Restriction map for
pFN21AB0420
(Circular) (Six-base) MAPSORT of: pFN21AB0420.seq Check: 4452 from: 1 to: 7144
pFN21AB0420 7144 bp
With 182 enzymes: SgfI *
March 28, 2012 12:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2182 2182
Size: 7144
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4954 278
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4014 4487 1937
Size: 2077 473 4594
Fragments arranged by size:
4594 2077 473
Acc65I G'GTAC_C
Cuts at: 4465 4465
Size: 7144
AclI AA'CG_TT
Cuts at: 1563 5271 5644 1563
Size: 3708 373 3063
Fragments arranged by size:
3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 2382 2565 5207 6591 6859 1632
Size: 521 229 183 2642 1384 268 1917
Fragments arranged by size:
2642 1917 1384 521 268 229 183
AflII C'TTAA_G
Cuts at: 820 1017 2708 820
Size: 197 1691 5256
Fragments arranged by size:
5256 1691 197
AflIII A'CryG_T
Cuts at: 1203 6043 7020 1203
Size: 4840 977 1327
Fragments arranged by size:
4840 1327 977
AgeI A'CCGG_T
Cuts at: 2227 5975 2227
Size: 3748 3396
AhdI GACnn_n'nnGTC
Cuts at: 5873 5873
Size: 7144
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7144
AlwNI CAG_nnn'CTG
Cuts at: 3787 4183 4887 6459 3787
Size: 396 704 1572 4472
Fragments arranged by size:
4472 1572 704 396
ApaLI G'TGCA_C
Cuts at: 3900 4134 5201 6357 3900
Size: 234 1067 1156 4687
Fragments arranged by size:
4687 1156 1067 234
ApoI r'AATT_y
Cuts at: 1493 2282 3819 4453 4679 4831 6940 1493
Size: 789 1537 634 226 152 2109 1697
Fragments arranged by size:
2109 1697 1537 789 634 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5698 160
Size: 872 4666 1606
Fragments arranged by size:
4666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
AvaI C'yCGr_G
Cuts at: 1870 1943 4469 1870
Size: 73 2526 4545
Fragments arranged by size:
4545 2526 73
AvrII C'CTAG_G
Cuts at: 4268 4268
Size: 7144
BamHI G'GATC_C
Cuts at: 4474 4841 4474
Size: 367 6777
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4465 5920 618
Size: 325 217 627 2678 1455 1842
Fragments arranged by size:
2678 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3415 4463 1391
Size: 2024 1048 4072
Fragments arranged by size:
4072 2024 1048
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3085 3192 3379 928
Size: 971 1186 107 187 4693
Fragments arranged by size:
4693 1186 971 187 107
BbvCI CC'TCA_GC
Cuts at: 3180 3180
Size: 7144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 5335 5369 1903
Size: 34 98 34 3266 34 3678
Fragments arranged by size:
3678 3266 98 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5036 6246 5036
Size: 1210 5934
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5755 136
Size: 107 122 71 631 605 4083 1525
Fragments arranged by size:
4083 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2722 3106 7139 2722
Size: 384 4033 2727
Fragments arranged by size:
4033 2727 384
BlpI GC'TnA_GC
Cuts at: 4561 4561
Size: 7144
Bme1580I G_kGCm'C
Cuts at: 1790 3904 4138 5205 6361 1790
Size: 2114 234 1067 1156 2573
Fragments arranged by size:
2573 2114 1156 1067 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7144
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4875 5833 442
Size: 416 53 83 399 24 3458 958 1753
Fragments arranged by size:
3458 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3465 5804 1239
Size: 2226 2339 2579
Fragments arranged by size:
2579 2339 2226
Bpu10I CC'TnA_GC
Cuts at: 3180 3180
Size: 7144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5269 6134 6432 5269
Size: 865 298 5981
Fragments arranged by size:
5981 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5807 882
Size: 617 4308 2219
Fragments arranged by size:
4308 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4935 493
Size: 711 3731 2702
Fragments arranged by size:
3731 2702 711
BsaBI GATnn'nnATC
Cuts at: 3111 7138 3111
Size: 4027 3117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3993 4951 5333
Size: 53 83 186 936 507 1953 958 382
Cuts at: 5333 5968 275
Size: 635 1451
Fragments arranged by size:
1953 1451 958 936 635 507 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2132 2227 5577 5975 6249 6396 6807 2132
Size: 95 3350 398 274 147 411 2469
Fragments arranged by size:
3350 2469 411 398 274 147 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6919
Fragments arranged by size:
6919 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2438 3237 1186
Size: 979 273 799 5093
Fragments arranged by size:
5093 979 799 273
BseYI C'CCAG_C
Cuts at: 3128 6347 6843 3128
Size: 3219 496 3429
Fragments arranged by size:
3429 3219 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 3066 1163
Size: 573 336 994 5241
Fragments arranged by size:
5241 994 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3005 4594 5355 6383 1540
Size: 275 186 1004 1589 761 1028 2301
Fragments arranged by size:
2301 1589 1028 1004 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3415 3904 4138 5205 5290 6361 3415
Size: 489 234 1067 85 1071 4198
Fragments arranged by size:
4198 1071 1067 489 234 85
BsmI GAATG_Cn'
Cuts at: 4655 4748 4655
Size: 93 7051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3415 3904 4138 4463 5205 5290 6361
Size: 399 1625 489 234 325 742 85 1071
Cuts at: 6361 1391
Size: 2174
Fragments arranged by size:
2174 1625 1071 742 489 399 325 234
85
BspEI T'CCGG_A
Cuts at: 6807 6807
Size: 7144
BspHI T'CATG_A
Cuts at: 4381 5033 6763 4381
Size: 652 1730 4762
Fragments arranged by size:
4762 1730 652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 2182 4070 4499 844
Size: 1250 88 1888 429 3489
Fragments arranged by size:
3489 1888 1250 429 88
BsrBI CCG'CTC
Cuts at: 1394 1823 5031 1394
Size: 429 3208 3507
Fragments arranged by size:
3507 3208 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2411 2955 5639 5813 65
Size: 1183 1163 544 2684 174 1396
Fragments arranged by size:
2684 1396 1183 1163 544 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2056 2227 5788 5975 1700
Size: 110 246 171 3561 187 2869
Fragments arranged by size:
3561 2869 246 187 171 110
BsrGI T'GTAC_A
Cuts at: 96 4057 96
Size: 3961 3183
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7144
BssSI C'ACGA_G
Cuts at: 5204 6216 5204
Size: 1012 6132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3746 3908 794
Size: 2952 162 4030
Fragments arranged by size:
4030 2952 162
BstBI TT'CG_AA
Cuts at: 5948 5948
Size: 7144
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7144
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2137
Size: 7144
BstYI r'GATC_y
Cuts at: 1972 2074 2722 3106 4474 4841 5226 5243 6695
Size: 102 648 384 1368 367 385 17 1452
Cuts at: 6695 6781 6793 7139 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1368 648 385 384 367 346
102 86 17 12
BstZ17I GTA'TAC
Cuts at: 4015 4015
Size: 7144
Bsu36I CC'TnA_GG
Cuts at: 4006 4006
Size: 7144
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6592
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4445 487
Size: 683 56 3219 3186
Fragments arranged by size:
3219 3186 683 56
BtsI GCAGTG_nn'
Cuts at: 2458 3083 3736 4660 5454 5474 2458
Size: 625 653 924 794 20 4128
Fragments arranged by size:
4128 924 794 653 625 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7109
DraI TTT'AAA
Cuts at: 2935 4448 2935
Size: 1513 5631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6151 809
Size: 507 4835 1802
Fragments arranged by size:
4835 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4308 4591 5480
Size: 54 1425 50 275 18 2478 283 889
Cuts at: 5480 7109 8
Size: 1629 43
Fragments arranged by size:
2478 1629 1425 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4591 1537
Size: 275 2779 4090
Fragments arranged by size:
4090 2779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2201 2758 3175 5074 1353
Size: 789 59 557 417 1899 3423
Fragments arranged by size:
3423 1899 789 557 417 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5731 6105 6251 387
Size: 1064 138 4142 374 146 1280
Fragments arranged by size:
4142 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3413 3413
Size: 7144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2382 2565 3465 5207 5804 6591
Size: 393 521 229 183 900 1742 597 787
Cuts at: 6591 6859 1239
Size: 268 1524
Fragments arranged by size:
1742 1524 900 787 597 521 393 268
229 183
EcoO109I rG'GnC_Cy
Cuts at: 3690 4339 3690
Size: 649 6495
EcoRI G'AATT_C
Cuts at: 4453 4831 4453
Size: 378 6766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3011 3011
Size: 7144
FspI TGC'GCA
Cuts at: 5650 5650
Size: 7144
HaeII r_GCGC'y
Cuts at: 1678 2036 6291 1678
Size: 358 4255 2531
Fragments arranged by size:
4255 2531 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 2837 2869 3620
Size: 32 135 32 1103 32 130 32 751
Cuts at: 3620 3652 5785 5817 5859 5891 1373
Size: 32 2133 32 42 32 2626
Fragments arranged by size:
2626 2133 1103 751 135 130 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4488 4734 669
Size: 988 281 2550 246 3079
Fragments arranged by size:
3079 2550 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7144
HpaI GTT'AAC
Cuts at: 4734 4734
Size: 7144
KpnI G_GTAC'C
Cuts at: 4469 4469
Size: 7144
MfeI C'AATT_G
Cuts at: 4743 4743
Size: 7144
MluI A'CGCG_T
Cuts at: 7020 7020
Size: 7144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 3025 6258 6442 1305
Size: 831 889 3233 184 2007
Fragments arranged by size:
3233 2007 889 831 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5322
Fragments arranged by size:
5322 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3577 3618 5102 5461 5620 518
Size: 648 969 1442 41 1484 359 159 2042
Fragments arranged by size:
2042 1484 1442 969 648 359 159 41
MspA1I CmG'CkG
Cuts at: 1606 2053 2067 2173 3053 4313 4560 5237 6385
Size: 447 14 106 880 1260 247 677 1148
Cuts at: 6385 6630 1606
Size: 245 2120
Fragments arranged by size:
2120 1260 1148 880 677 447 247 245
106 14
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6592
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7144
NotI GC'GGCC_GC
Cuts at: 4591 4591
Size: 7144
NspI r_CATG'y
Cuts at: 1132 1229 3702 4502 6047 1132
Size: 97 2473 800 1545 2229
Fragments arranged by size:
2473 2229 1545 800 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7144
PciI A'CATG_T
Cuts at: 6043 6043
Size: 7144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7144
PmeI GTTT'AAAC
Cuts at: 4448 4448
Size: 7144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5213 5245 6726 6758 5213
Size: 32 1481 32 5599
Fragments arranged by size:
5599 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3690 4339 3690
Size: 649 6495
PshAI GACnn'nnGTC
Cuts at: 1613 3546 1613
Size: 1933 5211
PsiI TTA'TAA
Cuts at: 4714 4714
Size: 7144
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3718 3750 4350 4382 3718
Size: 32 600 32 6480
Fragments arranged by size:
6480 600 32 32
PstI C_TGCA'G
Cuts at: 830 2478 4318 4496 830
Size: 1648 1840 178 3478
Fragments arranged by size:
3478 1840 1648 178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7144
SacI G_AGCT'C
Cuts at: 3415 3415
Size: 7144
SalI G'TCGA_C
Cuts at: 1936 4486 1936
Size: 2550 4594
SanDI GG'GwC_CC
Cuts at: 3690 3690
Size: 7144
SapI GCTCTTCn'nnn_
Cuts at: 3175 3175
Size: 7144
SbfI CC_TGCA'GG
Cuts at: 4496 4496
Size: 7144
ScaI AGT'ACT
Cuts at: 3239 4039 5392 7104 3239
Size: 800 1353 1712 3279
Fragments arranged by size:
3279 1712 1353 800
SfcI C'TryA_G
Cuts at: 826 1045 2474 3640 4314 4356 4492 5627 6308
Size: 219 1429 1166 674 42 136 1135 681
Cuts at: 6308 6499 826
Size: 191 1471
Fragments arranged by size:
1471 1429 1166 1135 681 674 219 191
136 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7144
SmaI CCC'GGG
Cuts at: 1872 4471 1872
Size: 2599 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2708 5248 6149 6411 820
Size: 197 926 765 2540 901 262 1553
Fragments arranged by size:
2540 1553 926 901 765 262 197
SnaBI TAC'GTA
Cuts at: 493 4935 493
Size: 4442 2702
SpeI A'CTAG_T
Cuts at: 152 3281 3556 152
Size: 3129 275 3740
Fragments arranged by size:
3740 3129 275
SphI G_CATG'C
Cuts at: 1132 4502 1132
Size: 3370 3774
SspI AAT'ATT
Cuts at: 5 52 2251 2534 4108 5068 5
Size: 47 2199 283 1574 960 2081
Fragments arranged by size:
2199 2081 1574 960 283 47
StuI AGG'CCT
Cuts at: 1517 3595 1517
Size: 2078 5066
StyI C'CwwG_G
Cuts at: 513 1065 3275 4268 4335 4370 4583 513
Size: 552 2210 993 67 35 213 3074
Fragments arranged by size:
3074 2210 993 552 213 67 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3019 5171 5188 5341 5526 5971 325
Size: 1255 1439 2152 17 153 185 445 1498
Fragments arranged by size:
2152 1498 1439 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3237 4037 4057
Size: 274 80 33 51 1443 1260 800 20
Cuts at: 4057 5390 7102 96
Size: 1333 1712 138
Fragments arranged by size:
1712 1443 1333 1260 800 274 138 80
51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2605 2637 3969 4001 4264 4296 7104 7136 2605
Size: 32 1332 32 263 32 2808 32 2613
Fragments arranged by size:
2808 2613 1332 263 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7144
XbaI T'CTAG_A
Cuts at: 2746 4480 2746
Size: 1734 5410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3161 3356 3161
Size: 195 6949
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7144
XmaI C'CCGG_G
Cuts at: 1870 4469 1870
Size: 2599 4545
XmnI GAAnn'nnTTC
Cuts at: 2963 5273 2963
Size: 2310 4834
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4952 276
Size: 53 83 186 936 3418 2468
Fragments arranged by size:
3418 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PvuII RsrII SacII SexAI SfiI SfoI
SrfI SwaI