Restriction map for
pFN21AB0411
(Circular) (Six-base) MAPSORT of: pFN21AB0411.seq Check: 4963 from: 1 to: 6106
pFN21AB0411 6106 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2460 3449 1937
Size: 523 989 4594
Fragments arranged by size:
4594 989 523
Acc65I G'GTAC_C
Cuts at: 2212 3427 2212
Size: 1215 4891
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4169 5553 5821 1632
Size: 2537 1384 268 1917
Fragments arranged by size:
2537 1917 1384 268
AfeI AGC'GCT
Cuts at: 2993 2993
Size: 6106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 2151 4835 2151
Size: 2684 3422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3088 3251 1184
Size: 1904 163 4039
Fragments arranged by size:
4039 1904 163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 2631 2663 2407
Size: 32 192 32 5850
Fragments arranged by size:
5850 192 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3205 3849 5421 3205
Size: 644 1572 3890
Fragments arranged by size:
3890 1572 644
ApaLI G'TGCA_C
Cuts at: 3286 4163 5319 3286
Size: 877 1156 4073
Fragments arranged by size:
4073 1156 877
ApoI r'AATT_y
Cuts at: 1493 2325 2684 3415 3641 3793 5902 1493
Size: 832 359 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 832 731 359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2054 4660 160
Size: 872 1022 2606 1606
Fragments arranged by size:
2606 1606 1022 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2439 3431 1870
Size: 73 496 992 4545
Fragments arranged by size:
4545 992 496 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2204 2237 2204
Size: 33 6073
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2444 2972 3427 4882
Size: 325 217 627 425 232 528 455 1455
Cuts at: 4882 618
Size: 1842
Fragments arranged by size:
1842 1455 627 528 455 425 325 232
217
BanII G_rGCy'C
Cuts at: 1391 2891 3425 1391
Size: 1500 534 4072
Fragments arranged by size:
4072 1500 534
BbeI G_GCGC'C
Cuts at: 2448 2448
Size: 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 928
Size: 971 1164 3971
Fragments arranged by size:
3971 1164 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6101 6101
Size: 6106
BlpI GC'TnA_GC
Cuts at: 3004 3523 3004
Size: 519 5587
Bme1580I G_kGCm'C
Cuts at: 1790 2975 3290 4167 5323 1790
Size: 1185 315 877 1156 2573
Fragments arranged by size:
2573 1185 1156 877 315
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 2507 3837
Size: 416 53 83 399 24 713 377 1330
Cuts at: 3837 4795 442
Size: 958 1753
Fragments arranged by size:
1753 1330 958 713 416 399 377 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2672 2681 3131 4766 1239
Size: 1433 9 450 1635 2579
Fragments arranged by size:
2579 1635 1433 450 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 3302 4231 5096 5394 2702
Size: 600 929 865 298 3414
Fragments arranged by size:
3414 929 865 600 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 3913 4295 4930
Size: 53 83 186 936 912 1468 382 635
Cuts at: 4930 275
Size: 1451
Fragments arranged by size:
1468 1451 936 912 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2631 2661 1178
Size: 30 165 30 1228 30 4623
Fragments arranged by size:
4623 1228 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6106
BseYI C'CCAG_C
Cuts at: 5309 5805 5309
Size: 496 5610
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2926 1163
Size: 573 1190 4343
Fragments arranged by size:
4343 1190 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 3290 4167 4252 5323 2021
Size: 1269 877 85 1071 2804
Fragments arranged by size:
2804 1269 1071 877 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2891 2975 3290 3425 4167 4252
Size: 399 231 870 84 315 135 742 85
Cuts at: 4252 5323 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 870 742 399 315 231 135
85 84
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2758 3995 5725 2758
Size: 1237 1730 3139
Fragments arranged by size:
3139 1730 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3060 3270 4601 4775 65
Size: 1183 1812 210 1331 174 1396
Fragments arranged by size:
1812 1396 1331 1183 210 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3024 96
Size: 2928 3178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 2564 4166 5178 2564
Size: 1602 1012 3492
Fragments arranged by size:
3492 1602 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101
Size: 1464 367 385 17 1452 86 12 346
Cuts at: 6101 1972
Size: 1977
Fragments arranged by size:
1977 1464 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2461 2461
Size: 6106
Bsu36I CC'TnA_GG
Cuts at: 2856 2856
Size: 6106
BtgI C'CryG_G
Cuts at: 513 1065 2004 2216 513
Size: 552 939 212 4403
Fragments arranged by size:
4403 939 552 212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2557 487
Size: 683 56 1331 4036
Fragments arranged by size:
4036 1331 683 56
BtsI GCAGTG_nn'
Cuts at: 2283 3622 4416 4436 2283
Size: 1339 794 20 3953
Fragments arranged by size:
3953 1339 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 2067 3410 2067
Size: 1343 4763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2203 3553 4442
Size: 54 1425 50 275 18 373 1350 889
Cuts at: 4442 6071 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1350 889 373 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2109 4036 1353
Size: 681 75 1927 3423
Fragments arranged by size:
3423 1927 681 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4693 5067 5213 387
Size: 1064 138 3104 374 146 1280
Fragments arranged by size:
3104 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2672 2681 3131 4169 4766 5553 5821
Size: 393 1040 9 450 1038 597 787 268
Cuts at: 5821 1239
Size: 1524
Fragments arranged by size:
1524 1040 1038 787 597 450 393 268
9
EcoO109I rG'GnC_Cy
Cuts at: 2860 3163 2860
Size: 303 5803
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 6106
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2448 2995 3354 5253 1678
Size: 770 547 359 1899 2531
Fragments arranged by size:
2531 1899 770 547 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 2627 2659 4747
Size: 32 135 32 565 32 458 32 2088
Cuts at: 4747 4779 4821 4853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2088 565 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3450 3696 669
Size: 988 281 1512 246 3079
Fragments arranged by size:
3079 1512 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3102 748
Size: 2354 3752
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KasI G'GCGC_C
Cuts at: 2444 2444
Size: 6106
KpnI G_GTAC'C
Cuts at: 2216 3431 2216
Size: 1215 4891
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2304 5220 5404 1305
Size: 999 2916 184 2007
Fragments arranged by size:
2916 2007 999 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2205 10
Size: 54 1425 343 373 3911
Fragments arranged by size:
3911 1425 373 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2067 2298 2960 4064 4423 4582 518
Size: 648 901 231 662 1104 359 159 2042
Fragments arranged by size:
2042 1104 901 662 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 3522 4199 5347 5592 1606
Size: 1916 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 677 245
NarI GG'CG_CC
Cuts at: 2445 2445
Size: 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 2216 513
Size: 552 939 212 4403
Fragments arranged by size:
4403 939 552 212
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2714 2965 3464 5009 1132
Size: 97 1485 251 499 1545 2229
Fragments arranged by size:
2229 1545 1485 499 251 97
PacI TTA_AT'TAA
Cuts at: 2058 2058
Size: 6106
PasI CC'CwG_GG
Cuts at: 1348 2982 1348
Size: 1634 4472
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2631 2663 4175 4207 5688 5720 2631
Size: 32 1512 32 1481 32 3017
Fragments arranged by size:
3017 1512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 6106
PshAI GACnn'nnGTC
Cuts at: 1613 3075 1613
Size: 1462 4644
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3077 3458 830
Size: 2247 381 3478
Fragments arranged by size:
3478 2247 381
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 2109 2109
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SfcI C'TryA_G
Cuts at: 826 1045 2935 3073 3454 4589 5270 5461 826
Size: 219 1890 138 381 1135 681 191 1471
Fragments arranged by size:
1890 1471 1135 681 381 219 191 138
SfoI GGC'GCC
Cuts at: 2446 2446
Size: 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3317 4210 5111 5373 820
Size: 197 926 774 600 893 901 262 1553
Fragments arranged by size:
1553 926 901 893 774 600 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 2419 4030 5
Size: 47 2367 1611 2081
Fragments arranged by size:
2367 2081 1611 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2004 2216 3545 513
Size: 552 939 212 1329 3074
Fragments arranged by size:
3074 1329 939 552 212
SwaI ATTT'AAAT
Cuts at: 2067 2067
Size: 6106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2689 2852 2974 4133 4150 4303 4488
Size: 1255 1109 163 122 1159 17 153 185
Cuts at: 4488 4933 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1159 1109 445 185 163 153
122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2010 3024 4352
Size: 274 80 33 51 1443 33 1014 1328
Cuts at: 4352 6064 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1328 1014 274 138 80 51
33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6066 6098 6066
Size: 32 6074
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SrfI XcmI