Restriction map for
pFN21AB0417
(Circular) (Six-base) MAPSORT of: pFN21AB0417.seq Check: 2744 from: 1 to: 6118
pFN21AB0417 6118 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2336 3303 4181 5565 5833 1632
Size: 704 967 878 1384 268 1917
Fragments arranged by size:
1917 1384 967 878 704 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 3148 3861 5433 3148
Size: 713 1572 3833
Fragments arranged by size:
3833 1572 713
ApaI G_GGCC'C
Cuts at: 3355 3355
Size: 6118
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2011 2771 3427 3653 3805 5914 1493
Size: 518 760 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 760 656 518 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2867 2946 3443 1870
Size: 73 924 79 497 4545
Fragments arranged by size:
4545 924 497 79 73
BamHI G'GATC_C
Cuts at: 2612 3448 3815 2612
Size: 836 367 4915
Fragments arranged by size:
4915 836 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 3439 4894 618
Size: 325 217 627 304 1348 1455 1842
Fragments arranged by size:
1842 1455 1348 627 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2340 2680 3355 3437 1391
Size: 949 340 675 82 4072
Fragments arranged by size:
4072 949 675 340 82
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 6118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2439 3315 928
Size: 971 540 876 3731
Fragments arranged by size:
3731 971 876 540
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4010 5220 4010
Size: 1210 4908
BclI T'GATC_A
Cuts at: 1548 2118 1548
Size: 570 5548
BfrBI ATG'CAT
Cuts at: 2556 2556
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2407 3355 4179 5335 1790
Size: 376 241 948 824 1156 2573
Fragments arranged by size:
2573 1156 948 824 376 241
BmgBI CAC'GTC
Cuts at: 1318 2305 1318
Size: 987 5131
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3311 3849 4807
Size: 416 53 83 399 24 1894 538 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1894 1753 958 538 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2150 2231 2664 2693 3246 4778 1239
Size: 884 27 81 433 29 553 1532 2579
Fragments arranged by size:
2579 1532 884 553 433 81 29 27
Bpu10I CC'TnA_GC
Cuts at: 2630 2630
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3009 4243 5108 5406 3009
Size: 1234 865 298 3721
Fragments arranged by size:
3721 1234 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2092 3925 4307 4942
Size: 53 83 186 936 559 1833 382 635
Cuts at: 4942 275
Size: 1451
Fragments arranged by size:
1833 1451 936 635 559 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2812 2842 2970 3000 1178
Size: 30 165 30 1409 30 128 30 4296
Fragments arranged by size:
4296 1409 165 128 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2170 2228 1186
Size: 984 58 5076
Fragments arranged by size:
5076 984 58
BseYI C'CCAG_C
Cuts at: 5321 5817 5321
Size: 496 5622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5545
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2680 4179 4264 5335 2340
Size: 340 1499 85 1071 3123
Fragments arranged by size:
3123 1499 1071 340 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
BsmBI CGTCTCn'nnnn_
Cuts at: 2485 2485
Size: 6118
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2166 2340 2407 2680 3355 3437 4179
Size: 399 376 174 67 273 675 82 742
Cuts at: 4179 4264 5335 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 399 376 273 174
85 82 67
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 4007 5737 4007
Size: 1730 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 3473 844
Size: 1409 1220 3489
Fragments arranged by size:
3489 1409 1220
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 4613 4787 65
Size: 1183 1473 1892 174 1396
Fragments arranged by size:
1892 1473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2958 4762 4949 1700
Size: 110 278 870 1804 187 2869
Fragments arranged by size:
2869 1804 870 278 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 4544
BstBI TT'CG_AA
Cuts at: 2775 4922 2775
Size: 2147 3971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstXI CCAn_nnnn'nTGG
Cuts at: 2851 3085 2851
Size: 234 5884
BstYI r'GATC_y
Cuts at: 1972 2249 2565 2612 3261 3448 3815 4200 4217
Size: 277 316 47 649 187 367 385 17
Cuts at: 4217 5669 5755 5767 6113 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 649 385 367 346 316 277
187 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 3141 2876
Size: 265 5853
BtgI C'CryG_G
Cuts at: 513 1065 2004 2618 2989 513
Size: 552 939 614 371 3642
Fragments arranged by size:
3642 939 614 552 371
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 3634 4428 4448 3634
Size: 794 20 5304
Fragments arranged by size:
5304 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DraIII CAC_nnn'GTG
Cuts at: 3305 3305
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2685 3565
Size: 54 1425 50 275 18 205 650 880
Cuts at: 3565 4454 6083 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 880 650 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2300 2455 4048 1353
Size: 702 245 155 1593 3423
Fragments arranged by size:
3423 1593 702 245 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2820 4705 5079 5225 387
Size: 1064 138 1231 1885 374 146 1280
Fragments arranged by size:
1885 1280 1231 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2338 2678 2338
Size: 340 5778
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2150 2231 2336 2664 2693 3246
Size: 393 491 27 81 105 328 29 553
Cuts at: 3246 3303 4181 4778 5565 5833 1239
Size: 57 878 597 787 268 1524
Fragments arranged by size:
1524 878 787 597 553 491 393 328
268 105 81 57 29 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2213 2036
Size: 177 5941
EcoO109I rG'GnC_Cy
Cuts at: 2871 2871
Size: 6118
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
EcoRV GAT'ATC
Cuts at: 3232 3232
Size: 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3059 3059
Size: 6118
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2095 5265 1678
Size: 417 3170 2531
Fragments arranged by size:
3170 2531 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2110 2142 2241 2273 2487
Size: 32 135 32 538 32 99 32 214
Cuts at: 2487 2519 2818 2850 3164 3196 4759 4791 4833
Size: 32 299 32 314 32 1563 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 1563 538 314 299 214 135 99
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2178 3462 3708 669
Size: 988 281 240 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246 240
HindIII A'AGCT_T
Cuts at: 748 2481 3041 748
Size: 1733 560 3825
Fragments arranged by size:
3825 1733 560
HpaI GTT'AAC
Cuts at: 2178 3708 2178
Size: 1530 4588
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2868 5232 5416 1305
Size: 1563 2364 184 2007
Fragments arranged by size:
2364 2007 1563 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2037 2687 10
Size: 54 1425 343 205 650 3441
Fragments arranged by size:
3441 1425 650 343 205 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2551 2849 4076 4435 4594 518
Size: 648 1020 365 298 1227 359 159 2042
Fragments arranged by size:
2042 1227 1020 648 365 359 298 159
MspA1I CmG'CkG
Cuts at: 1606 3534 4211 5359 5604 1606
Size: 1928 677 1148 245 2120
Fragments arranged by size:
2120 1928 1148 677 245
NaeI GCC'GGC
Cuts at: 2090 2090
Size: 6118
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4627
Fragments arranged by size:
4627 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NgoMIV G'CCGG_C
Cuts at: 2088 2088
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NsiI A_TGCA'T
Cuts at: 2558 2558
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2371 2560 3476 5021 1132
Size: 97 1142 189 916 1545 2229
Fragments arranged by size:
2229 1545 1142 916 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PfoI T'CCnGG_A
Cuts at: 2139 2139
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2871 2871
Size: 6118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 2658 3688 2658
Size: 1030 5088
PspOMI G'GGCC_C
Cuts at: 3351 3351
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2264 3470 830
Size: 1434 1206 3478
Fragments arranged by size:
3478 1434 1206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
SacI G_AGCT'C
Cuts at: 2340 2680 2340
Size: 340 5778
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SanDI GG'GwC_CC
Cuts at: 2871 2871
Size: 6118
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 2858 4366 6078 2858
Size: 1508 1712 2898
Fragments arranged by size:
2898 1712 1508
SfcI C'TryA_G
Cuts at: 826 1045 2260 3123 3466 4601 5282 5473 826
Size: 219 1215 863 343 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 863 681 343 219 191
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 6118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3024 4222 5123 5385 820
Size: 197 926 1081 1198 901 262 1553
Fragments arranged by size:
1553 1198 1081 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 2371 2560 3476 1132
Size: 1239 189 916 3774
Fragments arranged by size:
3774 1239 916 189
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 2952 1517
Size: 1435 4683
StyI C'CwwG_G
Cuts at: 513 1065 2004 2032 2146 2389 3557 513
Size: 552 939 28 114 243 1168 3074
Fragments arranged by size:
3074 1168 939 552 243 114 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 3293 4145 4162 4315 4500 4945
Size: 1255 815 898 852 17 153 185 445
Cuts at: 4945 325
Size: 1498
Fragments arranged by size:
1498 1255 898 852 815 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2856 4364 6076
Size: 274 80 33 51 1443 879 1508 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
1712 1508 1443 879 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2305 2337 2970 3002 6078 6110 2305
Size: 32 633 32 3076 32 2313
Fragments arranged by size:
3076 2313 633 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3113 1316
Size: 1797 4321
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 2195 2771 4247 2195
Size: 576 1476 4066
Fragments arranged by size:
4066 1476 576
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
BsiWI BstZ17I FseI FspAI NruI PacI PmlI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI
SwaI XcmI