Restriction map for
pFN21AB0409
(Circular) (Six-base) MAPSORT of: pFN21AB0409.seq Check: 2549 from: 1 to: 6103
pFN21AB0409 6103 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3252 3424 3252
Size: 172 5931
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 4166 5550 5818 1632
Size: 401 2133 1384 268 1917
Fragments arranged by size:
2133 1917 1384 401 268
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 6103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 2190 3162 5002 5979 1203
Size: 987 972 1840 977 1327
Fragments arranged by size:
1840 1327 987 977 972
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 2557 4832 2557
Size: 2275 3828
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 6071
AlwNI CAG_nnn'CTG
Cuts at: 2689 3033 3046 3846 5418 2689
Size: 344 13 800 1572 3374
Fragments arranged by size:
3374 1572 800 344 13
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2182 2182
Size: 6103
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 2890 3428 1870
Size: 73 322 625 538 4545
Fragments arranged by size:
4545 625 538 322 73
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2175 2504 2523 2894 2959
Size: 325 217 627 388 329 19 371 65
Cuts at: 2959 3056 3252 3424 4879 618
Size: 97 196 172 1455 1842
Fragments arranged by size:
1842 1455 627 388 371 329 325 217
196 172 97 65 19
BanII G_rGCy'C
Cuts at: 1391 2219 2363 2710 3075 3394 3422 1391
Size: 828 144 347 365 319 28 4072
Fragments arranged by size:
4072 828 365 347 319 144 28
BbeI G_GCGC'C
Cuts at: 2179 3060 2179
Size: 881 5222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 928
Size: 971 229 4903
Fragments arranged by size:
4903 971 229
BbvCI CC'TCA_GC
Cuts at: 2822 2822
Size: 6103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 2730 1548
Size: 1182 4921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4714 136
Size: 107 122 71 631 605 3042 1525
Fragments arranged by size:
3042 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2271 6098 2271
Size: 3827 2276
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 2507 2663 2897 2964 3093 3248 4164 5320
Size: 717 156 234 67 129 155 916 1156
Cuts at: 5320 1790
Size: 2573
Fragments arranged by size:
2573 1156 916 717 234 156 155 129
67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3322 3834 4792
Size: 416 53 83 399 24 1905 512 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1905 1753 958 512 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2554 2266
Size: 288 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2243 2363 2806 3089 4763 1239
Size: 980 24 120 443 283 1674 2579
Fragments arranged by size:
2579 1674 980 443 283 120 24
Bpu10I CC'TnA_GC
Cuts at: 2079 2822 2079
Size: 743 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 4228 5093 5391 2323
Size: 1905 865 298 3035
Fragments arranged by size:
3035 1905 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 2755 3328 4766 882
Size: 617 515 741 573 1438 2219
Fragments arranged by size:
2219 1438 741 617 573 515
BsaAI yAC'GTr
Cuts at: 493 1204 2883 3249 3894 493
Size: 711 1679 366 645 2702
Fragments arranged by size:
2702 1679 711 645 366
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2176 3057 3910
Size: 53 83 186 936 630 13 881 853
Cuts at: 3910 4292 4927 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 881 853 635 630 382 186
83 53 13
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2216 2231 2246 2261 3149
Size: 30 165 30 813 15 15 15 888
Cuts at: 3149 3179 1178
Size: 30 4102
Fragments arranged by size:
4102 888 813 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 2106 2216 2255 2273 1186
Size: 832 88 110 39 18 5016
Fragments arranged by size:
5016 832 110 88 39 18
BseYI C'CCAG_C
Cuts at: 2806 2876 3033 3273 5306 5802 2806
Size: 70 157 240 2033 496 3107
Fragments arranged by size:
3107 2033 496 240 157 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2548 2912 1163
Size: 573 417 395 364 4354
Fragments arranged by size:
4354 573 417 395 364
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2213 3553 4314 5342 1540
Size: 275 186 212 1340 761 1028 2301
Fragments arranged by size:
2301 1340 1028 761 275 212 186
BsiHKAI G_wGCw'C
Cuts at: 2252 2710 2817 2832 3075 4164 4249 5320 2252
Size: 458 107 15 243 1089 85 1071 3035
Fragments arranged by size:
3035 1089 1071 458 243 107 85 15
BsiWI C'GTAC_G
Cuts at: 2883 2883
Size: 6103
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
BsmBI CGTCTCn'nnnn_
Cuts at: 2423 2423
Size: 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2252 2363 2507 2663 2710 2817
Size: 399 429 33 111 144 156 47 107
Cuts at: 2817 2832 2897 2964 3075 3093 3248 3394 3422
Size: 15 65 67 111 18 155 146 28
Cuts at: 3422 4164 4249 5320 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 156 155 146
144 111 111 107 85 67 65 47
33 28 18 15
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2674 2854 3458 844
Size: 1830 180 604 3489
Fragments arranged by size:
3489 1830 604 180
BsrBI CCG'CTC
Cuts at: 1394 1823 3990 1394
Size: 429 2167 3507
Fragments arranged by size:
3507 2167 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2172 2178 2850 3173 4747 4934 1700
Size: 110 362 6 672 323 1574 187 2869
Fragments arranged by size:
2869 1574 672 362 323 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 3312 96
Size: 3216 2887
BssHII G'CGCG_C
Cuts at: 1925 2182 1925
Size: 257 5846
BssSI C'ACGA_G
Cuts at: 2528 4163 5175 2528
Size: 1635 1012 3456
Fragments arranged by size:
3456 1635 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2658 794
Size: 1864 4239
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2746 3067 2746
Size: 321 5782
BstYI r'GATC_y
Cuts at: 1972 2271 3433 3800 4185 4202 5654 5740 5752
Size: 299 1162 367 385 17 1452 86 12
Cuts at: 5752 6098 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1162 385 367 346 299 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2021 2086 2021
Size: 65 6038
BtgI C'CryG_G
Cuts at: 513 1065 2004 2111 2158 2678 2754 2937 3052
Size: 552 939 107 47 520 76 183 115
Cuts at: 3052 3060 3294 513
Size: 8 234 3322
Fragments arranged by size:
3322 939 552 520 234 183 115 107
76 47 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2896 487
Size: 683 56 1670 3694
Fragments arranged by size:
3694 1670 683 56
BtsI GCAGTG_nn'
Cuts at: 2252 2497 3619 4413 4433 2252
Size: 245 1122 794 20 3922
Fragments arranged by size:
3922 1122 794 245 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2210 3550 4439
Size: 54 1425 50 275 18 380 1340 889
Cuts at: 4439 6068 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1340 889 380 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2210 3550 1537
Size: 275 398 1340 4090
Fragments arranged by size:
4090 1340 398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4033 1353
Size: 2680 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2912 4690 5064 5210 387
Size: 1064 138 1323 1778 374 146 1280
Fragments arranged by size:
1778 1323 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2708 3073 2708
Size: 365 5738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2219 2243 2363 2806 3089 4166
Size: 393 401 186 24 120 443 283 1077
Cuts at: 4166 4763 5550 5818 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1077 787 597 443 401 393 283
268 186 120 24
EcoO109I rG'GnC_Cy
Cuts at: 2017 2123 2686 2724 2017
Size: 106 563 38 5396
Fragments arranged by size:
5396 563 106 38
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FspI TGC'GCA
Cuts at: 3289 4609 3289
Size: 1320 4783
HaeII r_GCGC'y
Cuts at: 1678 2179 2515 3060 5250 1678
Size: 501 336 545 2190 2531
Fragments arranged by size:
2531 2190 545 501 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2543 2575 4744 4776 4818
Size: 32 135 32 971 32 2169 32 42
Cuts at: 4818 4850 1373
Size: 32 2626
Fragments arranged by size:
2626 2169 971 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 3693 669
Size: 988 281 1509 246 3079
Fragments arranged by size:
3079 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KasI G'GCGC_C
Cuts at: 2175 3056 2175
Size: 881 5222
KpnI G_GTAC'C
Cuts at: 3256 3428 3256
Size: 172 5931
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 2190 5979 2190
Size: 3789 2314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5217 5401 1305
Size: 3912 184 2007
Fragments arranged by size:
3912 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4281
Fragments arranged by size:
4281 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 2744 4061 4420 4579 518
Size: 648 1273 305 1317 359 159 2042
Fragments arranged by size:
2042 1317 1273 648 359 305 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2756 3030 3043 3273 3519 4196 5344
Size: 843 307 274 13 230 246 677 1148
Cuts at: 5344 5589 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 843 677 307 274 246 245
230 13
NaeI GCC'GGC
Cuts at: 2180 2852 3175 2180
Size: 672 323 5108
Fragments arranged by size:
5108 672 323
NarI GG'CG_CC
Cuts at: 2176 3057 2176
Size: 881 5222
NcoI C'CATG_G
Cuts at: 513 1065 2004 2678 513
Size: 552 939 674 3938
Fragments arranged by size:
3938 939 674 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NgoMIV G'CCGG_C
Cuts at: 2178 2850 3173 2178
Size: 672 323 5108
Fragments arranged by size:
5108 672 323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 2110 3166 3230 3461 5006 1132
Size: 97 881 1056 64 231 1545 2229
Fragments arranged by size:
2229 1545 1056 881 231 97 64
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6103
PciI A'CATG_T
Cuts at: 3162 5002 3162
Size: 1840 4263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2938 3046 1108
Size: 1830 108 4165
Fragments arranged by size:
4165 1830 108
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PmlI CAC'GTG
Cuts at: 3249 3249
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 4172 4204 5685 5717 3149
Size: 32 991 32 1481 32 3535
Fragments arranged by size:
3535 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2686 2724 2686
Size: 38 6065
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PstI C_TGCA'G
Cuts at: 830 2068 2492 3120 3455 830
Size: 1238 424 628 335 3478
Fragments arranged by size:
3478 1238 628 424 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2449 3043 3273 2449
Size: 594 230 5279
Fragments arranged by size:
5279 594 230
SacI G_AGCT'C
Cuts at: 2710 3075 2710
Size: 365 5738
SacII CC_GC'GG
Cuts at: 2757 2757
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SanDI GG'GwC_CC
Cuts at: 2724 2724
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 2486 4351 6063 2486
Size: 1865 1712 2526
Fragments arranged by size:
2526 1865 1712
SfcI C'TryA_G
Cuts at: 826 1045 2064 2488 3116 3451 4586 5267 5458
Size: 219 1019 424 628 335 1135 681 191
Cuts at: 5458 826
Size: 1471
Fragments arranged by size:
1471 1135 1019 681 628 424 335 219
191
SfoI GGC'GCC
Cuts at: 2177 3058 2177
Size: 881 5222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 2172 2178 1700
Size: 472 6 5625
Fragments arranged by size:
5625 472 6
SmaI CCC'GGG
Cuts at: 1872 2267 2892 3430 1872
Size: 395 625 538 4545
Fragments arranged by size:
4545 625 538 395
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 4207 5108 5370 820
Size: 197 926 395 1869 901 262 1553
Fragments arranged by size:
1869 1553 926 901 395 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 2084 1517
Size: 567 5536
StyI C'CwwG_G
Cuts at: 513 1065 2004 2632 2678 3542 513
Size: 552 939 628 46 864 3074
Fragments arranged by size:
3074 939 864 628 552 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2584 2734 3248 4130 4147 4300 4485
Size: 1255 1004 150 514 882 17 153 185
Cuts at: 4485 4930 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1004 882 514 445 185 153
150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2484 3159 3312
Size: 274 80 33 51 1443 507 675 153
Cuts at: 3312 3330 4349 6061 96
Size: 18 1019 1712 138
Fragments arranged by size:
1712 1443 1019 675 507 274 153 138
80 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 2216 2231 2248 2263 6063 6095 2096
Size: 32 88 15 17 15 3800 32 2104
Fragments arranged by size:
3800 2104 88 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 2605 3439 2605
Size: 834 5269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 2265 2890 3428 1870
Size: 395 625 538 4545
Fragments arranged by size:
4545 625 538 395
XmnI GAAnn'nnTTC
Cuts at: 2300 4232 2300
Size: 1932 4171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AvrII BaeI BfrBI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI