Cuts at: 2564 3712 4282 4783 5577 5597 2564
Size: 1148 570 501 794 20 4234
Fragments arranged by size:
4234 1148 794 570 501 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2623 2658 529
Size: 35 2059 35 5138
Fragments arranged by size:
5138 2059 35 35
DraI TTT'AAA
Cuts at: 4571 4571
Size: 7267
DraIII CAC_nnn'GTG
Cuts at: 3124 3124
Size: 7267
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3617 6274 809
Size: 507 2301 2657 1802
Fragments arranged by size:
2657 2301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2253 2268
Size: 54 1425 50 275 18 250 173 15
Cuts at: 2268 2874 3070 4001 4714 5603 7232 8
Size: 606 196 931 713 889 1629 43
Fragments arranged by size:
1629 1425 931 889 713 606 275 250
196 173 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4001 4714 1537
Size: 275 2189 713 4090
Fragments arranged by size:
4090 2189 713 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3282 3911 5197 1353
Size: 1929 629 1286 3423
Fragments arranged by size:
3423 1929 1286 629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4511 5854 6228 6374 387
Size: 1064 138 2922 1343 374 146 1280
Fragments arranged by size:
2922 1343 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2754 2874 2921 3117 3497 3689
Size: 393 419 703 120 47 196 380 192
Cuts at: 3689 3935 4034 4063 4343 4352 5330 5927 6714
Size: 246 99 29 280 9 978 597 787
Cuts at: 6714 6982 1239
Size: 268 1524
Fragments arranged by size:
1524 978 787 703 597 419 393 380
280 268 246 196 192 120 99 47
29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2393
Size: 7267
EcoO109I rG'GnC_Cy
Cuts at: 2026 2320 2476 2477 2618 3664 4222 4519 2026
Size: 294 156 1 141 1046 558 297 4774
Fragments arranged by size:
4774 1046 558 297 294 156 141 1
EcoRI G'AATT_C
Cuts at: 4576 4954 4576
Size: 378 6889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3826 4399 3826
Size: 573 6694
FspI TGC'GCA
Cuts at: 5773 5773
Size: 7267
HaeII r_GCGC'y
Cuts at: 1678 2859 6414 1678
Size: 1181 3555 2531
Fragments arranged by size:
3555 2531 1181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3527 3559 3676 3708 5908
Size: 32 135 32 1955 32 117 32 2200
Cuts at: 5908 5940 5982 6014 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2200 1955 135 117 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3643 4611 4857 669
Size: 988 281 1705 968 246 3079
Fragments arranged by size:
3079 1705 988 968 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7267
HpaI GTT'AAC
Cuts at: 4857 4857
Size: 7267
KpnI G_GTAC'C
Cuts at: 4457 4592 4457
Size: 135 7132
MfeI C'AATT_G
Cuts at: 4866 4866
Size: 7267
MluI A'CGCG_T
Cuts at: 7143 7143
Size: 7267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2126 4506 6381 6565 1305
Size: 821 2380 1875 184 2007
Fragments arranged by size:
2380 2007 1875 821 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2082 2255 2270 2876 10
Size: 54 1425 343 250 173 15 606 4401
Fragments arranged by size:
4401 1425 606 343 250 173 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2408 3039 3144 3408 3855 4415
Size: 648 1194 48 631 105 264 447 560
Cuts at: 4415 5225 5584 5743 518
Size: 810 359 159 2042
Fragments arranged by size:
2042 1194 810 648 631 560 447 359
264 159 105 48
MspA1I CmG'CkG
Cuts at: 1606 2309 3278 3516 3774 4140 4194 4683 5360
Size: 703 969 238 258 366 54 489 677
Cuts at: 5360 6508 6753 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 969 703 677 489 366 258
245 238 54
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5776
Fragments arranged by size:
5776 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7267
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7267
NotI GC'GGCC_GC
Cuts at: 4714 4714
Size: 7267
NsiI A_TGCA'T
Cuts at: 2511 2511
Size: 7267
NspI r_CATG'y
Cuts at: 1132 1229 2509 3069 3112 3842 3928 4625 6170
Size: 97 1280 560 43 730 86 697 1545
Cuts at: 6170 1132
Size: 2229
Fragments arranged by size:
2229 1545 1280 730 697 560 97 86
43
PasI CC'CwG_GG
Cuts at: 1348 2473 2624 1348
Size: 1125 151 5991
Fragments arranged by size:
5991 1125 151
PciI A'CATG_T
Cuts at: 3924 6166 3924
Size: 2242 5025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 2389 2614 2716 2761 1108
Size: 1035 246 225 102 45 5614
Fragments arranged by size:
5614 1035 246 225 102 45
PfoI T'CCnGG_A
Cuts at: 2934 3133 2934
Size: 199 7068
PmeI GTTT'AAAC
Cuts at: 4571 4571
Size: 7267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5336 5368 6849 6881 3878
Size: 32 1426 32 1481 32 4264
Fragments arranged by size:
4264 1481 1426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4222 4222
Size: 7267
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7267
PsiI TTA'TAA
Cuts at: 4837 4837
Size: 7267
PspOMI G'GGCC_C
Cuts at: 2476 2617 4519 2476
Size: 141 1902 5224
Fragments arranged by size:
5224 1902 141
PstI C_TGCA'G
Cuts at: 830 2947 3018 3684 3986 4506 4619 830
Size: 2117 71 666 302 520 113 3478
Fragments arranged by size:
3478 2117 666 520 302 113 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7267
PvuII CAG'CTG
Cuts at: 3278 3278
Size: 7267
SalI G'TCGA_C
Cuts at: 1936 4609 1936
Size: 2673 4594
SbfI CC_TGCA'GG
Cuts at: 4619 4619
Size: 7267
ScaI AGT'ACT
Cuts at: 3572 5515 7227 3572
Size: 1943 1712 3612
Fragments arranged by size:
3612 1943 1712
SfcI C'TryA_G
Cuts at: 826 1045 2943 3014 3680 3982 4502 4615 5750
Size: 219 1898 71 666 302 520 113 1135
Cuts at: 5750 6431 6622 826
Size: 681 191 1471
Fragments arranged by size:
1898 1471 1135 681 666 520 302 219
191 113 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7267
SgrAI Cr'CCGG_yG
Cuts at: 1700 3038 1700
Size: 1338 5929
SmaI CCC'GGG
Cuts at: 1872 4594 1872
Size: 2722 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2969 3383 5371 6272 6534 820
Size: 197 926 1026 414 1988 901 262 1553
Fragments arranged by size:
1988 1553 1026 926 901 414 262 197
SnaBI TAC'GTA
Cuts at: 493 5058 493
Size: 4565 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7267
SphI G_CATG'C
Cuts at: 1132 3842 4625 1132
Size: 2710 783 3774
Fragments arranged by size:
3774 2710 783
SspI AAT'ATT
Cuts at: 5 52 5191 5
Size: 47 5139 2081
Fragments arranged by size:
5139 2081 47
StuI AGG'CCT
Cuts at: 1517 3266 4559 1517
Size: 1749 1293 4225
Fragments arranged by size:
4225 1749 1293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 4155 4706 513
Size: 552 939 79 2072 551 3074
Fragments arranged by size:
3074 2072 939 552 551 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2546 3779 4409 5294 5311 5464
Size: 1255 737 229 1233 630 885 17 153
Cuts at: 5464 5649 6094 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1233 885 737 630 445 229
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 2793 3420
Size: 274 80 33 51 1443 687 129 627
Cuts at: 3420 3570 5513 7225 96
Size: 150 1943 1712 138
Fragments arranged by size:
1943 1712 1443 687 627 274 150 138
129 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2521 2553 3557 3589 4013 4045 7227 7259 2521
Size: 32 1004 32 424 32 3182 32 2529
Fragments arranged by size:
3182 2529 1004 424 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7267
XbaI T'CTAG_A
Cuts at: 4603 4603
Size: 7267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 4489 2263
Size: 2226 5041
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7267
XmaI C'CCGG_G
Cuts at: 1870 4592 1870
Size: 2722 4545
XmnI GAAnn'nnTTC
Cuts at: 2186 3050 3809 5396 2186
Size: 864 759 1587 4057
Fragments arranged by size:
4057 1587 864 759
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5075 276
Size: 53 83 186 936 3541 2468
Fragments arranged by size:
3541 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI