Restriction map for
pFN21AB0423
(Circular) (Six-base) MAPSORT of: pFN21AB0423.seq Check: 4084 from: 1 to: 6127
pFN21AB0423 6127 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2140 3470 1937
Size: 203 1330 4594
Fragments arranged by size:
4594 1330 203
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 4190 5574 5842 1632
Size: 1379 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1379 1179 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2501 3870 5442 2501
Size: 1369 1572 3186
Fragments arranged by size:
3186 1572 1369
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2038 3436 3662 3814 5923 1493
Size: 545 1398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 545 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 3452 1870
Size: 73 361 1148 4545
Fragments arranged by size:
4545 1148 361 73
BamHI G'GATC_C
Cuts at: 2617 2789 3457 3824 2617
Size: 172 668 367 4920
Fragments arranged by size:
4920 668 367 172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 3448 4903 618
Size: 325 217 627 415 1246 1455 1842
Fragments arranged by size:
1842 1455 1246 627 415 325 217
BanII G_rGCy'C
Cuts at: 1391 3249 3446 1391
Size: 1858 197 4072
Fragments arranged by size:
4072 1858 197
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2448 3272 928
Size: 971 549 824 3783
Fragments arranged by size:
3783 971 824 549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2756 2756
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 4188 5344 1790
Size: 2398 1156 2573
Fragments arranged by size:
2573 2398 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2941 3163 2911
Size: 30 222 5875
Fragments arranged by size:
5875 222 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2565 2881 4787 1239
Size: 1326 316 1906 2579
Fragments arranged by size:
2579 1906 1326 316
Bpu10I CC'TnA_GC
Cuts at: 2724 2724
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3000 4560 4958 5232 5379 5790 3000
Size: 1560 398 274 147 411 3337
Fragments arranged by size:
3337 1560 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2768 2798 2893
Size: 30 165 30 792 30 543 30 95
Cuts at: 2893 2923 1178
Size: 30 4382
Fragments arranged by size:
4382 792 543 165 95 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2124 2486 2917 1186
Size: 938 362 431 4396
Fragments arranged by size:
4396 938 431 362
BseYI C'CCAG_C
Cuts at: 5330 5826 5330
Size: 496 5631
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2886 1163
Size: 573 1150 4404
Fragments arranged by size:
4404 1150 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2781 3171 3249 4188 4273 5344 2100
Size: 681 390 78 939 85 1071 2883
Fragments arranged by size:
2883 1071 939 681 390 85 78
BsmI GAATG_Cn'
Cuts at: 2642 3638 3731 2642
Size: 996 93 5038
Fragments arranged by size:
5038 996 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2781 3171 3249 3446 4188 4273
Size: 399 310 681 390 78 197 742 85
Cuts at: 4273 5344 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 681 399 390 310 197
85 78
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 2311 4016 5746 2311
Size: 1705 1730 2692
Fragments arranged by size:
2692 1730 1705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6127
BstBI TT'CG_AA
Cuts at: 2082 4931 2082
Size: 2849 3278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2289 2333 2366 2672 2848 2289
Size: 44 33 306 176 5568
Fragments arranged by size:
5568 306 176 44 33
BstYI r'GATC_y
Cuts at: 1972 2617 2657 2789 3457 3824 4209 4226 5678
Size: 645 40 132 668 367 385 17 1452
Cuts at: 5678 5764 5776 6122 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 668 645 385 367 346 132
86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2691 513
Size: 552 939 687 3949
Fragments arranged by size:
3949 939 687 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2339 487
Size: 683 56 1113 4275
Fragments arranged by size:
4275 1113 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 3643 4437 4457 3403
Size: 240 794 20 5073
Fragments arranged by size:
5073 794 240 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2243 5134 809
Size: 507 927 2891 1802
Fragments arranged by size:
2891 1802 927 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092
Size: 54 1425 50 275 18 1744 889 1629
Cuts at: 6092 8
Size: 43
Fragments arranged by size:
1744 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2305 2463 2963 4057 1353
Size: 952 158 500 1094 3423
Fragments arranged by size:
3423 1094 952 500 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 4714 5088 5234 387
Size: 1064 138 621 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 621 374 146 138
EcoICRI GAG'CTC
Cuts at: 3247 3247
Size: 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2565 2881 3011 4190 4787 5574 5842
Size: 393 933 316 130 1179 597 787 268
Cuts at: 5842 1239
Size: 1524
Fragments arranged by size:
1524 1179 933 787 597 393 316 268
130
EcoNI CCTnn'n_nnAGG
Cuts at: 3236 3236
Size: 6127
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3322 3322
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 5274 1678
Size: 3596 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2768 2800 4768 4800 4842
Size: 32 135 32 1196 32 1968 32 42
Cuts at: 4842 4874 1373
Size: 32 2626
Fragments arranged by size:
2626 1968 1196 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2141 2946 3471 3717 669
Size: 988 281 203 805 525 246 3079
Fragments arranged by size:
3079 988 805 525 281 246 203
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 2913 3726 2913
Size: 813 5314
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5241 5425 1305
Size: 3936 184 2007
Fragments arranged by size:
3936 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 2670 3105 4085 4444 4603 518
Size: 648 1198 306 435 980 359 159 2042
Fragments arranged by size:
2042 1198 980 648 435 359 306 159
MspA1I CmG'CkG
Cuts at: 1606 3277 3543 4220 5368 5613 1606
Size: 1671 266 677 1148 245 2120
Fragments arranged by size:
2120 1671 1148 677 266 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4636
Fragments arranged by size:
4636 939 552
NdeI CA'TA_TG
Cuts at: 387 2260 387
Size: 1873 4254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2758 2758
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 3485 5030 1132
Size: 97 2256 1545 2229
Fragments arranged by size:
2256 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2501 1108
Size: 1393 4734
PfoI T'CCnGG_A
Cuts at: 2381 2381
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3339 4196 4228 5709 5741 3307
Size: 32 857 32 1481 32 3693
Fragments arranged by size:
3693 1481 857 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PstI C_TGCA'G
Cuts at: 830 2191 3479 830
Size: 1361 1288 3478
Fragments arranged by size:
3478 1361 1288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 3277 3277
Size: 6127
SacI G_AGCT'C
Cuts at: 3249 3249
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 2139 3469 1936
Size: 203 1330 4594
Fragments arranged by size:
4594 1330 203
SapI GCTCTTCn'nnn_
Cuts at: 2305 2305
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SexAI A'CCwGG_T
Cuts at: 2715 2715
Size: 6127
SfcI C'TryA_G
Cuts at: 826 1045 2187 3123 3475 4610 5291 5482 826
Size: 219 1142 936 352 1135 681 191 1471
Fragments arranged by size:
1471 1142 1135 936 681 352 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4231 5132 5394 820
Size: 197 926 2288 901 262 1553
Fragments arranged by size:
2288 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 3341 3566 513
Size: 552 939 1337 225 3074
Fragments arranged by size:
3074 1337 939 552 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4373 6085 96
Size: 274 80 33 51 1443 2396 1712 138
Fragments arranged by size:
2396 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 2377 2409 2893 2925 6087 6119 2195
Size: 32 150 32 484 32 3162 32 2203
Fragments arranged by size:
3162 2203 484 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3157 3463 3157
Size: 306 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 3305 4256 3305
Size: 951 5176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI